Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were r...Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were randomly divided into (1) normal control group, (2) DSS group, (3) DSS + SASP treatment group, (4) DSS + PP treatment group. After the experiment, the mice in each group were observed daily general conditions, Immunohistochemistry was used to detect the expression of ERK, p38 MAPK and JNK protein in colonic mucosa of mice in each group. The expression of TNF-_and IL-6 cytokines in colonic tissue of mice was detected by ELASA. Results: The general situation of SASP group and PP group was better than DSS group;Compared with DSS group, the expression of ERK, p38 MAPK and JNK protein was significantly decreased. The levels of TNF-a and IL-6 in colon tissue of SASP group and PP group were significantly decreased. Conclusion: The results suggest that pectin polysaccharide from Rauvolfia hainanensis extract has a significant therapeutic effect on colitis in DSS mice, which may via regulating the MAPK signal pathway pathways.展开更多
通过对专著、文献和多个网络资源数据库(植物志、标本、图像等)分析考证,及赴部分产地的野外实地考察,对珍稀濒危植物驼峰藤(Merrillanthus hainanensis Chun et Tsiang)的分类地位、形态特征、标本记录与保存、产地与生境、濒危的野外...通过对专著、文献和多个网络资源数据库(植物志、标本、图像等)分析考证,及赴部分产地的野外实地考察,对珍稀濒危植物驼峰藤(Merrillanthus hainanensis Chun et Tsiang)的分类地位、形态特征、标本记录与保存、产地与生境、濒危的野外生存现状等情况进行了综述,以期为该保护物种的生存和发展和保护提供参考。自1933年采集到标本和1941年命名驼峰藤以来,至今已有80多年的历史,研究表明它的分布区范围从原广东、海南省扩展到中国大陆西南部和中南半岛部分地区(缅甸和柬埔寨),部分原有记载分布点已无法找到野生植株,导致其濒危的主要原因是生境的破坏和质量下降,广东中山五桂山脉或许是目前发现的最大野生种群保存地,文中就其保护措施提出了相关建议。展开更多
基金National Natural Science Foundation of China(No.81360603)Natural Science Foundation of Hainan Province(No.813215).
文摘Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were randomly divided into (1) normal control group, (2) DSS group, (3) DSS + SASP treatment group, (4) DSS + PP treatment group. After the experiment, the mice in each group were observed daily general conditions, Immunohistochemistry was used to detect the expression of ERK, p38 MAPK and JNK protein in colonic mucosa of mice in each group. The expression of TNF-_and IL-6 cytokines in colonic tissue of mice was detected by ELASA. Results: The general situation of SASP group and PP group was better than DSS group;Compared with DSS group, the expression of ERK, p38 MAPK and JNK protein was significantly decreased. The levels of TNF-a and IL-6 in colon tissue of SASP group and PP group were significantly decreased. Conclusion: The results suggest that pectin polysaccharide from Rauvolfia hainanensis extract has a significant therapeutic effect on colitis in DSS mice, which may via regulating the MAPK signal pathway pathways.
文摘通过对专著、文献和多个网络资源数据库(植物志、标本、图像等)分析考证,及赴部分产地的野外实地考察,对珍稀濒危植物驼峰藤(Merrillanthus hainanensis Chun et Tsiang)的分类地位、形态特征、标本记录与保存、产地与生境、濒危的野外生存现状等情况进行了综述,以期为该保护物种的生存和发展和保护提供参考。自1933年采集到标本和1941年命名驼峰藤以来,至今已有80多年的历史,研究表明它的分布区范围从原广东、海南省扩展到中国大陆西南部和中南半岛部分地区(缅甸和柬埔寨),部分原有记载分布点已无法找到野生植株,导致其濒危的主要原因是生境的破坏和质量下降,广东中山五桂山脉或许是目前发现的最大野生种群保存地,文中就其保护措施提出了相关建议。
文摘从海南蕊木(Kopsia hainanensis Tsiang)枝叶的乙醇提取物中分离得到7个化合物,通过波谱分析(NMR、MS、IR等),鉴定其结构分别为:蕊木宁(1)、土波台文碱(2)、二氢脱氢二松柏基醇(3)、β-香树脂醇(4)、熊果酸(5)、2α,3β-二羟基熊果酸(6)和β-谷甾醇(7)。其中化合物3～7为首次从该植物中分离得到。用滤纸片琼脂扩散法检测表明,化合物1～3和5对耐甲氧西林金黄色葡萄球菌(MRSA)生长有抑制作用;化合物2对金黄色葡萄球菌生长有抑制作用;化合物5对慢性髓原白血病细胞(K562)、人胃癌细胞(SGC-7901)和人肝癌细胞(SM M C-7721)增殖均具有细胞毒活性。
