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石榴花中多酚、黄酮及萜类物质同步提取工艺优化 被引量:10
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作者 李欣宇 贾秀稳 +4 位作者 杨林 刘汉青 李雪璐 谢颂洋 张立华 《湖北农业科学》 2017年第15期2926-2930,共5页
为更有效地利用石榴(Punica granaum L.)花中多种活性成分,以石榴花为原料,利用乙醇溶剂浸提法,在单因素试验的基础上选择提取温度、提取时间、液固比、乙醇体积分数4个因素进行正交试验,优化了石榴花中3种活性物质同步提取工艺。结... 为更有效地利用石榴(Punica granaum L.)花中多种活性成分,以石榴花为原料,利用乙醇溶剂浸提法,在单因素试验的基础上选择提取温度、提取时间、液固比、乙醇体积分数4个因素进行正交试验,优化了石榴花中3种活性物质同步提取工艺。结果表明,影响石榴花中多酚、黄酮及萜类物质同步提取的因素大小顺序为提取温度〉提取时间〉乙醇体积分数〉液固比,其最优的工艺条件为60%乙醇溶液作为提取溶剂,液固比20∶1(m L∶g),85℃条件下提取2.0 h;在此条件下多酚、黄酮及三萜类物质的提取率分别是22.19%、6.53%、0.94%。石榴花中多酚、黄酮及萜类物质可以同步提取出来,可有效提高石榴花资源的利用率。 展开更多
关键词 石榴(Punica granaum l.) 多酚 黄酮 萜类 同步提取
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比色法测定刺槐花中总皂苷含量 被引量:3
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作者 计红芳 张令文 +3 位作者 杨铭铎 李斌 颜振敏 张帆 《湖北农业科学》 北大核心 2012年第17期3846-3849,共4页
建立了比色法测定刺槐(Robinia pseudoacacia L.)花提取物总皂苷含量的方法。以人参皂苷Rg1作为对照品,通过正交设计对香草醛-高氯酸比色法的试验条件进行优化,确定最佳比色条件为:60℃水浴挥干溶剂后,加入0.2 mL 7.0%香草醛-冰乙酸液和... 建立了比色法测定刺槐(Robinia pseudoacacia L.)花提取物总皂苷含量的方法。以人参皂苷Rg1作为对照品,通过正交设计对香草醛-高氯酸比色法的试验条件进行优化,确定最佳比色条件为:60℃水浴挥干溶剂后,加入0.2 mL 7.0%香草醛-冰乙酸液和1 mL高氯酸,于60℃水浴20 min,测定波长为545nm。样品在0~0.24 mg范围内呈良好的线性关系,回归方程为y=3.547 0x+0.010 9(R2=0.998 9),平均加样回收率为100.07%(RSD=2.10%,n=7),测得刺槐花提取物中总皂苷平均含量为2.177%。该方法简便、准确、重现性与稳定性较好,可用于测定刺槐花提取物总皂苷含量。 展开更多
关键词 刺槐(Robinia PSEUDOACACIA l.) 总皂苷 含量测定 比色法
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Characterization and Identification of a Novel Mutant fon(t) on Floral Organ Number and Floral Organ Identity in Rice 被引量:8
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作者 李云 徐培洲 +4 位作者 张红宇 彭海 张全芳 汪旭东 吴先军 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第8期730-737,共8页
The floral-organ-number mutant fon(t) was firstly discovered in the progeny of a cross between a diploid (Chunjiang 683) and a haploid (SARⅣ/-620-A) rice cultivar. The fon(t) mutant showed normal vegetative d... The floral-organ-number mutant fon(t) was firstly discovered in the progeny of a cross between a diploid (Chunjiang 683) and a haploid (SARⅣ/-620-A) rice cultivar. The fon(t) mutant showed normal vegetative development and produced normal inflorescence structures. Difference between the mutant and the wild type was observed when the stamen primordia began to form. The mature flowers offon(t) mutant showed open-hull phenotypes, which resulted in the exposure of stamens and stigmas. Normally, a single fon(t) floret consisted of six to nine stamens and one or two pistils. In addition, stamen/pistil-like structures and bulged tissues near ovaries were also observed in a few fon(t) florets. But homeotic transformation of lodicules into palea/lemma-like organs was observed almost in all the open-hull florets. The phenotypes offon(t) flowers also suggested thatfon(t) gene might affect flower organ identity in the inner whorls. Genetic analysis showed that thefon(t) mutant was controlled by a single recessive gene. 展开更多
关键词 flower organ identity flower organ number mutantfon(t) flower development
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Morphological,Anatomical and Genetic Analysis for a Rice Mutant with Abnormal Hull 被引量:6
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作者 张全芳 徐建第 +3 位作者 李云 徐培州 张红宇 吴先军 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第6期519-526,共8页
A mutant with abnormal hull was first discovered from a twin-seedling strain W2555 in rice (Oryza sativa L.). The mutant had sparse branches and decreased number of florets from the base to the peak. Frequently, the... A mutant with abnormal hull was first discovered from a twin-seedling strain W2555 in rice (Oryza sativa L.). The mutant had sparse branches and decreased number of florets from the base to the peak. Frequently, the florets at the top of the panicle did not develop completely. The underdeveloped florets often showed slender and white in their life cycle. Genetic analysis indicated that the mutant traits were controlled by a single recessive gene (temporarily designated as ah). ah gene controlled the development of inflorescence meristem and the flower organ. The florets of mutant showed degenerated lemma and palea. Stamens and lodicules were homeoticly transformed into pistils and palea/lemma-like structures, respectively. It seemed that ah mutant phenotypes of the homeotic conversions in lodicules and stamens were very similar to that of the B loss-of-function spwl gene reported previously in rice. 展开更多
关键词 Rice (Oryza sativa l.) ah mutant floral organ anatomical structure genetic analysis
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Evolution of Main Characteristics of the Registered Cotton Cultivars in National Cotton Regional Trials in Yangtze River Valley 被引量:1
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作者 许乃银 李健 《Agricultural Science & Technology》 CAS 2014年第6期964-969,共6页
[Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification ... [Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification and comprehensive evaluation index were set up based on national cotton registration standard. GGE biplot method was adopted to analyze the correlation of major breeding target characters of 53 national registered cotton varieties in cotton regional trials in YaRV during 1981-2012. According to the shift of check cultivars in cotton regional trials in the past, the cotton regional trial practice since 1981 was divided into five periods. The dynamic of cultivar type's proportion and the evaluation index scores was analyzed across the five periods. [Result] There existed intricate interrelationship among cotton breeding target traits, which constrained it necessary to construct indices for com- prehensive evaluation of cotton varieties. The dynamic of cultivar types in the five periods indicated that type II varieties emerged since Simian 3 period and then its proportion decreased gradually; type Ⅲ varieties maintained a certain proportion in each period and kept on the rise overall; type Ⅳvarieties occupied the majority pro- portion of registered cultivars before 1993, but a minor proportion since Simian 3 period. On the other side, the change trend of the evaluation index demonstrated that the varieties registered before 2003 did not pass the qualified line at present. The peak scores appeared in the varieties registered during 2004-2008. The scores of the varieties registered after 2009 were only slightly over the qualified line. [Conclusion] More attention should be paid to the improvement and evaluation of micronaire, so as to guide the simultaneous development of high yielding and fiber quality in cotton breeding and registration procedure in YaRV. 展开更多
关键词 Cotton (Gossypium hirsutum l.) GGE biplot Variety type classification The Yangtze River Valley (YaRV)
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国槐花与刺槐花中黄酮的质量差异比较 被引量:2
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作者 付凤萍 高欣悦 +3 位作者 余佳 吴建英 余琳 陈红英 《湖北农业科学》 2022年第7期108-112,共5页
国槐(Sophora japonica L.)花和刺槐(Robinia pseudoacacia L.)花为近缘品种,均有药食两用价值,并富含黄酮类物质。采用药典中槐花的标准来探讨两者黄酮差异,以利于经销和临床上区分。试验以芦丁为对照,对2种槐花进行薄层色谱定性比较... 国槐(Sophora japonica L.)花和刺槐(Robinia pseudoacacia L.)花为近缘品种,均有药食两用价值,并富含黄酮类物质。采用药典中槐花的标准来探讨两者黄酮差异,以利于经销和临床上区分。试验以芦丁为对照,对2种槐花进行薄层色谱定性比较、比色法测定总黄酮含量及高效液相色谱法测定芦丁含量。国槐花与刺槐花的色谱图差异非常明显,国槐花中芦丁为主要化学成分,而刺槐花中有不同于芦丁的主成分;含量测定结果显示,国槐花中总黄酮和芦丁含量分别约为刺槐花含量的1.5倍和5~10倍;2种槐花总黄酮含量均大于8%;同种槐花不同产地的总黄酮呈现量的差异;国槐花炮制后,按照生槐花、炒槐花、槐花炭的顺序,总黄酮和芦丁含量均逐渐减低。2种槐花色谱差异显著,可用于二者的定性鉴别;刺槐花中总黄酮和芦丁的含量均明显低于国槐花;不同产地同种槐花呈现量上差异,色谱图差异不大;槐花炮制后黄酮成分发生一定的改变;试验结果可用于两者的黄酮差异比较。 展开更多
关键词 国槐(Sophora japonica l.) 刺槐(Robinia pseudoacacia l.) 总黄酮 薄层色谱法 比色法 高效液相色谱法
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Comparison of Biochemical Substances in the Fiber Development of Different Cotton Varieties
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作者 葛杰 罗淑萍 +2 位作者 马德英 羌松 姚正培 《Agricultural Science & Technology》 CAS 2015年第3期439-441,共3页
Zhongmian 42 and Xinluzao 36 were used as raw materials to determine the contents of soluble sugar and protein, as well as dynamic changes of enzyme activities after flowering during cotton fiber growth. The results s... Zhongmian 42 and Xinluzao 36 were used as raw materials to determine the contents of soluble sugar and protein, as well as dynamic changes of enzyme activities after flowering during cotton fiber growth. The results showed that contents of soluble protein in the two species sharply declined 7 to 21 days after flowering, as the soluble sugar in Zhongmian 42 leveling off after 21 days of flowering while the soluble sugar in Xinluzao 36 dropped notably after 21 days of flowering before remaining stable after seven days later. The soluble sugar decreased 7 to 14 days after flowering before sharply rising to the maximum seven days later, and then began to decline quickly. The soluble sugar was the minimum after 35 days of flowering and then remaining stable. Peroxidase activity generally increased. Indole-3- acetic acid oxidase activities were low at 7 days after flowering. IAAO activity reached to the peaks on the 14th and 28th day after flowering. IAAO activity of two varieties decreased with the same trend 35 days after flowering. 展开更多
关键词 Cotton (Gossypium hirsutum l.) Cotton fiber Soluble sugar Soluble protein PEROXIDASE Indole-3-acetic acid oxidase
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不同工艺提取蝶豆花香料及在卷烟中的应用 被引量:5
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作者 韩航航 徐竖 +2 位作者 白冰 刘煜宇 包秀萍 《湖北农业科学》 2019年第10期109-113,117,共6页
采用超声波提取法、超临界CO2萃取法和分子蒸馏法3种方法制备了蝶豆(Clitoria ternatea L.)花提取物并用GC/MS法分析了蝶豆花提取物中的挥发性成分,并进行了卷烟加香试验。结果表明,超临界CO2萃取的净油鉴定出的挥发性成分最多,相对含... 采用超声波提取法、超临界CO2萃取法和分子蒸馏法3种方法制备了蝶豆(Clitoria ternatea L.)花提取物并用GC/MS法分析了蝶豆花提取物中的挥发性成分,并进行了卷烟加香试验。结果表明,超临界CO2萃取的净油鉴定出的挥发性成分最多,相对含量也最高,其中大部分是构成卷烟香味的重要物质;超声波提取法从蝶豆花中提取出对烟草有贡献的潜香物质效果一般,提取物中挥发性成分不如其他方法丰富,卷烟上的应用效果也不如其他方法好;蝶豆花提取物可用于卷烟加香,可以提质增香、增加吃味、降低刺激性、改善余味、提升卷烟抽吸品质。 展开更多
关键词 蝶豆(Clitoria ternatea l.) 超临界CO2萃取 超声波提取 分子蒸馏 卷烟 香料
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松花葛根片解酒作用的量效关系和机制研究 被引量:1
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作者 王升贵 黄静 +4 位作者 王张 黄雷雷 石璐 李春雨 杨永茂 《中药药理与临床》 CAS CSCD 北大核心 2014年第4期110-113,共4页
目的:研究松花葛根片解酒作用的量效关系和机制。方法:观察松花葛根片对醉酒模型小鼠及其血中乙醇含量、乙醇致小鼠自发活动增加的影响。结果:在1.67、0.83 g/kg的剂量下,松花葛根片具有减少醉倒率、延长睡眠潜伏期、缩短睡眠维持期的... 目的:研究松花葛根片解酒作用的量效关系和机制。方法:观察松花葛根片对醉酒模型小鼠及其血中乙醇含量、乙醇致小鼠自发活动增加的影响。结果:在1.67、0.83 g/kg的剂量下,松花葛根片具有减少醉倒率、延长睡眠潜伏期、缩短睡眠维持期的解酒作用,能减弱ALT活性和抑制乙醇致小鼠自发活动增加,可能与减少乙醇吸收、加速代谢,最终降低血中乙醇含量有关。在10.00、5.00、2.50g/kg的剂量下,葛根提取物亦具有解酒作用,并能抑制乙醇致小鼠自发活动增加。在松花葛根片处方剂量下(0.32、0.16、0.08g/kg),L-半胱氨酸盐酸盐没有解酒作用。结论:松花葛根片和葛根提取物的解酒作用均呈现量效关系,其作用机制可能与减少乙醇吸收和加速代谢、降低乙醇的神经兴奋性、减弱ALT活性有关。 展开更多
关键词 葛根片 葛根 l-半胱氨酸盐酸盐 酒精中毒 乙醇 量效关系 自发活动
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Substrate availability regulates the suppressive effects of Canada goldenrod invasion on soil respiration 被引量:1
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作者 Zhiyuan Hu Jiaqi Zhang +7 位作者 Yizhou Du Kangwei Shi Guangqian Ren Babar Iqbal Zhicong Dai Jian Li Guanlin Li Daolin Du 《Journal of Plant Ecology》 SCIE CSCD 2022年第3期509-523,共15页
Invasive alien plants not only decrease riparian vegetation diversity but also alter wetland ecosystem carbon processes,especially when they displace the original vegetation.Invasive Canada goldenrod(Solidago canadens... Invasive alien plants not only decrease riparian vegetation diversity but also alter wetland ecosystem carbon processes,especially when they displace the original vegetation.Invasive Canada goldenrod(Solidago canadensis L.)has colonized large areas of disturbed and undisturbed land in southeastern China,yet little is known regarding how it affects soil carbon cycling.To explore the response patterns of soil respiration following S.canadensis invasion and their driving mechanisms,an observational field study and a greenhouse experiment simulating invasion were performed.In the field study,soil respiration was measured weekly from 21th July 2018 to 15th December 2018.In the greenhouse experiment,soil,autotrophic and heterotrophic respiration were measured every 1st and 15th of the month from 15th July 2019 to 15th December 2019.Soil,autotrophic and heterotrophic respiration were measured using a closed-chamber system with the deep gauze collar root exclusion method.Solidago canadensis invasion appeared to decrease the total soil CO_(2) emissions in both the field study and the greenhouse experiment.The suppressive effects on soil respiration may be attributed to S.canadensis invasion-induced alterations in the quality and quantity of available soil substrate,suggesting that S.canadensis invasion may impact soil carbon cycling via plant-released substrates and by competing for the soil available substrate with native plant and/or soil microbes.These results have substantial implications for estimations of the effects of invasive plants on belowground carbon dynamics and their contribution to the warming world. 展开更多
关键词 Solidago canadensis l.Phragmites australis(Cav.)Trin.ex Steud soil CO_(2) emission oil substrate availability competition carbon cycling
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