[Objective] The control methods of the bulb rot of Iphigenia indica Kunth.caused by Fusarium orthoceras App.et Wr.var.longius （Sherb.） were explored.[Method] The effect of eight different kinds of metal ions on the ...[Objective] The control methods of the bulb rot of Iphigenia indica Kunth.caused by Fusarium orthoceras App.et Wr.var.longius （Sherb.） were explored.[Method] The effect of eight different kinds of metal ions on the pathogen was studied;the contents of seven different kinds of metal ions in rhizosphere soil and bulb were also determined.[Result] The results showed that when the concentration of Cu2＋ or Mg2＋ was greater than 1.0mg/L or the concentration of Zn2＋ or Ca2＋ was lower than 0.5mg/L,the growth of pathogen could be inhibited.The results also showed that the bulb could enrich K highly,enrich Zn and Mg,limit the absorption of Ca and Fe,and inhibit the absorption of Cu and Mn during the growth process of bulb in I.indica Kunth.[Conclusion] Micronutrient fertilizer containing Mg could be used to prevent and cure the bulb rot of I.indica Kunth.展开更多
Mikania micrantha Kunth is an invasive alien weed and known as a plant killer around the world.Accurately and rapidly identifying M.micrantha in the wild is important for monitoring its growth status,as this helps man...Mikania micrantha Kunth is an invasive alien weed and known as a plant killer around the world.Accurately and rapidly identifying M.micrantha in the wild is important for monitoring its growth status,as this helps management officials to take the necessary steps to devise a comprehensive strategy to control the invasive weed in the identified area.However,this approach still mainly depends on satellite remote sensing and manual inspection.The cost is high and the accuracy rate and efficiency are low.We acquired color images of the monitoring area in the wild environment using an Unmanned Aerial Vehicle(UAV)and proposed a novel network-MmNet-based on a deep Convolutional Neural Network(CNN)to identify M.micrantha in the images.The network consists of AlexNet Local Response Normalization(LRN),along with the GoogLeNet and continuous convolution of VGG inception models.After training and testing,the identification of 400 testing samples by MmNet is very good,with accuracy of 94.50%and time cost of 10.369 s.Moreover,in quantitative comparative analysis,the proposed MmNet not only has high accuracy and efficiency but also simple construction and outstanding repeatability.Compared with recently popular CNNs,MmNet is more suitable for the identification of M.micrantha in the wild.However,to meet the challenge of wild environments,more M.micrantha images need to be acquired for MmNet training.In addition,the classification labels need to be sorted in more detail.Altogether,this research provides some theoretical and scientific basis for the development of intelligent monitoring and early warning systems for M.micrantha and other invasive species.展开更多
Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including...Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concen-tration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida:Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X12 – 3.210 X32 – 6.786 X42 – 7.290 X12X3 + 5.575 X1X32 – 4.843 X32X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.1725μg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.展开更多
Objective:To isolate,identify,and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida(L.)Kunth herbs.Methods:A dried sample of Peperomia pellucida herb was successively macerated with n-hex...Objective:To isolate,identify,and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida(L.)Kunth herbs.Methods:A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate.The ethyl acetate extract solution was evaporated to obtain the crude extract.Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds.Then,both compounds were elucidated and identified using the spectroscopic method.Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength.Results:Two bioactive compounds were successfully isolated from Peperomia pellucida herb,including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1 H-indene and pellucidin A.Both compounds demonstrated angiotensin-converting enzyme inhibitory activity,with IC50 values of 72 μM(27.95 μg/mL)and 1 1μM(4.4 μg/mL),respectively.Conclusions:In the present study,two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine,and support its use as an angiotensin-converting enzyme-inhibiting drug.展开更多
Piper auritum Kunth,a member of the Piperacea family,is a small shrub native to the Central American tropics.This plant was traditionally used in Latin American countries for the treatment of various medical condition...Piper auritum Kunth,a member of the Piperacea family,is a small shrub native to the Central American tropics.This plant was traditionally used in Latin American countries for the treatment of various medical conditions.Increase in the recent studies of P.auritum’s chemical components leads to the isolation of monoterpenoids,sesquiterpenoids,phenylpropanoids and several other compounds.Previous research has revealed that these secondary metabolites have diverse pharmacological activities including antidiabetic,antioxidant,anti-inflammatory,anti-pathogeny microorganism and repellent activity.This review summarizes relevant findings on P.auritum,and hopes to contribute to the exploration of its potentials for further development as an alternative medicine.展开更多
以苗族常用药材吉祥草(Reineckia carnea(Andr.)Kunth)为试材,采用盆栽控制试验,设置了对照(CK)、单独增二氧化碳(eC:CO_(2)浓度为(800±20)μmol·mol^(-1))、单独施氮(eN:施加5 g NH_(4)NO_(3))、同时增二氧化碳与施氮(eC+eN:...以苗族常用药材吉祥草(Reineckia carnea(Andr.)Kunth)为试材,采用盆栽控制试验,设置了对照(CK)、单独增二氧化碳(eC:CO_(2)浓度为(800±20)μmol·mol^(-1))、单独施氮(eN:施加5 g NH_(4)NO_(3))、同时增二氧化碳与施氮(eC+eN:CO_(2)浓度为(800±20)μmol·mol^(-1),且施加5 g NH_(4)NO_(3))4个处理,研究CO_(2)浓度升高、氮沉降及二者的交互作用对吉祥草的生长及其药用成分的影响,以期为吉祥草在药用生产上提供参考依据。结果表明:1)升高CO_(2)浓度和施氮或同时升高CO_(2)浓度和施氮均显著促进了吉祥草的形态生长,总生物量和可溶性蛋白质含量显著增加,茎中的碳含量显著降低,超氧化物歧化酶(SOD)活性无显著变化;2)施氮处理下全氮含量显著增加,过氧化氢酶(CAT)活性、根和叶中的皂苷含量显著下降,在此基础上升高CO_(2)浓度可缓解皂苷下降,CAT活性显著升高,内源激素含量在施氮或同时升高CO_(2)浓度和施氮处理下显著增加。综上所述,在实际生产过程中,可适当增加CO_(2)浓度,氮肥浓度不宜过高,有利于吉祥草的生长,并保证其药用品质不受损害。展开更多
目的观察吉祥草中甾体皂苷RCE-4对宫颈癌裸鼠移植瘤的抑制作用。方法采用传统方法分离鉴定RCE-4。将0.3 m L Caski(4×107/m L)细胞悬液接种于裸鼠右侧后腿背部皮下,建立宫颈癌移植瘤模型,然后按瘤体积大小随机分为模型组、紫杉醇(1...目的观察吉祥草中甾体皂苷RCE-4对宫颈癌裸鼠移植瘤的抑制作用。方法采用传统方法分离鉴定RCE-4。将0.3 m L Caski(4×107/m L)细胞悬液接种于裸鼠右侧后腿背部皮下,建立宫颈癌移植瘤模型,然后按瘤体积大小随机分为模型组、紫杉醇(10 mg/kg)和RCE-4(25、50、100 mg/kg)组,1次/d给药,连续4周,末次给药的次日处死裸鼠,称量裸鼠体质量、瘤质量,测量肿瘤体积,计算抑瘤率。光镜下观察移植瘤组织形态学变化,TUNEL染色测定细胞凋亡率,免疫组化检测肿瘤组织中COX-2表达,荧光定量PCR测定移植瘤组织中Bcl-2、Bax、Caspase-3和Caspase-9 mRNA表达,计算Bcl-2/Bax值,Western blot检测移植瘤组织中Survivin蛋白表达。结果 RCE-4(25、50、100 mg/kg)可明显降低宫颈癌裸鼠移植瘤的体积和瘤质量(P<0.05,P<0.01),上调Bax、Caspase-3和Caspase-9 mRNA表达,下调Bcl-2 mRNA表达和COX-2、Survivin蛋白表达及Bcl-2/Bax值(P<0.05,P<0.01)。结论RCE-4对宫颈癌裸鼠移植瘤的生长具有显著抑制作用,其机制可能与其上调Bax、Caspase-9、Caspase-3的表达和下调Survivin、Bcl-2、COX-2的表达有关。展开更多
目的建立吉祥草中阿魏酸和芦丁的测定方法。方法采用反相高效液相色谱法,Wondasil C18色谱柱（250mm×4.6mm,5μm）,流动相为甲醇-0.4%磷酸水（38∶62）,体积流量为1 m L/min,检测波长270nm;柱温30℃。结果阿魏酸在0.40-2.40μg范...目的建立吉祥草中阿魏酸和芦丁的测定方法。方法采用反相高效液相色谱法,Wondasil C18色谱柱（250mm×4.6mm,5μm）,流动相为甲醇-0.4%磷酸水（38∶62）,体积流量为1 m L/min,检测波长270nm;柱温30℃。结果阿魏酸在0.40-2.40μg范围内呈良好线性关系（r=0.999 9）,平均回收率为100.1%,RSD为2.8%;芦丁在0.625-5.625μg范围内呈良好线性关系（r=0.999 95）,平均回收率为100.7%,RSD为1.3%。结论该方法分离效果好,操作简单,稳定性好,重复性好,可用于吉祥草中阿魏酸和芦丁的测定。展开更多
文摘[Objective] The control methods of the bulb rot of Iphigenia indica Kunth.caused by Fusarium orthoceras App.et Wr.var.longius （Sherb.） were explored.[Method] The effect of eight different kinds of metal ions on the pathogen was studied;the contents of seven different kinds of metal ions in rhizosphere soil and bulb were also determined.[Result] The results showed that when the concentration of Cu2＋ or Mg2＋ was greater than 1.0mg/L or the concentration of Zn2＋ or Ca2＋ was lower than 0.5mg/L,the growth of pathogen could be inhibited.The results also showed that the bulb could enrich K highly,enrich Zn and Mg,limit the absorption of Ca and Fe,and inhibit the absorption of Cu and Mn during the growth process of bulb in I.indica Kunth.[Conclusion] Micronutrient fertilizer containing Mg could be used to prevent and cure the bulb rot of I.indica Kunth.
基金supported by the National Natural Science Foundation of China(3180111238)the Fund Project of the Key Laboratory of Integrated Pest Management on Crops in South China,Ministry of Agriculture and Rural Affairs,China(SCIPM2018-05)+2 种基金the Key Research and Development Program of Nanning,China(20192065)the Guangdong Science and Technology Planning Project,China(2017A020216022)the Industrial Development Fund Support Project of Dapeng District,Shenzhen,China(KY20180117)。
文摘Mikania micrantha Kunth is an invasive alien weed and known as a plant killer around the world.Accurately and rapidly identifying M.micrantha in the wild is important for monitoring its growth status,as this helps management officials to take the necessary steps to devise a comprehensive strategy to control the invasive weed in the identified area.However,this approach still mainly depends on satellite remote sensing and manual inspection.The cost is high and the accuracy rate and efficiency are low.We acquired color images of the monitoring area in the wild environment using an Unmanned Aerial Vehicle(UAV)and proposed a novel network-MmNet-based on a deep Convolutional Neural Network(CNN)to identify M.micrantha in the images.The network consists of AlexNet Local Response Normalization(LRN),along with the GoogLeNet and continuous convolution of VGG inception models.After training and testing,the identification of 400 testing samples by MmNet is very good,with accuracy of 94.50%and time cost of 10.369 s.Moreover,in quantitative comparative analysis,the proposed MmNet not only has high accuracy and efficiency but also simple construction and outstanding repeatability.Compared with recently popular CNNs,MmNet is more suitable for the identification of M.micrantha in the wild.However,to meet the challenge of wild environments,more M.micrantha images need to be acquired for MmNet training.In addition,the classification labels need to be sorted in more detail.Altogether,this research provides some theoretical and scientific basis for the development of intelligent monitoring and early warning systems for M.micrantha and other invasive species.
基金funded by Directorate of Research and Humanity Engagement (DRPM), Universitas Indonesia via grant “Hibah PITTA 2017” with No. 328/UN2.R3.1/HKP.05.00/ 2017
文摘Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concen-tration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida:Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X12 – 3.210 X32 – 6.786 X42 – 7.290 X12X3 + 5.575 X1X32 – 4.843 X32X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.1725μg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.
基金supported by grant “Hibah Tugas Akhir Mahasiswa Doktor(TADOK)Tahun 2018” Directorate of Research and Humanity Engagement Universitas Indonesia(grant number:1234/UN2.R3.1/HKP.05.00/2018)
文摘Objective:To isolate,identify,and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida(L.)Kunth herbs.Methods:A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate.The ethyl acetate extract solution was evaporated to obtain the crude extract.Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds.Then,both compounds were elucidated and identified using the spectroscopic method.Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength.Results:Two bioactive compounds were successfully isolated from Peperomia pellucida herb,including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1 H-indene and pellucidin A.Both compounds demonstrated angiotensin-converting enzyme inhibitory activity,with IC50 values of 72 μM(27.95 μg/mL)and 1 1μM(4.4 μg/mL),respectively.Conclusions:In the present study,two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine,and support its use as an angiotensin-converting enzyme-inhibiting drug.
文摘Piper auritum Kunth,a member of the Piperacea family,is a small shrub native to the Central American tropics.This plant was traditionally used in Latin American countries for the treatment of various medical conditions.Increase in the recent studies of P.auritum’s chemical components leads to the isolation of monoterpenoids,sesquiterpenoids,phenylpropanoids and several other compounds.Previous research has revealed that these secondary metabolites have diverse pharmacological activities including antidiabetic,antioxidant,anti-inflammatory,anti-pathogeny microorganism and repellent activity.This review summarizes relevant findings on P.auritum,and hopes to contribute to the exploration of its potentials for further development as an alternative medicine.
文摘以苗族常用药材吉祥草(Reineckia carnea(Andr.)Kunth)为试材,采用盆栽控制试验,设置了对照(CK)、单独增二氧化碳(eC:CO_(2)浓度为(800±20)μmol·mol^(-1))、单独施氮(eN:施加5 g NH_(4)NO_(3))、同时增二氧化碳与施氮(eC+eN:CO_(2)浓度为(800±20)μmol·mol^(-1),且施加5 g NH_(4)NO_(3))4个处理,研究CO_(2)浓度升高、氮沉降及二者的交互作用对吉祥草的生长及其药用成分的影响,以期为吉祥草在药用生产上提供参考依据。结果表明:1)升高CO_(2)浓度和施氮或同时升高CO_(2)浓度和施氮均显著促进了吉祥草的形态生长,总生物量和可溶性蛋白质含量显著增加,茎中的碳含量显著降低,超氧化物歧化酶(SOD)活性无显著变化;2)施氮处理下全氮含量显著增加,过氧化氢酶(CAT)活性、根和叶中的皂苷含量显著下降,在此基础上升高CO_(2)浓度可缓解皂苷下降,CAT活性显著升高,内源激素含量在施氮或同时升高CO_(2)浓度和施氮处理下显著增加。综上所述,在实际生产过程中,可适当增加CO_(2)浓度,氮肥浓度不宜过高,有利于吉祥草的生长,并保证其药用品质不受损害。
