The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 eja...The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk (group Ⅰ), DHA-enriched hen egg yolk (group Ⅱ), normal hen egg yolk with 5 mmol L^-1 of cysteine supplementation (group Ⅲ) and DHA-enriched hen egg yolk with 5 mmol L1 of cysteine supplementation (group Ⅳ). The semen was cryopreserved using controlled rate freezer and was thawed at 50℃ for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone (group Ⅲ) improved progressive motility (P 〈 0.05), and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk (group Ⅳ) improved both progressive motility (P 〈 0.05) and acrosome integrity (P 〈 0.01). The use of DHA-enriched hen egg yolk alone (group Ⅱ) did not enhance any of the post-thawed semen qualities (P 〉 0.05). In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.展开更多
Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. ...Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L^-1 (group Ⅰ, control), 5 mmol L^-1 (group Ⅱ), 10 mmol L^-1 (group Ⅲ) and 15 mmol L^-1 (group Ⅳ). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P 〈 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group Ⅱ and group Ⅲ) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group Ⅲ. In conclusion, 5 or 10 mmol L^-1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.展开更多
Docetaxel-based combination chemotherapy remains the predominant treatment for castration-resistant prostate cancer. However, taxane-related drug resistance and neurotoxicity have prompted us to develop substitute tre...Docetaxel-based combination chemotherapy remains the predominant treatment for castration-resistant prostate cancer. However, taxane-related drug resistance and neurotoxicity have prompted us to develop substitute treatment strategies. Eg5 (kinesin spindle protein), which is crucial for bipolar spindle formation and duplicated chromosome separation during the early phase of mitosis, has emerged as an attractive target for cancer chemotherapy. The aim of this study was to investigate the anticancer efficacy of $-(methoxytrityl)-L-cysteine (S(MeO)TLC), a novel Eg5 inhibitor in prostate cancer. Eg5 expression was examined in human prostate cancer cell lines and tissue microarrays were constructed from clinical specimens. Antiproliferative activity of S(MeO)TLC in prostate cancer cells was assessed by a cell viability assay. The anticancer effect and inhibitory mechanism of S(MeO)TLC in prostate cancer cells was further explored by Hoechst staining, flow cytometry and immunofluorescence. In addition, the antitumor effect of S(MeO)TLC on subcutaneous xenograft models was assessed. Eg5 expression was identified in PC3, DU145 and LNCaP cells. More than half of prostate cancer clinical specimens displayed Eg5 expression. S(Me0)TLC exhibited more powerful anticancer activity in prostate cancer cells compared with the other four Eg5 inhibitors tested. S(MeO)TLC induced cell death after arresting dividing cells at mitosis with distinct monopolar spindle formation. S(MeO)TLC exhibited its significant inhibitory activity (P〈0.05) on subcutaneous xenograft models also through induction of mitotic arrest. We conclude that Eg5 is a good target for prostate cancer chemotherapy, and S(MeO)TLC is a potent promising anticancer agent in prostate cancer.展开更多
The film contained L-cysteine and gold nanoparticles were provided by self-assembled monolayers (SAMs) and potentiostatic electrodeposition technology on the gold electrode. Two methods were used to study the film: In...The film contained L-cysteine and gold nanoparticles were provided by self-assembled monolayers (SAMs) and potentiostatic electrodeposition technology on the gold electrode. Two methods were used to study the film: In the first, cyclic voltammetry (CV) was used to inspect the functional groups of the film and the same time hydroquinone was chosen to be a probe molecule in the based solution;secondly, based on analytical technology of scanning electrochemical microscopy (SECM), the heterogeneous rate constant (keff) between solid phase (the modified electrode) and liquid phase (K3Fe(CN)6) was obtained. As a result, the better binary catalysis of hydroquinone was demonstrated and the heterogeneous rate constant (keff) is the greater at 8 h for L-cysteine self-assembled monolayers (SAMs).展开更多
An effective L-cysteine-assisted synthetic route has been successfully developed to prepare copper gallium sulfide(CuGaS2) microspheres under solvothermal conditions with CuCI2.2HzO, GaC13 and L-cysteine as source m...An effective L-cysteine-assisted synthetic route has been successfully developed to prepare copper gallium sulfide(CuGaS2) microspheres under solvothermal conditions with CuCI2.2HzO, GaC13 and L-cysteine as source materials, in which L-cysteine was used as the sulfide source and complexing molecule. The experiments revealed that the synthesized sample was of a typical CuGaS2 tetragonal structure. Moreover, the prepared CuGaS2 crystals consisting of microspheres made up of nanoflakes, and the diameter of the nanoflakes was about 20 nm. Raman spectrum of the obtained CuGaS2 exhibits a high-intensity peak of the A1 mode at 306 cm^-1. Meanwhile, a possible growth mechanism was proposed based on the investigations.展开更多
<span style="font-family:Verdana;"><i><span style="font-family:Verdana;"><i></span></i></span><span style="font-family:Verdana;"><span s...<span style="font-family:Verdana;"><i><span style="font-family:Verdana;"><i></span></i></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">S</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">-allyl-L-cysteine (SAC) is an organosulfur compound derived from aged garlic extract (</span><span style="font-family:Verdana;">AGE</span><span style="font-family:Verdana;">). Studies have reported that AGE possesses bioprotective capacity, including antidiabetic, antimicrobial, antioxidant, and antitumor effects. The present study examined the protective effects of SAC against carbon tetrachloride (CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">) induced hepatotoxicity in rats. Ten male Wistar rats aged 11 - 12 weeks were randomly divided into two groups (five rats/group) as control and SAC groups. All rats had </span><span style="font-family:Verdana;">ad</span> <span style="font-family:Verdana;">libitum</span><span style="font-family:Verdana;"> access to water, and the SAC group received water containing SAC intragastrically (200 mg/kg) once daily for five consecutive weeks. In the fifth experimental week, 50% CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> in olive oil (1 mL/kg) was administered intraperitoneally three times a week to induce liver injury in both groups. Rats were sacrificed at 24 hours after the last CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> injection, and liver tissues were excised for histopathological, immunohistochemical and antioxidant analyses. The rats in the SAC group did not show abnormal behavior, such as decreased water intake or food consumption, during the experimental period. Body weights in all groups did not change significantly over the experimental period. Histopathological analysis showed that the percentage of hepatic steatosis was lower in the SAC group at 12.75% ± 3.74% compared to 24.64% ± 5.29% in the control group (</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> < 0.05). The percentage of cytochrome P4502E1 (CYP2E1) distribution area in the SAC group was also lower at 19.61% ± 6.18% compared with 25.22% ± 6.21% in the control group (</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> < 0.05). These results suggest that SAC can alleviate CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">-induced liver damage by decreasing hepatic steatosis and reducing CYP2E1 expression in rats.展开更多
文摘The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk (group Ⅰ), DHA-enriched hen egg yolk (group Ⅱ), normal hen egg yolk with 5 mmol L^-1 of cysteine supplementation (group Ⅲ) and DHA-enriched hen egg yolk with 5 mmol L1 of cysteine supplementation (group Ⅳ). The semen was cryopreserved using controlled rate freezer and was thawed at 50℃ for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone (group Ⅲ) improved progressive motility (P 〈 0.05), and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk (group Ⅳ) improved both progressive motility (P 〈 0.05) and acrosome integrity (P 〈 0.01). The use of DHA-enriched hen egg yolk alone (group Ⅱ) did not enhance any of the post-thawed semen qualities (P 〉 0.05). In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.
文摘Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L^-1 (group Ⅰ, control), 5 mmol L^-1 (group Ⅱ), 10 mmol L^-1 (group Ⅲ) and 15 mmol L^-1 (group Ⅳ). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P 〈 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group Ⅱ and group Ⅲ) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group Ⅲ. In conclusion, 5 or 10 mmol L^-1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.
文摘Docetaxel-based combination chemotherapy remains the predominant treatment for castration-resistant prostate cancer. However, taxane-related drug resistance and neurotoxicity have prompted us to develop substitute treatment strategies. Eg5 (kinesin spindle protein), which is crucial for bipolar spindle formation and duplicated chromosome separation during the early phase of mitosis, has emerged as an attractive target for cancer chemotherapy. The aim of this study was to investigate the anticancer efficacy of $-(methoxytrityl)-L-cysteine (S(MeO)TLC), a novel Eg5 inhibitor in prostate cancer. Eg5 expression was examined in human prostate cancer cell lines and tissue microarrays were constructed from clinical specimens. Antiproliferative activity of S(MeO)TLC in prostate cancer cells was assessed by a cell viability assay. The anticancer effect and inhibitory mechanism of S(MeO)TLC in prostate cancer cells was further explored by Hoechst staining, flow cytometry and immunofluorescence. In addition, the antitumor effect of S(MeO)TLC on subcutaneous xenograft models was assessed. Eg5 expression was identified in PC3, DU145 and LNCaP cells. More than half of prostate cancer clinical specimens displayed Eg5 expression. S(Me0)TLC exhibited more powerful anticancer activity in prostate cancer cells compared with the other four Eg5 inhibitors tested. S(MeO)TLC induced cell death after arresting dividing cells at mitosis with distinct monopolar spindle formation. S(MeO)TLC exhibited its significant inhibitory activity (P〈0.05) on subcutaneous xenograft models also through induction of mitotic arrest. We conclude that Eg5 is a good target for prostate cancer chemotherapy, and S(MeO)TLC is a potent promising anticancer agent in prostate cancer.
文摘The film contained L-cysteine and gold nanoparticles were provided by self-assembled monolayers (SAMs) and potentiostatic electrodeposition technology on the gold electrode. Two methods were used to study the film: In the first, cyclic voltammetry (CV) was used to inspect the functional groups of the film and the same time hydroquinone was chosen to be a probe molecule in the based solution;secondly, based on analytical technology of scanning electrochemical microscopy (SECM), the heterogeneous rate constant (keff) between solid phase (the modified electrode) and liquid phase (K3Fe(CN)6) was obtained. As a result, the better binary catalysis of hydroquinone was demonstrated and the heterogeneous rate constant (keff) is the greater at 8 h for L-cysteine self-assembled monolayers (SAMs).
基金Supported by the National Natural Science Foundation of China(Nos.507872075 and 50972107)the Project of Key Scientific and Technological Innovations Team of Zhejiang Province,China(No.2009R50010)the Key Foundation of Natural Science Foundation of Zhejiang Province,China(No.Z4110347)
文摘An effective L-cysteine-assisted synthetic route has been successfully developed to prepare copper gallium sulfide(CuGaS2) microspheres under solvothermal conditions with CuCI2.2HzO, GaC13 and L-cysteine as source materials, in which L-cysteine was used as the sulfide source and complexing molecule. The experiments revealed that the synthesized sample was of a typical CuGaS2 tetragonal structure. Moreover, the prepared CuGaS2 crystals consisting of microspheres made up of nanoflakes, and the diameter of the nanoflakes was about 20 nm. Raman spectrum of the obtained CuGaS2 exhibits a high-intensity peak of the A1 mode at 306 cm^-1. Meanwhile, a possible growth mechanism was proposed based on the investigations.
文摘<span style="font-family:Verdana;"><i><span style="font-family:Verdana;"><i></span></i></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">S</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">-allyl-L-cysteine (SAC) is an organosulfur compound derived from aged garlic extract (</span><span style="font-family:Verdana;">AGE</span><span style="font-family:Verdana;">). Studies have reported that AGE possesses bioprotective capacity, including antidiabetic, antimicrobial, antioxidant, and antitumor effects. The present study examined the protective effects of SAC against carbon tetrachloride (CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">) induced hepatotoxicity in rats. Ten male Wistar rats aged 11 - 12 weeks were randomly divided into two groups (five rats/group) as control and SAC groups. All rats had </span><span style="font-family:Verdana;">ad</span> <span style="font-family:Verdana;">libitum</span><span style="font-family:Verdana;"> access to water, and the SAC group received water containing SAC intragastrically (200 mg/kg) once daily for five consecutive weeks. In the fifth experimental week, 50% CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> in olive oil (1 mL/kg) was administered intraperitoneally three times a week to induce liver injury in both groups. Rats were sacrificed at 24 hours after the last CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> injection, and liver tissues were excised for histopathological, immunohistochemical and antioxidant analyses. The rats in the SAC group did not show abnormal behavior, such as decreased water intake or food consumption, during the experimental period. Body weights in all groups did not change significantly over the experimental period. Histopathological analysis showed that the percentage of hepatic steatosis was lower in the SAC group at 12.75% ± 3.74% compared to 24.64% ± 5.29% in the control group (</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> < 0.05). The percentage of cytochrome P4502E1 (CYP2E1) distribution area in the SAC group was also lower at 19.61% ± 6.18% compared with 25.22% ± 6.21% in the control group (</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> < 0.05). These results suggest that SAC can alleviate CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">-induced liver damage by decreasing hepatic steatosis and reducing CYP2E1 expression in rats.