Postoperative cognitive dysfunction is a seve re complication of the central nervous system that occurs after anesthesia and surgery,and has received attention for its high incidence and effect on the quality of life ...Postoperative cognitive dysfunction is a seve re complication of the central nervous system that occurs after anesthesia and surgery,and has received attention for its high incidence and effect on the quality of life of patients.To date,there are no viable treatment options for postoperative cognitive dysfunction.The identification of postoperative cognitive dysfunction hub genes could provide new research directions and therapeutic targets for future research.To identify the signaling mechanisms contributing to postoperative cognitive dysfunction,we first conducted Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the Gene Expression Omnibus GSE95426 dataset,which consists of mRNAs and long non-coding RNAs differentially expressed in mouse hippocampus3 days after tibial fracture.The dataset was enriched in genes associated with the biological process"regulation of immune cells,"of which Chill was identified as a hub gene.Therefore,we investigated the contribution of chitinase-3-like protein 1 protein expression changes to postoperative cognitive dysfunction in the mouse model of tibial fractu re surgery.Mice were intraperitoneally injected with vehicle or recombinant chitinase-3-like protein 124 hours post-surgery,and the injection groups were compared with untreated control mice for learning and memory capacities using the Y-maze and fear conditioning tests.In addition,protein expression levels of proinflammatory factors(interleukin-1βand inducible nitric oxide synthase),M2-type macrophage markers(CD206 and arginase-1),and cognition-related proteins(brain-derived neurotropic factor and phosphorylated NMDA receptor subunit NR2B)were measured in hippocampus by western blotting.Treatment with recombinant chitinase-3-like protein 1 prevented surgery-induced cognitive impairment,downregulated interleukin-1βand nducible nitric oxide synthase expression,and upregulated CD206,arginase-1,pNR2B,and brain-derived neurotropic factor expression compared with vehicle treatment.Intraperitoneal administration of the specific ERK inhibitor PD98059 diminished the effects of recombinant chitinase-3-like protein 1.Collectively,our findings suggest that recombinant chitinase-3-like protein 1 ameliorates surgery-induced cognitive decline by attenuating neuroinflammation via M2 microglial polarization in the hippocampus.Therefore,recombinant chitinase-3-like protein1 may have therapeutic potential fo r postoperative cognitive dysfunction.展开更多
The present study investigates the effects of congruency and frequency on adjective-noun collocational processing for Chinese learners of English at two proficiency levels based on the data obtained in an online accep...The present study investigates the effects of congruency and frequency on adjective-noun collocational processing for Chinese learners of English at two proficiency levels based on the data obtained in an online acceptability judgment task.The subject pool of this research included 60 English majors studying at a university in China;30 were selected as a higher-proficiency group and 30 as a lower-proficiency group according to their Vocabulary Levels Test(Schmitt et al.,2001)scores and their self-reported proficiency in English.The experimental materials were programmed to E-prime 2.0 and included six types of collocations:(1)15 high-frequency congruent collocations,(2)15 low-frequency congruent collocations,(3)15 high-frequency incongruent collocations,(4)15 low-frequency incongruent collocations,(5)15 Chinese-only items,and(6)75 unrelated items for baseline data.The collected response times(RTs)and accuracy rates data were statistically analyzed by the use of an ANOVA test and pairwise comparisons through SPSS 16.0 software.The results revealed that:(1)the adjective-noun collocational processing of Chinese English learners is influenced by collocational frequency,congruency and L2 proficiency;(2)the processing time is affected by the interaction of congruency and frequency;and(3)the interactive effect of L2 proficiency in conjunction with congruency and frequency also influences the processing quality.展开更多
胆囊癌是胆道系统恶性肿瘤,患者会出现黄疸、腹痛等症状,还会出现胆囊炎和胆囊穿孔等[1,2]。胆囊癌在早期会对邻近周围器官肝脏进行侵犯,不适合进行根治性手术,而且患者易产生化疗耐药性,导致预后不良[3,4]。因此在早期诊断胆囊癌尤为...胆囊癌是胆道系统恶性肿瘤,患者会出现黄疸、腹痛等症状,还会出现胆囊炎和胆囊穿孔等[1,2]。胆囊癌在早期会对邻近周围器官肝脏进行侵犯,不适合进行根治性手术,而且患者易产生化疗耐药性,导致预后不良[3,4]。因此在早期诊断胆囊癌尤为重要。多层螺旋CT(multi-slice spiral CT,MSCT)能提高早期诊断恶性肿瘤准确率,但也在影像学图像判断上存在局限[5]。壳多糖酶3样蛋白1(chitosan enzyme 3-like protein 1,CHI3L1)可参与机体炎症以及血管形成[6]。CHI3L1在多种癌症中呈现高表达[7]。血管生成素样蛋白2(angiopoietin-like protein 2,ANGPTL2)作为癌症治疗的靶点,可参与乳腺癌进展[8]。目前关于MSCT联合血清CHI3L1、ANGPTL2在胆囊癌中的研究鲜有报道,因此,本研究旨在探讨MSCT联合血清CHI3L1、ANGPTL2对胆囊癌的诊断价值。展开更多
首次采用反相高效液相色谱法测定茜草根中1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量。色谱柱为Purospher star RP C18色谱柱(250mm×4.6mm,5μm),流动相...首次采用反相高效液相色谱法测定茜草根中1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量。色谱柱为Purospher star RP C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇:水:四氢呋喃(65:34.7:0.3),流速为1.0mL/min,检测波长为276nm,柱温为25℃。该方法的线性范围为0.020~0.160μg,r=0.9998,平均回收率为101.5%,RSD为2.0%(n=6)。该方法测定1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量灵敏、准确、重现性好。展开更多
采用GNS3网络模拟器结合Vmware虚拟机实现对L2TPv3 over IPSec的仿真配置,解决跨地理位置的多个站点间的安全访问,分析L2TPv3 over IPSec相比于其他VPN的优势,通过设计和配置实验可以更好的掌握理论知识、提升实践能力,进而说明GNS3结合...采用GNS3网络模拟器结合Vmware虚拟机实现对L2TPv3 over IPSec的仿真配置,解决跨地理位置的多个站点间的安全访问,分析L2TPv3 over IPSec相比于其他VPN的优势,通过设计和配置实验可以更好的掌握理论知识、提升实践能力,进而说明GNS3结合Vmware的应用价值。展开更多
基金supported by the National Natural Science Foundation of China,Nos.81730033,82171193(to XG)the Key Talent Project for Strengthening Health during the 13^(th)Five-Year Plan Period,No.ZDRCA2016069(to XG)+1 种基金the National Key R&D Program of China,No.2018YFC2001901(to XG)Jiangsu Provincial Medical Key Discipline,No.ZDXK202232(to XG)。
文摘Postoperative cognitive dysfunction is a seve re complication of the central nervous system that occurs after anesthesia and surgery,and has received attention for its high incidence and effect on the quality of life of patients.To date,there are no viable treatment options for postoperative cognitive dysfunction.The identification of postoperative cognitive dysfunction hub genes could provide new research directions and therapeutic targets for future research.To identify the signaling mechanisms contributing to postoperative cognitive dysfunction,we first conducted Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the Gene Expression Omnibus GSE95426 dataset,which consists of mRNAs and long non-coding RNAs differentially expressed in mouse hippocampus3 days after tibial fracture.The dataset was enriched in genes associated with the biological process"regulation of immune cells,"of which Chill was identified as a hub gene.Therefore,we investigated the contribution of chitinase-3-like protein 1 protein expression changes to postoperative cognitive dysfunction in the mouse model of tibial fractu re surgery.Mice were intraperitoneally injected with vehicle or recombinant chitinase-3-like protein 124 hours post-surgery,and the injection groups were compared with untreated control mice for learning and memory capacities using the Y-maze and fear conditioning tests.In addition,protein expression levels of proinflammatory factors(interleukin-1βand inducible nitric oxide synthase),M2-type macrophage markers(CD206 and arginase-1),and cognition-related proteins(brain-derived neurotropic factor and phosphorylated NMDA receptor subunit NR2B)were measured in hippocampus by western blotting.Treatment with recombinant chitinase-3-like protein 1 prevented surgery-induced cognitive impairment,downregulated interleukin-1βand nducible nitric oxide synthase expression,and upregulated CD206,arginase-1,pNR2B,and brain-derived neurotropic factor expression compared with vehicle treatment.Intraperitoneal administration of the specific ERK inhibitor PD98059 diminished the effects of recombinant chitinase-3-like protein 1.Collectively,our findings suggest that recombinant chitinase-3-like protein 1 ameliorates surgery-induced cognitive decline by attenuating neuroinflammation via M2 microglial polarization in the hippocampus.Therefore,recombinant chitinase-3-like protein1 may have therapeutic potential fo r postoperative cognitive dysfunction.
文摘The present study investigates the effects of congruency and frequency on adjective-noun collocational processing for Chinese learners of English at two proficiency levels based on the data obtained in an online acceptability judgment task.The subject pool of this research included 60 English majors studying at a university in China;30 were selected as a higher-proficiency group and 30 as a lower-proficiency group according to their Vocabulary Levels Test(Schmitt et al.,2001)scores and their self-reported proficiency in English.The experimental materials were programmed to E-prime 2.0 and included six types of collocations:(1)15 high-frequency congruent collocations,(2)15 low-frequency congruent collocations,(3)15 high-frequency incongruent collocations,(4)15 low-frequency incongruent collocations,(5)15 Chinese-only items,and(6)75 unrelated items for baseline data.The collected response times(RTs)and accuracy rates data were statistically analyzed by the use of an ANOVA test and pairwise comparisons through SPSS 16.0 software.The results revealed that:(1)the adjective-noun collocational processing of Chinese English learners is influenced by collocational frequency,congruency and L2 proficiency;(2)the processing time is affected by the interaction of congruency and frequency;and(3)the interactive effect of L2 proficiency in conjunction with congruency and frequency also influences the processing quality.
文摘胆囊癌是胆道系统恶性肿瘤,患者会出现黄疸、腹痛等症状,还会出现胆囊炎和胆囊穿孔等[1,2]。胆囊癌在早期会对邻近周围器官肝脏进行侵犯,不适合进行根治性手术,而且患者易产生化疗耐药性,导致预后不良[3,4]。因此在早期诊断胆囊癌尤为重要。多层螺旋CT(multi-slice spiral CT,MSCT)能提高早期诊断恶性肿瘤准确率,但也在影像学图像判断上存在局限[5]。壳多糖酶3样蛋白1(chitosan enzyme 3-like protein 1,CHI3L1)可参与机体炎症以及血管形成[6]。CHI3L1在多种癌症中呈现高表达[7]。血管生成素样蛋白2(angiopoietin-like protein 2,ANGPTL2)作为癌症治疗的靶点,可参与乳腺癌进展[8]。目前关于MSCT联合血清CHI3L1、ANGPTL2在胆囊癌中的研究鲜有报道,因此,本研究旨在探讨MSCT联合血清CHI3L1、ANGPTL2对胆囊癌的诊断价值。
文摘首次采用反相高效液相色谱法测定茜草根中1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量。色谱柱为Purospher star RP C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇:水:四氢呋喃(65:34.7:0.3),流速为1.0mL/min,检测波长为276nm,柱温为25℃。该方法的线性范围为0.020~0.160μg,r=0.9998,平均回收率为101.5%,RSD为2.0%(n=6)。该方法测定1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量灵敏、准确、重现性好。
