Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extract...Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extracts as an antibulbing substance,the amount of which was synchronized with the bulb formation.Since allene oxide synthase inhibitor canceled the antibulbing activity ofα-linolenic acid,it was disclosed that jasmonic acid concerns this regulation.Structure-activity-relationship study revealed that its(3R,7S)stereochemistry is necessary for showing its antibulbing activity.It is concluded that(3R,7S)-jasmonate derived fromα-linolenic acid actually participates in the regulation of bulb formation.展开更多
[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone ...[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.展开更多
选用青海冷地早熟禾(Poa crymophila Keng cv.‘Qinghai’)和青海中华羊茅(Festuca sinensis Keng cv.‘Qinghai’)裸种子、未加保水剂丸粒种子和加有保水剂丸粒种子进行试验,研究干旱胁迫对2种牧草裸种子、未加保水剂丸粒种子和加有保...选用青海冷地早熟禾(Poa crymophila Keng cv.‘Qinghai’)和青海中华羊茅(Festuca sinensis Keng cv.‘Qinghai’)裸种子、未加保水剂丸粒种子和加有保水剂丸粒种子进行试验,研究干旱胁迫对2种牧草裸种子、未加保水剂丸粒种子和加有保水剂丸粒种子幼苗相对生长率、幼苗相对含水量、幼苗中游离脯氨酸质量分数的影响。结果表明,在干旱胁迫下,土壤质量含水量明显降低,导致种子幼苗的相对生长率、相对含水量、以及游离脯氨酸质量分数都受到很大影响。随干旱胁迫的加重,幼苗的相对生长率减小,相对含水量降低,游离脯氨酸质量分数增加。根据隶属函数值分析得出,3类试验的青海冷地早熟禾和青海中华羊茅种子幼苗抗旱性顺序均为加有保水剂丸粒种子>裸种子>未加保水剂丸粒种子。展开更多
基金Supported by the Naito FoundationGrant-in-Aid for Scientific Research (22K05448)from Japan Society for the Promotion of Science.
文摘Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extracts as an antibulbing substance,the amount of which was synchronized with the bulb formation.Since allene oxide synthase inhibitor canceled the antibulbing activity ofα-linolenic acid,it was disclosed that jasmonic acid concerns this regulation.Structure-activity-relationship study revealed that its(3R,7S)stereochemistry is necessary for showing its antibulbing activity.It is concluded that(3R,7S)-jasmonate derived fromα-linolenic acid actually participates in the regulation of bulb formation.
文摘目的测定青藏高原4个主产地蕨麻的营养成分,并对其品质进行综合评价。方法采用SPSS 19.0分别对青藏高原4个主产地蕨麻的营养成分进行方差多重比较分析、主成分分析(principal component analysis,PCA)和聚类分析(hierarchical cluster analysis,HCA),并对蕨麻品质进行综合评价。结果4个不同主产地蕨麻中部分产地蕨麻的各营养成分之间差异显著(P<0.05),普遍具有高膳食纤维(平均含量>6 g/100 g)、低脂肪和低饱和脂肪(平均含量分别<3 g/100 g和<1.5 g/100 g)、极低钠(平均含量<40 mg/100 g)、氨基酸组成接近联合国粮食及农业组织和世界卫生组织(Food and Agriculture Organization of the United Nations/World Health Organization,FAO/WHO)的理想模式、脂肪酸种类丰富(含10种主要脂肪酸)、富含多种矿物质元素[平均Fe、Mg、K含量≥30%营养素参考值(nutrient reference values,NRV)]等特点。其中青海蕨麻为高蛋白食品(平均含量>20%NRV),四川和西藏蕨麻含有多种维生素(平均维生素B_(1)、维生素B_(2)、叶酸含量≥15%NRV)。PCA综合评价结果表明青海蕨麻排名第一,HCA将4个不同主产地蕨麻分为3类,第Ⅰ类蕨麻综合品质相对更好。结论蕨麻具有良好的营养价值和开发前景,青海蕨麻营养品质最佳,可为其质量控制提供依据。
文摘[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.
文摘选用青海冷地早熟禾(Poa crymophila Keng cv.‘Qinghai’)和青海中华羊茅(Festuca sinensis Keng cv.‘Qinghai’)裸种子、未加保水剂丸粒种子和加有保水剂丸粒种子进行试验,研究干旱胁迫对2种牧草裸种子、未加保水剂丸粒种子和加有保水剂丸粒种子幼苗相对生长率、幼苗相对含水量、幼苗中游离脯氨酸质量分数的影响。结果表明,在干旱胁迫下,土壤质量含水量明显降低,导致种子幼苗的相对生长率、相对含水量、以及游离脯氨酸质量分数都受到很大影响。随干旱胁迫的加重,幼苗的相对生长率减小,相对含水量降低,游离脯氨酸质量分数增加。根据隶属函数值分析得出,3类试验的青海冷地早熟禾和青海中华羊茅种子幼苗抗旱性顺序均为加有保水剂丸粒种子>裸种子>未加保水剂丸粒种子。
