L形冷弯薄壁型钢组合钢管混凝土柱轴压性能

为解决异形钢管混凝土柱阴角对其承载力的影响,提出了用1个冷弯薄壁方钢管和2个U形钢管焊接成L形钢管,内填充混凝土形成L形冷弯薄壁型钢组合钢管混凝土柱形式。设计了5组共10根试件的轴压试验,并开展了有限元参数分析,研究了钢管厚度、U形管外伸长度和钢材强度等参数对构件承载力和延性等力学性能的影响。结果表明:该类试件的主要破坏形态为中上部局部鼓曲破坏,适量增大U形管外伸长度可以提高承载力,但增大到一定程度之后易发生弯扭破坏;承载力和延性随着钢管厚度和钢材强度增大而增加;混凝土强度对构件的初始刚度和峰值荷载的影响都很小,但对曲线下降段的影响较大;试件端部和中部截面阴角处的混凝土应力值比各边中部更大,说明采用U形钢管与方钢管组合的方式改善了阴角处钢管对混凝土约束普遍较弱的问题。基于“统一理论”,给出了两种承载力建议计算公式,与试验结果吻合较好,两种计算方法在0.44~1.94的约束效应系数范围内具有良好的适用性。

Identification and Transcriptional Regulation of CAMTA Genes in Liriodendron chinense

This study explores CAMTA genes in the rare and endangered Chinese plant species,Liriodendron chinense.Despite the completion of whole-genome sequencing,the roles of CAMTA genes in calcium regulation and stress responses in this species remain largely unexplored.Within the L.chinense genome,we identified two CAMTA genes,Lchi09764 and Lchi222536,characterized by four functional domains:CG-1,TIG,ANK repeats,and IQ motifs.Our analyses,including phylogenetic investigations,cis-regulatory element analyses,and chromosomal location studies,aim to elucidate the defining features of CAMTA genes in L.chinense.Applying Weighted Gene Co-Expression Network Analysis(WGCNA),we explored the impact of CAMTA genes on different organs and their regulation under abiotic stress conditions.The identification of significant gene modules and the prediction of promoter binding sites revealed co-expressed genes associated with CAMTA transcription factors.In summary,this study provides initial insights into CAMTA genes in L.chinense,laying the groundwork for future research on their evolution and biological roles.This knowledge enhancement contributes to a better understanding of plant responses to environmental stress—an essential aspect of plant biology.

The flavonoids from Pentorum chinense Pursh.mediates ferroptosis to alleviate sorafenib-induced liver injury in BRL-3A cells

Background:Drug-induced liver damage is a severe medical issue that affects people all over the world.Sorafenib has some side effects that cause liver injury.A dietary medicinal plant called Penthorum chinense Pursh.(PCP)has hepatoprotective properties.There are currently few reports on PCP’s protective impact and mechanism against sorafenib-induced liver injury.Methods:To create a liver injury model,sorafenib was administered to BRL-3A cells.Cell viability assays,immunofluorescence tests,Western blotting,real-time quantitative PCR,and high-content imaging systems were utilized to examine PCP’s effect and mechanism.Results:In this study,PCP treatment mitigated the liver damage caused by sorafenib by enhancing cell survival,lowering lipid reactive oxygen species and malondialdehyde levels,and elevating glutathione levels.In addition,PCP can enhance the protein expression of cystine/glutamate transporter xCT and glutathione peroxidase 4,reduce iron content and alleviate mitochondrial toxicity.Further mechanism studies revealed that PCP inhibited ferroptosis by promoting the production of nuclear factor E2-related factor 2 nuclear translocation and subsequently affecting target genes(HO-1 and NQO1).Conclusion:Together,PCP regulates the nuclear factor E2-related factor 2 pathway,which helps to lessen ferroptosis brought on by sorafenib.

Optimization of Extraction Process for Total Flavonoids from Penthorum chinense Pursh and Comparison of Their Contents from Different Parts

[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh.

Chemical composition and pharmacological activities of the Phellodendron chinense Schneid

Phellodendron chinense Schneid(P.chinense),as a traditional Chinese medicine,is commonly used in clinical practice.It has the effects of drying dampness,clearing heat,detoxifying,purging fire,and reducing swelling.Its main chemical components are flavonoids and alkaloids,which have various pharmacological effects such as lowering blood sugar,lowering blood pressure and immunosuppression.With the continuous development of modern science and technology,the research on P.chinense Schneid has increased.This study reviews the chemical components and pharmacological effects of P.chinense Schneid,and provides reference for its further research and development.

甘蓝型油菜BnaSLY1基因进化分析及功能研究

赤霉素调控植物表皮细胞增长、茎叶伸长以及株型建成。拟南芥SLY1属于F-box蛋白,它通过靶向泛素化赤霉素信号转导路径的负向调控因子——DELLA蛋白来影响植物生长发育,然而油菜中BnaSLY1的基因功能尚未揭示。本研究对BnaSLY1进行了表达特征和进化树分析,利用CRISPR/Cas9技术创制了BnaSLY1不同拷贝数的突变体,结合RNA-Seq技术对BnaSLY1的生物学功能及其对油菜生长发育的影响进行了研究。结果表明,甘蓝型油菜Westar中有2个SLY1同源拷贝BnaA01.SLY1和BnaA06.SLY1,它们表达模式基本相同,为组成型表达基因,其蛋白定位在细胞核,且在不同的油菜品种及十字花科植物间序列保守。与对照相比,单突bnaa01sly1和bnaa06sly1开花时间推迟,株高显著降低,而双突bnasly1还表现出深绿色光叶表型,叶片厚度增加,开花期比单突进一步推迟,株高也进一步降低。RNA-Seq结果显示,双突与Westar之间的差异表达基因显著富集在生长素信号转导路径以及蜡质合成通路,多个开花时间相关基因表达也发生显著变化。本研究表明, BnaSLY1除影响植物株高和开花时间等生长发育进程,还影响表皮蜡质合成,为探索赤霉素信号转导路径在甘蓝型油菜生长发育中的重要作用奠定了理论基础。

缺血性脑卒中铁死亡特征基因NFE2L2的鉴定与验证

背景:铁死亡与缺血性脑卒中的发病密切相关,靶向铁死亡是一种治疗缺血性脑卒中有前景的方案,但具体调控靶点尚不明确。目的:通过生物信息学和机器学习方法筛选缺血性脑卒中铁死亡相关特征基因,并通过细胞实验进行验证,探讨铁死亡在缺血性脑卒中的作用。方法:基于GEO数据库和FerrDb数据库选取符合条件的缺血性脑卒中相关数据集和铁死亡表达数据集,通过t检验筛选铁死亡相关差异基因。对铁死亡相关差异基因进行GO功能富集分析与KEGG信号通路富集分析。通过PPI网络分析和机器学习筛选缺血性脑卒中铁死亡的特征基因,利用ROC分析和GSEA分析探究特征基因的准确性和生物功能。然后进行细胞实验,将HT22细胞分为对照组与缺血性脑卒中组,对照组不作任何干预,缺血性脑卒中组加入0.1 mol/L的H_(2)O_(2)干预24 h诱导细胞氧化应激和铁死亡,通过实时荧光定量RT-PCR和Western Blot验证铁死亡的发生和特征基因表达。结果与结论:(1)共获取45个铁死亡相关差异基因,GO和KEGG富集分析发现差异基因与氧化应激、自噬、铁死亡、脂肪细胞因子信号通路和线粒体代谢密切相关。(2)通过PPI网络中的MCODE插件和cytoHubba插件与机器学习中的LASSO算法和SVM-RFE算法共鉴定出1个铁死亡特征基因核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,NFE2L2)。(3)对NFE2L2进行ROC曲线分析,发现在训练集和验证集中构建的诊断预测模型具有良好的准确性与特异性;对NFE2L2进行GSEA分析,发现特征基因通过免疫、炎症反应、氨基酸代谢及神经因子调控等方面参与缺血性脑卒中发病机制的调控。(4)细胞实验的RT-PCR和Western Blot分析表明,与对照组对比,缺血性脑卒中组中的酰基辅酶A合成酶长链家族成员4 mRNA和蛋白表达水平显著增高(P<0.05),谷胱甘肽过氧化物酶4 mRNA和蛋白表达水平显著降低(P<0.05);与对照组对比,缺血性脑卒中组中特征基因NFE2L2 mRNA和蛋白表达水平显著增高(P<0.05)。(5)上述结果证实,缺血性脑卒中与铁死亡密切相关,靶向特征基因NFE2L2可以为研究和治疗缺血性脑卒中提供一定的思路与方向。

基于整车关键尺寸L114的平台架构研究

为了研究最优的整车平台架构策略,本文详细阐述平台架构的概念,定义了架构关键尺寸L114,列举国内某车企基于关键尺寸L114一致的平台拓展尺寸链,并梳理该平台架构乘员舱架构件具体的策略,通过对比分别采取平台车型关键尺寸L113一致与平台车型关键尺寸L114一致时的平台通用化率,发现2种策略的平台通用化率基本一致。同时结合整车安全碰撞策略进行分析,结果表明,若采用关键尺寸L114更具优势,该策略下同平台车型仅需满足高车型的碰撞策略,即可同步满足其他车型的侧碰要求,有效降低平台开发成本。

Effects of P301L-TAU on post-translational modifications of microtubules in human iPSC-derived cortical neurons and TAU transgenic mice

TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the axon.TAU is missorted and aggregated in an array of diseases known as tauopathies.Microtubules are essential for neuronal function and regulated via a complex set of post-translational modifications,changes of which affect microtubule stability and dynamics,microtubule interaction with other proteins and cellular structures,and mediate recruitment of microtubule-severing enzymes.As impairment of microtubule dynamics causes neuronal dysfunction,we hypothesize cognitive impairment in human disease to be impacted by impairment of microtubule dynamics.We therefore aimed to study the effects of a disease-causing mutation of TAU(P301L)on the levels and localization of microtubule post-translational modifications indicative of microtubule stability and dynamics,to assess whether P301L-TAU causes stability-changing modifications to microtubules.To investigate TAU localization,phosphorylation,and effects on tubulin post-translational modifications,we expressed wild-type or P301L-TAU in human MAPT-KO induced pluripotent stem cell-derived neurons(i Neurons)and studied TAU in neurons in the hippocampus of mice transgenic for human P301L-TAU(p R5 mice).Human neurons expressing the longest TAU isoform(2N4R)with the P301L mutation showed increased TAU phosphorylation at the AT8,but not the p-Ser-262 epitope,and increased polyglutamylation and acetylation of microtubules compared with endogenous TAU-expressing neurons.P301L-TAU showed pronounced somatodendritic presence,but also successful axonal enrichment and a similar axodendritic distribution comparable to exogenously expressed 2N4R-wildtype-TAU.P301L-TAU-expressing hippocampal neurons in transgenic mice showed prominent missorting and tauopathy-typical AT8-phosphorylation of TAU and increased polyglutamylation,but reduced acetylation,of microtubules compared with non-transgenic littermates.In sum,P301L-TAU results in changes in microtubule PTMs,suggestive of impairment of microtubule stability.This is accompanied by missorting and aggregation of TAU in mice but not in i Neurons.Microtubule PTMs/impairment may be of key importance in tauopathies.

Loss-of-function mutations of microRNA-142-3p promote ASH1L expression to induce immune evasion and hepatocellular carcinoma progression

BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated mortality.Efficient therapeutic targets are beneficial to treat and predict the disease.Currently,the exact molecular mechanisms leading to the progression of HCC are still unclear.Research has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor tissues.In this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between them.AIM To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC patients.METHODS In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC patients.Furthermore,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,etc.The statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank test.RESULTS In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival rate.ASH1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated region.Furthermore,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via ASH1L.For the exploration mechanism,we found ASH1L may promote an immunosuppressive microenvironment in HCC and ASH1L affects the expression of the cell junction protein zonula occludens-1,which is potentially relevant to the immune system.CONCLUSION Loss function of microRNA-142-3p induces cancer progression and immune evasion through upregulation of ASH1L in HCC.Both microRNA-142-3p and ASH1L can feature as new biomarker for HCC in the future.

载富血小板血浆水凝胶对L929细胞氧化损伤的保护作用

背景:在慢性创面愈合过程中,活性氧的过量生成可能会损害L929成纤维细胞的功能,从而延缓创面修复,因此保护成纤维细胞免受氧化应激的影响对于促进创面愈合非常重要。目的:评估羧甲基壳聚糖-氧化硫酸软骨素/富血小板血浆(CMC-OCS/PRP)水凝胶对H2O2刺激下L929细胞的保护作用。方法:制备CMC-OCS/PRP水凝胶,表征水凝胶的微观形貌、降解性能、清除H2O2与羟基自由基的能力及生物相容性。取生长状态良好的L929细胞,分5组培养:对照组常规培养,H2O2组加入H2O2,CMC-OCS组加入羧甲基壳聚糖-氧化硫酸软骨素水凝胶浸提液+H2O2,PRP组加入富血小板血浆凝胶浸提液+H2O2,CMC-OCS/PRP组加入羧甲基壳聚糖-氧化硫酸软骨素/富血小板血浆水凝胶浸提液处理+H2O2,每组先加入水凝胶浸提液处理6 h,然后再加入H2O2处理24 h。培养结束后,检测细胞活性氧及丙二醛水平,细胞凋亡、胶原纤维Ⅰ蛋白表达。在加入H2O2的情况下,将上述水凝胶浸提液分别与L929成纤维细胞直接或间接共培养36 h,通过划痕实验、Transwell小室实验检测细胞的迁移能力。结果与结论:①CMC-OCS/PRP水凝胶具有均匀相互关联的多孔结构及良好的降解能力,体外可有效清除H2O2与羟基自由基,并且具有良好的生物相容性;②与对照组比较,H2O2组细胞凋亡率、细胞活性氧与丙二醛水平升高(P<0.05),细胞铺展面积减少(P<0.05),胶原纤维Ⅰ蛋白表达无明显变化(P>0.05);与H2O2组比较,CMC-OCS组、CMC-OCS/PRP组细胞活性氧水平降低(P<0.05),PRP组、CMC-OCS组、CMCOCS/PRP组丙二醛水平降低(P<0.05)、细胞铺展面积增加(P<0.05),CMC-OCS/PRP组细胞凋亡率降低(P<0.05),PRP组、CMC-OCS组、CMCOCS/PRP组胶原纤维Ⅰ蛋白表达增加(P<0.05);③与对照组比较,H2O2组细胞迁移数量减少(P<0.05),迁移面积无明显变化(P>0.05);与H2O2组比较,PRP组、CMC-OCS组、CMC-OCS/PRP组细胞迁移数量、迁移面积均增加(P<0.05),并且以CMC-OCS/PRP组增加最显著;④在氧化应激条件下,CMC-OCS/PRP水凝胶可改善成纤维细胞的迁移能力,抗细胞凋亡并保护细胞伸展功能。

Recombinant chitinase-3-like protein 1 alleviates learning and memory impairments via M2 microglia polarization in postoperative cognitive dysfunction mice

Postoperative cognitive dysfunction is a seve re complication of the central nervous system that occurs after anesthesia and surgery,and has received attention for its high incidence and effect on the quality of life of patients.To date,there are no viable treatment options for postoperative cognitive dysfunction.The identification of postoperative cognitive dysfunction hub genes could provide new research directions and therapeutic targets for future research.To identify the signaling mechanisms contributing to postoperative cognitive dysfunction,we first conducted Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the Gene Expression Omnibus GSE95426 dataset,which consists of mRNAs and long non-coding RNAs differentially expressed in mouse hippocampus3 days after tibial fracture.The dataset was enriched in genes associated with the biological process"regulation of immune cells,"of which Chill was identified as a hub gene.Therefore,we investigated the contribution of chitinase-3-like protein 1 protein expression changes to postoperative cognitive dysfunction in the mouse model of tibial fractu re surgery.Mice were intraperitoneally injected with vehicle or recombinant chitinase-3-like protein 124 hours post-surgery,and the injection groups were compared with untreated control mice for learning and memory capacities using the Y-maze and fear conditioning tests.In addition,protein expression levels of proinflammatory factors(interleukin-1βand inducible nitric oxide synthase),M2-type macrophage markers(CD206 and arginase-1),and cognition-related proteins(brain-derived neurotropic factor and phosphorylated NMDA receptor subunit NR2B)were measured in hippocampus by western blotting.Treatment with recombinant chitinase-3-like protein 1 prevented surgery-induced cognitive impairment,downregulated interleukin-1βand nducible nitric oxide synthase expression,and upregulated CD206,arginase-1,pNR2B,and brain-derived neurotropic factor expression compared with vehicle treatment.Intraperitoneal administration of the specific ERK inhibitor PD98059 diminished the effects of recombinant chitinase-3-like protein 1.Collectively,our findings suggest that recombinant chitinase-3-like protein 1 ameliorates surgery-induced cognitive decline by attenuating neuroinflammation via M2 microglial polarization in the hippocampus.Therefore,recombinant chitinase-3-like protein1 may have therapeutic potential fo r postoperative cognitive dysfunction.

Autism spectrum disorder:difficulties in diagnosis and microRNA biomarkers

We performed a PubMed search for microRNAs in autism spectrum disorder that could serve as diagnostic biomarkers in patients and selected 17 articles published from January 2008 to December 2023,of which 4 studies were performed with whole blood,4 with blood plasma,5 with blood serum,1 with serum neural cell adhesion molecule L1-captured extracellular vesicles,1 with blood cells,and 2 with peripheral blood mononuclear cells.Most of the studies involved children and the study cohorts were largely males.Many of the studies had performed microRNA sequencing or quantitative polymerase chain reaction assays to measure microRNA expression.Only five studies had used real-time polymerase chain reaction assay to validate microRNA expression in autism spectrum disorder subjects compared to controls.The microRNAs that were validated in these studies may be considered as potential candidate biomarkers for autism spectrum disorder and include miR-500a-5p,-197-5p,-424-5p,-664a-3p,-365a-3p,-619-5p,-664a-3p,-3135a,-328-3p,and-500a-5p in blood plasma and miR-151a-3p,-181b-5p,-320a,-328,-433,-489,-572,-663a,-101-3p,-106b-5p,-19b-3p,-195-5p,and-130a-3p in blood serum of children,and miR-15b-5p and-6126 in whole blood of adults.Several important limitations were identified in the studies reviewed,and need to be taken into account in future studies.Further studies are warranted with children and adults having different levels of autism spectrum disorder severity and consideration should be given to using animal models of autism spectrum disorder to investigate the effects of suppressing or overexpressing specific microRNAs as a novel therapy.

Establishment of Somatic Cell Clones in Thesium chinense Turcz and Its in vitro Rooting Technique

[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.

不同类型群落中濒危植物永瓣藤(Monimopetalum chinense)种群的空间分布格局

为揭示永瓣藤(Monimopetalum chinense)种群的分布格局特征及其成因,采用分形分析中的计盒维数对取自4个分布地的13个样方进行了研究。结果显示:(1)同一研究地不同群落中永瓣藤种群的分形特征差异明显,而不同研究地同一类型群落之间的差异不大,分析认为这与永瓣藤在群落中的生态地位及生境异质性有关。(2)永瓣藤种群计盒维数由高到低依次为杉木林(1.911±0.025)、杂木林(1.806±0.079)、马尾松林(1.692±0.054)和毛竹林(1.629±0.081),表明不同群落中其个体分布的非均匀性存在较大差异。(3)永瓣藤种群的拐点尺度由高到低依次为毛竹林(4.111m±0.484m)、马尾松林(3.778m±0.222m)、杂木林(2.857m)、杉木林(2.013m±0.117m),次序与计盒维数相反。(4)分析认为,不同群落中永瓣藤无性繁殖能力的不同对其种群格局的计盒维数以及拐点尺度有重要影响。

杀线虫真菌Simplicillium chinense菌株Snef5强毒和弱毒菌株的诱变筛选

杀线虫真菌Simplicillium chinense菌株Snef5分离自柞蚕茧蛹体内,其发酵液对多种线虫均具有较高的致死作用。为筛选对南方根结线虫强毒与弱毒的菌株,采用紫外照射、硫酸二乙酯处理及农杆菌介导的遗传转化等3种诱变方法对菌株Snef5进行诱变育种,结果表明:共筛选得到8株强毒菌株,3株弱毒菌株。复筛后获得毒力稳定的菌株共4株,包括强毒菌株UV-17和UV-18,弱毒菌株UV-15和T-25,以农杆菌介导的遗传转化法诱变处理效果最佳。诱变菌株UV-17、UV-18、UV-15和T-25的获得,为将来对杀线虫活性基因簇进行分析研究奠定了基础。

Saikosaponin q-1 from Bupleurum chinense

A new saikosaponin was isolated from Bupleurum chinense DC., and its structure was identified as 3β,16α,23,28,30_pentahydroxy_olean_11,13(18)_dien_3_O_β_D_glucopyranosyl(1→6)_[α_L_rhamnopyranosyl (1→4)]_β_D_glucopyranoside on the basis of chemical and spectral evidence, named as saikosaponin q_1. In addition, two known saikosaponins, 3″_O_acetyl_saikosaponin d and 3″_O_acetyl_saikosaponin b 2, were also isolated and identified from this plant for the first time.

中华蚊母树(Distylium chinense)幼苗对秋、冬季淹水的生长及生理响应

为阐明三峡库区岸生植物中华蚊母树(Distylium chinense)幼苗对水淹的耐淹机理,通过设置对照组(CK)、半淹组(PF)、全淹组(CF)3个水淹处理,模拟库区消落带秋、冬季淹水情况,研究中华蚊母树幼苗在不同水淹时间和深度下的生长及生理生化特性.结果表明,淹水显著影响中华蚊母树幼苗的生物量,水淹150 d后,PF组植株和CF组植株根、茎、叶生物量均呈显著降低趋势,同时受水淹影响,植株根冠比也显著降低;中华蚊母树幼苗在水淹前90 d 2组水淹处理植株叶片丙二醛(MDA)含量均与CK组植株差异不显著,水淹120 d后,中华蚊母树幼苗叶片MDA含量显著增加,且随着水淹深度的增加而逐渐升高;可溶性蛋白在各水淹处理中的变化有所差异,水淹前60 d,淹水并没有影响CF组植株可溶性蛋白含量,但从水淹90 d开始,CF组植株可溶性蛋白含量有所上升,而PF组植株可溶性蛋白含量在整个淹水期间均与CK组植株差异不显著;2组水淹处理植株脯氨酸在水淹前期均显著上升,但自水淹90 d后逐渐下降至CK组水平;与MDA对水淹的响应一致,水淹前期PF组和CF组植株抗氧化酶活性(超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶)均没有出现明显影响,但水淹后期2组水淹处理植株抗氧化酶活性均显著升高;可溶性糖含量在不同水淹处理下有所差异,PF组植株叶片可溶性糖含量在整个淹水期间均与CK组植株差异不显著;而CF组植株叶片可溶性糖含量随着水淹时间的延长逐渐降低.研究证明,中华蚊母树幼苗生长及生理特性对秋、冬季水淹环境具有一定的调节能力,是中华蚊母树幼苗能够在库区消落带存活生长的一个重要原因.

葱属植物挥发油研究 Ⅲ 薤（Allium chinense G．Don）挥发油成分的研究

利用ＧＣ－ＭＳ联用方法对薤挥发油成分进行了分析，鉴定了１７种化合物，其中１６种为合成化合物。主要成分为二甲基三硫和甲基烯丙基三硫，含量分别为１９．８２％和２３．０６％，后者对血小板聚集有强烈抑制作用。

庐山鹅掌楸(Liriodendron chinense)幼苗蒸腾特性研究

庐山山地上的鹅掌揪幼苗，在夏季晴天土壤供水充足的条件下，其叶片蒸腾速率的日变化为午后高峰型，日蒸腾量为70．92molH2Om-2d-1，最大蒸腾速率达3.9mmolH2Om-21s-1,叶／气温度差、气孔导度、相对湿度等因素对蒸腾速率的影响最显著；鹅掌揪幼苗的水分利用率日平均为4．142mmolCO2mol-1H2o，最高可达11.8mmolCO2mo1-1H2O。