泽兰有效部分L.F04对红细胞流变学的影响

目的研究泽兰有效部分L .F0 4对红细胞流变学的影响 ,以探讨其活血化瘀作用机理。方法用高分子右旋糖酐静脉推注造成大鼠血瘀证动物模型 ,观察泽兰L .F0 4对红细胞变形性、聚集性和膜流动性的影响。结果高分子右旋糖酐造成了大鼠红细胞变形性降低 (P <0 .0 5 ) ,聚集性增高 (P <0 .0 1 ) ,膜流动性降低 (P <0 .0 5 )。与模型组相比 ,L .F0 4大 ( 0 .61 2 g/kg)、小 ( 0 .30 6g/kg)剂量明显改善了红细胞变形性(P <0 .0 5 ) ,抑制了红细胞聚集 (P <0 .0 5 ) ;对红细胞膜流动性有增加的趋势 ,但未呈现出量效关系。结论L .F0 4可显著改善血瘀动物异常的红细胞流变学指标。

推进剂松弛模量主曲线及W.L.F.方程参数的拟合处理

依照最小二乘法原理,提出了由试验数据拟合出推进剂松弛模量主曲线及W.L.F.方程中参数的方法,建立了统一的松弛模量主曲线拟合模型。讨论了QJ2487 93《复合固体推进剂单向拉伸应力松弛模量及主曲线测定方法》标准中所提出的方法,对有关方法进行了对比。文中提出的方法有待于在进一步应用中检验。

Effects of L.F04,the Active Fraction of Lycopus lucidus, on Erythrocytes Rheological Property

Objective: To study the effects of L.F04, the active fraction of Lycopus lucidus, on erythrocytes rheological property so as to investigate its mechanism in promoting blood circulation and removing blood stasis. Method: The effects of L.F04 (used for treatment for 10 days in different dosages) on deformability, aggregation and membrane liquidity of erythrocytes (MLE) as well as whole blood apparent viscosity (η_b) were examined on the basis of rat model of blood-stasis syndrome induced by venous injection of high molecular weight dextran. Result: As compared with the normal control group, the model group's RBC deformability and MLE were lower, and the aggregation of erythrocytes and η_b were higher. Compared with the model group, both L.F04 0.612g/kg and 0.306g/kg showed significant effect in improving deformability and inhibiting aggregation of red blood cells (RBC) and reducing blood viscosity. The trend of improving MLE was also shown. Conclusion: L.F04 could significantly improve the abnormal rheological property of erythrocytes.

Two new steroidal glucosides from Ophiopogon japonicus (L.f.) Ker-Gawl

Two new steroidal glucosides, 26-O-β-D-glucopyranosyl （25S）-furost-5-ene-1β,3β,22α,26-tetraol l-O-β-D-xylopyranosyl- （1 → 3）-[α-h-rhamnopyranosyl-（1 → 2）]-[β-D-fueopyranoside and （25R） spirost-5-ene-3β,14α-diol-3-β-O-β-L-rhanmopyranosyl- （1 → 2）-[β-D-xylopyranosyl（1 → 4）]-[-β-D-glucopyranoside, were isolated from the Ophiopogon japonicus （L.f.） Ker-Gaw. Their structures were elucidated by spectroscopic methods.

Response of a Submersed Macrophyte Hydrilla verticillata (L.f) Royle,to Sodium Dodecylbenzene Sulfonate Stress

The acute toxicity of Sodium dodecylbenzene sulfonate （SDBS） to subcamersed macrophyte Hydrilla verticillata （L.f） Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes： superoxide dismutase （SOD, EC 1.15.1.1）, catalase （CAT, EC 1.11.1.6）, peroxidase （POD, EC 1.11.1.7） in the leaves were investigated under different concentrations of SDBS, The chlorophyll contents in leaves of experimental plants decreased gradually, while the electrical conductivity of culture solution increased gradually with the increased dose of SDBS. Under higher concentrations of SDBS （32 mg/L and 128 mg/L） treatment, SOD lost its activities completely after 24 h. The native PAGE analysis indicated the diversity of POD isoenzymes was obvious under different concentrations of SDBS stress.

生物化学家莱洛伊尔L.F.

著名生化学家莱洛伊尔L.F.(Leloir Luis F.)生于1906年,死于1987年12月4日。他的初期贡献之一且立即引人注目的,是发现了葡萄糖-1,6-二磷酸及葡萄糖-1,6-二磷酸作为磷酸葡萄糖变位酶辅助因子的作用。效仿于此,科里、萨瑟兰和帕斯特纳克一起,提出作为磷酸甘油酸变位酶的2,3双二磷酸甘油酸的类似作用的提议。

The hypoglycemic effect of Lagerstroemia indica L.and Lagerstroemia indica L.f.alba(Nichols.)Rehd.in vitro and in vivo

Lagerstroemia indica L.has a high medicinal value,its bark,leaves and flowers can be used as medicine.Its flowers are reddish,purple,or white,thereinto,the white one is called L.indica Linn.f.alba(Nichols.)Rehd.,which is a forma of L.indica.In this paper,the hypoglycemic effects of different extracts from flowers of L.indica(LIF)and L.indica L.f.alba(Nichols.)Rehd.(LIAF)were investigated byα-glucosidase inhibitory method in vitro and alloxan-induced diabetic mice model in vivo,respectively.The results in vitro showed that ethyl acetate extracts(EA)of LIF and LIAF(IC_(50)=4.45 and 4.09µg/mL,resepectively)had the highest inhibitory activity ofα-glucosidase,and followed by n-butyl alcohol extracts(BU)(IC_(50)=17.01 and 14.58µg/mL,respectively),and the last was petroleum ether extracts(PE)(IC_(50)=103.29 and 112.47µg/mL,respectively),the activities of all extracts were higher than that of the positive control(acarbose,IC_(50)=1278.83µg/mL).The results in vivo showed that compared with the model control group,each dose group of LIF could significantly(P<0.05,P<0.01 or P<0.001)decrease fasting blood glucose,total cholesterol(TC)level and malondialdehyde(MDA)content in diabetic mice and increase superoxide dismutase(SOD)level(P<0.05)in serum.Partial dose groups of LIF could significantly(P<0.05)decrease postprandial blood glucose,triglyceride(TG)and increase liver glycogen content.Each dose group of LIAF could significantly(P<0.05,P<0.01 or P<0.001)decrease fasting blood glucose,TC level and increase SOD level in serum.Partial dose groups of LIAF could significantly(P<0.05)decrease TG,MDA level and increase liver glycogen content.These results indicate that LIF and LIAF had effective prevention and treatment effects on the development for diabetes.

印度Anisochilus carnosus(L.F.)Wall.的体外自由基清除活性(英文)

AIM:To evaluate in vitro anti-oxidant potential of methanolic and aqueous extract of Indian habitants Anisochilus carnosus (L.F.) wall.,which is an annual shrub belonging to the family Lamiaceae commonly known asmint family.METHODS:The present study describes the effects by DPPH,alkaline DMSO,scavenging of ferric ion,NO,H 2 O 2,ABTS models.Both extracts showed good free radical scavenging property which was calculated as IC 50.RESULTS:IC 50 of aqueous and methanolic extracts were found to be 7.09 and 5.77 μg·mL-1 for DPPH,8.14 and 13.29 μg·mL-1 for alkaline DMSO,27.16 and 31.25 μg·mL-1 for scavenging of ferric ion,13.82 and 26.94 μg·mL-1 for NO,13.82 and 26.94 μg·mL-1 for H 2 O 2,113.84 and 128.03 μg·mL-1 for ABTS,which were compared with Ascorbic acid (DPPH,scavenging of ferric ion,NO,ABTS) and rutin (alkaline DMSO,H 2 O 2).Total antioxidant capacity of the extract was found to be 213 and 172 μg·mL-1 ascorbic acid for methanolic and aqueous extracts respectively.CONCLUSION:A.carnosus leaf extracts exhibited potent free radical scavenging activity.The overall antioxidant activity is attributed to its polyphenolic and other phytochemical constituents.The findings suggest that A.carnosus leaves could be a potential source of natural antioxidant in preventing or slowing the progression of aging and age-associated oxidative stress-related degenerative diseases.

Structure analysis of ethyl ferulate from Rubus corchorifolius L.f.leaves and its inhibitory effects on HepG2 liver cancer cells

The tender leaves of Rubus corchorifolius L.f.(R.corchorifolius)have been made into herbal tea for relieving coughs in East and Southeast Asia from ancient times.Herein,we obtained an ethyl acetate extract from R.corchorifolius leaves and isolated a major bioactive compound within.Structural analysis of the isolated compound was performed by spectroscopic methods and the compound was identified as ethyl ferulate.Effect of ethyl ferulate on the growth and proliferation of human liver cancer HepG2 cells and normal human liver LO2 cells was determined by MTT assay.We found that ethyl ferulate significantly suppressed the cell proliferation of HepG2 cells(IC_(50)26.4μM),while it did not cause cytotoxicity on LO2 cells.In addition,flow cytometry analysis demonstrated that ethyl ferulate inhibited HepG2 cell growth via inducing cellular apoptosis.Specifically,ethyl ferulate reduced the electrical potential of the mitochondrial membrane and increased the concentration of calcium ions inside cells.Moreover,these changes were accompanied by increases in the expression of the pro-apoptotic proteins,such as cleaved caspase-3 and Bax.In contrast,cell morphology and growth of LO2 cells remained unaffected.In conclusion,for the first time,our observations demonstrated that ethyl ferulate possesses great potential as an effective and safe antitumor agent for cancer chemoprevention in humans.

红卫兵·作家·文革·改革——答加拿大L.L.F教授问

L:我正在用英文编写“从红卫兵到作家——中国当代作家访问录”一书。我想,在文革时期,你大概是个红卫兵吧? 邓:从年龄上说我应该是红卫兵,但因为父亲被错判入狱,我十三岁就进工厂当童工,成了“工人阶级”,为此只能参加相当于红卫兵性质的工人造反队。 L:这么说当时无论哪个阶层的人都得参加类似红卫兵那样的组织?

浙麦冬愈伤组织的诱导与再生植株的获得

目的:建立浙麦冬组织培养快繁体系,为其规模化生产提供技术支撑。方法:以‘浙麦冬1号’嫩叶基部为外植体,研究植物生长调节剂对诱导愈伤组织、不定芽分化和不定根形成的影响,并进行了炼苗与移栽。结果:‘浙麦冬1号’愈伤组织诱导最适培养基为MS+KT 2.0 mg/L+NAA 0.5 mg/L+蔗糖30.0 g/L+琼脂2.5 g/L+活性炭2.0 g/L,诱导率为92.22%;愈伤组织分化不定芽最适培养基为MS+KT 4.0 mg/L+NAA 0.5 mg/L+蔗糖30.0 g/L+琼脂2.5 g/L+活性炭2.0 g/L,不定芽分化率达88.89%;最适生根培养基为1/2 MS+NAA 0.5 mg/L+蔗糖30.0 g/L+琼脂2.5 g/L,生根率为96.67%;组培苗经驯化,移栽2周后成活率为89.00%。结论:建立了‘浙麦冬1号’组织培养快繁体系。

黄檀属植物的分布、化学成分及药用价值概况

黄檀属植物含有黄酮类、甾醇类以及三萜类等活性成分;具有抗氧化、抗炎、抗菌、成骨活性、脑保护作用、止泻等药理作用。因此,应充分利用黄檀属植物的药用价值,对它进行进一步的研究、开发和利用。

梧桐的药用价值研究进展

梧桐科梧桐属植物梧桐[Firmiana plantanifolia(L.f.)Marsili]的根、叶、花、子在传统医学的记载中均具有较高的药用价值。现代医学研究也从动物实验、临床应用等方面进行了证实,对其化学成分进行了系统的分析,证明其生物活性物质含量丰富。含梧桐组分的制剂市场已有应用,但品种较少。综述了梧桐根、叶、花、子的医药学研究进展,为进一步深入研究梧桐的药用价值提供参考。

基于分子对接虚拟筛选蒙药珍珠杆治疗Ⅱ-型糖尿病有效化学成分

利用分子对接技术虚拟筛选出珍珠杆对Ⅱ-型糖尿病潜在抑制作用的活性化合物。通过数据库以及文献,分子对接软件,AutoDock以及Py MoL软件,以α-葡萄糖苷酶抑制剂(4I8N)、腺苷酸活化蛋白激酶(7MYJ)和蛋白质酪氨酸磷酸激酶(3WY1)为受体蛋白,分别计算出14个化合物的结合能。实验结果显示19-α-hydroxyl-Acetylursolicacid、丁香脂素二葡萄糖苷、山奈酚及金丝桃苷化合物与3个蛋白靶标均具有显著结合能力,可为进一步开发治疗Ⅱ-型糖尿病新型药物做出的指导意义。

ICP-MS法结合化学计量学分析紫菀不同药用部位中无机元素的差异

目的:测定紫菀不同药用部位无机元素含量。方法:采用电感耦合等离子体质谱法(ICP-MS)对紫菀样品中27个无机元素进行定量分析,单因子方差分析、OPLS-DA判别模型的建立与评估分析紫菀根和根茎中无机元素组成的特征元素。结果:与根部位相比,根茎中无机元素Mg、Al、Fe、Ba的含量存在显著差异,V、Cr、Cu、Zn、Ag、Cd、Tl、Ga、U的含量存在极显著差异。紫菀根和根茎中无机元素组成的特征元素为Cu、Cd、Zn、Tl、V、U、Cr、Ag、Ga,且含量差异较大,根中U、V、Cr、Ga、Ag元素大约是根茎中含量的2.7、2.1、1.6、1.6、1.5倍,而根茎中Cu、Cd、Tl、Zn元素大约是根中含量的2.0、1.6、1.5、1.4倍。此外,部分根茎样品中Cu元素含量超标。结论:紫菀不同药用部位无机元素的组成特征可为紫菀药材的质量控制及安全性评价提供依据。

蝙蝠草的研究进展

蝙蝠草是我国壮族的传统民间药材,近年来,随着国内外研究学者对其深入研究,逐步发现蝙蝠草含有的化学成分主要是多糖类、黄酮类、萜类、醇类、生物碱类,其药理作用表现在抗癌、抗疟疾、免疫等。蝙蝠草具有非常重要的研究价值,文章依次就蝙蝠草的本草考证、显微鉴别、化学成分、药理作用进行综述,全面阐述其近几年来的研究进展,为该药材相关的研究和应用提供参考。

青藏高原种植业碳排放量测度——以青海省青稞种植业为例

为研究青藏高原种植业碳排放量测度问题,以青海省青稞(Hordeum vulgare L.var.nudum Hook.f.)种植业产生的碳排放量、碳排放强度、碳成本为测算目标进行测定。通过对青海省1997—2019年的碳排放总量和农业碳排放量现状进行分析,发现青海省碳排放总量仍居高不下,农业碳排放量也始终处于较高水平。基于1997—2019年青海省农业碳排放数据,使用Matlab软件中GUI工具箱搭建非线性次回归神经网络模型,并使用神经网络时序工具对青海省农业2020—2026年碳排放进行多步预测,结果显示2020—2026年青海省农业碳排放量仍呈高位波动增长的趋势,迫切需要开展“双碳”减排。依据青稞种植业碳排放来源,建立了农用柴油、人工、化肥、农药、农膜、N2O排放6个测算指标。并基于2015—2020年各测算指标的数据建立IPCC清单估算模型,最终测得青海省青稞种植业2015—2020年碳排放总量、碳排放强度和碳成本,结果显示,2015—2020年青海省青稞种植业碳排放总量并未随着青稞种植面积的增大而出现大幅度增长,而青稞种植业碳排放强度和碳成本随着青稞种植面积的增大而有所降低。

基于MaxEnt模型与ArcGIS的狗脊贯众潜在分布研究

目的通过研究狗脊蕨Woodwardia japonica(L.f.)Sm.潜在分布预测适生分布区,为其野生资源保护及人工栽培提供依据。方法通过最大熵(MaxEnt)模型与ArcGIS对中国境内狗脊贯众进行生境适生性评价,进行模拟和预测,分析并确定影响狗脊蕨生长的因素,以确定其潜在分布地理位置。结果建立的MaxEnt模型预测结果可靠,筛选出5月降水量、最干季降水量、11月降水量、海拔和10月降水量5个生态因子为影响狗脊蕨生长的主要环境参数,累计贡献率为65.6%,其中5月降水量是影响狗脊蕨分布最主要的生态因子。ArcGIS10.2软件分析显示,高度适生区主要集中在秦岭以南、长江中上游地段及江南丘陵地段。结论MaxEnt模型预测结果与狗脊的实际分布基本一致,该研究结果有助于为狗脊贯众的资源开发利用提供科学依据。

Study of pathogenic genes in a pedigree with familial dilated cardiomyopathy

BACKGROUND Dilated cardiomyopathy(DCM)is a genetically heterogeneous cardiac disorder characterized by left ventricular dilation and contractile dysfunction.The substantial genetic heterogeneity evident in patients with DCM contributes to variable disease severity and complicates overall prognosis,which can be very poor.AIM To identify pathogenic genes in DCM through pedigree analysis.METHODS Our research team identified a patient with DCM in the clinic.Through invest-igation,we found that the family of this patient has a typical DCM pedigree.High-throughput sequencing technology,next-generation sequencing,was used to sequence the whole exomes of seven samples in the pedigree.RESULTS A novel and potentially pathogenic gene mutation-ANK2p.F3067L-was discovered.The mutation was completely consistent with the clinical information for this DCM pedigree.Sanger sequencing was used to further verify the locus of the mutation in pedigree samples.These results were consistent with those of high-throughput sequencing.CONCLUSIONS ANK2p.F3067L is considered a novel and potentially pathogenic gene mutation in DCM.

植物生长调节剂引起的麦冬性状变异与产量形成关系的综合分析

研究植物生长调节剂引起的麦冬[Ophiopogon japonicus（L.f.）Ker-Gawl.]性状变异中影响产量的主要因子,可为合理使用植物生长调节剂、实现麦冬增产提供理论依据。试验通过相关分析、主成分分析、多元线性回归分析和通径分析等方法,分析了影响麦冬产量变化的主要变异因子。结果显示,植物生长调节剂引起的麦冬农艺性状变异系数可达5.1%-31.7%,块根数量和块根长度与麦冬产量间呈极显著的正相关关系,叶鲜重与麦冬产量呈显著的正相关关系,多元线性回归分析筛选出的4个因子对麦冬产量模型的决定系数达0.726。植物生长调节剂实现增产的原因在麦冬性状变化上主要表现为块根数量增多、叶鲜重增大、块根长度增长、叶长变短。