Exosomes exhibit complex biological functions and mediate a variety of biological processes,such as promoting axonal regeneration and functional recove ry after injury.Long non-coding RNAs(IncRNAs)have been reported t...Exosomes exhibit complex biological functions and mediate a variety of biological processes,such as promoting axonal regeneration and functional recove ry after injury.Long non-coding RNAs(IncRNAs)have been reported to play a crucial role in axonal regeneration.Howeve r,the role of the IncRNA-microRNAmessenger RNA(mRNA)-competitive endogenous RNA(ceRNA)network in exosome-mediated axonal regeneration remains unclear.In this study,we performed RNA transcriptome sequencing analysis to assess mRNA expression patterns in exosomes produced by cultured fibroblasts(FC-EXOs)and Schwann cells(SCEXOs).Diffe rential gene expression analysis,Gene Ontology analysis,Kyoto Encyclopedia of Genes and Genomes analysis,and protein-protein intera ction network analysis were used to explo re the functions and related pathways of RNAs isolated from FC-EXOs and SC-EXOs.We found that the ribosome-related central gene Rps5 was enriched in FC-EXOs and SC-EXOs,which suggests that it may promote axonal regeneration.In addition,using the miRWalk and Starbase prediction databases,we constructed a regulatory network of ceRNAs targeting Rps5,including 27 microRNAs and five IncRNAs.The ceRNA regulatory network,which included Ftx and Miat,revealed that exsosome-derived Rps5 inhibits scar formation and promotes axonal regeneration and functional recovery after nerve injury.Our findings suggest that exosomes derived from fibro blast and Schwann cells could be used to treat injuries of peripheral nervous system.展开更多
Conservation tillage as an effective alternative to mitigate soil degradation has attracted worldwide attention,but the influences of conservation tillage on soil microbial community and especially function remain unc...Conservation tillage as an effective alternative to mitigate soil degradation has attracted worldwide attention,but the influences of conservation tillage on soil microbial community and especially function remain unclear.Shotgun metagenomics sequencing was performed to examine the taxonomic and functional community variations of black soils under three tillage regimes,namely no-tillage with residue(maize straw)return(NTS),moldboard plow with residue return(MPS),and moldboard plow without residue return(MPN)in Northeast China.The results revealed:1)Soil bacterial and archaeal communities differed significantly under different tillage regimes in contrast to soil fungal community.2)The overlay of less tillage and residues return under NTS led to unique soil microbial community composition and functional composition.Specifically,in contrast to other treatments,NTS increased the relative abundances of some taxa such as Bradyrhizobium,Candidatus Solibacter,and Reyranella,along with the relative abundances of some taxa such as Sphingomonas,Unclassified Chloroflexi and Nitrososphaera decreased;NTS had a unique advantage of increasing the relative abundances of genes involved in‘ATP-binding cassette(ABC)transporters’and‘quorum sensing(QS)’pathways,while MPN favored the genes involved in‘flagellar assembly’pathway and some metabolic pathways such as‘carbon’and‘glyoxylate and dicarboxylate’and‘selenocompound’metabolisms.3)Significantly different soil bacterial phyla(Acidobacteria,Gemmatimonadetes,and Chloroflexi)and metabolic pathways existed between MPN and another two treatments(NTS and MPS),while did not exist between NTS and MPS.4)Dissolved organic carbon(DOC)and soil bulk density were significantly affected(P<0.05)by tillage and accounted for the variance both in microbial(bacterial)community structure and functional composition.These results indicated that a change in tillage regime from conventional to conservation tillage results in a shift of microbial community and functional genes,and we inferred that residue return played a more prominent role than less tillage in functional shifts in the microbial community of black soils.展开更多
Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety ofliver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profile...Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety ofliver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles ofaflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered whenexposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After thefunctional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such asTGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, ErbB signalingpathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated withfocal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a goodconcordance with these pathways. The quantitative real-time polymerase chain reaction（PCR） analysis of selectedgenes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecularmechanisms of aflatoxins.展开更多
Breast cancer is the leading cause of death in women. Prognosis of breast cancer is often pessimistic because the tumors are prone to metastasizing to the bone, brain, and lung. The estrogen signaling receptor (ESR) p...Breast cancer is the leading cause of death in women. Prognosis of breast cancer is often pessimistic because the tumors are prone to metastasizing to the bone, brain, and lung. The estrogen signaling receptor (ESR) pathway contains 39 main genes and proteins which makes it one of the larger signaling pathways. Predominately this pathway and the proteins within are involved in breast growth and development, making it a prospective area of study for breast cancer. While the healthy ESR pathway has been constructed and is well established, a mechanistic model of mutated genes of ESR pathway has not been delved upon. Such mutated models could be utilized for selecting combinational targets for drug therapies, as well as elucidating crosstalk between other pathways and feedback mechanisms. To construct the mutated models of the ESR pathway it is imperative to assess what is currently understood in the literature and what inconsistencies exist in order to resolve them. Without this information, a model of the ESR pathway will be unreliable and likely unproductive. This review is the detailed literature survey of the biological studies performed on ESR pathways genes, and their respective roles in breast cancer. Furthermore, the details mentioned in the review can be beneficial for the integrated study of the ESR pathway genes, which includes, structural and dynamics study of the genes products, to have a holistic understanding of the cancer mechanism.展开更多
Microorganisms plays an important role in the growth of Pyropia haitanensis.To understand the structural and functional diversity of the microorganism community of P.haitanensis(PH40),the associated metabolic pathway ...Microorganisms plays an important role in the growth of Pyropia haitanensis.To understand the structural and functional diversity of the microorganism community of P.haitanensis(PH40),the associated metabolic pathway network in cluster of orthologous groups(COG)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and carbohydrate-active enzymes(CAZymes)were explored in metagenomic analysis.DNA extraction from gametophytes of P.haitanensis was performed first,followed by library construction,sequencing,preprocessing of sequencing data,taxonomy assignment,gene prediction,and functional annotation.The results show that the predominant microorganisms of P.haitanensis were bacteria(98.98%),and the phylum with the highest abundance was Proteobacteria(54.64%),followed by Bacteroidetes(37.92%).Erythrobacter(3.98%)and Hyunsoonleella jejuensis(1.56%)were the genera and species with the highest abundance of bacteria,respectively.The COG annotation demonstrated that genes associated with microbial metabolism was the predominant category.The results of metabolic pathway annotation show that the ABC transport system and two-component system were the main pathways in the microbial community.Plant growth hormone biosynthesis pathway and multi-vitamin biosynthesis functional units(modules)were the other important pathways.The CAZyme annotation revealed that the starch might be an important carbon source for microorganisms.Glycosyl transferase family 2(GT2)and glycosyl transferase family 3(GT3)were the highly abundant families in glucoside transferase superfamily.Six metagenome-assembled genomes containing enzymes involved in the biosynthesis of cobalamin(vitamin B 12)and indole-3-acetic acid were obtained by binning method.They were confirmed to belong to Rhodobacterales and Rhizobiales,respectively.Our findings provide comprehensive insights into the microorganism community of Pyropia.展开更多
MicroRNAs （miRNAs） are a class of small RNA molecules that are implicated in post-transcriptional reg- ulation of gene expression during development. The discovery and understanding of miRNAs has revolutionized the ...MicroRNAs （miRNAs） are a class of small RNA molecules that are implicated in post-transcriptional reg- ulation of gene expression during development. The discovery and understanding of miRNAs has revolutionized the traditional view of gene expression. Alport syndrome （AS） is an inherited disorder of type IV collagen, which most commonly leads to glomerulonephritis and kidney failure. Patients with AS inevitably reach end-stage renal disease and require renal replacement therapy, starting in young adulthood. In this study, Solexa sequencing was used to identify and quantitatively profile small RNAs from an AS family. We identified 30 known miRNAs that showed a sig- nificant change in expression between two individuals. Nineteen miRNAs were up-regulated and eleven were down-regulated. Forty-nine novel miRNAs showed significantly different levels of expression between two individuals. Gene target predictions for the miRNAs revealed that high ranking target genes were implicated in cell, cell part and cellular process categories. The purine metabolism pathway and mitogen-activated protein kinase （MAPK） signaling pathway were enriched by the largest number of target genes. These results strengthen the notion that miRNAs and their target genes are involved in AS and the data advance our understanding of miRNA function in the patho- genesis of AS.展开更多
通过中药系统药理学数据库与分析平台(traditional Chinese medicine system pharmacology datebase and analysis platform,TCMSP)筛选五苓散组分白术、茯苓、猪苓、泽泻、桂枝的潜在活性成分,PharmMapper服务器预测潜在靶点,构建化合...通过中药系统药理学数据库与分析平台(traditional Chinese medicine system pharmacology datebase and analysis platform,TCMSP)筛选五苓散组分白术、茯苓、猪苓、泽泻、桂枝的潜在活性成分,PharmMapper服务器预测潜在靶点,构建化合物-靶点网络、蛋白互作(protein-protein interaction,PPI)网络,进行基因本体(gene ontology,GO)富集分析、基于KEGG(Kyoto encyclopedia of genes and enomes)的生物通路富集分析。结果表明,筛选得到五苓散中50种活性成分,构建化合物-靶点网络共459个节点,关键靶点包括CSDE1、PRPS1、HSD17B4、CLPP、ACBD7、AZGP1等。构建五苓散靶点的PPI互作网络包含155个节点和527条相互作用关系,关键节点包括CSDE1、CITED2、RANBP2、HSD17B4、TAF13、SOD2等。GO富集分析途径相关条目主要涉及细胞溶质、细胞对有机物质和化学刺激的反应,含氮物质代谢,有机物循环代谢进程,蛋白、小分子和离子结合及转运活性等。KEGG代谢通路分析包括膀胱癌、前列腺癌、内分泌抵抗、癌症中的蛋白聚糖、流体剪切应力和动脉粥样硬化等途径。研究结果初步验证了五苓散药理作用的分子机制,为进一步深入揭示其分子作用机制奠定了基础,预测五苓散的潜在作用靶点如PRPS1、HSD17B4等,可为五苓散的深入研究提供新思路。展开更多
目的应用网络药理学方法探究川芎-当归药对的主要活性成分、靶点和药理作用机制。方法研究时间为2020年9—12月。首先以“川芎”“当归”为关键词,在TCMSP 2.3数据库中检索药材的成分、靶点和对应疾病数据,构建“药物-成分靶点-疾病”网...目的应用网络药理学方法探究川芎-当归药对的主要活性成分、靶点和药理作用机制。方法研究时间为2020年9—12月。首先以“川芎”“当归”为关键词,在TCMSP 2.3数据库中检索药材的成分、靶点和对应疾病数据,构建“药物-成分靶点-疾病”网络,进行基因本体(gene ontology,GO)分类富集分析、京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,探究“川芎-当归”药对作用机制。结果“药物-成分-靶点-疾病”网络包含2个药物、10个活性成分,71个作用靶点,191种疾病。关键靶点涉及环氧化酶2(PTGS2)、过氧化物酶体增生激活受体γ(PPARG)、雌激素受体(ESR1)、β2肾上腺素能受体(ADRB2)、周期蛋白依赖激酶2(CDK2)、热休克蛋白90(HSP90)、促分裂原活化蛋白激酶14(MAPK14)、胆碱能受体2(CHRM2)、5羟色胺受体2A(HTR2A)、凝血因子Ⅱ受体(F2R)、盐皮质激素受体(NR3C2)、内皮型一氧化氮合酶(NOS3)等,关键疾病涉及疼痛、心血管疾病、乳腺癌、阿尔茨海默病、炎症、癌症、焦虑症、精神分裂症、前列腺癌、实体肿瘤、脑损伤等。GO富集分析得到237个条目,包括生物过程178个,分子功能26个,细胞组成33个。通路富集分析包含66条通路,主要涉及神经活性配体-受体相互作用、钙离子信号通道、癌症通路、5-羟色胺能突触、大肠癌、雌激素信号通路、心肌细胞的肾上腺素能信号、甲状腺激素信号通路、cAMP信号通路、血管内皮生长因子信号通路等。结论“川芎-当归”药对中多个成分作用于多个靶点和通路,对疼痛、心血管疾病、乳腺癌、阿尔茨海默病、炎症、癌症等多种疾病均一定的治疗作用。展开更多
通过非标记定量(label-free)蛋白质组学技术对宁夏中宁和青海格尔木两个产地的枸杞干果样品进行蛋白组学比较。结果表明两种样品共鉴别出蛋白质2477个,中宁产区样品中含蛋白质1942个(其中174个为中宁样品特有),格尔木产区样品中含蛋白质...通过非标记定量(label-free)蛋白质组学技术对宁夏中宁和青海格尔木两个产地的枸杞干果样品进行蛋白组学比较。结果表明两种样品共鉴别出蛋白质2477个,中宁产区样品中含蛋白质1942个(其中174个为中宁样品特有),格尔木产区样品中含蛋白质2065个(其中297个为格尔木样品特有);另外中宁产地的样品和格尔木产地的样品相比有86个蛋白质的表达量下调,69个蛋白质的表达量上调。对样品蛋白质进行层次聚类分析,证明两产地样品在蛋白质组学上有明显差异。利用GO数据库和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)数据库对所有差异蛋白质进行检索,筛选后得到28个目标差异蛋白质,其中包括:4个金属离子转运蛋白、6个热休克蛋白、3个几丁质酶、4个磷酸肌醇转化酶、2个过氧化氢酶、3个与植物激素调节有关的蛋白质、5个与卟啉和叶绿素代谢相关的酶、1个核糖核苷二磷酸激酶。展开更多
基金supported by the National Natural Science Foundation of China,No.81870975(to SZ)。
文摘Exosomes exhibit complex biological functions and mediate a variety of biological processes,such as promoting axonal regeneration and functional recove ry after injury.Long non-coding RNAs(IncRNAs)have been reported to play a crucial role in axonal regeneration.Howeve r,the role of the IncRNA-microRNAmessenger RNA(mRNA)-competitive endogenous RNA(ceRNA)network in exosome-mediated axonal regeneration remains unclear.In this study,we performed RNA transcriptome sequencing analysis to assess mRNA expression patterns in exosomes produced by cultured fibroblasts(FC-EXOs)and Schwann cells(SCEXOs).Diffe rential gene expression analysis,Gene Ontology analysis,Kyoto Encyclopedia of Genes and Genomes analysis,and protein-protein intera ction network analysis were used to explo re the functions and related pathways of RNAs isolated from FC-EXOs and SC-EXOs.We found that the ribosome-related central gene Rps5 was enriched in FC-EXOs and SC-EXOs,which suggests that it may promote axonal regeneration.In addition,using the miRWalk and Starbase prediction databases,we constructed a regulatory network of ceRNAs targeting Rps5,including 27 microRNAs and five IncRNAs.The ceRNA regulatory network,which included Ftx and Miat,revealed that exsosome-derived Rps5 inhibits scar formation and promotes axonal regeneration and functional recovery after nerve injury.Our findings suggest that exosomes derived from fibro blast and Schwann cells could be used to treat injuries of peripheral nervous system.
基金Under the auspices of the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA2307050103)National Natural Science Foundation of China(No.42071064,41877095)the Project of Changchun Science and Technology Plan(No.19SS019)。
文摘Conservation tillage as an effective alternative to mitigate soil degradation has attracted worldwide attention,but the influences of conservation tillage on soil microbial community and especially function remain unclear.Shotgun metagenomics sequencing was performed to examine the taxonomic and functional community variations of black soils under three tillage regimes,namely no-tillage with residue(maize straw)return(NTS),moldboard plow with residue return(MPS),and moldboard plow without residue return(MPN)in Northeast China.The results revealed:1)Soil bacterial and archaeal communities differed significantly under different tillage regimes in contrast to soil fungal community.2)The overlay of less tillage and residues return under NTS led to unique soil microbial community composition and functional composition.Specifically,in contrast to other treatments,NTS increased the relative abundances of some taxa such as Bradyrhizobium,Candidatus Solibacter,and Reyranella,along with the relative abundances of some taxa such as Sphingomonas,Unclassified Chloroflexi and Nitrososphaera decreased;NTS had a unique advantage of increasing the relative abundances of genes involved in‘ATP-binding cassette(ABC)transporters’and‘quorum sensing(QS)’pathways,while MPN favored the genes involved in‘flagellar assembly’pathway and some metabolic pathways such as‘carbon’and‘glyoxylate and dicarboxylate’and‘selenocompound’metabolisms.3)Significantly different soil bacterial phyla(Acidobacteria,Gemmatimonadetes,and Chloroflexi)and metabolic pathways existed between MPN and another two treatments(NTS and MPS),while did not exist between NTS and MPS.4)Dissolved organic carbon(DOC)and soil bulk density were significantly affected(P<0.05)by tillage and accounted for the variance both in microbial(bacterial)community structure and functional composition.These results indicated that a change in tillage regime from conventional to conservation tillage results in a shift of microbial community and functional genes,and we inferred that residue return played a more prominent role than less tillage in functional shifts in the microbial community of black soils.
文摘Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety ofliver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles ofaflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered whenexposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After thefunctional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such asTGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, ErbB signalingpathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated withfocal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a goodconcordance with these pathways. The quantitative real-time polymerase chain reaction（PCR） analysis of selectedgenes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecularmechanisms of aflatoxins.
文摘Breast cancer is the leading cause of death in women. Prognosis of breast cancer is often pessimistic because the tumors are prone to metastasizing to the bone, brain, and lung. The estrogen signaling receptor (ESR) pathway contains 39 main genes and proteins which makes it one of the larger signaling pathways. Predominately this pathway and the proteins within are involved in breast growth and development, making it a prospective area of study for breast cancer. While the healthy ESR pathway has been constructed and is well established, a mechanistic model of mutated genes of ESR pathway has not been delved upon. Such mutated models could be utilized for selecting combinational targets for drug therapies, as well as elucidating crosstalk between other pathways and feedback mechanisms. To construct the mutated models of the ESR pathway it is imperative to assess what is currently understood in the literature and what inconsistencies exist in order to resolve them. Without this information, a model of the ESR pathway will be unreliable and likely unproductive. This review is the detailed literature survey of the biological studies performed on ESR pathways genes, and their respective roles in breast cancer. Furthermore, the details mentioned in the review can be beneficial for the integrated study of the ESR pathway genes, which includes, structural and dynamics study of the genes products, to have a holistic understanding of the cancer mechanism.
基金Supported by the National Key R&D Program of China(Nos.2018YFC1406704,2018YFD0900106,2018YFC1406700)the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0302-4)the MOA Modern Agricultural Talents Support Project。
文摘Microorganisms plays an important role in the growth of Pyropia haitanensis.To understand the structural and functional diversity of the microorganism community of P.haitanensis(PH40),the associated metabolic pathway network in cluster of orthologous groups(COG)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and carbohydrate-active enzymes(CAZymes)were explored in metagenomic analysis.DNA extraction from gametophytes of P.haitanensis was performed first,followed by library construction,sequencing,preprocessing of sequencing data,taxonomy assignment,gene prediction,and functional annotation.The results show that the predominant microorganisms of P.haitanensis were bacteria(98.98%),and the phylum with the highest abundance was Proteobacteria(54.64%),followed by Bacteroidetes(37.92%).Erythrobacter(3.98%)and Hyunsoonleella jejuensis(1.56%)were the genera and species with the highest abundance of bacteria,respectively.The COG annotation demonstrated that genes associated with microbial metabolism was the predominant category.The results of metabolic pathway annotation show that the ABC transport system and two-component system were the main pathways in the microbial community.Plant growth hormone biosynthesis pathway and multi-vitamin biosynthesis functional units(modules)were the other important pathways.The CAZyme annotation revealed that the starch might be an important carbon source for microorganisms.Glycosyl transferase family 2(GT2)and glycosyl transferase family 3(GT3)were the highly abundant families in glucoside transferase superfamily.Six metagenome-assembled genomes containing enzymes involved in the biosynthesis of cobalamin(vitamin B 12)and indole-3-acetic acid were obtained by binning method.They were confirmed to belong to Rhodobacterales and Rhizobiales,respectively.Our findings provide comprehensive insights into the microorganism community of Pyropia.
基金supported by the Shenzhen Knowledge Innovation Program of Basic Research Items of Guangdong Province(No.JCYJ2014 0416122812045),China
文摘MicroRNAs （miRNAs） are a class of small RNA molecules that are implicated in post-transcriptional reg- ulation of gene expression during development. The discovery and understanding of miRNAs has revolutionized the traditional view of gene expression. Alport syndrome （AS） is an inherited disorder of type IV collagen, which most commonly leads to glomerulonephritis and kidney failure. Patients with AS inevitably reach end-stage renal disease and require renal replacement therapy, starting in young adulthood. In this study, Solexa sequencing was used to identify and quantitatively profile small RNAs from an AS family. We identified 30 known miRNAs that showed a sig- nificant change in expression between two individuals. Nineteen miRNAs were up-regulated and eleven were down-regulated. Forty-nine novel miRNAs showed significantly different levels of expression between two individuals. Gene target predictions for the miRNAs revealed that high ranking target genes were implicated in cell, cell part and cellular process categories. The purine metabolism pathway and mitogen-activated protein kinase （MAPK） signaling pathway were enriched by the largest number of target genes. These results strengthen the notion that miRNAs and their target genes are involved in AS and the data advance our understanding of miRNA function in the patho- genesis of AS.
文摘通过中药系统药理学数据库与分析平台(traditional Chinese medicine system pharmacology datebase and analysis platform,TCMSP)筛选五苓散组分白术、茯苓、猪苓、泽泻、桂枝的潜在活性成分,PharmMapper服务器预测潜在靶点,构建化合物-靶点网络、蛋白互作(protein-protein interaction,PPI)网络,进行基因本体(gene ontology,GO)富集分析、基于KEGG(Kyoto encyclopedia of genes and enomes)的生物通路富集分析。结果表明,筛选得到五苓散中50种活性成分,构建化合物-靶点网络共459个节点,关键靶点包括CSDE1、PRPS1、HSD17B4、CLPP、ACBD7、AZGP1等。构建五苓散靶点的PPI互作网络包含155个节点和527条相互作用关系,关键节点包括CSDE1、CITED2、RANBP2、HSD17B4、TAF13、SOD2等。GO富集分析途径相关条目主要涉及细胞溶质、细胞对有机物质和化学刺激的反应,含氮物质代谢,有机物循环代谢进程,蛋白、小分子和离子结合及转运活性等。KEGG代谢通路分析包括膀胱癌、前列腺癌、内分泌抵抗、癌症中的蛋白聚糖、流体剪切应力和动脉粥样硬化等途径。研究结果初步验证了五苓散药理作用的分子机制,为进一步深入揭示其分子作用机制奠定了基础,预测五苓散的潜在作用靶点如PRPS1、HSD17B4等,可为五苓散的深入研究提供新思路。
文摘目的应用网络药理学方法探究川芎-当归药对的主要活性成分、靶点和药理作用机制。方法研究时间为2020年9—12月。首先以“川芎”“当归”为关键词,在TCMSP 2.3数据库中检索药材的成分、靶点和对应疾病数据,构建“药物-成分靶点-疾病”网络,进行基因本体(gene ontology,GO)分类富集分析、京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,探究“川芎-当归”药对作用机制。结果“药物-成分-靶点-疾病”网络包含2个药物、10个活性成分,71个作用靶点,191种疾病。关键靶点涉及环氧化酶2(PTGS2)、过氧化物酶体增生激活受体γ(PPARG)、雌激素受体(ESR1)、β2肾上腺素能受体(ADRB2)、周期蛋白依赖激酶2(CDK2)、热休克蛋白90(HSP90)、促分裂原活化蛋白激酶14(MAPK14)、胆碱能受体2(CHRM2)、5羟色胺受体2A(HTR2A)、凝血因子Ⅱ受体(F2R)、盐皮质激素受体(NR3C2)、内皮型一氧化氮合酶(NOS3)等,关键疾病涉及疼痛、心血管疾病、乳腺癌、阿尔茨海默病、炎症、癌症、焦虑症、精神分裂症、前列腺癌、实体肿瘤、脑损伤等。GO富集分析得到237个条目,包括生物过程178个,分子功能26个,细胞组成33个。通路富集分析包含66条通路,主要涉及神经活性配体-受体相互作用、钙离子信号通道、癌症通路、5-羟色胺能突触、大肠癌、雌激素信号通路、心肌细胞的肾上腺素能信号、甲状腺激素信号通路、cAMP信号通路、血管内皮生长因子信号通路等。结论“川芎-当归”药对中多个成分作用于多个靶点和通路,对疼痛、心血管疾病、乳腺癌、阿尔茨海默病、炎症、癌症等多种疾病均一定的治疗作用。
文摘通过非标记定量(label-free)蛋白质组学技术对宁夏中宁和青海格尔木两个产地的枸杞干果样品进行蛋白组学比较。结果表明两种样品共鉴别出蛋白质2477个,中宁产区样品中含蛋白质1942个(其中174个为中宁样品特有),格尔木产区样品中含蛋白质2065个(其中297个为格尔木样品特有);另外中宁产地的样品和格尔木产地的样品相比有86个蛋白质的表达量下调,69个蛋白质的表达量上调。对样品蛋白质进行层次聚类分析,证明两产地样品在蛋白质组学上有明显差异。利用GO数据库和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)数据库对所有差异蛋白质进行检索,筛选后得到28个目标差异蛋白质,其中包括:4个金属离子转运蛋白、6个热休克蛋白、3个几丁质酶、4个磷酸肌醇转化酶、2个过氧化氢酶、3个与植物激素调节有关的蛋白质、5个与卟啉和叶绿素代谢相关的酶、1个核糖核苷二磷酸激酶。
