目的:通过测定BCL2-L12在正常子宫内膜组织和子宫内膜癌组织中的表达情况,探讨BCL2-L12在子宫内膜癌中的表达及其与病理参数的相关性。方法:应用Real time PCR法检测36例良性病变切除子宫患者(对照组)的正常内膜组织及64例子宫内膜癌患...目的:通过测定BCL2-L12在正常子宫内膜组织和子宫内膜癌组织中的表达情况,探讨BCL2-L12在子宫内膜癌中的表达及其与病理参数的相关性。方法:应用Real time PCR法检测36例良性病变切除子宫患者(对照组)的正常内膜组织及64例子宫内膜癌患者(实验组)病变内膜组织中BCL2-L12的表达情况,并分析其与临床病理参数的关系。结果:BCL2-L12在子宫内膜癌组织中的表达低于正常子宫内膜组织,差异有统计学意义(P<0.05);BCL2-L12在子宫内膜癌组织中的表达水平与肌层浸润程度有关,差异有统计学意义(P<0.05)。BCL2-L12在子宫内膜癌组织中的表达与其他临床病理特征(比如病理学分期、组织学分化程度等)差异无统计学意义(P>0.05)。结论:BCL2-L12在子宫内膜癌组织中呈现低表达,且表达水平与肌层浸润程度有关,提示BCL2-L12可能在子宫内膜癌的发生发展中起抑制作用。展开更多
目的·分析线粒体核糖体蛋白L12(mitochondrial ribosomal protein L12,MRPL12)在肺腺癌(lung adenocarcinoma,LUAD)和正常组织中的表达差异和对患者预后的影响,以及预测其生物学功能。方法·利用肿瘤浸润性免疫细胞分析数据库T...目的·分析线粒体核糖体蛋白L12(mitochondrial ribosomal protein L12,MRPL12)在肺腺癌(lung adenocarcinoma,LUAD)和正常组织中的表达差异和对患者预后的影响,以及预测其生物学功能。方法·利用肿瘤浸润性免疫细胞分析数据库TIMER和GEPIA(gene expression profiling and interactive analyses)交互式网站服务器分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)中MRPL12在不同癌组织中的表达情况的影响。利用Sangerbox生物信息分析平台对MRPL12差异表达的癌症类型进行Cox回归和Kaplan-Meier生存分析,评估风险比(hazard ratio,HR)、95%置信区间(confidence interval,CI)和Log-rank P值。采用UALCAN数据分析平台评估MRPL12蛋白表达与临床病理参数的关系。使用LinkedOmics数据库筛选与MRPL12表达相关的基因。采用DAVID数据库进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。定量聚合酶链反应验证MRPL12在细胞中的转录水平。结果·MRPL12在多种癌组织中显著高表达,但只有在LUAD中MRPL12的高表达与患者较差的总体生存期(overall suivival,OS)和疾病特异性生存期(disease-specific survival,DSS)相关;肿瘤分级程度越高的LUAD患者MRPL12蛋白表达水平越高;在LUAD基因表达数据集中与MRPL12负相关的基因富集到癌症通路(P=0.000)、癌症蛋白聚糖(P=0.000)、非小细胞肺癌(P=0.000)等癌症相关的通路。MRPL12在LUAD细胞(H1395、H1975和HCC827)中的mRNA表达水平高于正常细胞(Beas-2B),差异有统计学意义(P<0.05)。结论·MRPL12高表达的LUAD患者可能预后较差;MRPL12可以作为LUAD预后不良的潜在生物标志物和潜在的治疗新靶点。展开更多
Nitrate transporters(NRTs)are regulators of nitrate assimilation and transport.The genome sequences of TaNRT2L12-A,-B and-D were cloned from wheat(Triticum aestivum L.),and polymorphisms were analyzed by sequencing.Ta...Nitrate transporters(NRTs)are regulators of nitrate assimilation and transport.The genome sequences of TaNRT2L12-A,-B and-D were cloned from wheat(Triticum aestivum L.),and polymorphisms were analyzed by sequencing.TaNRT2L12-D in a germplasm population was highly conserved.However,38 single nucleotide polymorphisms(SNPs)in TaNRT2L12-A coding region and 11 SNPs in TaNRT2L12-B coding region were detected.Two derived cleaved amplified polymorphic sequences(dCAPS)markers A-CSNP1 and A-CSNP2 were developed for TaNRT2L12-A based on SNP-351 and SNP-729,and three haplotypes were identified in the germplasm population.B-CSNP1 and B-CSNP2 were developed for TaNRT2L12-B based on SNP-237 and SNP-1227,and three haplotypes were detected in the germplasm population.Association analyses between the markers and agronomic traits in 30 environments and phenotypic comparisons revealed that A-CSNP2-A is a superior allele of shorter plant height(PH),length of penultimate internode(LPI)and peduncle length(PL),B-CSNP2-G is a superior allele of higher grain number per spike(GNS).Hap-6B-1 containing both superior alleles B-CSNP1-C and B-CSNP2-A is a superior haplotype of 1 OOO-grain weight(TGW).Expression analysis showed that TaNRT2L12-B is mainly expressed in the root base and regulated by nitrate.Therefore,TaNRT2L12 may be involved in nitrate transport and signaling to regulate TGW in wheat.The superior alleles and dCAPS markers of TaNRT2L12-A/B are beneficial to genetic improvement and germplasm enhancement with molecular markers-assisted selection.展开更多
文摘目的:通过测定BCL2-L12在正常子宫内膜组织和子宫内膜癌组织中的表达情况,探讨BCL2-L12在子宫内膜癌中的表达及其与病理参数的相关性。方法:应用Real time PCR法检测36例良性病变切除子宫患者(对照组)的正常内膜组织及64例子宫内膜癌患者(实验组)病变内膜组织中BCL2-L12的表达情况,并分析其与临床病理参数的关系。结果:BCL2-L12在子宫内膜癌组织中的表达低于正常子宫内膜组织,差异有统计学意义(P<0.05);BCL2-L12在子宫内膜癌组织中的表达水平与肌层浸润程度有关,差异有统计学意义(P<0.05)。BCL2-L12在子宫内膜癌组织中的表达与其他临床病理特征(比如病理学分期、组织学分化程度等)差异无统计学意义(P>0.05)。结论:BCL2-L12在子宫内膜癌组织中呈现低表达,且表达水平与肌层浸润程度有关,提示BCL2-L12可能在子宫内膜癌的发生发展中起抑制作用。
文摘目的·分析线粒体核糖体蛋白L12(mitochondrial ribosomal protein L12,MRPL12)在肺腺癌(lung adenocarcinoma,LUAD)和正常组织中的表达差异和对患者预后的影响,以及预测其生物学功能。方法·利用肿瘤浸润性免疫细胞分析数据库TIMER和GEPIA(gene expression profiling and interactive analyses)交互式网站服务器分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)中MRPL12在不同癌组织中的表达情况的影响。利用Sangerbox生物信息分析平台对MRPL12差异表达的癌症类型进行Cox回归和Kaplan-Meier生存分析,评估风险比(hazard ratio,HR)、95%置信区间(confidence interval,CI)和Log-rank P值。采用UALCAN数据分析平台评估MRPL12蛋白表达与临床病理参数的关系。使用LinkedOmics数据库筛选与MRPL12表达相关的基因。采用DAVID数据库进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。定量聚合酶链反应验证MRPL12在细胞中的转录水平。结果·MRPL12在多种癌组织中显著高表达,但只有在LUAD中MRPL12的高表达与患者较差的总体生存期(overall suivival,OS)和疾病特异性生存期(disease-specific survival,DSS)相关;肿瘤分级程度越高的LUAD患者MRPL12蛋白表达水平越高;在LUAD基因表达数据集中与MRPL12负相关的基因富集到癌症通路(P=0.000)、癌症蛋白聚糖(P=0.000)、非小细胞肺癌(P=0.000)等癌症相关的通路。MRPL12在LUAD细胞(H1395、H1975和HCC827)中的mRNA表达水平高于正常细胞(Beas-2B),差异有统计学意义(P<0.05)。结论·MRPL12高表达的LUAD患者可能预后较差;MRPL12可以作为LUAD预后不良的潜在生物标志物和潜在的治疗新靶点。
基金This work was supported by the National Key Research and Development Program of China(2017YFD0300202)and the Agricultural Science and Tech no logy Inno vati on Program of Chinese Academy of Agricultural Sciences(ASTIP).
文摘Nitrate transporters(NRTs)are regulators of nitrate assimilation and transport.The genome sequences of TaNRT2L12-A,-B and-D were cloned from wheat(Triticum aestivum L.),and polymorphisms were analyzed by sequencing.TaNRT2L12-D in a germplasm population was highly conserved.However,38 single nucleotide polymorphisms(SNPs)in TaNRT2L12-A coding region and 11 SNPs in TaNRT2L12-B coding region were detected.Two derived cleaved amplified polymorphic sequences(dCAPS)markers A-CSNP1 and A-CSNP2 were developed for TaNRT2L12-A based on SNP-351 and SNP-729,and three haplotypes were identified in the germplasm population.B-CSNP1 and B-CSNP2 were developed for TaNRT2L12-B based on SNP-237 and SNP-1227,and three haplotypes were detected in the germplasm population.Association analyses between the markers and agronomic traits in 30 environments and phenotypic comparisons revealed that A-CSNP2-A is a superior allele of shorter plant height(PH),length of penultimate internode(LPI)and peduncle length(PL),B-CSNP2-G is a superior allele of higher grain number per spike(GNS).Hap-6B-1 containing both superior alleles B-CSNP1-C and B-CSNP2-A is a superior haplotype of 1 OOO-grain weight(TGW).Expression analysis showed that TaNRT2L12-B is mainly expressed in the root base and regulated by nitrate.Therefore,TaNRT2L12 may be involved in nitrate transport and signaling to regulate TGW in wheat.The superior alleles and dCAPS markers of TaNRT2L12-A/B are beneficial to genetic improvement and germplasm enhancement with molecular markers-assisted selection.
