Sp1/Krüppel样因子(Sp1-like and Krüppel-like factors,Sp1/KLFs)是一组与真核细胞转录调控密切相关的锌指蛋白。Sp1/KLFs的羧基末端高度保守,含有3个串联的Cys2His2锌指结构,用于结合DNA;其氨基末端在不同的家族成员间存在...Sp1/Krüppel样因子(Sp1-like and Krüppel-like factors,Sp1/KLFs)是一组与真核细胞转录调控密切相关的锌指蛋白。Sp1/KLFs的羧基末端高度保守,含有3个串联的Cys2His2锌指结构,用于结合DNA;其氨基末端在不同的家族成员间存在较大差异,主要是通过结合辅助因子发挥转录调控作用。Sp1/KLFs的表达具有组织、细胞分布以及发育时期的特异性,它们通过调控多种富含GC或CACCC的启动子的基因的表达,参与细胞增殖、分化、凋亡和肿瘤发生、发展等多种生理、病理过程。文章综述了Sp1/KLFs的结构特征、作用机制及生物学功能。展开更多
Kr1基因是小麦远缘杂交不亲和的主效基因。为探究Kr1基因的特性,根据已公布的Kr1基因c DNA部分序列,采用染色体步移(Genome Walking)和c DNA末端快速扩增技术(rapid amplification of c DNA ends,RACE)克隆小麦显性Kr1基因和隐性kr1基因...Kr1基因是小麦远缘杂交不亲和的主效基因。为探究Kr1基因的特性,根据已公布的Kr1基因c DNA部分序列,采用染色体步移(Genome Walking)和c DNA末端快速扩增技术(rapid amplification of c DNA ends,RACE)克隆小麦显性Kr1基因和隐性kr1基因,并进行序列分析。结果表明,小麦Kr1基因全长4 006 bp,含有4个外显子和3个内含子,ORF全长1 671 bp,编码557个氨基酸,可形成一条完整肽链,有基因活性,Kr1蛋白三级结构与植物S位点受体激酶具有较高的相似性。kr1基因全长3 945bp,第3、第4外显子中因含有大量终止密码子,不能通读,表明kr1基因无基因活性。同时对小麦×黑麦、小麦×球茎大麦、小麦×玉米等不同远缘杂交系统的亲和性差异进行探讨,认为Kr1基因在小麦×玉米中失活可能与玉米转座子的插入有关。本研究结果为进一步阐明Kr1基因的功能及其作用机制奠定了基础。展开更多
The brown planthopper, Nilaparvata lugens（Stl）, is the most serious insect pest of rice. It has developed high resistance to traditional insecticides because of their intensive use. Juvenile hormone（JH） analogs ha...The brown planthopper, Nilaparvata lugens（Stl）, is the most serious insect pest of rice. It has developed high resistance to traditional insecticides because of their intensive use. Juvenile hormone（JH） analogs have been used successfully to control this species and other pest insects. However, the molecular mechanism of JH signaling is not well understood. Krüppel-homolog 1（Kr-h1） is a transcription factor involved in the JH pathway. In this study, the Kr-h1 cDNA was cloned and characterized from N. lugens by rapid amplification of cDNA ends（RACE） and reverse transcription PCR（RT-PCR）. Its spatial and temporal expression profiles were examined by real-time quantitative PCR, and its function was also studied by RNA interference（RNAi）. The open reading frame of NlKr-h1 is 1 833 bp encoding for 611 amino acids. The protein contains eight conserved zinc-finger motifs. NlKr-h1 was expressed at all life stages, with the highest mRNA level in the 4-day embryo. NlKr-h1 mRNA levels rose during each nymphal molt after the 2nd instar. In the adults, the mRNA level in males was significantly higher than that in females of either the macropterous or brachypterous type. The highest expression was observed in the female midgut. NlKr-h1 was activated by juvenile hormone III（JH III） in the 3rd-5th instar nymphs. Disruption of Nlkr-h1 expression by RNAi caused stunted wing development and malformations of both male and female external genitalia. Our findings suggest that Kr-h1 may be a useful target for pest insect management.展开更多
文摘Sp1/Krüppel样因子(Sp1-like and Krüppel-like factors,Sp1/KLFs)是一组与真核细胞转录调控密切相关的锌指蛋白。Sp1/KLFs的羧基末端高度保守,含有3个串联的Cys2His2锌指结构,用于结合DNA;其氨基末端在不同的家族成员间存在较大差异,主要是通过结合辅助因子发挥转录调控作用。Sp1/KLFs的表达具有组织、细胞分布以及发育时期的特异性,它们通过调控多种富含GC或CACCC的启动子的基因的表达,参与细胞增殖、分化、凋亡和肿瘤发生、发展等多种生理、病理过程。文章综述了Sp1/KLFs的结构特征、作用机制及生物学功能。
文摘Kr1基因是小麦远缘杂交不亲和的主效基因。为探究Kr1基因的特性,根据已公布的Kr1基因c DNA部分序列,采用染色体步移(Genome Walking)和c DNA末端快速扩增技术(rapid amplification of c DNA ends,RACE)克隆小麦显性Kr1基因和隐性kr1基因,并进行序列分析。结果表明,小麦Kr1基因全长4 006 bp,含有4个外显子和3个内含子,ORF全长1 671 bp,编码557个氨基酸,可形成一条完整肽链,有基因活性,Kr1蛋白三级结构与植物S位点受体激酶具有较高的相似性。kr1基因全长3 945bp,第3、第4外显子中因含有大量终止密码子,不能通读,表明kr1基因无基因活性。同时对小麦×黑麦、小麦×球茎大麦、小麦×玉米等不同远缘杂交系统的亲和性差异进行探讨,认为Kr1基因在小麦×玉米中失活可能与玉米转座子的插入有关。本研究结果为进一步阐明Kr1基因的功能及其作用机制奠定了基础。
基金supported by the National 973 Program of China(2010CB126205)the Zhejiang Provincial Natural Science Foundation of China(LY14C140002)
文摘The brown planthopper, Nilaparvata lugens（Stl）, is the most serious insect pest of rice. It has developed high resistance to traditional insecticides because of their intensive use. Juvenile hormone（JH） analogs have been used successfully to control this species and other pest insects. However, the molecular mechanism of JH signaling is not well understood. Krüppel-homolog 1（Kr-h1） is a transcription factor involved in the JH pathway. In this study, the Kr-h1 cDNA was cloned and characterized from N. lugens by rapid amplification of cDNA ends（RACE） and reverse transcription PCR（RT-PCR）. Its spatial and temporal expression profiles were examined by real-time quantitative PCR, and its function was also studied by RNA interference（RNAi）. The open reading frame of NlKr-h1 is 1 833 bp encoding for 611 amino acids. The protein contains eight conserved zinc-finger motifs. NlKr-h1 was expressed at all life stages, with the highest mRNA level in the 4-day embryo. NlKr-h1 mRNA levels rose during each nymphal molt after the 2nd instar. In the adults, the mRNA level in males was significantly higher than that in females of either the macropterous or brachypterous type. The highest expression was observed in the female midgut. NlKr-h1 was activated by juvenile hormone III（JH III） in the 3rd-5th instar nymphs. Disruption of Nlkr-h1 expression by RNAi caused stunted wing development and malformations of both male and female external genitalia. Our findings suggest that Kr-h1 may be a useful target for pest insect management.
