Opiate dependence has become one of the most urgent problems of modernsociety. Opiate dependence involves physical and psychical dependence. Although many addicts can bedetoxified, the relapse ratio of 95% in 5 a demo...Opiate dependence has become one of the most urgent problems of modernsociety. Opiate dependence involves physical and psychical dependence. Although many addicts can bedetoxified, the relapse ratio of 95% in 5 a demonstrates that opiate psychical dependence is aproblem more troublesome. It has been reported that acute and chronic administration of L- NNA canmarkedly retard the development of tolerance to physical dependence on morphine, and suppress theabstinence syndromes precipitated by naloxone in opiate dependent rodents, and even reverse theexisting morphine tolerance. However, the effect of L-NNA on the positive reinforcement ofpsychically active substances and its possible mechanism have not yet been reported. In presentstudy, the effect of L- NNA en the psychical dependence induced by opiates was evaluated on thebasis of conditioned place preference.展开更多
AIML To investigate the effect and mechanism of action of the nitric oxide synthase （NOS） inhibitor NG-nitro-L-arginine methyl ester （L-NAME） on invasion and metastasis of human colorectal cancer cell line SL-174T...AIML To investigate the effect and mechanism of action of the nitric oxide synthase （NOS） inhibitor NG-nitro-L-arginine methyl ester （L-NAME） on invasion and metastasis of human colorectal cancer cell line SL-174T. METHODS： Human colorectal cancer cel4 line SL-174T was cultured and treated separately with four different dosages of L-NAME for 72 h, Nitric oxide （NO） production was measured with Griess reagent, The effect of L-NAME on invasion and migration of SL-174T cells were evaluated by using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane （Matrigel）, RT-PCR was performed to determine the mRNA levels of matrix metalloproteinase-2 （MMP-2） and tissue inhibitor metalloproteinase-2 （TIMP-2）,RESULTS： L-NAME could significantly inhibit NO production of SL-174T in a dose-dependent manner. After being treated for 72 h with 0.2, 0.4, 0.8, and 1.0 mmol/L L- NAME, respectively, the ability of the L-NAME treated SL- 174T cells to invade the reconstituted basement membrane decreased significantly （t = 8.056, P〈0.05; t= 14.467, P〈0.01; t= 27.785, P〈0.01; and t= 29.405, P〈0.01, respectively） and the inhibition rates were 10.29%, 19.62%, 34.08%, and 42.23%, respectively. Moreover, L-NAME could inhibit migration of SL-174T cells, and the inhibition rates were 20.76%, 24.95%, 39.43%, and 46. 85% for L-NAME at 0.2, 0.4, 0.8, and 1.0 mmol/L, respectively （t = 15.116, P〈0.01）. In addition, after treatment with L-NAME, expression of MMP-2 mRNA was significantly decreased （t = 71.238, P〈0.01） and that of TIMP-2 mRNA was markedly increased （t = -13.020, P〈0.01）. CONCLUSION： L-NAME exerts anti-invasive and anti- metastatic effects on SL-174T cell line via downregulating MNP-2 mRNA expression and upregulating TIMP-2 mRNA expression.展开更多
目的观察硫化氢（H2S）预处理对高血压大鼠主动脉功能的影响。方法健康雄性SD大鼠，随机分为4组（每组n=10）。①对照组，自由饮自来水；②硫氢化钠（NaHS）组，腹腔注射NaHS溶液（10μmol·kg^-·d^-1）；③L-硝基-精氨酸甲酯...目的观察硫化氢（H2S）预处理对高血压大鼠主动脉功能的影响。方法健康雄性SD大鼠，随机分为4组（每组n=10）。①对照组，自由饮自来水；②硫氢化钠（NaHS）组，腹腔注射NaHS溶液（10μmol·kg^-·d^-1）；③L-硝基-精氨酸甲酯（L-NAME）组，L-NAME溶于自来水自由饮水喂养；④NaHS＋L—NAME组，腹腔注射NaHS溶液（10μmol·kg^-1·d^-1），L—NAME溶于自来水自由喂养。每组处理时间为14天。PowerLab系统测定大鼠离体主动脉血管舒张功能；Western blot测定大鼠主动脉胱硫醚-γ-裂解酶（CSE）的表达；生化方法测定大鼠血清H2S浓度；苏木精-伊红染色、Elastic Van Gieson（EVG）染色观察大鼠主动脉管壁厚度、管壁结构、弹性纤维及胶原纤维的变化。结果与对照组比较，L-NAME组大鼠血管舒张功能明显减弱（P〈0．05），血清H2S浓度降低（P〈0．05），主动脉CSE的表达减少（P〈0．05）。NaHS预处理14天可以逆转L—NAME的作用，上调主动脉CSE的表达，增加血清中H2S的浓度，改善乙酰胆碱（ACh）引起的内皮依赖性血管舒张作用（P〈0．05）。但是各组大鼠主动脉管壁厚度、管壁结构、胶原纤维和弹性纤维等未见明显变化。结论外源性NaHS预处理可以上调大鼠主动脉中CSE的表达，增加血清H2S的浓度，提高主动脉的舒张功能。展开更多
基金ThisprojectwassupportedbyGrantofthePLALaboratoryforNewDrugEvaluation (No .96-0 2 )
文摘Opiate dependence has become one of the most urgent problems of modernsociety. Opiate dependence involves physical and psychical dependence. Although many addicts can bedetoxified, the relapse ratio of 95% in 5 a demonstrates that opiate psychical dependence is aproblem more troublesome. It has been reported that acute and chronic administration of L- NNA canmarkedly retard the development of tolerance to physical dependence on morphine, and suppress theabstinence syndromes precipitated by naloxone in opiate dependent rodents, and even reverse theexisting morphine tolerance. However, the effect of L-NNA on the positive reinforcement ofpsychically active substances and its possible mechanism have not yet been reported. In presentstudy, the effect of L- NNA en the psychical dependence induced by opiates was evaluated on thebasis of conditioned place preference.
文摘AIML To investigate the effect and mechanism of action of the nitric oxide synthase （NOS） inhibitor NG-nitro-L-arginine methyl ester （L-NAME） on invasion and metastasis of human colorectal cancer cell line SL-174T. METHODS： Human colorectal cancer cel4 line SL-174T was cultured and treated separately with four different dosages of L-NAME for 72 h, Nitric oxide （NO） production was measured with Griess reagent, The effect of L-NAME on invasion and migration of SL-174T cells were evaluated by using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane （Matrigel）, RT-PCR was performed to determine the mRNA levels of matrix metalloproteinase-2 （MMP-2） and tissue inhibitor metalloproteinase-2 （TIMP-2）,RESULTS： L-NAME could significantly inhibit NO production of SL-174T in a dose-dependent manner. After being treated for 72 h with 0.2, 0.4, 0.8, and 1.0 mmol/L L- NAME, respectively, the ability of the L-NAME treated SL- 174T cells to invade the reconstituted basement membrane decreased significantly （t = 8.056, P〈0.05; t= 14.467, P〈0.01; t= 27.785, P〈0.01; and t= 29.405, P〈0.01, respectively） and the inhibition rates were 10.29%, 19.62%, 34.08%, and 42.23%, respectively. Moreover, L-NAME could inhibit migration of SL-174T cells, and the inhibition rates were 20.76%, 24.95%, 39.43%, and 46. 85% for L-NAME at 0.2, 0.4, 0.8, and 1.0 mmol/L, respectively （t = 15.116, P〈0.01）. In addition, after treatment with L-NAME, expression of MMP-2 mRNA was significantly decreased （t = 71.238, P〈0.01） and that of TIMP-2 mRNA was markedly increased （t = -13.020, P〈0.01）. CONCLUSION： L-NAME exerts anti-invasive and anti- metastatic effects on SL-174T cell line via downregulating MNP-2 mRNA expression and upregulating TIMP-2 mRNA expression.
文摘目的观察硫化氢（H2S）预处理对高血压大鼠主动脉功能的影响。方法健康雄性SD大鼠，随机分为4组（每组n=10）。①对照组，自由饮自来水；②硫氢化钠（NaHS）组，腹腔注射NaHS溶液（10μmol·kg^-·d^-1）；③L-硝基-精氨酸甲酯（L-NAME）组，L-NAME溶于自来水自由饮水喂养；④NaHS＋L—NAME组，腹腔注射NaHS溶液（10μmol·kg^-1·d^-1），L—NAME溶于自来水自由喂养。每组处理时间为14天。PowerLab系统测定大鼠离体主动脉血管舒张功能；Western blot测定大鼠主动脉胱硫醚-γ-裂解酶（CSE）的表达；生化方法测定大鼠血清H2S浓度；苏木精-伊红染色、Elastic Van Gieson（EVG）染色观察大鼠主动脉管壁厚度、管壁结构、弹性纤维及胶原纤维的变化。结果与对照组比较，L-NAME组大鼠血管舒张功能明显减弱（P〈0．05），血清H2S浓度降低（P〈0．05），主动脉CSE的表达减少（P〈0．05）。NaHS预处理14天可以逆转L—NAME的作用，上调主动脉CSE的表达，增加血清中H2S的浓度，改善乙酰胆碱（ACh）引起的内皮依赖性血管舒张作用（P〈0．05）。但是各组大鼠主动脉管壁厚度、管壁结构、胶原纤维和弹性纤维等未见明显变化。结论外源性NaHS预处理可以上调大鼠主动脉中CSE的表达，增加血清H2S的浓度，提高主动脉的舒张功能。
