Theory is considered essential for integral assessment, adopting its foundations and concepts is of great utility. The theory provides different templates to help nurses provide care that respects patients and improve...Theory is considered essential for integral assessment, adopting its foundations and concepts is of great utility. The theory provides different templates to help nurses provide care that respects patients and improves outcomes. Through understanding the intersection of nursing, patients, health, and the environment, theories aim to simplify the complicated, ever-evolving relationship that nurses have with their profession. Nursing theory helps distinguish nursing as a separate discipline from medicine and related sciences, and assists nurses in understanding their patients and their needs. The behaviors of healthcare providers affect how patients participate in and experience care situations. In the nursing discipline, the theoretical structures of caring have been established as the core concept of guidance in all nurses’ work. The aim of this paper is to develop a critique of Kristen Swanson’s theory of caring—a theory structured around five caring principles (maintaining belief, knowing, being with, doing for, and enabling) by applying it to nursing practice. When applied to nursing practice, each of these five stages stimulates the caregiver’s attitude, which in turn improves the overall patient’s well-being. Implications to nursing practice are mentioned [1] [2].展开更多
目的通过对中国人结肠癌染色体12p12-13杂合性丢失(loss of heterozygosity,LOH)分析,了解KRAS2基因与结肠癌发生发展的关系。方法从10例结肠癌手术切除标本中,提取肿瘤、癌旁及相应正常组织的DNA,用PCR-变性聚丙烯酰胺凝胶电泳-免疫荧...目的通过对中国人结肠癌染色体12p12-13杂合性丢失(loss of heterozygosity,LOH)分析,了解KRAS2基因与结肠癌发生发展的关系。方法从10例结肠癌手术切除标本中,提取肿瘤、癌旁及相应正常组织的DNA,用PCR-变性聚丙烯酰胺凝胶电泳-免疫荧光标记多重微卫星PCR法,对染色体12p12-13杂合性丢失进行分析。结果10例癌旁组织中有3例(30%)在12p12-13处至少有一个位点出现杂合性丢失,其中D12S1034出现频率最高,为28.57%(2/7);10例原发性结肠癌标本中6例(60%)在12p12-13处至少有一个位点出现有杂合性丢失,D12S1034和D12S1591是高频率丢失集中的区域,均占42.86%(3/7);癌旁组织、癌组织均发生LOH的标本有3例,占30%(3/10);仅在癌组织中发生LOH的标本有3例,占30%(3/10);仅在癌旁组织中发生LOH,癌组织无信号的标本1例;杂合性丢失的频率与肿瘤患者的年龄、性别、肿瘤大小、肿瘤部位及淋巴结转移均无关。结论KRAS2基因所在的12p12-13区域在结肠癌发生发展过程中出现基因组不稳定,该区域的高频杂合性丢失可能直接影响野生型KRAS2基因的转录与翻译。展开更多
文摘Theory is considered essential for integral assessment, adopting its foundations and concepts is of great utility. The theory provides different templates to help nurses provide care that respects patients and improves outcomes. Through understanding the intersection of nursing, patients, health, and the environment, theories aim to simplify the complicated, ever-evolving relationship that nurses have with their profession. Nursing theory helps distinguish nursing as a separate discipline from medicine and related sciences, and assists nurses in understanding their patients and their needs. The behaviors of healthcare providers affect how patients participate in and experience care situations. In the nursing discipline, the theoretical structures of caring have been established as the core concept of guidance in all nurses’ work. The aim of this paper is to develop a critique of Kristen Swanson’s theory of caring—a theory structured around five caring principles (maintaining belief, knowing, being with, doing for, and enabling) by applying it to nursing practice. When applied to nursing practice, each of these five stages stimulates the caregiver’s attitude, which in turn improves the overall patient’s well-being. Implications to nursing practice are mentioned [1] [2].
文摘目的通过对中国人结肠癌染色体12p12-13杂合性丢失(loss of heterozygosity,LOH)分析,了解KRAS2基因与结肠癌发生发展的关系。方法从10例结肠癌手术切除标本中,提取肿瘤、癌旁及相应正常组织的DNA,用PCR-变性聚丙烯酰胺凝胶电泳-免疫荧光标记多重微卫星PCR法,对染色体12p12-13杂合性丢失进行分析。结果10例癌旁组织中有3例(30%)在12p12-13处至少有一个位点出现杂合性丢失,其中D12S1034出现频率最高,为28.57%(2/7);10例原发性结肠癌标本中6例(60%)在12p12-13处至少有一个位点出现有杂合性丢失,D12S1034和D12S1591是高频率丢失集中的区域,均占42.86%(3/7);癌旁组织、癌组织均发生LOH的标本有3例,占30%(3/10);仅在癌组织中发生LOH的标本有3例,占30%(3/10);仅在癌旁组织中发生LOH,癌组织无信号的标本1例;杂合性丢失的频率与肿瘤患者的年龄、性别、肿瘤大小、肿瘤部位及淋巴结转移均无关。结论KRAS2基因所在的12p12-13区域在结肠癌发生发展过程中出现基因组不稳定,该区域的高频杂合性丢失可能直接影响野生型KRAS2基因的转录与翻译。
