Kruppel-like factor 4(Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis.However, its regulation during embryogenesis is still unclear. Here, we report that Tcf711, a key down...Kruppel-like factor 4(Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis.However, its regulation during embryogenesis is still unclear. Here, we report that Tcf711, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf711. Moreover, the dominant negative form of Tcf711(dnTcf711), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent.Taken together, our results demonstrate that Tcf711 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.展开更多
目的探讨人肝细胞癌中核转录因子Kruppel样因子4(KLF4)与叉头框蛋白M1(FOXM1)基因表达相关性,以及两种转录因子表达对肝细胞癌生长和增殖的影响。方法采用实时荧光定量PCR技术检测50例肝细胞癌患者肝癌组织及8株肝癌细胞中KLF4m RNA和FO...目的探讨人肝细胞癌中核转录因子Kruppel样因子4(KLF4)与叉头框蛋白M1(FOXM1)基因表达相关性,以及两种转录因子表达对肝细胞癌生长和增殖的影响。方法采用实时荧光定量PCR技术检测50例肝细胞癌患者肝癌组织及8株肝癌细胞中KLF4m RNA和FOXM1 m RNA表达水平并分析其相关性;KLF4过表达质粒和KLF4 si RNA分别转染人肝癌细胞Hep3B和BEL-7402,采用实时荧光定量PCR、蛋白印迹实验检测KLF4 m RNA、FOXM1m RNA及KLF4蛋白、FOXM1蛋白表达水平;通过CCK8实验检测KLF4和FOXM1基因对细胞增殖的影响。结果肝癌组织和细胞中KLF4低表达,而FOXM1高表达,两者呈负相关(r=-0.462,P=0.001);KLF4基因过表达抑制肝癌细胞增殖,而FOXM1基因过表达促进肝癌细胞增殖,FOXM1基因过表达能恢复KLF4对肝癌细胞抑制增殖作用。结论在肝细胞癌中,KLF4和FOXM1基因表达呈负相关,两者不同的表达水平可影响肝癌细胞的增殖。展开更多
基金supported by the Start-up Funding of Henan University of Science and Technology(13480027) to Q. C.the Key Science Foundation of Nanjing Medical University(2015NJMUZD002)+2 种基金the Natural Science Foundation of Higher Education Institutions of Jiangsu Province(16KJB-180020)Natural Science Foundation of Jiangsu Province (BK20171053)National Natural Science Funds of China (81702747) to C.L
文摘Kruppel-like factor 4(Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis.However, its regulation during embryogenesis is still unclear. Here, we report that Tcf711, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf711. Moreover, the dominant negative form of Tcf711(dnTcf711), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent.Taken together, our results demonstrate that Tcf711 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.
文摘目的探讨人肝细胞癌中核转录因子Kruppel样因子4(KLF4)与叉头框蛋白M1(FOXM1)基因表达相关性,以及两种转录因子表达对肝细胞癌生长和增殖的影响。方法采用实时荧光定量PCR技术检测50例肝细胞癌患者肝癌组织及8株肝癌细胞中KLF4m RNA和FOXM1 m RNA表达水平并分析其相关性;KLF4过表达质粒和KLF4 si RNA分别转染人肝癌细胞Hep3B和BEL-7402,采用实时荧光定量PCR、蛋白印迹实验检测KLF4 m RNA、FOXM1m RNA及KLF4蛋白、FOXM1蛋白表达水平;通过CCK8实验检测KLF4和FOXM1基因对细胞增殖的影响。结果肝癌组织和细胞中KLF4低表达,而FOXM1高表达,两者呈负相关(r=-0.462,P=0.001);KLF4基因过表达抑制肝癌细胞增殖,而FOXM1基因过表达促进肝癌细胞增殖,FOXM1基因过表达能恢复KLF4对肝癌细胞抑制增殖作用。结论在肝细胞癌中,KLF4和FOXM1基因表达呈负相关,两者不同的表达水平可影响肝癌细胞的增殖。
