对不同方法提取苦丁茶多酚的比较试验

本文通过使用不同的提取溶剂,不同的提取时间,对从苦丁茶中提取茶多酚进行了研究。另外,本文对大叶苦丁茶和小叶苦丁茶中所含茶多酚进行了比较。在溶剂浸提中,从成品小叶苦丁茶中提取多酚,提取质量以70%乙醇作溶剂最多,其次为95%、50%、40%,从水中提取最少;多酚含量同样是70%乙醇作溶剂最高,以水作溶剂最低。在成品大叶苦丁茶中,提取质量随浓度的增高而增多,而多酚含量也同样呈递增的趋势。在新鲜大叶苦丁茶中,提取质量以95%为最高,其次为60%、80%、50%,水中提取最少;而多酚含量则呈递增趋势。采用相同的溶剂提取与分析方法,苦丁茶提取物的质量:成品小叶>成品大叶>新鲜大叶;多酚含量则:成品大叶>新鲜大叶>成品小叶。

苦丁茶冬青叶化学成分及药理作用研究进展

苦丁茶冬青叶(苦丁茶)是民间常用中草药,主要用于治疗头痛、齿痛、目赤、热病烦渴、痢疾等症。本文就近年来国内外对苦丁茶冬青叶的化学成分及药理作用的研究进展作一综述。

苦丁茶的本草及植物来源考证

根据古代、近代及现代文献记载 ,对苦丁茶进行本草及植物来源考证 ,认为苦丁茶植物来源有三 :一是冬青科冬青属为主的枸骨、大叶冬青、苦丁茶冬青等 ;二是以木犀科女贞属植物为主的变紫女贞、女贞、日本毛女贞等 ;三是上述两类的混淆品 ,如木犀科牛矢果等。

HPLC法测定川产苦丁茶中有效成分齐墩果酸的含量

目的 采用HPLC法测定川产苦丁茶中有效成分之一齐墩果酸的含量。方法 使用C1 8色谱柱 ,流动相为甲醇 -水 (85∶1 5 ) ,流速 1mL/min ,检测波长为 2 1 0nm ,柱温 2 5℃。结果 齐墩果酸的进样量在 0 4 36 0～ 3 0 5 2ug范围内峰面积值与进样量之间线性关系良好 ,加样回收率为 99 1 % (n =6 )。紫茎女贞的齐墩果酸含量最高 ,为 0 6 6 9% ,RSD为 2 72 %。

三种天然提取物的减肥降脂作用研究

选用Wistar雄性大鼠,喂饲高脂饲料,建立预防性肥胖高血脂模型,对苦丁茶水提取物、阿拉伯糖、乌龙茶提取物三种天然成分进行减肥降脂试验。大鼠随机分组,灌胃给药,以药物曲美减肥胶囊为阳性对照组。测定模型组、给药实验组、阳性对照组各种生化指标(如总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白)、大鼠的体重、Lee′s指数和脂肪湿重等。结果表明:各给药实验组较模型对照组体重增长减慢,且下降明显,体内脂肪湿重也明显下降,同时发现L-阿拉伯糖组有极其显著降低血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、Lee′s指数和体内脂肪含量(P<0.01)的效果。L-阿拉伯糖对肥胖症、高血脂症具有良好的预防和治疗的作用。

小叶苦丁茶挥发油成分分析

[目的]分析比较不同品种或产地木犀科小叶苦丁茶老叶及芽中挥发油成分的异同。[方法]采用水蒸气蒸馏法分别对不同品种或产地的小叶苦丁茶中的挥发油进行提取,利用气质联用技术分析所提取的挥发油成分。[结果]共鉴定55个化合物,7个不同批次小叶苦丁茶老叶及芽中的成分具有较大的相似性,相对含量最高的均为以芳樟醇(48.34%～79.25%)为主的单萜类成分,其他含量较高的成分有α-松油醇、香叶醇何橙花醇等。[结论]该方法为小叶苦丁茶的质量控制和资源合理开发利用提供了科学依据。

In Vitro and In Vivo Evaluation of Antitumor Activity ofLigustrum robustum, A Chinese Herbal Tea

Objective: To examine the effect of the aqueous extract of Ligustrum robustum on tumor growth in vitro and in vivo and explore the possible molecular mechanisms. Methods: In in vitro study, cell viabilities of human cervical carcinoma cells(HeLa), human breast cancer cells(MCF-7), human prostate cancer cells(PC-3),human hepatoma cells(7721) and human colon carcinoma cells(SW480) were evaluated with cell counting kit-8.For L. robustum-treated Hela cells, early or late apoptosis were evaluated by annexin V/PI staining. Mitochondrial membrane potential was measured by staining cells with JC-1. Apoptosis was monitored by nuclear morphology based on chromatin condensation and fragmentation by 4’,6-diamidino-2-phenylinole(DAPI) staining. Caspase-3 and-8 activity levels were measured by a colorimetric assay. In vivo, to evaluate the possible mechanism of L. robustum-mediated antitumor effect, nude mouse xenograft study was also conducted. Results: In in vitro study, L. robustum was found to be toxic to HeLa, MCF-7, PC-3, 7721, SW480, with an half maximal inhibitory concentration value of 2–5 mg/mL(P<0.05). Moreover, externalization of phosphatidylserine, loss of mitochondrial membrane potential, DNA fragmentation and activation of caspase-3 and-8 were detected in L. robustumtreated Hela cells. Using a nude mouse model bearing Hela xenografts, we found that L. robustum reduced tumor volume and tumor weight(P<0.05), but had no effect on body weight and histological damage of important organs. Intraperitoneal injection of L. robustum caused a signi?cant reduction in serum aspartate transaminase and alanine transaminase levels(P<0.05). Furthermore, cleaved caspase-3-positive and terminal nucleotidyl transferase-mediated nick end labeling(TUNEL)-positive cells were observed in L. robustum-treated tumor tissues.Conclusions: L. robustum inhibits tumor cell growth both in vitro and in vivo by inducing apoptosis in a caspasedependent way without apparent hepatic toxicity and histological damage, which may offer partial scienti?c support for the ethnopharmacological claims of L. robustum as a herbal tea for its antitumor activity.