目的探讨CRIF1基因对L615白血病细胞小鼠体内增殖的影响。方法实验分为3组,CRIF1-RNAi L615实验组(n=3):慢病毒干扰载体抑制L615细胞CRIF1-GFP基因表达;空载体对照组(n=3):空载体转染L615细胞;空白对照组(L615细胞空白对照)(n=3);建立...目的探讨CRIF1基因对L615白血病细胞小鼠体内增殖的影响。方法实验分为3组,CRIF1-RNAi L615实验组(n=3):慢病毒干扰载体抑制L615细胞CRIF1-GFP基因表达;空载体对照组(n=3):空载体转染L615细胞;空白对照组(L615细胞空白对照)(n=3);建立白血病小鼠模型。通过肝脾印片、骨髓涂片及外周血流式细胞仪检测观察各组小鼠体内L615细胞增殖情况,比较各组小鼠的外周血白细胞计数(WBC)、生活质量和生存时间、肝脾指数。结果流式细胞仪检测:CRIF1-RNAi L615实验组、空载体对照组的小鼠外周血均见大量GFP阳性L615细胞;肝、脾印片及骨髓涂片结果显示肝、脾、骨髓均被白血病细胞浸润,3组小鼠均成瘤。CRIF1-RNAi L615实验组、空载体对照组及L615对照组小鼠在白血病细胞植入d5后外周血WBC(×109/L)分别为:42.23±15.08 vs 17.10±4.10 vs 17.30±5.05(P<0.01);生存时间(d)分别为:6.33±1.15 vs 10.00 vs 8.33±2.89(P<0.01);CRIF1-RNAi L615实验组小鼠脱毛、弓背等并发症出现提前;肝、脾指数分别为:122.31±2.83、35.21±0.45 vs 110.89±13.30、21.54±3.40 vs 89.76±5.56、24.71±2.48(P<0.05)。结论抑制小鼠L615白血病细胞CRIF1基因表达后,明显著促进L615白血病细胞在小鼠体内增殖、浸润并加快移植小鼠发病死亡。展开更多
A Review] The biological characteristics of viral L6565 leukemia cell clone were as follows: (1) The chromosome counts varied 38～114 , and stem cells were 42; (2) Virus particles type A and type C found in the cytopl...A Review] The biological characteristics of viral L6565 leukemia cell clone were as follows: (1) The chromosome counts varied 38～114 , and stem cells were 42; (2) Virus particles type A and type C found in the cytoplasm of clone cells; (3) X-C assays were positive, c- myc and c- fos gene overexpressed in clone cells; (4) Differential markers CD4, CD8, CD45R were negative, CD45RO λ were positive; (5) The supernatant of clone cells could induce T or B lymphocytic leukemia/lymphoma and granulocytic leukemia in SSB strain mice. The leukemogenic effect of concentrate supernatant was stronger than non-concentrate supernatant( P< 0.05); (6) The antisense of the c- myc gene induced low expression of c- myc protein, and inhibited the growth of viral L6565 clone cells.展开更多
文摘目的探讨CRIF1基因对L615白血病细胞小鼠体内增殖的影响。方法实验分为3组,CRIF1-RNAi L615实验组(n=3):慢病毒干扰载体抑制L615细胞CRIF1-GFP基因表达;空载体对照组(n=3):空载体转染L615细胞;空白对照组(L615细胞空白对照)(n=3);建立白血病小鼠模型。通过肝脾印片、骨髓涂片及外周血流式细胞仪检测观察各组小鼠体内L615细胞增殖情况,比较各组小鼠的外周血白细胞计数(WBC)、生活质量和生存时间、肝脾指数。结果流式细胞仪检测:CRIF1-RNAi L615实验组、空载体对照组的小鼠外周血均见大量GFP阳性L615细胞;肝、脾印片及骨髓涂片结果显示肝、脾、骨髓均被白血病细胞浸润,3组小鼠均成瘤。CRIF1-RNAi L615实验组、空载体对照组及L615对照组小鼠在白血病细胞植入d5后外周血WBC(×109/L)分别为:42.23±15.08 vs 17.10±4.10 vs 17.30±5.05(P<0.01);生存时间(d)分别为:6.33±1.15 vs 10.00 vs 8.33±2.89(P<0.01);CRIF1-RNAi L615实验组小鼠脱毛、弓背等并发症出现提前;肝、脾指数分别为:122.31±2.83、35.21±0.45 vs 110.89±13.30、21.54±3.40 vs 89.76±5.56、24.71±2.48(P<0.05)。结论抑制小鼠L615白血病细胞CRIF1基因表达后,明显著促进L615白血病细胞在小鼠体内增殖、浸润并加快移植小鼠发病死亡。
文摘A Review] The biological characteristics of viral L6565 leukemia cell clone were as follows: (1) The chromosome counts varied 38～114 , and stem cells were 42; (2) Virus particles type A and type C found in the cytoplasm of clone cells; (3) X-C assays were positive, c- myc and c- fos gene overexpressed in clone cells; (4) Differential markers CD4, CD8, CD45R were negative, CD45RO λ were positive; (5) The supernatant of clone cells could induce T or B lymphocytic leukemia/lymphoma and granulocytic leukemia in SSB strain mice. The leukemogenic effect of concentrate supernatant was stronger than non-concentrate supernatant( P< 0.05); (6) The antisense of the c- myc gene induced low expression of c- myc protein, and inhibited the growth of viral L6565 clone cells.
