Presented in this paper are the newly obtained grain zircon U-Pb ages of volcanic rocks of the Luliang Group and associated Kuanping granitic migmatitic gneiss in Shanxi Province. The zircon U-Ph ages of bimodal volca...Presented in this paper are the newly obtained grain zircon U-Pb ages of volcanic rocks of the Luliang Group and associated Kuanping granitic migmatitic gneiss in Shanxi Province. The zircon U-Ph ages of bimodal volcanic rocks (basalt and rhyolite) of the Upper Luliang Group indicate that the rocks erupted at about 2100 Ma. So the Luliang Group was formed during the Early Proterozoic. In the area studied the second-stage metamorphism experienced by the Luliang Group is the dominant one which took place at about 1806 Ma, i. e., during the late Early Proterozoic.展开更多
Genus Grateloupia is one of the most speciose genera in family Halymeniales. It is also one of the sources for natural materials, food and medicine. With different environments, Grateloupia change their morphological ...Genus Grateloupia is one of the most speciose genera in family Halymeniales. It is also one of the sources for natural materials, food and medicine. With different environments, Grateloupia change their morphological characteristics, making their morphological identification very difficult. In addition, few of the species diversity in this genus has been described before. In this study, phylogenetic analysis based on rbc L gene sequence was employed to group Grateloupia collected from three locations along Chinese coast. The microsatellites were also used to evaluate their genetic diversity. In total, the tissue parts of 6 putative species were collected from G. asiatica, G. livida, G. lanceolate, G. catenata, G. turuturu and G. filicina. In order to evaluate their genetic diversity and then conserve them better, 40 microsatellites available for Grateloupia were used to evaluate their genetic diversity, and 11 microsatellites were found to be applicable to determine the genetic diversity of G. asiatica. It was found that the genetic diversity of G. asiatica around Qingdao was very rich. We suggested that the species of genus Grateloupia should be identified based on rbc L phylogenetic analysis before the diversity evaluation with microsatellites. The microsatellites should be developed for each species of Grateloupia so that their genetic diversity can be evaluated appropriately.展开更多
AIM： To investigate the effect of high mobility group protein box-1 （HMGB1） siRNA on proliferation and apoptosis of retinoblastoma （Rb） cells.METHODS： The expression of HMGB1 in Rb cells were detected by real-ti...AIM： To investigate the effect of high mobility group protein box-1 （HMGB1） siRNA on proliferation and apoptosis of retinoblastoma （Rb） cells.METHODS： The expression of HMGB1 in Rb cells were detected by real-time polymerase chain reaction （RT-PCR） and Western blot. Chemically synthesized HMGB1 siRNA was transfected into Y79 cells. The inhibitory rate was also examined by RT-PCR and Western blot. After HMGB1 siRNA transfection, the cell proliferation was analyzed by MTT, and cell apoptosis was detected by Caspase-3 active detection kit. Cell cycle distribution and apoptosis were detected by flow cytometry. RESULTS： The expression of HMGB1 significantly elevated in Rb cells （P〈0.01）. After transfected by siRNA, the HMGB1 protein level of Y79 cells was significantly reduced （P〈0.01）. After siRNA interference HMGB1, the proportion of proliferating cells reduced, and the proportion of quiescent cells increased （P〈0.05）. In addition, apoptosis rate of Y79 cells increased from 2.03% to 9.10% after interfering with HMGB1 siRNA （P〈0.05）.CONCLUSION： Specific HMGB1 siRNA can inhibit the expression of HMGB1. The effect may be attributed to inhibit the proliferation and promote cell apoptosis.展开更多
Liang Qichao 's analysis of "freedom "in On Freedom is mainly focused on revealing the evolution and variation of the term "freedom "in modern Chinese society. In his view,freedom is not only a...Liang Qichao 's analysis of "freedom "in On Freedom is mainly focused on revealing the evolution and variation of the term "freedom "in modern Chinese society. In his view,freedom is not only an indispensable element of an individual's life,but also fully applicable to the course of social and historical developm ent. Liang Qichao 's liberalism originates directly from the West,but is deeply influenced by the Chinese traditional discourse system and the overwhelming reality context of saving the nation in modern tim es.What is different from Western liberalism is that the dim ensions of Liang Qichao 's freedom are developed at two levels,one is the individual level,the other is the national level. The form er has the significance of enlightenm ent,while the latter has the significance of saving lives. Enlightenm ent and salvation are not only the"double variations"of modern C hina,but also the dual responsibility of modern Chinese enlightenm ent thinkers,which is dem-onstrated in Liang Qichao 's thought of freedom.展开更多
Let G be an l subgroup of a complete lattice ordered group H . For Conrad and McAlisters open question: to characterise those l subgroups of a complete l group H that have a unique completion...Let G be an l subgroup of a complete lattice ordered group H . For Conrad and McAlisters open question: to characterise those l subgroups of a complete l group H that have a unique completion in H , a necessary and sufficient condition on G is given so that G has a unique completion in H .展开更多
To unravel the geochemical heterogeneity and its origin in different terranes of North China,we conducted geochronological and geochemical analyses of the meta-mafic rocks from the Lüliang–Zhongtiao rift zone(Sh...To unravel the geochemical heterogeneity and its origin in different terranes of North China,we conducted geochronological and geochemical analyses of the meta-mafic rocks from the Lüliang–Zhongtiao rift zone(Shanxi Province).LA-ICP-MS zircon U–Pb dating yielded mostly End-Neoarchean to Proterozoic ages for the basement rocks(Sushui Complex:2516±26 Ma;Metamafic rocks:2494±31 Ma),Jiangxian Group(~2213 Ma),Zhongtiao Group(2077±29 Ma),Jiehekou Group(1998±23 Ma),and Lüliang Group(2152±52 Ma).Petrographic characteristics show that the meta-mafic rocks from the Neoarchean–Paleoproterozoic Zhongtiaoshan(Sushui Complex)have similar geochemical characteristics to the overlying Jiangxian and Zhongtiao Groups.The Paleoproterozoic Lüliang andYejishan Group meta-mafic rocks from Lüliangshan also have similar geochemical characteristics but are geochemically different from similar-age rocks from Zhongtiaoshan.This shows that the late-stage rocks have a geochemical inheritance from the early-stage rocks in the same region and that the geochemical heterogeneity of rocks from different areas was originated from the inherited heterogeneity of the magma source.展开更多
[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone ...[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.展开更多
文摘Presented in this paper are the newly obtained grain zircon U-Pb ages of volcanic rocks of the Luliang Group and associated Kuanping granitic migmatitic gneiss in Shanxi Province. The zircon U-Ph ages of bimodal volcanic rocks (basalt and rhyolite) of the Upper Luliang Group indicate that the rocks erupted at about 2100 Ma. So the Luliang Group was formed during the Early Proterozoic. In the area studied the second-stage metamorphism experienced by the Luliang Group is the dominant one which took place at about 1806 Ma, i. e., during the late Early Proterozoic.
基金supported by the Marine Industry Research Special Funds for Public Welfare Projects(No.201205024)
文摘Genus Grateloupia is one of the most speciose genera in family Halymeniales. It is also one of the sources for natural materials, food and medicine. With different environments, Grateloupia change their morphological characteristics, making their morphological identification very difficult. In addition, few of the species diversity in this genus has been described before. In this study, phylogenetic analysis based on rbc L gene sequence was employed to group Grateloupia collected from three locations along Chinese coast. The microsatellites were also used to evaluate their genetic diversity. In total, the tissue parts of 6 putative species were collected from G. asiatica, G. livida, G. lanceolate, G. catenata, G. turuturu and G. filicina. In order to evaluate their genetic diversity and then conserve them better, 40 microsatellites available for Grateloupia were used to evaluate their genetic diversity, and 11 microsatellites were found to be applicable to determine the genetic diversity of G. asiatica. It was found that the genetic diversity of G. asiatica around Qingdao was very rich. We suggested that the species of genus Grateloupia should be identified based on rbc L phylogenetic analysis before the diversity evaluation with microsatellites. The microsatellites should be developed for each species of Grateloupia so that their genetic diversity can be evaluated appropriately.
文摘AIM： To investigate the effect of high mobility group protein box-1 （HMGB1） siRNA on proliferation and apoptosis of retinoblastoma （Rb） cells.METHODS： The expression of HMGB1 in Rb cells were detected by real-time polymerase chain reaction （RT-PCR） and Western blot. Chemically synthesized HMGB1 siRNA was transfected into Y79 cells. The inhibitory rate was also examined by RT-PCR and Western blot. After HMGB1 siRNA transfection, the cell proliferation was analyzed by MTT, and cell apoptosis was detected by Caspase-3 active detection kit. Cell cycle distribution and apoptosis were detected by flow cytometry. RESULTS： The expression of HMGB1 significantly elevated in Rb cells （P〈0.01）. After transfected by siRNA, the HMGB1 protein level of Y79 cells was significantly reduced （P〈0.01）. After siRNA interference HMGB1, the proportion of proliferating cells reduced, and the proportion of quiescent cells increased （P〈0.05）. In addition, apoptosis rate of Y79 cells increased from 2.03% to 9.10% after interfering with HMGB1 siRNA （P〈0.05）.CONCLUSION： Specific HMGB1 siRNA can inhibit the expression of HMGB1. The effect may be attributed to inhibit the proliferation and promote cell apoptosis.
文摘Liang Qichao 's analysis of "freedom "in On Freedom is mainly focused on revealing the evolution and variation of the term "freedom "in modern Chinese society. In his view,freedom is not only an indispensable element of an individual's life,but also fully applicable to the course of social and historical developm ent. Liang Qichao 's liberalism originates directly from the West,but is deeply influenced by the Chinese traditional discourse system and the overwhelming reality context of saving the nation in modern tim es.What is different from Western liberalism is that the dim ensions of Liang Qichao 's freedom are developed at two levels,one is the individual level,the other is the national level. The form er has the significance of enlightenm ent,while the latter has the significance of saving lives. Enlightenm ent and salvation are not only the"double variations"of modern C hina,but also the dual responsibility of modern Chinese enlightenm ent thinkers,which is dem-onstrated in Liang Qichao 's thought of freedom.
文摘Let G be an l subgroup of a complete lattice ordered group H . For Conrad and McAlisters open question: to characterise those l subgroups of a complete l group H that have a unique completion in H , a necessary and sufficient condition on G is given so that G has a unique completion in H .
基金supported by the Strategic Priority Research Program (B) of Chinese Academy of Sciences (Grant No.XDB18010105)the Talent start-up fund of Guiyang University(2019039510821)。
文摘To unravel the geochemical heterogeneity and its origin in different terranes of North China,we conducted geochronological and geochemical analyses of the meta-mafic rocks from the Lüliang–Zhongtiao rift zone(Shanxi Province).LA-ICP-MS zircon U–Pb dating yielded mostly End-Neoarchean to Proterozoic ages for the basement rocks(Sushui Complex:2516±26 Ma;Metamafic rocks:2494±31 Ma),Jiangxian Group(~2213 Ma),Zhongtiao Group(2077±29 Ma),Jiehekou Group(1998±23 Ma),and Lüliang Group(2152±52 Ma).Petrographic characteristics show that the meta-mafic rocks from the Neoarchean–Paleoproterozoic Zhongtiaoshan(Sushui Complex)have similar geochemical characteristics to the overlying Jiangxian and Zhongtiao Groups.The Paleoproterozoic Lüliang andYejishan Group meta-mafic rocks from Lüliangshan also have similar geochemical characteristics but are geochemically different from similar-age rocks from Zhongtiaoshan.This shows that the late-stage rocks have a geochemical inheritance from the early-stage rocks in the same region and that the geochemical heterogeneity of rocks from different areas was originated from the inherited heterogeneity of the magma source.
文摘[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.
