Allium cepa L.var.agrogatum Don(ALAP)is commonly consumed in China as well as other regions and has various beneficial health effects.A novel acidic polysaccharide(named ALAP-21)was obtained from ALAP by ultrasonic an...Allium cepa L.var.agrogatum Don(ALAP)is commonly consumed in China as well as other regions and has various beneficial health effects.A novel acidic polysaccharide(named ALAP-21)was obtained from ALAP by ultrasonic and microwave-assisted extraction and purification using DEAE-52 anion exchange and Sephadex G-100 columns.The monosaccharide composition,structural and antioxidative properties of ALAP-21 were investigated by GC–MS chromatography,FT-IR and NMR spectroscopies and three antioxidative activity tests in vitro.The results showed that ALAP-21 was a heteropolysaccharide composed of glucose,galacturonic acid,mannose,galactose,arabinose,rhamnose,xylose,fructose and glucuronic acid with a relative molar ratio of 26.282:27.546:11.400:4.781:2.467:2.445:3.622:1.106:1.753,owning(1→4)-β-d-Glcp,(1→4)-β-d-GalAp6Me3OA,(1→4)-β-d-Galp6OMe,(1→2)-β-l-Rhap,(1→4)-β-d-Manp glycosidic linkages.(1→4)-β-d-Glcp and(1→4)-β-d-GalAp6Me3OA residues might be the main components of the sugar chain backbone of ALAP-21.Furthermore,ALAP-21 exhibited high potential for DPPH radicals(82.02%),hydroxyl radicals(53.33%)and superoxide anion radicals(50.28%).These results provide a reference for further research and rational development of ALAP polysaccharides.展开更多
The biomass and net primary production of Mongolian scotch pine (Pinus sylvestris L. var mongolica) plantations of west Kerqin sandy land were measured. According to average standard trees, the biomass, netprimary pro...The biomass and net primary production of Mongolian scotch pine (Pinus sylvestris L. var mongolica) plantations of west Kerqin sandy land were measured. According to average standard trees, the biomass, netprimary production and their distributions of trunk, bark, branch, leaf and root of 16-year-old stand were analyzed.The regressive equation for the estimation of each organ biomass was established through djmensional analysis.Preferable equation with higher precision was selected. The study results showed that the total biomass of theforest community was 62.023 t/hm2 and net primary production was 5.045 V(hm2. a). which indicates that thecommunity of plantation possesses high bio-productivity.展开更多
The effects of treatment of chlorine dioxide (C1Oz) gas on postharvest physiology and preservation quality of green bell peppers were studied. Green bell peppers were collected in bags and treated with 0, 5, 10, 20,...The effects of treatment of chlorine dioxide (C1Oz) gas on postharvest physiology and preservation quality of green bell peppers were studied. Green bell peppers were collected in bags and treated with 0, 5, 10, 20, and 50 mg L^-1 ClO2 gas at 10±0.5℃ for over 40 d, and the changes in postharvest physiology and preservation quality of the peppers were evaluated during the storage. The inhibition of rot of the peppers was observed for all the tested ClO2 gas treatments. The rot rates of the treated samples were 50% lesser than those of the control after day 40 of storage. The highest inhibitory effect was obtained after 50 mg L^-1 ClO2 gas treatment, where the peppers did not decay until day 30 and showed only one-fourth of the rot rate of the control at day 40 of storage. The respiratory activity of the peppers was significantly (P〈0.05) inhibited by 20 and 50 mg L^-1 ClO2 treatments, whereas no significant effects on respiratory activity were observed with 5 and 10 mg L^-1 ClO2 treatments (P〉0.05). Except for 50 mg L^-1 ClO2, malondialdenyde (MDA) contents in the peppers treated with 5, 10, or 20 mg L^-1 ClO2 were not significantly (P〉0.05) different from those in the control. Degradation of chlorophyll in the peppers was delayed by 5 mg L-1ClO2, but promoted by 10, 20, or 50 mg L^-1 ClO2. The vitamin C content, titratable acidity, and total soluble solids of the peppers treated by all the tested ClO2 gas did not significantly change during the storage. The results suggested that ClO2 gas treatment effectively delayed the postharvest physiological transformation of green peppers, inhibited decay and respiration, maintained some nutritional and sensory quality, and retarded MDA accumulation.展开更多
AIM: To investigate the protective effects and possible mechanisms of Veratrum nigrum L.var. ussuriense Nakai alkaloids (VnA) on hepatic ischemia/reperfusion (I/R) injury in rats. METHODS: Forty male Wistar rats were ...AIM: To investigate the protective effects and possible mechanisms of Veratrum nigrum L.var. ussuriense Nakai alkaloids (VnA) on hepatic ischemia/reperfusion (I/R) injury in rats. METHODS: Forty male Wistar rats were randomly divided into four experimental groups (n = 10 in each): (A) Control group (the sham operation group); (B) I/R group (pretreated with normal saline); (C) Small-dose (10 mg/kg) VnA pretreatment group; (D) Large-dose (20 mg/kg) VnA pretreatment group. Hepatic ischemia/ reperfusion (Hepatic I/R) was induced by occlusion of the portal vein and the hepatic artery for 90 min, followed by reperfusion for 240 min. The pretreatment groups were administered with VnA intraperitoneally, 30 min before surgery, while the control group and I/R group were given equal volumes of normal saline. Superoxide dismutase (SOD) activity, myeloperoxidase (MPO) activity and nitric oxide (NO) content in the liver tissue at the end of reperfusion were determined and liver function was measured. The expression of intercellular adhesion molecule-1 (ICAM-1) and E-selectin (ES) were detected by immunohistochemical examinations and Western blot analyses. RESULTS: The results showed that hepatic I/R elicited a significant increase in the plasma levels of alanine aminotransferase (ALT: 74.53 ± 2.58 IU/L vs 1512.54 ± 200.76 IU/L, P < 0.01) and lactic dehydrogenase (LDH: 473.48 ± 52.17 IU/L vs 5821.53 ± 163.69 IU/L, P < 0.01), as well as the levels of MPO (1.97 ± 0.11U/g vs 2.57 ± 0.13 U/g, P < 0.01) and NO (69.37 ± 1.52 mmol/g protein vs 78.39 ± 2.28 mmol/g protein, P < 0.01) in the liver tissue, all of which were reduced by pretreatment with VnA, respectively (ALT: 1512.54 ± 200.76 IU/L vs 977.93 ± 89.62 IU/L, 909.81 ± 132.76 IU/L, P < 0.01, P < 0.01; LDH: 5821.53 ± 163.69 IU/L vs 3015.44 ± 253.01 IU/L, 2448.75 ± 169.4 IU/L, P < 0.01, P < 0.01; MPO: 2.57 ± 0.13 U/g vs 2.13 ± 0.13 U/g, 2.07 ± 0.05 U/g, P < 0.01, P < 0.01; NO: 78.39 ± 2.28 mmol/g protein vs 71.11 ± 1.73 mmol/g protein, 68.58 ± 1.95 mmol/g protein, P < 0.05, P < 0.01). The activity of SOD (361.75 ± 16.22 U/mg protein vs 263.19 ± 12.10 U/mg protein, P < 0.01) in the liver tissue was decreased after I/R, which was enhanced by VnA pretreatment (263.19 ± 12.10 U/mg protein vs 299.40 ± 10.80 U/mg protein, 302.09 ± 14.80 U/mg protein, P < 0.05, P < 0.05). Simultaneously, the histological evidence of liver hemorrhage, polymorphonuclear neutrophil infiltration and the overexpression of ICAM-1 and E-selectin in the liver tissue were observed, all of which were attenuated in the VnA pretreated groups.CONCLUSION: The results demonstrate that VnA pretreatment exerts significant protection against hepatic I/R injury in rats. The protective effects are possibly associated with enhancement of antioxidant capacity, reduction of inflammatory responses and suppressed expression of ICAM-1 and E-selectin.展开更多
Objective To investigate the anti-oxidant and anti-inflammatory effects of ethanol extract of Polygala sibirica L.var megalopha Fr.(EEP)on RAW264.7 mouse macrophages.Methods RAW264.7 cells were pretreated with 0–200&...Objective To investigate the anti-oxidant and anti-inflammatory effects of ethanol extract of Polygala sibirica L.var megalopha Fr.(EEP)on RAW264.7 mouse macrophages.Methods RAW264.7 cells were pretreated with 0–200µg/mL EEP or vehicle for 2 h prior to exposure to 1µg/mL lipopolysaccharide(LPS)for 24 h.Nitric oxide(NO)and prostaglandin(PGE2)production were determined by Griess reagent and enzyme-linked immunosorbent assay(ELISA),respectively.The mRNA levels of inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),tumor necrosis factorα(TNF-α),interleukin-1beta(IL-1β),and IL-6 were determined using reverse transcription polymerase chain reaction(RT-PCR).Western blot assay was used to determine the protein expressions of iNOS,COX-2,phosphorylation of extracellular regulated protein kinases(ERK1/2),c-Jun N-terminal kinase(JNK),inhibitory subunit of nuclear factor Kappa B alpha(IκB-α)and p38.Immunofluorescence was used to observe the nuclear expression of nuclear factor-κB p65(NF-κB p65).Additionally,the anti-oxidant potential of EEP was evaluated by reactive oxygen species(ROS)production and the activities of catalase(CAT)and superoxide dismutase(SOD).The 2,2-diphenyl-1-picrylhydrazyl(DPPH),hydroxyl(OH),superoxide anion(O2−)radical and nitrite scavenging activity were also measured.Results The total polyphenol and flavonoid contents of EEP were 23.50±2.16 mg gallic acid equivalent/100 g and 43.78±3.81 mg rutin equivalent/100 g.With EEP treatment(100 and 150µg/mL),there was a notable decrease in NO and PGE2 production induced by LPS in RAW264.7 cells by downregulation of iNOS and COX-2 mRNA and protein expressions(P<0.01 or P<0.05).Furthermore,with EEP treatment(150µg/mL),there was a decrease in the mRNA expression levels of TNF-α,IL-1βand IL-6,as well as in the phosphorylation of ERK,JNK and p38 mitogen-activated protein kinase(MAPK,P<0.01 or P<0.05),by blocking the nuclear translocation of NF-κB p65 in LPS-stimulated cells.In addition,EEP(100 and 150µg/mL)led to an increase in the anti-oxidant enzymes activity of SOD and CAT,with a concomitant decrease in ROS production(P<0.01 or P<0.05).EEP also indicated the DPPH,OH,O2−radical and nitrite scavenging activity.Conclusion EEP inhibited inflammatory responses in activated macrophages through blocking MAPK/NF-κB pathway and protected against oxidative stress.展开更多
Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory p...Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory properties and can be used to treat acute lung injury and eczema.The aim of this study is to solve the problems of extraction and purification of active components from PAF. Materials and Methods: The solvent to be used for extraction and its concentration, the solid-to-liquid ratio, and extraction duration were investigated using a single-factor experiment.An orthogonal design(L_(9)[3^(4)]) was used to determine the optimum extraction conditions. After optimization, the sample’s concentrations and flow velocity, the eluents and their velocity, adsorption time, and the removed water volume were measured. The content of the five steroids in the sample was determined by high-performance liquid chromatography(HPLC). We also investigated the anti-inflammatory property of PAF calyxes before and after purification. Results: The optimum extraction and purification processes were determined by single-factor analysis.AB-8 was identified as the best macroporous adsorption resin for enrichment. After optimization, the average total steroid content was 71.83%,and the average recovery was 90% after purification. Among the five steroid components detected by HPLC, physalin F showed the highest content. Furthermore, the sample obtained after purification could significantly inhibit paw edema by egg whites induced. Conclusions: An environmentally-sustainable, efficient, and stable process was first optimized for enriching and purifying total steroids from PAF. The process has the potential for further development and utilization in the pharmaceutical industry.展开更多
为进一步探明陕西省榆林市樟子松人工林的土壤养分变化特征,以5年生樟子松(Pinus sylvestris L. var. mongolica Litv.)纯林和5年生樟子松-胡枝子(Lespedeza bicolor Turcz.)混交林为研究对象,分析土壤有机质、硝态氮、有效磷、速效钾...为进一步探明陕西省榆林市樟子松人工林的土壤养分变化特征,以5年生樟子松(Pinus sylvestris L. var. mongolica Litv.)纯林和5年生樟子松-胡枝子(Lespedeza bicolor Turcz.)混交林为研究对象,分析土壤有机质、硝态氮、有效磷、速效钾等理化指标以及土壤酶活性的变化特征。结果表明,樟子松纯林和樟子松-胡枝子混交林土壤有机质、有效磷和速效钾含量整体上均随着土层深度的增加呈下降趋势,全磷含量均随着土层深度的增加呈先升高后降低的趋势,硝态氮含量则均随着土壤深度的增加而增加,5个指标均表现为樟子松-胡枝子混交林高于樟子松纯林,且樟子松-胡枝子混交林各层土壤有效磷含量均显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤含水率各土层间差异均不显著,樟子松-胡枝子混交林5~15 cm土层土壤含水率含量显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤碱性磷酸酶活性、过氧化氢酶活性、脲酶活性均随着土层深度的增加而降低,且樟子松-胡枝子混交林过氧化氢酶活性和脲酶活性在各土层均显著高于樟子松纯林(P<0.05)。展开更多
Carotenoids and chlorophylls are among the most widely distributed pigments in nature that play essential roles in the photosynthetic apparatus and confer diverse colours in plants.Among all vegetables,cauliflower(Bra...Carotenoids and chlorophylls are among the most widely distributed pigments in nature that play essential roles in the photosynthetic apparatus and confer diverse colours in plants.Among all vegetables,cauliflower(Brassica oleracea L.ssp.var.botrytis)is rich in phytochemicals and is an important crop grown all over the world.This study investigates carotenoid and chlorophyll concentrations in differently pigmented cultivars and elucidates the role of transcriptional regulation of carotenoid accumulation including lutein andβ-carotene.Here,we characterised changes in pigments by UHPLC-DAD-ToF-MS and changes in transcript levels of carotenoid metabolic genes by qRT-PCR in florets and leaves of orange(‘Jaffa'and‘Sunset'),purple(‘Di Sicilia Violetto'and‘Graffiti'),green(‘Trevi')and white(‘Clapton')cultivars.Transcript levels of all carotenoid metabolic genes showed different transcript level patterns in the leaves and florets.Compared to the other cultivars,the orange cultivars had the highest levels ofβ-carotene in the florets and lutein in the leaves resulting in changes lutein/β-carotene ratios.In the green cultivar,higher transcript levels were also found,especially for phytoene synthase and phytoene desaturase genes of the core biosynthesis pathway.However,no increased carotenoid concentrations were observed,possibly due to a higher carotenoid turnover induced by the carotenoid cleavage dioxygenase 4 in the green cultivar.In the white(‘Clapton')and purple(‘Di Sicilia Violetto'and‘Graffiti')cultivars the phytoene desaturase transcript levels as well as carotenoid concentrations were low.Chlorophyll concentrations changed in trend comparable to the carotenoid concentrations and were only significantly lower in the leaves of the orange cultivar‘Jaffa'.Also,the chlorophyll a/b ratio changed in‘Jaffa'.In florets the highest chlorophylls concentrations were observed for the green cultivar(‘Trevi')and the purple cultivar(‘Di Sicilia Violetto').Taken together,the study demonstrates the complex source-sink relationship of carotenoid accumulation in different coloured cauliflower.展开更多
Coix seed is a dry and mature seed of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf in the Gramineae family.Coix seed has a sweet,light taste,and a cool nature.Coix seed enters the spleen,stomach,and lung meridians.It ...Coix seed is a dry and mature seed of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf in the Gramineae family.Coix seed has a sweet,light taste,and a cool nature.Coix seed enters the spleen,stomach,and lung meridians.It has the effects of promoting diuresis and dampness,strengthening the spleen to prevent diarrhea,removing arthralgia,expelling pus,and detoxifying and dispersing nodules.It is used for the treatment of edema,athlete's foot,poor urination,spleen deficiency and diarrhea,dampness and obstruction,lung carbuncle,intestinal carbuncle,verruca,and cancer.The medicinal and health value is high,and it has been included in the list of medicinal and food sources in China,which has a large development and application space.This article reviews the current research achievements in the processing methods and anti-tumor activities of Coix seed and provides examples of its clinical application in ancient and modern times,aiming to provide reference for further research on Coix seed and contribute to its clinical application and development.Through the analysis of the traditional Chinese patent medicines,and simple preparations and related health food of Coix seed queried by Yaozhi.com,the source,function,and dosage form of Coix seed were comprehensively analyzed,with a view of providing a reference for the development of Coix seed medicine and food.展开更多
基金the National Science and Technology Support Program of China(Grant No.2013BAD16B08).
文摘Allium cepa L.var.agrogatum Don(ALAP)is commonly consumed in China as well as other regions and has various beneficial health effects.A novel acidic polysaccharide(named ALAP-21)was obtained from ALAP by ultrasonic and microwave-assisted extraction and purification using DEAE-52 anion exchange and Sephadex G-100 columns.The monosaccharide composition,structural and antioxidative properties of ALAP-21 were investigated by GC–MS chromatography,FT-IR and NMR spectroscopies and three antioxidative activity tests in vitro.The results showed that ALAP-21 was a heteropolysaccharide composed of glucose,galacturonic acid,mannose,galactose,arabinose,rhamnose,xylose,fructose and glucuronic acid with a relative molar ratio of 26.282:27.546:11.400:4.781:2.467:2.445:3.622:1.106:1.753,owning(1→4)-β-d-Glcp,(1→4)-β-d-GalAp6Me3OA,(1→4)-β-d-Galp6OMe,(1→2)-β-l-Rhap,(1→4)-β-d-Manp glycosidic linkages.(1→4)-β-d-Glcp and(1→4)-β-d-GalAp6Me3OA residues might be the main components of the sugar chain backbone of ALAP-21.Furthermore,ALAP-21 exhibited high potential for DPPH radicals(82.02%),hydroxyl radicals(53.33%)and superoxide anion radicals(50.28%).These results provide a reference for further research and rational development of ALAP polysaccharides.
文摘The biomass and net primary production of Mongolian scotch pine (Pinus sylvestris L. var mongolica) plantations of west Kerqin sandy land were measured. According to average standard trees, the biomass, netprimary production and their distributions of trunk, bark, branch, leaf and root of 16-year-old stand were analyzed.The regressive equation for the estimation of each organ biomass was established through djmensional analysis.Preferable equation with higher precision was selected. The study results showed that the total biomass of theforest community was 62.023 t/hm2 and net primary production was 5.045 V(hm2. a). which indicates that thecommunity of plantation possesses high bio-productivity.
文摘The effects of treatment of chlorine dioxide (C1Oz) gas on postharvest physiology and preservation quality of green bell peppers were studied. Green bell peppers were collected in bags and treated with 0, 5, 10, 20, and 50 mg L^-1 ClO2 gas at 10±0.5℃ for over 40 d, and the changes in postharvest physiology and preservation quality of the peppers were evaluated during the storage. The inhibition of rot of the peppers was observed for all the tested ClO2 gas treatments. The rot rates of the treated samples were 50% lesser than those of the control after day 40 of storage. The highest inhibitory effect was obtained after 50 mg L^-1 ClO2 gas treatment, where the peppers did not decay until day 30 and showed only one-fourth of the rot rate of the control at day 40 of storage. The respiratory activity of the peppers was significantly (P〈0.05) inhibited by 20 and 50 mg L^-1 ClO2 treatments, whereas no significant effects on respiratory activity were observed with 5 and 10 mg L^-1 ClO2 treatments (P〉0.05). Except for 50 mg L^-1 ClO2, malondialdenyde (MDA) contents in the peppers treated with 5, 10, or 20 mg L^-1 ClO2 were not significantly (P〉0.05) different from those in the control. Degradation of chlorophyll in the peppers was delayed by 5 mg L-1ClO2, but promoted by 10, 20, or 50 mg L^-1 ClO2. The vitamin C content, titratable acidity, and total soluble solids of the peppers treated by all the tested ClO2 gas did not significantly change during the storage. The results suggested that ClO2 gas treatment effectively delayed the postharvest physiological transformation of green peppers, inhibited decay and respiration, maintained some nutritional and sensory quality, and retarded MDA accumulation.
文摘AIM: To investigate the protective effects and possible mechanisms of Veratrum nigrum L.var. ussuriense Nakai alkaloids (VnA) on hepatic ischemia/reperfusion (I/R) injury in rats. METHODS: Forty male Wistar rats were randomly divided into four experimental groups (n = 10 in each): (A) Control group (the sham operation group); (B) I/R group (pretreated with normal saline); (C) Small-dose (10 mg/kg) VnA pretreatment group; (D) Large-dose (20 mg/kg) VnA pretreatment group. Hepatic ischemia/ reperfusion (Hepatic I/R) was induced by occlusion of the portal vein and the hepatic artery for 90 min, followed by reperfusion for 240 min. The pretreatment groups were administered with VnA intraperitoneally, 30 min before surgery, while the control group and I/R group were given equal volumes of normal saline. Superoxide dismutase (SOD) activity, myeloperoxidase (MPO) activity and nitric oxide (NO) content in the liver tissue at the end of reperfusion were determined and liver function was measured. The expression of intercellular adhesion molecule-1 (ICAM-1) and E-selectin (ES) were detected by immunohistochemical examinations and Western blot analyses. RESULTS: The results showed that hepatic I/R elicited a significant increase in the plasma levels of alanine aminotransferase (ALT: 74.53 ± 2.58 IU/L vs 1512.54 ± 200.76 IU/L, P < 0.01) and lactic dehydrogenase (LDH: 473.48 ± 52.17 IU/L vs 5821.53 ± 163.69 IU/L, P < 0.01), as well as the levels of MPO (1.97 ± 0.11U/g vs 2.57 ± 0.13 U/g, P < 0.01) and NO (69.37 ± 1.52 mmol/g protein vs 78.39 ± 2.28 mmol/g protein, P < 0.01) in the liver tissue, all of which were reduced by pretreatment with VnA, respectively (ALT: 1512.54 ± 200.76 IU/L vs 977.93 ± 89.62 IU/L, 909.81 ± 132.76 IU/L, P < 0.01, P < 0.01; LDH: 5821.53 ± 163.69 IU/L vs 3015.44 ± 253.01 IU/L, 2448.75 ± 169.4 IU/L, P < 0.01, P < 0.01; MPO: 2.57 ± 0.13 U/g vs 2.13 ± 0.13 U/g, 2.07 ± 0.05 U/g, P < 0.01, P < 0.01; NO: 78.39 ± 2.28 mmol/g protein vs 71.11 ± 1.73 mmol/g protein, 68.58 ± 1.95 mmol/g protein, P < 0.05, P < 0.01). The activity of SOD (361.75 ± 16.22 U/mg protein vs 263.19 ± 12.10 U/mg protein, P < 0.01) in the liver tissue was decreased after I/R, which was enhanced by VnA pretreatment (263.19 ± 12.10 U/mg protein vs 299.40 ± 10.80 U/mg protein, 302.09 ± 14.80 U/mg protein, P < 0.05, P < 0.05). Simultaneously, the histological evidence of liver hemorrhage, polymorphonuclear neutrophil infiltration and the overexpression of ICAM-1 and E-selectin in the liver tissue were observed, all of which were attenuated in the VnA pretreated groups.CONCLUSION: The results demonstrate that VnA pretreatment exerts significant protection against hepatic I/R injury in rats. The protective effects are possibly associated with enhancement of antioxidant capacity, reduction of inflammatory responses and suppressed expression of ICAM-1 and E-selectin.
文摘Objective To investigate the anti-oxidant and anti-inflammatory effects of ethanol extract of Polygala sibirica L.var megalopha Fr.(EEP)on RAW264.7 mouse macrophages.Methods RAW264.7 cells were pretreated with 0–200µg/mL EEP or vehicle for 2 h prior to exposure to 1µg/mL lipopolysaccharide(LPS)for 24 h.Nitric oxide(NO)and prostaglandin(PGE2)production were determined by Griess reagent and enzyme-linked immunosorbent assay(ELISA),respectively.The mRNA levels of inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),tumor necrosis factorα(TNF-α),interleukin-1beta(IL-1β),and IL-6 were determined using reverse transcription polymerase chain reaction(RT-PCR).Western blot assay was used to determine the protein expressions of iNOS,COX-2,phosphorylation of extracellular regulated protein kinases(ERK1/2),c-Jun N-terminal kinase(JNK),inhibitory subunit of nuclear factor Kappa B alpha(IκB-α)and p38.Immunofluorescence was used to observe the nuclear expression of nuclear factor-κB p65(NF-κB p65).Additionally,the anti-oxidant potential of EEP was evaluated by reactive oxygen species(ROS)production and the activities of catalase(CAT)and superoxide dismutase(SOD).The 2,2-diphenyl-1-picrylhydrazyl(DPPH),hydroxyl(OH),superoxide anion(O2−)radical and nitrite scavenging activity were also measured.Results The total polyphenol and flavonoid contents of EEP were 23.50±2.16 mg gallic acid equivalent/100 g and 43.78±3.81 mg rutin equivalent/100 g.With EEP treatment(100 and 150µg/mL),there was a notable decrease in NO and PGE2 production induced by LPS in RAW264.7 cells by downregulation of iNOS and COX-2 mRNA and protein expressions(P<0.01 or P<0.05).Furthermore,with EEP treatment(150µg/mL),there was a decrease in the mRNA expression levels of TNF-α,IL-1βand IL-6,as well as in the phosphorylation of ERK,JNK and p38 mitogen-activated protein kinase(MAPK,P<0.01 or P<0.05),by blocking the nuclear translocation of NF-κB p65 in LPS-stimulated cells.In addition,EEP(100 and 150µg/mL)led to an increase in the anti-oxidant enzymes activity of SOD and CAT,with a concomitant decrease in ROS production(P<0.01 or P<0.05).EEP also indicated the DPPH,OH,O2−radical and nitrite scavenging activity.Conclusion EEP inhibited inflammatory responses in activated macrophages through blocking MAPK/NF-κB pathway and protected against oxidative stress.
基金supported by the Guangzhou Science and Technology Program in 2020-Basic and Applied Basic Research Project(Grant No.202002030226)the National Natural Science Foundation of China(Grant No.82174043)the Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515011697).
文摘Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory properties and can be used to treat acute lung injury and eczema.The aim of this study is to solve the problems of extraction and purification of active components from PAF. Materials and Methods: The solvent to be used for extraction and its concentration, the solid-to-liquid ratio, and extraction duration were investigated using a single-factor experiment.An orthogonal design(L_(9)[3^(4)]) was used to determine the optimum extraction conditions. After optimization, the sample’s concentrations and flow velocity, the eluents and their velocity, adsorption time, and the removed water volume were measured. The content of the five steroids in the sample was determined by high-performance liquid chromatography(HPLC). We also investigated the anti-inflammatory property of PAF calyxes before and after purification. Results: The optimum extraction and purification processes were determined by single-factor analysis.AB-8 was identified as the best macroporous adsorption resin for enrichment. After optimization, the average total steroid content was 71.83%,and the average recovery was 90% after purification. Among the five steroid components detected by HPLC, physalin F showed the highest content. Furthermore, the sample obtained after purification could significantly inhibit paw edema by egg whites induced. Conclusions: An environmentally-sustainable, efficient, and stable process was first optimized for enriching and purifying total steroids from PAF. The process has the potential for further development and utilization in the pharmaceutical industry.
文摘为进一步探明陕西省榆林市樟子松人工林的土壤养分变化特征,以5年生樟子松(Pinus sylvestris L. var. mongolica Litv.)纯林和5年生樟子松-胡枝子(Lespedeza bicolor Turcz.)混交林为研究对象,分析土壤有机质、硝态氮、有效磷、速效钾等理化指标以及土壤酶活性的变化特征。结果表明,樟子松纯林和樟子松-胡枝子混交林土壤有机质、有效磷和速效钾含量整体上均随着土层深度的增加呈下降趋势,全磷含量均随着土层深度的增加呈先升高后降低的趋势,硝态氮含量则均随着土壤深度的增加而增加,5个指标均表现为樟子松-胡枝子混交林高于樟子松纯林,且樟子松-胡枝子混交林各层土壤有效磷含量均显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤含水率各土层间差异均不显著,樟子松-胡枝子混交林5~15 cm土层土壤含水率含量显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤碱性磷酸酶活性、过氧化氢酶活性、脲酶活性均随着土层深度的增加而降低,且樟子松-胡枝子混交林过氧化氢酶活性和脲酶活性在各土层均显著高于樟子松纯林(P<0.05)。
基金supported by the Federal Office for Agriculture and Food(BLE)of Germany[Grant No.2816DOKI07(Carcauli)]。
文摘Carotenoids and chlorophylls are among the most widely distributed pigments in nature that play essential roles in the photosynthetic apparatus and confer diverse colours in plants.Among all vegetables,cauliflower(Brassica oleracea L.ssp.var.botrytis)is rich in phytochemicals and is an important crop grown all over the world.This study investigates carotenoid and chlorophyll concentrations in differently pigmented cultivars and elucidates the role of transcriptional regulation of carotenoid accumulation including lutein andβ-carotene.Here,we characterised changes in pigments by UHPLC-DAD-ToF-MS and changes in transcript levels of carotenoid metabolic genes by qRT-PCR in florets and leaves of orange(‘Jaffa'and‘Sunset'),purple(‘Di Sicilia Violetto'and‘Graffiti'),green(‘Trevi')and white(‘Clapton')cultivars.Transcript levels of all carotenoid metabolic genes showed different transcript level patterns in the leaves and florets.Compared to the other cultivars,the orange cultivars had the highest levels ofβ-carotene in the florets and lutein in the leaves resulting in changes lutein/β-carotene ratios.In the green cultivar,higher transcript levels were also found,especially for phytoene synthase and phytoene desaturase genes of the core biosynthesis pathway.However,no increased carotenoid concentrations were observed,possibly due to a higher carotenoid turnover induced by the carotenoid cleavage dioxygenase 4 in the green cultivar.In the white(‘Clapton')and purple(‘Di Sicilia Violetto'and‘Graffiti')cultivars the phytoene desaturase transcript levels as well as carotenoid concentrations were low.Chlorophyll concentrations changed in trend comparable to the carotenoid concentrations and were only significantly lower in the leaves of the orange cultivar‘Jaffa'.Also,the chlorophyll a/b ratio changed in‘Jaffa'.In florets the highest chlorophylls concentrations were observed for the green cultivar(‘Trevi')and the purple cultivar(‘Di Sicilia Violetto').Taken together,the study demonstrates the complex source-sink relationship of carotenoid accumulation in different coloured cauliflower.
基金Supported by Ningxia Natural Science Foundation,No.2022AAC02039Ningxia Key Research and Development Program,No.2023BEG02015National Natural Science Foundation of China,No.82260879.
文摘Coix seed is a dry and mature seed of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf in the Gramineae family.Coix seed has a sweet,light taste,and a cool nature.Coix seed enters the spleen,stomach,and lung meridians.It has the effects of promoting diuresis and dampness,strengthening the spleen to prevent diarrhea,removing arthralgia,expelling pus,and detoxifying and dispersing nodules.It is used for the treatment of edema,athlete's foot,poor urination,spleen deficiency and diarrhea,dampness and obstruction,lung carbuncle,intestinal carbuncle,verruca,and cancer.The medicinal and health value is high,and it has been included in the list of medicinal and food sources in China,which has a large development and application space.This article reviews the current research achievements in the processing methods and anti-tumor activities of Coix seed and provides examples of its clinical application in ancient and modern times,aiming to provide reference for further research on Coix seed and contribute to its clinical application and development.Through the analysis of the traditional Chinese patent medicines,and simple preparations and related health food of Coix seed queried by Yaozhi.com,the source,function,and dosage form of Coix seed were comprehensively analyzed,with a view of providing a reference for the development of Coix seed medicine and food.