Some wild species of Oryza possess agronomi-cally useful traits such as disease/insect resis-tance and salt tolerance.However,the use ofwild Oryza species for rice improvement hasbeen hampered by the sexual incompatib...Some wild species of Oryza possess agronomi-cally useful traits such as disease/insect resis-tance and salt tolerance.However,the use ofwild Oryza species for rice improvement hasbeen hampered by the sexual incompatibility orsterility of hybrids obtained by conventionalmethods.We have used protoplast fusion as amethod to incorporate useful trait of wild O-ryza species into cultivated rice and~ obtainedsomatic hybrid plants between O.sativa and展开更多
Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The doubl...Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The double-low male sterile lines JL-4 and JL-18 were bred through successive backcrossing of the double -low variety No.4 and No. 18 in Brassica napus L.to NEA. Restorer lines 6720 and 6722 with significant heterosis in F1 were developed. The results from investigating the restoring-ntaintaining relationship and inheritance of the restorer gene indicated that JL-4 and JL-18 were different from both PolCMS and Shan 2A type, and their restorer gene was controlled by a pair of dominant genie genes.展开更多
A support vector regression based on the mean impact value (MIV) model was constructed to identify the bioactive compounds inhibiting proliferation of He La cells in a combination of turmeric (Curcuma longa L.)and liq...A support vector regression based on the mean impact value (MIV) model was constructed to identify the bioactive compounds inhibiting proliferation of He La cells in a combination of turmeric (Curcuma longa L.)and liquorice (Glycyrrhiza) extracts.The quantitative chemical fingerprint from 50 batches of turmeric and liquorice extracts was established using high performance liquid chromatography hyphenated to an ultraviolet visible detector.Qualitative results were obtained using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.A total of 46 peaks (peaks 1–15 from turmeric and 16–46 from liquorice) were selected as "common peaks" for analysis.The inhibitory effect of the combined extracts on He La cells was measured by MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.It was found that 15 compounds (peaks:8,12,30,24,46,11,14,9,3,1,44,18,7,45 and 43)possessing high absolute MIV exhibited a significant correlation with the cytotoxicity against He La cells; most of these have already been confirmed with potential cytotoxicity in previous research.The important potential application of the present model can be extended to help discover active compounds from complex herbal medicine prior to traditional bioassay-guided separation.It is considered that this could be a useful tool for redeveloping herbal medicine based on the use of these active compounds.展开更多
Solanum nigrum L.,belonging to the genus Solanum of Solanaceae family,is widely distributed in temperate and tropical regions of the world.It possesses the effects of clearing away heat and detoxification.A wide spect...Solanum nigrum L.,belonging to the genus Solanum of Solanaceae family,is widely distributed in temperate and tropical regions of the world.It possesses the effects of clearing away heat and detoxification.A wide spectrum of chemical constituents have been isolated from the Solanum nigrum L.,including lignans,steroids,etc.The structures of the eight compounds were determined by comprehensive spectroscopic analyses.It is noteworthy that compounds 1–4,6 and 8 have been isolated from Solanum nigrum L.for the first time.All compounds were evaluated for their antioxidant activities by ABTS and DPPH assays.Based on the results,Solanum nigrum L.could be used as a new natural antioxidant in biomedical applications.展开更多
[Objectives]To make pharmacognostic identification of Euphorbia maculata L.and its related species.[Methods]The classical pharmacognostic identification method was adopted.[Results]The four main medicinal materials ar...[Objectives]To make pharmacognostic identification of Euphorbia maculata L.and its related species.[Methods]The classical pharmacognostic identification method was adopted.[Results]The four main medicinal materials are very similar,the fluff can be seen in E.maculata and Euphorbia thymifolia L.,but not in Euphorbia prostrata Ait.and Euphorbia taihsiensis(Chaw et Koutnik)Oudejians;the tissue structure is basically the same;except for E.taihsiensis without non-glandular hairs,the powder has secretory cells,laticifers,cells,calcium oxalate crystals,fibers,vessels,and seed coat fragments.Through ultrasonic extraction with 80%ethanol,extraction with isobutanol,extending with chloroform∶ethyl acetate∶formic acid(5∶3∶0.3),developing color with 3%aluminum trichloride ethanol solution,under ultraviolet light(365 nm),the fluorescent spots of the same color appeared on the corresponding position of the chromatogram of the reference substance(quercetin,kaempferol).[Conclusions]It is not easy to distinguish the four medicinal materials by character identification and microscopic identification,while the thin-layer chromatography(TLC)is more reliable.展开更多
Botanical morphological characteristics of Physalis alkekengi L.is first introduced,and its medicinal and edible value is elaborated.Cultivation techniques of P.alkekengi L.are mainly introduced.The research aims to p...Botanical morphological characteristics of Physalis alkekengi L.is first introduced,and its medicinal and edible value is elaborated.Cultivation techniques of P.alkekengi L.are mainly introduced.The research aims to provide a certain basis for the better development and application of P.alkekengi L.展开更多
On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical co...On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical composition,pharmacological action and toxicological action,so as to provide a reference basis for further research and further development and utilization of Mongolian medicine.展开更多
Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids ...Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids and flavonoids.Modern pharmacological studies have found that the isolated compounds from S.alopecuroides have a variety of pharmacological activities.In particular,alkaloids represented by matrine,aloperine and oxymatrine have significant anti-inflammatory,anti-viral,anti-tumor,anti-bacterial and immunomodulatory activities.This paper summarized the chemical constituents and pharmacological activities of S.alopecuroides,aiming to provide the basis for further research.展开更多
Parkinson’s disease(PD)is a common neurodegenerative disease.Despite a great deal of research on this disease,there is still no definitive treatment plan.Literature investigations have shown that Piper nigrum L.has a...Parkinson’s disease(PD)is a common neurodegenerative disease.Despite a great deal of research on this disease,there is still no definitive treatment plan.Literature investigations have shown that Piper nigrum L.has a protective effect on the nerve center.Therefore,network pharmacology was used in this study to investigate the therapeutic potential of P.nigrum for PD.As a result,six active alkaloid ingredients and four potential targets were obtained.This study laid the foundation for finding the lead compounds from P.nigrum to treat PD.展开更多
The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. Th...The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.展开更多
Lagerstroemia indica L.has a high medicinal value,its bark,leaves and flowers can be used as medicine.Its flowers are reddish,purple,or white,thereinto,the white one is called L.indica Linn.f.alba(Nichols.)Rehd.,which...Lagerstroemia indica L.has a high medicinal value,its bark,leaves and flowers can be used as medicine.Its flowers are reddish,purple,or white,thereinto,the white one is called L.indica Linn.f.alba(Nichols.)Rehd.,which is a forma of L.indica.In this paper,the hypoglycemic effects of different extracts from flowers of L.indica(LIF)and L.indica L.f.alba(Nichols.)Rehd.(LIAF)were investigated byα-glucosidase inhibitory method in vitro and alloxan-induced diabetic mice model in vivo,respectively.The results in vitro showed that ethyl acetate extracts(EA)of LIF and LIAF(IC_(50)=4.45 and 4.09µg/mL,resepectively)had the highest inhibitory activity ofα-glucosidase,and followed by n-butyl alcohol extracts(BU)(IC_(50)=17.01 and 14.58µg/mL,respectively),and the last was petroleum ether extracts(PE)(IC_(50)=103.29 and 112.47µg/mL,respectively),the activities of all extracts were higher than that of the positive control(acarbose,IC_(50)=1278.83µg/mL).The results in vivo showed that compared with the model control group,each dose group of LIF could significantly(P<0.05,P<0.01 or P<0.001)decrease fasting blood glucose,total cholesterol(TC)level and malondialdehyde(MDA)content in diabetic mice and increase superoxide dismutase(SOD)level(P<0.05)in serum.Partial dose groups of LIF could significantly(P<0.05)decrease postprandial blood glucose,triglyceride(TG)and increase liver glycogen content.Each dose group of LIAF could significantly(P<0.05,P<0.01 or P<0.001)decrease fasting blood glucose,TC level and increase SOD level in serum.Partial dose groups of LIAF could significantly(P<0.05)decrease TG,MDA level and increase liver glycogen content.These results indicate that LIF and LIAF had effective prevention and treatment effects on the development for diabetes.展开更多
文摘Some wild species of Oryza possess agronomi-cally useful traits such as disease/insect resis-tance and salt tolerance.However,the use ofwild Oryza species for rice improvement hasbeen hampered by the sexual incompatibility orsterility of hybrids obtained by conventionalmethods.We have used protoplast fusion as amethod to incorporate useful trait of wild O-ryza species into cultivated rice and~ obtainedsomatic hybrid plants between O.sativa and
文摘Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The double-low male sterile lines JL-4 and JL-18 were bred through successive backcrossing of the double -low variety No.4 and No. 18 in Brassica napus L.to NEA. Restorer lines 6720 and 6722 with significant heterosis in F1 were developed. The results from investigating the restoring-ntaintaining relationship and inheritance of the restorer gene indicated that JL-4 and JL-18 were different from both PolCMS and Shan 2A type, and their restorer gene was controlled by a pair of dominant genie genes.
基金financial support provided by the National Natural Science Foundation of China(No.81102900)
文摘A support vector regression based on the mean impact value (MIV) model was constructed to identify the bioactive compounds inhibiting proliferation of He La cells in a combination of turmeric (Curcuma longa L.)and liquorice (Glycyrrhiza) extracts.The quantitative chemical fingerprint from 50 batches of turmeric and liquorice extracts was established using high performance liquid chromatography hyphenated to an ultraviolet visible detector.Qualitative results were obtained using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.A total of 46 peaks (peaks 1–15 from turmeric and 16–46 from liquorice) were selected as "common peaks" for analysis.The inhibitory effect of the combined extracts on He La cells was measured by MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.It was found that 15 compounds (peaks:8,12,30,24,46,11,14,9,3,1,44,18,7,45 and 43)possessing high absolute MIV exhibited a significant correlation with the cytotoxicity against He La cells; most of these have already been confirmed with potential cytotoxicity in previous research.The important potential application of the present model can be extended to help discover active compounds from complex herbal medicine prior to traditional bioassay-guided separation.It is considered that this could be a useful tool for redeveloping herbal medicine based on the use of these active compounds.
文摘Solanum nigrum L.,belonging to the genus Solanum of Solanaceae family,is widely distributed in temperate and tropical regions of the world.It possesses the effects of clearing away heat and detoxification.A wide spectrum of chemical constituents have been isolated from the Solanum nigrum L.,including lignans,steroids,etc.The structures of the eight compounds were determined by comprehensive spectroscopic analyses.It is noteworthy that compounds 1–4,6 and 8 have been isolated from Solanum nigrum L.for the first time.All compounds were evaluated for their antioxidant activities by ABTS and DPPH assays.Based on the results,Solanum nigrum L.could be used as a new natural antioxidant in biomedical applications.
基金Supported by Program of Key Laboratory of Zhuang and Yao Medicine[Gui Ke Ji Zi[2014]No.32]Project of Guangxi University of Chinese Medicine(H2014015).
文摘[Objectives]To make pharmacognostic identification of Euphorbia maculata L.and its related species.[Methods]The classical pharmacognostic identification method was adopted.[Results]The four main medicinal materials are very similar,the fluff can be seen in E.maculata and Euphorbia thymifolia L.,but not in Euphorbia prostrata Ait.and Euphorbia taihsiensis(Chaw et Koutnik)Oudejians;the tissue structure is basically the same;except for E.taihsiensis without non-glandular hairs,the powder has secretory cells,laticifers,cells,calcium oxalate crystals,fibers,vessels,and seed coat fragments.Through ultrasonic extraction with 80%ethanol,extraction with isobutanol,extending with chloroform∶ethyl acetate∶formic acid(5∶3∶0.3),developing color with 3%aluminum trichloride ethanol solution,under ultraviolet light(365 nm),the fluorescent spots of the same color appeared on the corresponding position of the chromatogram of the reference substance(quercetin,kaempferol).[Conclusions]It is not easy to distinguish the four medicinal materials by character identification and microscopic identification,while the thin-layer chromatography(TLC)is more reliable.
文摘Botanical morphological characteristics of Physalis alkekengi L.is first introduced,and its medicinal and edible value is elaborated.Cultivation techniques of P.alkekengi L.are mainly introduced.The research aims to provide a certain basis for the better development and application of P.alkekengi L.
基金National Natural Science Foundation of China(81560702)Joint Research Project of Inner Mongolia Minzu University and Mongolian Academy of Sciences(NMDGJ0014)。
文摘On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical composition,pharmacological action and toxicological action,so as to provide a reference basis for further research and further development and utilization of Mongolian medicine.
基金the College Student Innovation and Entrepreneurship Training Program(X202010163166).
文摘Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids and flavonoids.Modern pharmacological studies have found that the isolated compounds from S.alopecuroides have a variety of pharmacological activities.In particular,alkaloids represented by matrine,aloperine and oxymatrine have significant anti-inflammatory,anti-viral,anti-tumor,anti-bacterial and immunomodulatory activities.This paper summarized the chemical constituents and pharmacological activities of S.alopecuroides,aiming to provide the basis for further research.
文摘Parkinson’s disease(PD)is a common neurodegenerative disease.Despite a great deal of research on this disease,there is still no definitive treatment plan.Literature investigations have shown that Piper nigrum L.has a protective effect on the nerve center.Therefore,network pharmacology was used in this study to investigate the therapeutic potential of P.nigrum for PD.As a result,six active alkaloid ingredients and four potential targets were obtained.This study laid the foundation for finding the lead compounds from P.nigrum to treat PD.
基金the support of the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq) the Fundacao de Amparo a Pesquisa do Estado de Minas Gerais(FAPEMIG)for financial support the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)for a PVNS.
文摘The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.
基金This work was funded by Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Lagerstroemia indica L.has a high medicinal value,its bark,leaves and flowers can be used as medicine.Its flowers are reddish,purple,or white,thereinto,the white one is called L.indica Linn.f.alba(Nichols.)Rehd.,which is a forma of L.indica.In this paper,the hypoglycemic effects of different extracts from flowers of L.indica(LIF)and L.indica L.f.alba(Nichols.)Rehd.(LIAF)were investigated byα-glucosidase inhibitory method in vitro and alloxan-induced diabetic mice model in vivo,respectively.The results in vitro showed that ethyl acetate extracts(EA)of LIF and LIAF(IC_(50)=4.45 and 4.09µg/mL,resepectively)had the highest inhibitory activity ofα-glucosidase,and followed by n-butyl alcohol extracts(BU)(IC_(50)=17.01 and 14.58µg/mL,respectively),and the last was petroleum ether extracts(PE)(IC_(50)=103.29 and 112.47µg/mL,respectively),the activities of all extracts were higher than that of the positive control(acarbose,IC_(50)=1278.83µg/mL).The results in vivo showed that compared with the model control group,each dose group of LIF could significantly(P<0.05,P<0.01 or P<0.001)decrease fasting blood glucose,total cholesterol(TC)level and malondialdehyde(MDA)content in diabetic mice and increase superoxide dismutase(SOD)level(P<0.05)in serum.Partial dose groups of LIF could significantly(P<0.05)decrease postprandial blood glucose,triglyceride(TG)and increase liver glycogen content.Each dose group of LIAF could significantly(P<0.05,P<0.01 or P<0.001)decrease fasting blood glucose,TC level and increase SOD level in serum.Partial dose groups of LIAF could significantly(P<0.05)decrease TG,MDA level and increase liver glycogen content.These results indicate that LIF and LIAF had effective prevention and treatment effects on the development for diabetes.