Tree-ring width chronologies of Larix chinensis were developed from the northern and southern slopes of the Qinling Mountains in Shaanxi Province,and climatic factors affecting the tree-ring widths of L.chinensis were...Tree-ring width chronologies of Larix chinensis were developed from the northern and southern slopes of the Qinling Mountains in Shaanxi Province,and climatic factors affecting the tree-ring widths of L.chinensis were examined.Correlation analysis showed that similar correlations between tree-ring width chronologies and climatic factors demonstrated that radial growth responded to climate change on both slopes.The radial growth of L.chinensis was mainly limited by temperature,especially the growing season.In contrast,both chronologies were negatively correlated with precipitation in May of the previous year and April of the current year.Spatial climate-correlation analyses with gridded land-surface climate data revealed that our tree-ring width chronologies contained a strong regional temperature signal over much of northcentral and eastern China.Spatial correlation with seasurface temperature fields highlights the influence of the Pacific Ocean,Indian Ocean,and North Atlantic Ocean.Wavelet coherence analysis indicated the existence of some decadal and interannual cycles in the two tree-ring width chronologies.This may suggest the influences of El Nin˜o-Southern Oscillation and solar activity on tree growth in the Qinling Mountains.These findings will help us understand the growth response of L.chinensis to climate change in the Qinling region,and they provide critical information for future climate reconstructions based on this species in semi-humid regions.展开更多
Grassland is a major carbon sink in the terrestrial ecosystem. The dynamics of grassland carbon stock profoundly influence the global carbon cycle. In the published literatures so far, however, there are limited studi...Grassland is a major carbon sink in the terrestrial ecosystem. The dynamics of grassland carbon stock profoundly influence the global carbon cycle. In the published literatures so far, however, there are limited studies on the long-term dynamics and influential factors of grassland carbon stock, including soil organic carbon. In this study, spatial-temporal substitution method was applied to explore the characteristics of Medicago sativa L. (alfalfa) grassland biomass carbon and soil organic carbon density (SOCD) in a loess hilly region with different growing years and management patterns. The results demonstrated that alfalfa was the mono-dominant community during the cutting period (viz. 0-10 year). Community succession began after the abandonment of alfalfa grassland and then the important value of alfalfa in the community declined. The artificial alfalfa community abandoned for 30 years was replaced by the S. bungeana community. Accordingly, the biomass carbon density of the clipped alfalfa showed a significant increase over the time during 0-10 year. During 0-30 year, the SOCD from 0-100 cm of the soil layer of all 5 management patterns increased over time with a range between 5.300 ± 0.981 kg/m2 and 12.578 ± 0.863 kg/m2. The sloping croplands had the lowest SOCD at 5.300 ± 0.981 kg/m2 which was quite different from the abandoned grasslands growing for 30 years which exhibited the highest SOCD with 12.578 ± 0.863 kg/m2. The ecosystem carbon density of the grassland clipped for 2 years increased 0.1 kg/m2 compared with the sloping cropland, while that of the grassland clipped for 10 years substantially increased to 10.30 ± 1.26 kg/m2. Moreover, the ecosystem carbon density for abandoned grassland became 12.62± 0.50 kg/m2 at 30 years. The carbon density of the grassland undisturbed for l0 years was similar to that of the sloping cropland and the grassland clipped for 2 years. Different management patterns imposed great different effects on the accumulation of biomass carbon on artificial grasslands, whereas the ecosystem carbon density of the grassland showed a slight increase from the clipping to abandonment of grassland in general.展开更多
To unravel the geochemical heterogeneity and its origin in different terranes of North China,we conducted geochronological and geochemical analyses of the meta-mafic rocks from the Lüliang–Zhongtiao rift zone(Sh...To unravel the geochemical heterogeneity and its origin in different terranes of North China,we conducted geochronological and geochemical analyses of the meta-mafic rocks from the Lüliang–Zhongtiao rift zone(Shanxi Province).LA-ICP-MS zircon U–Pb dating yielded mostly End-Neoarchean to Proterozoic ages for the basement rocks(Sushui Complex:2516±26 Ma;Metamafic rocks:2494±31 Ma),Jiangxian Group(~2213 Ma),Zhongtiao Group(2077±29 Ma),Jiehekou Group(1998±23 Ma),and Lüliang Group(2152±52 Ma).Petrographic characteristics show that the meta-mafic rocks from the Neoarchean–Paleoproterozoic Zhongtiaoshan(Sushui Complex)have similar geochemical characteristics to the overlying Jiangxian and Zhongtiao Groups.The Paleoproterozoic Lüliang andYejishan Group meta-mafic rocks from Lüliangshan also have similar geochemical characteristics but are geochemically different from similar-age rocks from Zhongtiaoshan.This shows that the late-stage rocks have a geochemical inheritance from the early-stage rocks in the same region and that the geochemical heterogeneity of rocks from different areas was originated from the inherited heterogeneity of the magma source.展开更多
Populations of the European brown bear (Ursus arctos L.) differ substantially in size, degree of geographic isolation and level of genetic diversity. Present patterns result from phylogeographic processes and profound...Populations of the European brown bear (Ursus arctos L.) differ substantially in size, degree of geographic isolation and level of genetic diversity. Present patterns result from phylogeographic processes and profound human intervention. We assessed the genetic variability of a subpopulation of brown bears near the periphery of their range in the Western Carpathian Mountains and compared their genetic properties with those of bears in the core of the same population and elsewhere. Samples were collected non-invasively in 2007-2008 and2010 in Strázovské Vrchy Protected Landscape Area (PLA) in Slovakia (included in the NATURA 2000 networking programme). Seven polymorphic microsatellite loci (UaMU26, UaMU64, G10B, G1D, G10L, UaMU50 and UaMU51) were amplified using a nested PCR in order to assess the following parameters: variability, allelic combinations, heterozygosity, number of alleles and inbreeding coefficient. Sufficient brown bear DNA for analysis was obtained from 57 out of 140 samples (41%), among which 45 different genotypes were identified. Loci had a mean of 2.71 ± 0.76 alleles. Average observed heterozygosity was 0.59. The inbreeding coefficient was negative for all but one of the analysed loci (2007-2008). In the year 2010 was negative three of seven loci. These results imply that gene flow with other parts of the population has been maintained in the reduced level and the isolation level of bears in the study area was not so low. Nevertheless, the genetic variability of bears in Strázovské Vrchy PLA was lower than that reported from other localities in the Carpathian Mountains. The results are discussed in the context of behavioural ecology and conservation genetics.展开更多
The Hengduan Mountains region of south-west China is a noted biodiversity,hotspot, but the geographic origins and historical assembly of its rich endemic flora, including the sky-island species ofSolms-laubachia Musch...The Hengduan Mountains region of south-west China is a noted biodiversity,hotspot, but the geographic origins and historical assembly of its rich endemic flora, including the sky-island species ofSolms-laubachia Muschl. (Brassicaceae), have been little studied. Previous molecular studies on the phylogeny of Solms-laubachia showed it to be paraphyletic, leading to considerable expansion not only of its taxonomic limits, but also its geographic range, with the inclusion of taxa from outside the Hengduan region. However, these studies provided little resolution of interspecific relationships, preventing inferences about historical biogeography within the clade. In the present study, new sequence data from two nuclear genes (LEAFY and G3pdh) and two chloroplast intergenic spacers (petN-psbM and psbM-trnD) were combined with existing markers to increase phylogenetic signals. Phaeonychium villosum (Maxim.) Al-Shehbaz was found to be nested within Solms-laubachia s.l. In general, phylogenetic relationships appear to be a good predictor of geography, with the Hengduan Mountain endemics embedded in a paraphyletic grade of species from the western Himalayas and central Asia, but they also imply morphological homoplasy, lncongruence was detected between the nuclear and chloroplast gene trees, perhaps resulting from incomplete lineage sorting of ancestral polymorphisms. The crown age ofSolms-laubachia s.l. was estimated to be approximately 1.42-3.68 mya, using Bayesian relaxed molecular clock analysis. Historical biogeographic analysis using a parametric dispersalextinction-cladogenesis model inferred central Asia and the western Himalayas as most probable ancestral range of Solms-laubachia s.l., and estimated higher rates of eastward expansion than westward during the diversification of descendant lineages. In summary, our results suggest that Solms-laubachia s.l. originated during the Pliocene in central Asia, and subsequently migrated eastward into the Hengduan Mountains, colonizing sky-island, alpine scree-slope habitats that may have provided novel ecological opportunity and accelerated speciation, ultimately establishing this region as the present center of diversity of the genus.展开更多
Ruta genus is a member of the family Rutaceae that has been cultivated widely in many regions of the world because of its medicinal properties. In Tessala Mountain (Sidi Bel Abbes Country, North-Western of Algeria),...Ruta genus is a member of the family Rutaceae that has been cultivated widely in many regions of the world because of its medicinal properties. In Tessala Mountain (Sidi Bel Abbes Country, North-Western of Algeria), the Ruta genus commonly known by "fidjel" is represented by two species: Ruta Chalepensis L. and Ruta Montana (L.) L. frequently used by local population in a traditional treatment. The water-distilled essential oils from this species yielded 7.23% and 6.104% for Ruta chalepensis L. and Ruta montana (L.) L. respectively. The essential oil of Ruta montana (L.) L. showed a strong antibacterial activity against all bacterial strains tested (Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Mycobacterium kansasii ATCC ! 2478 and Mycobacterium vaccae ATCC 1548314) compared to Ruta chalepensis L. oils which have a moderate effect only on Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Therefor, the antibacterial properties of the essential oils of Ruta Chalepensis L. and Ruta Montana (L.) L. are now well established through this study and therefore could justify their future uses in the treatment of nosocomial infections.展开更多
目的基于网络药理学和分子对接方法探究红景天防治急性高原病的作用机制。方法通过TCMID和ETCM数据库检索红景天的化学成分,再经过SwissTarget和STITCH数据库筛选红景天成分作用靶点;通过DisGeNET、Genecards及GEO数据库筛选急性高原病...目的基于网络药理学和分子对接方法探究红景天防治急性高原病的作用机制。方法通过TCMID和ETCM数据库检索红景天的化学成分,再经过SwissTarget和STITCH数据库筛选红景天成分作用靶点;通过DisGeNET、Genecards及GEO数据库筛选急性高原病相关靶点;利用STRING数据库获取蛋白质相互作用(Protein-protein interaction,PPI)网络;利用DAVID数据库进行基因本体(Gene ontology,GO)功能富集分析及京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析;采用Cytoscape软件构建“药物-成分-靶点-疾病”网络及“成分-靶点-通路”网络;利用Autodock软件进行分子对接研究;采用2018年国际路易斯湖评分系统对患者症状进行评分;使用ELISA试剂盒对患者血清IL6、IL10及HIF1A水平进行测定。结果从红景天中筛选得到31个化学成分和2295个作用靶点,与1083个急性高原病相关靶点取交集,得到125个共同靶点,PPI网络中心主要涉及IL6、VEGFA、CAT、IL10、IL2、HIF1A等关键靶蛋白。KEGG富集分析主要涉及HIF-1信号通路、PI3K-AKT信号通路、VEGF信号通路等相关通路。分子对接验证显示红景天成分槲皮素、山奈酚、没食子酸、咖啡酸、红景天苷与关键靶点IL6、VEGFA、CAT、IL2、HIF1A的结合活性较好。此外,急性高原病患者在服用红景天胶囊后头痛、胃肠道症状、疲乏以及头晕情况明显缓解,症状总评分显著降低;血清中IL6和HIF1A水平显著降低,IL10水平显著升高。结论红景天能够通过多成分、多靶点、多通路的方式,发挥防治急性高原病的作用。展开更多
基金funded by National Natural Science Foundation of China(No.31370587)
文摘Tree-ring width chronologies of Larix chinensis were developed from the northern and southern slopes of the Qinling Mountains in Shaanxi Province,and climatic factors affecting the tree-ring widths of L.chinensis were examined.Correlation analysis showed that similar correlations between tree-ring width chronologies and climatic factors demonstrated that radial growth responded to climate change on both slopes.The radial growth of L.chinensis was mainly limited by temperature,especially the growing season.In contrast,both chronologies were negatively correlated with precipitation in May of the previous year and April of the current year.Spatial climate-correlation analyses with gridded land-surface climate data revealed that our tree-ring width chronologies contained a strong regional temperature signal over much of northcentral and eastern China.Spatial correlation with seasurface temperature fields highlights the influence of the Pacific Ocean,Indian Ocean,and North Atlantic Ocean.Wavelet coherence analysis indicated the existence of some decadal and interannual cycles in the two tree-ring width chronologies.This may suggest the influences of El Nin˜o-Southern Oscillation and solar activity on tree growth in the Qinling Mountains.These findings will help us understand the growth response of L.chinensis to climate change in the Qinling region,and they provide critical information for future climate reconstructions based on this species in semi-humid regions.
基金Under the auspices of Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA05000000)National Natural Science Foundation of China(No.41271518)Sci-technology Project of Shaanxi Province(No.2013kw19-01)
文摘Grassland is a major carbon sink in the terrestrial ecosystem. The dynamics of grassland carbon stock profoundly influence the global carbon cycle. In the published literatures so far, however, there are limited studies on the long-term dynamics and influential factors of grassland carbon stock, including soil organic carbon. In this study, spatial-temporal substitution method was applied to explore the characteristics of Medicago sativa L. (alfalfa) grassland biomass carbon and soil organic carbon density (SOCD) in a loess hilly region with different growing years and management patterns. The results demonstrated that alfalfa was the mono-dominant community during the cutting period (viz. 0-10 year). Community succession began after the abandonment of alfalfa grassland and then the important value of alfalfa in the community declined. The artificial alfalfa community abandoned for 30 years was replaced by the S. bungeana community. Accordingly, the biomass carbon density of the clipped alfalfa showed a significant increase over the time during 0-10 year. During 0-30 year, the SOCD from 0-100 cm of the soil layer of all 5 management patterns increased over time with a range between 5.300 ± 0.981 kg/m2 and 12.578 ± 0.863 kg/m2. The sloping croplands had the lowest SOCD at 5.300 ± 0.981 kg/m2 which was quite different from the abandoned grasslands growing for 30 years which exhibited the highest SOCD with 12.578 ± 0.863 kg/m2. The ecosystem carbon density of the grassland clipped for 2 years increased 0.1 kg/m2 compared with the sloping cropland, while that of the grassland clipped for 10 years substantially increased to 10.30 ± 1.26 kg/m2. Moreover, the ecosystem carbon density for abandoned grassland became 12.62± 0.50 kg/m2 at 30 years. The carbon density of the grassland undisturbed for l0 years was similar to that of the sloping cropland and the grassland clipped for 2 years. Different management patterns imposed great different effects on the accumulation of biomass carbon on artificial grasslands, whereas the ecosystem carbon density of the grassland showed a slight increase from the clipping to abandonment of grassland in general.
基金supported by the Strategic Priority Research Program (B) of Chinese Academy of Sciences (Grant No.XDB18010105)the Talent start-up fund of Guiyang University(2019039510821)。
文摘To unravel the geochemical heterogeneity and its origin in different terranes of North China,we conducted geochronological and geochemical analyses of the meta-mafic rocks from the Lüliang–Zhongtiao rift zone(Shanxi Province).LA-ICP-MS zircon U–Pb dating yielded mostly End-Neoarchean to Proterozoic ages for the basement rocks(Sushui Complex:2516±26 Ma;Metamafic rocks:2494±31 Ma),Jiangxian Group(~2213 Ma),Zhongtiao Group(2077±29 Ma),Jiehekou Group(1998±23 Ma),and Lüliang Group(2152±52 Ma).Petrographic characteristics show that the meta-mafic rocks from the Neoarchean–Paleoproterozoic Zhongtiaoshan(Sushui Complex)have similar geochemical characteristics to the overlying Jiangxian and Zhongtiao Groups.The Paleoproterozoic Lüliang andYejishan Group meta-mafic rocks from Lüliangshan also have similar geochemical characteristics but are geochemically different from similar-age rocks from Zhongtiaoshan.This shows that the late-stage rocks have a geochemical inheritance from the early-stage rocks in the same region and that the geochemical heterogeneity of rocks from different areas was originated from the inherited heterogeneity of the magma source.
基金supported by Structural Funds of EU Project of the Agency of the Ministry of Education,Science,Research and Sport of the Slovak Republic,Bratislava,ITMS No.26110230078
文摘Populations of the European brown bear (Ursus arctos L.) differ substantially in size, degree of geographic isolation and level of genetic diversity. Present patterns result from phylogeographic processes and profound human intervention. We assessed the genetic variability of a subpopulation of brown bears near the periphery of their range in the Western Carpathian Mountains and compared their genetic properties with those of bears in the core of the same population and elsewhere. Samples were collected non-invasively in 2007-2008 and2010 in Strázovské Vrchy Protected Landscape Area (PLA) in Slovakia (included in the NATURA 2000 networking programme). Seven polymorphic microsatellite loci (UaMU26, UaMU64, G10B, G1D, G10L, UaMU50 and UaMU51) were amplified using a nested PCR in order to assess the following parameters: variability, allelic combinations, heterozygosity, number of alleles and inbreeding coefficient. Sufficient brown bear DNA for analysis was obtained from 57 out of 140 samples (41%), among which 45 different genotypes were identified. Loci had a mean of 2.71 ± 0.76 alleles. Average observed heterozygosity was 0.59. The inbreeding coefficient was negative for all but one of the analysed loci (2007-2008). In the year 2010 was negative three of seven loci. These results imply that gene flow with other parts of the population has been maintained in the reduced level and the isolation level of bears in the study area was not so low. Nevertheless, the genetic variability of bears in Strázovské Vrchy PLA was lower than that reported from other localities in the Carpathian Mountains. The results are discussed in the context of behavioural ecology and conservation genetics.
基金supported by the Chinese National Natural Science Foundation (grants no.30625004,40771073 to HS)the Yunnan Natural Science Foundation (2008CC013 to HS)+4 种基金the US National Science Foundation (grant no. DEB-0321846,to DEB)the John D. and Catherine T. MacArthur Foundation (grant to JW,RR,and GM)the Innovation Project of the Chinese Academy of Sciences (KSCX2-YW-Z-030)a Mercer Fellowship from the Arnold Arboretum (to JPY)supported by the National Geographic Society (grant no.7405-03)
文摘The Hengduan Mountains region of south-west China is a noted biodiversity,hotspot, but the geographic origins and historical assembly of its rich endemic flora, including the sky-island species ofSolms-laubachia Muschl. (Brassicaceae), have been little studied. Previous molecular studies on the phylogeny of Solms-laubachia showed it to be paraphyletic, leading to considerable expansion not only of its taxonomic limits, but also its geographic range, with the inclusion of taxa from outside the Hengduan region. However, these studies provided little resolution of interspecific relationships, preventing inferences about historical biogeography within the clade. In the present study, new sequence data from two nuclear genes (LEAFY and G3pdh) and two chloroplast intergenic spacers (petN-psbM and psbM-trnD) were combined with existing markers to increase phylogenetic signals. Phaeonychium villosum (Maxim.) Al-Shehbaz was found to be nested within Solms-laubachia s.l. In general, phylogenetic relationships appear to be a good predictor of geography, with the Hengduan Mountain endemics embedded in a paraphyletic grade of species from the western Himalayas and central Asia, but they also imply morphological homoplasy, lncongruence was detected between the nuclear and chloroplast gene trees, perhaps resulting from incomplete lineage sorting of ancestral polymorphisms. The crown age ofSolms-laubachia s.l. was estimated to be approximately 1.42-3.68 mya, using Bayesian relaxed molecular clock analysis. Historical biogeographic analysis using a parametric dispersalextinction-cladogenesis model inferred central Asia and the western Himalayas as most probable ancestral range of Solms-laubachia s.l., and estimated higher rates of eastward expansion than westward during the diversification of descendant lineages. In summary, our results suggest that Solms-laubachia s.l. originated during the Pliocene in central Asia, and subsequently migrated eastward into the Hengduan Mountains, colonizing sky-island, alpine scree-slope habitats that may have provided novel ecological opportunity and accelerated speciation, ultimately establishing this region as the present center of diversity of the genus.
文摘Ruta genus is a member of the family Rutaceae that has been cultivated widely in many regions of the world because of its medicinal properties. In Tessala Mountain (Sidi Bel Abbes Country, North-Western of Algeria), the Ruta genus commonly known by "fidjel" is represented by two species: Ruta Chalepensis L. and Ruta Montana (L.) L. frequently used by local population in a traditional treatment. The water-distilled essential oils from this species yielded 7.23% and 6.104% for Ruta chalepensis L. and Ruta montana (L.) L. respectively. The essential oil of Ruta montana (L.) L. showed a strong antibacterial activity against all bacterial strains tested (Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Mycobacterium kansasii ATCC ! 2478 and Mycobacterium vaccae ATCC 1548314) compared to Ruta chalepensis L. oils which have a moderate effect only on Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Therefor, the antibacterial properties of the essential oils of Ruta Chalepensis L. and Ruta Montana (L.) L. are now well established through this study and therefore could justify their future uses in the treatment of nosocomial infections.
文摘目的基于网络药理学和分子对接方法探究红景天防治急性高原病的作用机制。方法通过TCMID和ETCM数据库检索红景天的化学成分,再经过SwissTarget和STITCH数据库筛选红景天成分作用靶点;通过DisGeNET、Genecards及GEO数据库筛选急性高原病相关靶点;利用STRING数据库获取蛋白质相互作用(Protein-protein interaction,PPI)网络;利用DAVID数据库进行基因本体(Gene ontology,GO)功能富集分析及京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析;采用Cytoscape软件构建“药物-成分-靶点-疾病”网络及“成分-靶点-通路”网络;利用Autodock软件进行分子对接研究;采用2018年国际路易斯湖评分系统对患者症状进行评分;使用ELISA试剂盒对患者血清IL6、IL10及HIF1A水平进行测定。结果从红景天中筛选得到31个化学成分和2295个作用靶点,与1083个急性高原病相关靶点取交集,得到125个共同靶点,PPI网络中心主要涉及IL6、VEGFA、CAT、IL10、IL2、HIF1A等关键靶蛋白。KEGG富集分析主要涉及HIF-1信号通路、PI3K-AKT信号通路、VEGF信号通路等相关通路。分子对接验证显示红景天成分槲皮素、山奈酚、没食子酸、咖啡酸、红景天苷与关键靶点IL6、VEGFA、CAT、IL2、HIF1A的结合活性较好。此外,急性高原病患者在服用红景天胶囊后头痛、胃肠道症状、疲乏以及头晕情况明显缓解,症状总评分显著降低;血清中IL6和HIF1A水平显著降低,IL10水平显著升高。结论红景天能够通过多成分、多靶点、多通路的方式,发挥防治急性高原病的作用。