Faba bean(Vicia faba L.)has been identified as a rich source of L-DOPA,which is used in treating Parkinson's disease.Biosynthesis and accumulation of active substances such as L-DOPA in plant tissues may interact ...Faba bean(Vicia faba L.)has been identified as a rich source of L-DOPA,which is used in treating Parkinson's disease.Biosynthesis and accumulation of active substances such as L-DOPA in plant tissues may interact with growing conditions and processing methods.Accumulation trends of L-DOPA in various faba bean organs and the effect of drought stress and N fertilization on L-DOPA content were studied in a field and two greenhouse experiments.The influence of various processing methods on L-DOPA content of faba bean tissues was evaluated.The highest L-DOPA content was detected in fresh leaves(22.4 mg g^(-1))followed by flowers,young pods,mature seeds,and roots.Regardless of processing method,L-DOPA concentration in faba bean tissues was significantly reduced when tissues were boiled or dried.Among various methods of processing,freezing had the lowest detrimental effect,reducing L-DOPA concentrations by 24.1%and 21.1%in leaves and seeds,respectively.Drought stress elevated L-DOPA concentration,and maximum L-DOPA(23.3 mg g^(-1)of biomass)was extracted from plants grown under severe drought stress.However,L-DOPA yield(L-DOPA concentration×biomass)was compromised,owing to the adverse influence of drought stress on dry matter production.No significant difference in L-DOPA concentration was detected among various N application rates.展开更多
Fe3O4 magnetic nanoparticles were prepared by co-precipitation of Fe^2＋ and Fe^3＋ in an ammonia solution, and its size was about 36 nm measured by an atomic force microscope. Fe3O4 magnetic nanoparticles were modifi...Fe3O4 magnetic nanoparticles were prepared by co-precipitation of Fe^2＋ and Fe^3＋ in an ammonia solution, and its size was about 36 nm measured by an atomic force microscope. Fe3O4 magnetic nanoparticles were modified by L-dopa or dopamine using sonication method. The analysis of FTIR clearly indicated the formation of Fe-O-C bond. Direct immobilization of trypsin （EC： 3.4.21.4） on Fe3O4 magnetic nanoparticles with L-dopa and dopamine spacer was investigated using glutaraldehyde as a coupling agent. No significant changes in the size and magnetic property of the three kinds of magnetic nanoparticles linked with or without trypsin were observed. The existence of the spacer molecule on magnetic nanoparticles could greatly improve the activity and the storage stability of bound trypsin through increasing the flexibility of enzyme and changing the microenvironment on nanoparticles surface compared to the naked magnetic nanoparticles.展开更多
Four new chiral 1,2,3,4-tetrahydroisoquinoline-derived β-amino alcohols were synthesized from L-DOPA in good yields. The structures of the target compounds were confirmed by ^1H NMR, ^13C NMR and MS.
Parkinson’s disease was first formally identified by British physician James Parkinson in 1817 as “The Shaking Palsy”. L-DOPA (3,4-dihydroxy-phenyl-L-alanine) has been considered as a gold-standard treatment for Pa...Parkinson’s disease was first formally identified by British physician James Parkinson in 1817 as “The Shaking Palsy”. L-DOPA (3,4-dihydroxy-phenyl-L-alanine) has been considered as a gold-standard treatment for Parkinson’s disease. The world market for L-DOPA is about 250 t/year and the total market volume is about $101 billion per year. The present review summarizes the different biological sources for the production of L-DOPA. The process for L-DOPA production from different biological sources has advantages over the chemical methods such as, enantiometrically pure L-DOPA, less incubation time and cost effective method. L-DOPA is found naturally in certain plant foods, particularly broad beans which found to replenish brain levels of L-DOPA even more quickly, and for longer periods, than conventional medication.展开更多
Objective: To investigate L-3, 4-dihydroxyphenylalanine(L-dopa, anti-Parkinson drug),anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa(M. macrocarpa) beans.Me...Objective: To investigate L-3, 4-dihydroxyphenylalanine(L-dopa, anti-Parkinson drug),anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa(M. macrocarpa) beans.Methods: L-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography(RPHPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential(2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid.Results: RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of L-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong antiinflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid(43.795 mg/g), gallic acid(0.864 mg/g), p-coumaric acid(0.364 mg/g) and p-hydroxybenzoic acid(0.036 mg/g) quantified successfully from M. macrocarpa beans respectively.Conclusions: This study suggests that M. macrocarpa is a potential source of L-dopa with promising anti-inflammatory, antioxidant and nutritional benefits.展开更多
基金partially funded through a graduate student grant received from Northeast Sustainable Agriculture Research and Education(GNE14-078)
文摘Faba bean(Vicia faba L.)has been identified as a rich source of L-DOPA,which is used in treating Parkinson's disease.Biosynthesis and accumulation of active substances such as L-DOPA in plant tissues may interact with growing conditions and processing methods.Accumulation trends of L-DOPA in various faba bean organs and the effect of drought stress and N fertilization on L-DOPA content were studied in a field and two greenhouse experiments.The influence of various processing methods on L-DOPA content of faba bean tissues was evaluated.The highest L-DOPA content was detected in fresh leaves(22.4 mg g^(-1))followed by flowers,young pods,mature seeds,and roots.Regardless of processing method,L-DOPA concentration in faba bean tissues was significantly reduced when tissues were boiled or dried.Among various methods of processing,freezing had the lowest detrimental effect,reducing L-DOPA concentrations by 24.1%and 21.1%in leaves and seeds,respectively.Drought stress elevated L-DOPA concentration,and maximum L-DOPA(23.3 mg g^(-1)of biomass)was extracted from plants grown under severe drought stress.However,L-DOPA yield(L-DOPA concentration×biomass)was compromised,owing to the adverse influence of drought stress on dry matter production.No significant difference in L-DOPA concentration was detected among various N application rates.
基金the Key Technologies R&D Program of Hubei Province(No.2005AA301B14)
文摘Fe3O4 magnetic nanoparticles were prepared by co-precipitation of Fe^2＋ and Fe^3＋ in an ammonia solution, and its size was about 36 nm measured by an atomic force microscope. Fe3O4 magnetic nanoparticles were modified by L-dopa or dopamine using sonication method. The analysis of FTIR clearly indicated the formation of Fe-O-C bond. Direct immobilization of trypsin （EC： 3.4.21.4） on Fe3O4 magnetic nanoparticles with L-dopa and dopamine spacer was investigated using glutaraldehyde as a coupling agent. No significant changes in the size and magnetic property of the three kinds of magnetic nanoparticles linked with or without trypsin were observed. The existence of the spacer molecule on magnetic nanoparticles could greatly improve the activity and the storage stability of bound trypsin through increasing the flexibility of enzyme and changing the microenvironment on nanoparticles surface compared to the naked magnetic nanoparticles.
文摘Four new chiral 1,2,3,4-tetrahydroisoquinoline-derived β-amino alcohols were synthesized from L-DOPA in good yields. The structures of the target compounds were confirmed by ^1H NMR, ^13C NMR and MS.
文摘Parkinson’s disease was first formally identified by British physician James Parkinson in 1817 as “The Shaking Palsy”. L-DOPA (3,4-dihydroxy-phenyl-L-alanine) has been considered as a gold-standard treatment for Parkinson’s disease. The world market for L-DOPA is about 250 t/year and the total market volume is about $101 billion per year. The present review summarizes the different biological sources for the production of L-DOPA. The process for L-DOPA production from different biological sources has advantages over the chemical methods such as, enantiometrically pure L-DOPA, less incubation time and cost effective method. L-DOPA is found naturally in certain plant foods, particularly broad beans which found to replenish brain levels of L-DOPA even more quickly, and for longer periods, than conventional medication.
基金supported by Department of Biotechnology,Government of India for Interdisciplinary Programme of Life Sciences for the Advanced Research and Education(IPLS–Reference No:BT/PR4572/INF/22/147/2012)
文摘Objective: To investigate L-3, 4-dihydroxyphenylalanine(L-dopa, anti-Parkinson drug),anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa(M. macrocarpa) beans.Methods: L-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography(RPHPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential(2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid.Results: RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of L-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong antiinflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid(43.795 mg/g), gallic acid(0.864 mg/g), p-coumaric acid(0.364 mg/g) and p-hydroxybenzoic acid(0.036 mg/g) quantified successfully from M. macrocarpa beans respectively.Conclusions: This study suggests that M. macrocarpa is a potential source of L-dopa with promising anti-inflammatory, antioxidant and nutritional benefits.
