The effects of different Cd (Cadmium) levels on generation of active oxygen speceies(AOS) and H2O2-scavenging system in the leaves of Brassica campestris L. ssp. chinensiswere studied. The results showed that generat...The effects of different Cd (Cadmium) levels on generation of active oxygen speceies(AOS) and H2O2-scavenging system in the leaves of Brassica campestris L. ssp. chinensiswere studied. The results showed that generation rate, and H2O2 content were enhancedand malondialdehyde (MDA) content increased with the increase of Cd concentrations inthe growth medium. The activities of ascorbate peroxidase (APX), dehydroascorbatereductase (DR) and glutathione reductase (GR) were promoted by the addition of Cd.Exposed to Cd also increased the contents of ascorbate (AsA) and glutathione (GSH) in theleaves.展开更多
A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers inclu...A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers including 265 AFLP markers and 87 RAPD markers were integrated into 17 linkage groups. It covered a total of 2 665. 7 cM with an average interval of 7. 6 cM. AFLP marker is efficient for map construction while it easily forms clusters to cause big gaps in map. A total of 13.92 % abnormal segregation markers distributed in the map. The molecular genetic map is fundamental for gene localization, comparative genomics, and QTL mapping of important agronomic traits.展开更多
To assess the effects of N fertilizer ammonium sulphate nitrate [(NH4)2SO4 plus NH4NO3;ASN] with the new nitrification inhibitor (NI) 3, 4-dimethylpyrazole phosphate (DMPP)(ASN+DMPP) on yield, nitrate accumulation, an...To assess the effects of N fertilizer ammonium sulphate nitrate [(NH4)2SO4 plus NH4NO3;ASN] with the new nitrification inhibitor (NI) 3, 4-dimethylpyrazole phosphate (DMPP)(ASN+DMPP) on yield, nitrate accumulation, and quality of cabbage (Brassica campastrisL. ssp. pekinesis), two field trials were carried out under various soil-climaticconditions in Jinhua City and Xinchang County, Zhejiang Province of China in 2002.Results showed that DMPP could increase the mean yield by+2.0tha-1 in Jinhua, +5.5tha-1 inXinchang, decrease NO3--N content by -9.4% in Jinhua, -7.3% in Xinchang and improvenutritional quality by increasing vitamin C (VC), soluble sugar, K, Fe, Zn contentssignificantly.展开更多
The study analyzed the silencing of BcMF12 gene regulated by BcA9 promoter in the transgenic pakchoi and confirmed the effect of antisense BcMF12 gene on the pollen development. A conserved BcMF12 gene fragment was am...The study analyzed the silencing of BcMF12 gene regulated by BcA9 promoter in the transgenic pakchoi and confirmed the effect of antisense BcMF12 gene on the pollen development. A conserved BcMF12 gene fragment was amplified from the cDNA of flower buds in pakchoi （Brassica campestris L. ssp. chinensis, syn. B. rapa L. ssp. chinensis） and was fused to the anther specific BcA9 promoter. The plant antisense expression vector was constructed and then introduced into pakchoi via Agrobacterium-mediated transformation. The transgenic plants were screened by antibiotics and molecular analysis. PCR and Southern blot revealed that the antisense BcMF12-GUS fusion gene regulated by BcA9 promoter was integrated into transgenic plants. Northern blot suggested that the expression of BcMF12 gene was down-regulated significantly. The pollen germination rate of transgenic plants with antisense BcMF12 gene decreased as compared with that of the control plants. The expression of the gene BcMF12 related to the pollen development was inhibited by the antisense BcMF12 driven by BcA9 promoter, which consequently affected the pollen development in pakchoi.展开更多
通过田间试验研究了红菜薹(Brassica campestris L. ssp. chinensis var. utili Tsen et Lee)废弃物还田以及化肥减施对四季豆产量和养分累积的影响。结果表明,在常规施肥的基础上,红菜薹废弃物半量和全量还田四季豆产量分别增加了13.41...通过田间试验研究了红菜薹(Brassica campestris L. ssp. chinensis var. utili Tsen et Lee)废弃物还田以及化肥减施对四季豆产量和养分累积的影响。结果表明,在常规施肥的基础上,红菜薹废弃物半量和全量还田四季豆产量分别增加了13.41%和29.43%,与红菜薹废弃物全部还田相比,全部还田+化肥用量减施30%使得四季豆产量降低了25.41%;与单施化肥相比,全部还田+化肥用量减施30%处理四季豆生物量增加了9.38%。红菜薹还田氮素、磷素和钾素累积量分别较单施化肥处理增加了18.72%~32.55%、6.35%~38.22%和5.73%~41.87%,且富集的氮、钾主要存在茎、叶片和果实中。展开更多
文摘The effects of different Cd (Cadmium) levels on generation of active oxygen speceies(AOS) and H2O2-scavenging system in the leaves of Brassica campestris L. ssp. chinensiswere studied. The results showed that generation rate, and H2O2 content were enhancedand malondialdehyde (MDA) content increased with the increase of Cd concentrations inthe growth medium. The activities of ascorbate peroxidase (APX), dehydroascorbatereductase (DR) and glutathione reductase (GR) were promoted by the addition of Cd.Exposed to Cd also increased the contents of ascorbate (AsA) and glutathione (GSH) in theleaves.
文摘A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers including 265 AFLP markers and 87 RAPD markers were integrated into 17 linkage groups. It covered a total of 2 665. 7 cM with an average interval of 7. 6 cM. AFLP marker is efficient for map construction while it easily forms clusters to cause big gaps in map. A total of 13.92 % abnormal segregation markers distributed in the map. The molecular genetic map is fundamental for gene localization, comparative genomics, and QTL mapping of important agronomic traits.
基金supported by the National Natural Science Foundation of China(30370838)Science and Technology Committee of Zhejiang Province,China(021102084)BASF Company of Germany.
文摘To assess the effects of N fertilizer ammonium sulphate nitrate [(NH4)2SO4 plus NH4NO3;ASN] with the new nitrification inhibitor (NI) 3, 4-dimethylpyrazole phosphate (DMPP)(ASN+DMPP) on yield, nitrate accumulation, and quality of cabbage (Brassica campastrisL. ssp. pekinesis), two field trials were carried out under various soil-climaticconditions in Jinhua City and Xinchang County, Zhejiang Province of China in 2002.Results showed that DMPP could increase the mean yield by+2.0tha-1 in Jinhua, +5.5tha-1 inXinchang, decrease NO3--N content by -9.4% in Jinhua, -7.3% in Xinchang and improvenutritional quality by increasing vitamin C (VC), soluble sugar, K, Fe, Zn contentssignificantly.
基金the National Natural Science Foundation of China(30671426)the Key Sci-Technology Project of Zhejiang Province,China(2005C12019-02)
文摘The study analyzed the silencing of BcMF12 gene regulated by BcA9 promoter in the transgenic pakchoi and confirmed the effect of antisense BcMF12 gene on the pollen development. A conserved BcMF12 gene fragment was amplified from the cDNA of flower buds in pakchoi （Brassica campestris L. ssp. chinensis, syn. B. rapa L. ssp. chinensis） and was fused to the anther specific BcA9 promoter. The plant antisense expression vector was constructed and then introduced into pakchoi via Agrobacterium-mediated transformation. The transgenic plants were screened by antibiotics and molecular analysis. PCR and Southern blot revealed that the antisense BcMF12-GUS fusion gene regulated by BcA9 promoter was integrated into transgenic plants. Northern blot suggested that the expression of BcMF12 gene was down-regulated significantly. The pollen germination rate of transgenic plants with antisense BcMF12 gene decreased as compared with that of the control plants. The expression of the gene BcMF12 related to the pollen development was inhibited by the antisense BcMF12 driven by BcA9 promoter, which consequently affected the pollen development in pakchoi.
文摘通过田间试验研究了红菜薹(Brassica campestris L. ssp. chinensis var. utili Tsen et Lee)废弃物还田以及化肥减施对四季豆产量和养分累积的影响。结果表明,在常规施肥的基础上,红菜薹废弃物半量和全量还田四季豆产量分别增加了13.41%和29.43%,与红菜薹废弃物全部还田相比,全部还田+化肥用量减施30%使得四季豆产量降低了25.41%;与单施化肥相比,全部还田+化肥用量减施30%处理四季豆生物量增加了9.38%。红菜薹还田氮素、磷素和钾素累积量分别较单施化肥处理增加了18.72%~32.55%、6.35%~38.22%和5.73%~41.87%,且富集的氮、钾主要存在茎、叶片和果实中。
