Improved synthesis and structure identification of L-histidine norcantharimide , a potent PP2A inhibitor was reported. Condensation between norcantharidin and L-histidine in 95% EtOH at reflux temperature affords L-hi...Improved synthesis and structure identification of L-histidine norcantharimide , a potent PP2A inhibitor was reported. Condensation between norcantharidin and L-histidine in 95% EtOH at reflux temperature affords L-histidine norcantharimide in 97.0f% yield which is much higher compared with literature, and more importantly, the configuration is retained. The chemical structure of the compound was re-elucidated through IR, FAB-MS, ^1H NMR, ^13C NMR and 2D NMR （^1H, ^13C-COSY and HMBC）, the fundamental physical data, including optical data being also firstly reported. Preliminary cytotoxicity evaluation showed that the target compound was probably more potent than norcantharidin against a panel of human cancer cell lines. Design and synthesis of amino acid （nor） cantharirnides would provide a convenient and rational structure modification of （nor） cantharidin and open new avenues to explore new promising candidates.展开更多
A novel nickel（Ⅱ） complex with L-histidine has been synthesized and solved by single-crystal X-ray diffraction analysis at physiological pH. The title complex （C7H16NiN4O6S, Mr = 343.01） crystallizes in monoclini...A novel nickel（Ⅱ） complex with L-histidine has been synthesized and solved by single-crystal X-ray diffraction analysis at physiological pH. The title complex （C7H16NiN4O6S, Mr = 343.01） crystallizes in monoclinic, space group P21 with a = 7.2194（7）, b = 7.5968（7）, c = 12.2797（11） A, β = 93.3110（10）°, V = 672.35（11） A^3, Z = 2, Dc= 1.694 g/cm^3, F（000） = 356, μ（MoKα） = 1.626 mm^-1, T = 293（2） K, the final R = 0.0184 and wR = 0.0426 for 2207 observed reflections with 1 〉 2σ（I）. The complex provides insights into a possible structural arrangement between nickel （Ⅱ） and L-histidine which may be physiologically important and abundantly present in biological systems.展开更多
The ternary complexes containing Cu(II),L-His and nucleotide (5'-GMP and 5'-IMP)were synthesized and characterized.IR and ~1H NMR spectra show that Cu(II)binds to carboxylate oxygen and imidazole nitrogen of L...The ternary complexes containing Cu(II),L-His and nucleotide (5'-GMP and 5'-IMP)were synthesized and characterized.IR and ~1H NMR spectra show that Cu(II)binds to carboxylate oxygen and imidazole nitrogen of L-His and purine N_7 of 5'-GMP and 5'-IMP.The interaction of Cu(II)with Po_3^(2-)of 5'-GMP is present,but that for 5'-IMP is not present.展开更多
Three novel L-histidine amide derivatives were synthesized and the corresponding chemical structures were characterized by means of melting point analysis, IR, MS, ^1H NMR as well as ^13C NMR. The coagulation activiti...Three novel L-histidine amide derivatives were synthesized and the corresponding chemical structures were characterized by means of melting point analysis, IR, MS, ^1H NMR as well as ^13C NMR. The coagulation activities of the compounds were evaluated by an MOE（molecular operating environment） docking technique and coagulation test. The results obtained from molecular docking show that the interactions between the compounds and thrombin exhibit procoagulant activity in combination with an improved combinatory effect. Moreover, the results of in vitro coagulation tests show that the L-histidine amide derivatives feature coagulant activities in common coagulation pathways. Compared with the blank control group, the optimal shortening rates of compounds 1-3 were 39.08%（0.5 mmol/L）, 22.94%（1.0 mmol/L） and 15.38%（0.0625 mmol/L）, respectively.展开更多
基金Science and Technology Program of Guangdong Province(Grant No.2006B35604002)Guangzhou City(Grant No.2007JI-C0261).
文摘Improved synthesis and structure identification of L-histidine norcantharimide , a potent PP2A inhibitor was reported. Condensation between norcantharidin and L-histidine in 95% EtOH at reflux temperature affords L-histidine norcantharimide in 97.0f% yield which is much higher compared with literature, and more importantly, the configuration is retained. The chemical structure of the compound was re-elucidated through IR, FAB-MS, ^1H NMR, ^13C NMR and 2D NMR （^1H, ^13C-COSY and HMBC）, the fundamental physical data, including optical data being also firstly reported. Preliminary cytotoxicity evaluation showed that the target compound was probably more potent than norcantharidin against a panel of human cancer cell lines. Design and synthesis of amino acid （nor） cantharirnides would provide a convenient and rational structure modification of （nor） cantharidin and open new avenues to explore new promising candidates.
基金This work was supported by the National Natural Science Foundation of China (No. 50572040)
文摘A novel nickel（Ⅱ） complex with L-histidine has been synthesized and solved by single-crystal X-ray diffraction analysis at physiological pH. The title complex （C7H16NiN4O6S, Mr = 343.01） crystallizes in monoclinic, space group P21 with a = 7.2194（7）, b = 7.5968（7）, c = 12.2797（11） A, β = 93.3110（10）°, V = 672.35（11） A^3, Z = 2, Dc= 1.694 g/cm^3, F（000） = 356, μ（MoKα） = 1.626 mm^-1, T = 293（2） K, the final R = 0.0184 and wR = 0.0426 for 2207 observed reflections with 1 〉 2σ（I）. The complex provides insights into a possible structural arrangement between nickel （Ⅱ） and L-histidine which may be physiologically important and abundantly present in biological systems.
文摘The ternary complexes containing Cu(II),L-His and nucleotide (5'-GMP and 5'-IMP)were synthesized and characterized.IR and ~1H NMR spectra show that Cu(II)binds to carboxylate oxygen and imidazole nitrogen of L-His and purine N_7 of 5'-GMP and 5'-IMP.The interaction of Cu(II)with Po_3^(2-)of 5'-GMP is present,but that for 5'-IMP is not present.
基金Supported by the National Natural Science Foundation of China（No.21362001）, the Project of the Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, China（No.guizhongzhongkai201104） and the High Level Innovation Team and Outstanding Scholar Project of Guangxi Institutions of Higher Education, China（No.guijiaoren[2014]49）.
文摘Three novel L-histidine amide derivatives were synthesized and the corresponding chemical structures were characterized by means of melting point analysis, IR, MS, ^1H NMR as well as ^13C NMR. The coagulation activities of the compounds were evaluated by an MOE（molecular operating environment） docking technique and coagulation test. The results obtained from molecular docking show that the interactions between the compounds and thrombin exhibit procoagulant activity in combination with an improved combinatory effect. Moreover, the results of in vitro coagulation tests show that the L-histidine amide derivatives feature coagulant activities in common coagulation pathways. Compared with the blank control group, the optimal shortening rates of compounds 1-3 were 39.08%（0.5 mmol/L）, 22.94%（1.0 mmol/L） and 15.38%（0.0625 mmol/L）, respectively.
