[Objectives] To establish a method for the determination of tannin in Sanguisorba officinalis L.,and provide guarantee for further control over quality of semi-finished tannin products from Sanguisorba officinalis L. ...[Objectives] To establish a method for the determination of tannin in Sanguisorba officinalis L.,and provide guarantee for further control over quality of semi-finished tannin products from Sanguisorba officinalis L. [Methods] With gallic acid as reference substance,the UV method was used to determine the content of tannin in Sanguisorba officinalis L.,and the methodological examination was done. [Results]With gallic acid as standard,when the concentration of tannin in Sanguisorba officinalis L. was 1. 02-10. 14 μg·m L^(-1),there was a good linear relationship with absorbance,the precision,reproducibility and recovery were in line with the requirements,and the absorbance value was stable within 40 min after coloration. [Conclusions] The method was simple,rapid and accurate in determining the content of tannin in Sanguisorba officinalis L.展开更多
The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, ...The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, could be one way of building resilience in vulnerable farming households. The aim of this study was to determine the secondary metabolite and mineral composition of noni juice obtained by fermenting the fruit of Morinda citrifolia L. Fruits were collected in August 2022 from the local field in Thiès region, West of Senegal. Extraction yields were determined and the secondary metabolites were determined using conventional analytical methods. Calcium, magnesium, iron, sodium and potassium were determined by atomic absorption spectrophotometer coupled with a CCD detector. The results show that an average fruit mass (503.2 ± 110.96 g) consists of 171.44 ± 50.01 g pulp and 34.06 ± 10.35 g seeds. The traditional extraction yield of noni juice is 16.46% after three weeks of fermentation. The contents of total polyphenols, flavonoids and tannins obtained in noni are 608.97 ± 4.53 mg EAG/100mL, 7.78 ± 0.01 mg EQ/100mL and 0.191 ± 0.01 mg EC/100mL respectively. The ethanol content of noni varies from 3.57 to 5.23 mL/100mL during extraction. Noni has a high calcium content with a concentration of 383.79 ± 33.23 mg/L. This is followed by a good concentration of magnesium, potassium and sodium, at 278.47 ± 26.30, 187.43 ± 10.7 and 155.95 ± 28.66 mg/L respectively. Noni also has an iron content of 202.15 ± 0.05 mg/L.展开更多
Phytochemical screening and assay of secondary metabolites, crude extracts with distilled water, aqueous methanol, aqueous acetone and aqueous ethanol of leaves and seeds of Senna occidentalis L. were studied in this ...Phytochemical screening and assay of secondary metabolites, crude extracts with distilled water, aqueous methanol, aqueous acetone and aqueous ethanol of leaves and seeds of Senna occidentalis L. were studied in this work. The aim was to verify the distribution of secondary metabolites according to S. occidentalis organs. Four leaf samples from four different localities (South, East, West and Central Senegal) and a mixed sample of seeds were used. Functional molecules such as polyphenols, flavonoids and tannins were then assessed in the leaves and seeds using various standard methods. The results show that Senna occidentalis L. leaf and seed samples display an identical and homogeneous profile, regardless of locality. They contain secondary metabolites and the polyphenol content of extracts from southern, eastern, western and central leaves is: 0.620 - 0.539 - 0.811 - 0.573 g GAE/100 g DM;flavonoids: 0.064 - 0.074 - 0.130 - 0.101 g CE/100 g DM and tannins: 0.326 - 0.264 - 0.269 - 0.494 g TAE/100 g DM. The efficacy of S. occidentalis L. infusions in therapy is thus justified by the presence of these metabolites, whose biological properties are well known. It is then possible to explore isolation of active principles of Senna occidentalis L. leaves and even seeds for producing medicines.展开更多
目的:建立高效液相色谱法(HPLC)同时测定毛连菜中咖啡酸和木犀草苷含量的方法。方法:采用Waters Sun Fire C_(18)色谱柱(250 mm×4.6 mm 5μm),流动相为乙腈-0.1%磷酸溶液梯度洗脱,检测波长323 nm,流速1.0 ml·min^(-1),进样量1...目的:建立高效液相色谱法(HPLC)同时测定毛连菜中咖啡酸和木犀草苷含量的方法。方法:采用Waters Sun Fire C_(18)色谱柱(250 mm×4.6 mm 5μm),流动相为乙腈-0.1%磷酸溶液梯度洗脱,检测波长323 nm,流速1.0 ml·min^(-1),进样量10μl,柱温30℃。结果:咖啡酸和木犀草苷的色谱峰可较好地分离,咖啡酸浓度在1.014～40.560μg·ml^(-1)(r=0.999 5)、木犀草苷浓度在2.006～80.240μg·ml^(-1)(r=1000 0)范围内与峰面积呈良好的线性关系;平均加样回收率分别为92.84%,92.62%,RSD分别为2.0%,1.93%(n=6)。结论:该方法准确性、重复性、专属性较好,可用于毛连菜的质量控制。展开更多
The information related with results of a project on germplasm characterization of three Lotus species, important as forage plants, curried out in five environments of the central-southern zone of Chile, was reviewed....The information related with results of a project on germplasm characterization of three Lotus species, important as forage plants, curried out in five environments of the central-southern zone of Chile, was reviewed. An evaluation was made of 12 L. corniculatus cultivars introduced from different parts of the world, as well as germplasm naturalized in Chile, being 11 accessions of L. tenuis and 21 accessions ofL. uliginosus collected from 33°S to 38°S and 38°S to 45°S, respectively. Phenological, morphological, and agronomical characters were evaluated. Nitrogen fixation in field conditions and concentrations of condensed tannins in herbage were also included. It is concluded that there are cultivars of high value in L. corniculatus introduced germplasm which can be recommended for different environments of the central zone of Chile, as well as for local species breeding programmes. Information obtained from L. tenuis and L. uliginosus accessions shows genetic variability within both species. L. tenuis germplasm is adapted to clay, medium-textured, or sandy soils with water restrictions and phosphorous deficiency. Therefore, characterized accessions could be used to breed cultivars for low input production systems in constrained environments. L. uliginosus accessions include genotypes collected in sites with acid soils and variable tannin content in plant tissues that may be promissory genetic materials for breeding programmes with the objective of producing cultivars for animal production systems for the wet and acid soils in the southern zone of the country.展开更多
文摘[Objectives] To establish a method for the determination of tannin in Sanguisorba officinalis L.,and provide guarantee for further control over quality of semi-finished tannin products from Sanguisorba officinalis L. [Methods] With gallic acid as reference substance,the UV method was used to determine the content of tannin in Sanguisorba officinalis L.,and the methodological examination was done. [Results]With gallic acid as standard,when the concentration of tannin in Sanguisorba officinalis L. was 1. 02-10. 14 μg·m L^(-1),there was a good linear relationship with absorbance,the precision,reproducibility and recovery were in line with the requirements,and the absorbance value was stable within 40 min after coloration. [Conclusions] The method was simple,rapid and accurate in determining the content of tannin in Sanguisorba officinalis L.
文摘The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, could be one way of building resilience in vulnerable farming households. The aim of this study was to determine the secondary metabolite and mineral composition of noni juice obtained by fermenting the fruit of Morinda citrifolia L. Fruits were collected in August 2022 from the local field in Thiès region, West of Senegal. Extraction yields were determined and the secondary metabolites were determined using conventional analytical methods. Calcium, magnesium, iron, sodium and potassium were determined by atomic absorption spectrophotometer coupled with a CCD detector. The results show that an average fruit mass (503.2 ± 110.96 g) consists of 171.44 ± 50.01 g pulp and 34.06 ± 10.35 g seeds. The traditional extraction yield of noni juice is 16.46% after three weeks of fermentation. The contents of total polyphenols, flavonoids and tannins obtained in noni are 608.97 ± 4.53 mg EAG/100mL, 7.78 ± 0.01 mg EQ/100mL and 0.191 ± 0.01 mg EC/100mL respectively. The ethanol content of noni varies from 3.57 to 5.23 mL/100mL during extraction. Noni has a high calcium content with a concentration of 383.79 ± 33.23 mg/L. This is followed by a good concentration of magnesium, potassium and sodium, at 278.47 ± 26.30, 187.43 ± 10.7 and 155.95 ± 28.66 mg/L respectively. Noni also has an iron content of 202.15 ± 0.05 mg/L.
文摘Phytochemical screening and assay of secondary metabolites, crude extracts with distilled water, aqueous methanol, aqueous acetone and aqueous ethanol of leaves and seeds of Senna occidentalis L. were studied in this work. The aim was to verify the distribution of secondary metabolites according to S. occidentalis organs. Four leaf samples from four different localities (South, East, West and Central Senegal) and a mixed sample of seeds were used. Functional molecules such as polyphenols, flavonoids and tannins were then assessed in the leaves and seeds using various standard methods. The results show that Senna occidentalis L. leaf and seed samples display an identical and homogeneous profile, regardless of locality. They contain secondary metabolites and the polyphenol content of extracts from southern, eastern, western and central leaves is: 0.620 - 0.539 - 0.811 - 0.573 g GAE/100 g DM;flavonoids: 0.064 - 0.074 - 0.130 - 0.101 g CE/100 g DM and tannins: 0.326 - 0.264 - 0.269 - 0.494 g TAE/100 g DM. The efficacy of S. occidentalis L. infusions in therapy is thus justified by the presence of these metabolites, whose biological properties are well known. It is then possible to explore isolation of active principles of Senna occidentalis L. leaves and even seeds for producing medicines.
文摘目的:建立高效液相色谱法(HPLC)同时测定毛连菜中咖啡酸和木犀草苷含量的方法。方法:采用Waters Sun Fire C_(18)色谱柱(250 mm×4.6 mm 5μm),流动相为乙腈-0.1%磷酸溶液梯度洗脱,检测波长323 nm,流速1.0 ml·min^(-1),进样量10μl,柱温30℃。结果:咖啡酸和木犀草苷的色谱峰可较好地分离,咖啡酸浓度在1.014～40.560μg·ml^(-1)(r=0.999 5)、木犀草苷浓度在2.006～80.240μg·ml^(-1)(r=1000 0)范围内与峰面积呈良好的线性关系;平均加样回收率分别为92.84%,92.62%,RSD分别为2.0%,1.93%(n=6)。结论:该方法准确性、重复性、专属性较好,可用于毛连菜的质量控制。
文摘The information related with results of a project on germplasm characterization of three Lotus species, important as forage plants, curried out in five environments of the central-southern zone of Chile, was reviewed. An evaluation was made of 12 L. corniculatus cultivars introduced from different parts of the world, as well as germplasm naturalized in Chile, being 11 accessions of L. tenuis and 21 accessions ofL. uliginosus collected from 33°S to 38°S and 38°S to 45°S, respectively. Phenological, morphological, and agronomical characters were evaluated. Nitrogen fixation in field conditions and concentrations of condensed tannins in herbage were also included. It is concluded that there are cultivars of high value in L. corniculatus introduced germplasm which can be recommended for different environments of the central zone of Chile, as well as for local species breeding programmes. Information obtained from L. tenuis and L. uliginosus accessions shows genetic variability within both species. L. tenuis germplasm is adapted to clay, medium-textured, or sandy soils with water restrictions and phosphorous deficiency. Therefore, characterized accessions could be used to breed cultivars for low input production systems in constrained environments. L. uliginosus accessions include genotypes collected in sites with acid soils and variable tannin content in plant tissues that may be promissory genetic materials for breeding programmes with the objective of producing cultivars for animal production systems for the wet and acid soils in the southern zone of the country.
