LC3和lamins在细胞核自噬中的研究进展

自噬是细胞内的重要生理机制之一,对细胞来说自噬是一把双刃剑,它曾一度被认为是机体的保护机制,但随着研究的深入,学者发现自噬也可促进肿瘤的生长.由于细胞核在真核生物中的重要性,相关自噬蛋白在核中的大量分布,核自噬在多种疾病的发生发展中起着非常重要的作用.为了全面了解疾病与自噬的关系,人们开始了核自噬方面的探索.本文对近年来核自噬的研究进展进行文献综述,总结了其研究历史和里程碑式的发现,为核自噬的研究提供了新的思路.

槲皮素对微重力引起骨髓间充质干细胞衰老的影响研究

目的 研究槲皮素对微重力作用下建立的骨髓间充质干细胞衰老模型的影响。方法 用3D回转仪构造骨髓间充质干细胞衰老模型,给予细胞槲皮素后进行相关蛋白检测、氧化指标检测对药物抗衰老作用进行评估研究。结果 与对照组相比微重力组细胞的衰老相关蛋白p21、p16、p53、RB的表达显著性上升、细胞周期蛋白Cyclin D1、核纤层蛋白lamin B1表达显著下降,差异具有统计学意义(P<0.05)。并且微重力组细胞线粒体膜电位明显下降(P<0.05),细胞ROS蓄积量明显上升(P<0.05),SOD含量明显下降(P<0.05),MDA含量明显上升(P<0.05)。相较于微重力组细胞,槲皮素组细胞的衰老相关蛋白p21、p16、p53、RB的表达显著性下降、细胞周期蛋白cyclin D1、核纤层蛋白lamin B1表达显著上升,差异具有统计学意义(P<0.05)。并且槲皮素组细胞线粒体膜电位明显上升(P<0.05),细胞ROS蓄积量明显下降(P<0.05),SOD含量明显上升(P<0.05),MDA含量明显下降(P<0.05)。结论 槲皮素可抗氧化、保护线粒体功能、保护细胞周期正常进行,从而延缓微重力导致的骨髓间充质干细胞衰老。

Effects of Sinusoidal Vibration of Crystallization Roller on Composite Microstructure of Ti/Al Laminated Composites by Twin-Roll Casting

A new,innovative vibration cast-rolling technology of “electromagnetic stirring+dendrite breaking+asynchronous rolling” was proposed with the adoption of sinusoidal vibration of crystallization roller to prepare Ti/Al laminated composites,and the effect of sinusoidal vibration of crystallization roller on composite microstructure was investigated in detail.The results show that the metallurgical bonding of titanium and aluminum is realized by mesh interweaving and mosaic meshing,instead of transition bonding by forming metal compound layer.The meshing depth between titanium and aluminum layers (6.6μm) of cast-rolling materials with strong vibration of crystallization roller (amplitude 0.87 mm,vibration frequency 25 Hz) is doubled compared with that of traditional cast-rolling materials (3.1μm),and the composite interfacial strength(27.0 N/mm) is twice as high as that of traditional cast-rolling materials (14.9 N/mm).This is because with the action of high-speed superposition of strong tension along the rolling direction,strong pressure along the width direction and rolling force,the composite linearity evolves from "straight line" with traditional casting-rolling to "curved line",and the depth and number of cracks in the interface increases greatly compared with those with traditional cast-rolling,which leads to the deep expansion of the meshing area between interfacial layers and promotes the stable enhancement of composite quality.

Theoretical and experimental investigation of the resonance responses and chaotic dynamics of a bistable laminated composite shell in the dynamic snap-through mode

The dynamic model of a bistable laminated composite shell simply supported by four corners is further developed to investigate the resonance responses and chaotic behaviors.The existence of the 1:1 resonance relationship between two order vibration modes of the system is verified.The resonance response of this class of bistable structures in the dynamic snap-through mode is investigated,and the four-dimensional(4D)nonlinear modulation equations are derived based on the 1:1 internal resonance relationship by means of the multiple scales method.The Hopf bifurcation and instability interval of the amplitude frequency and force amplitude curves are analyzed.The discussion focuses on investigating the effects of key parameters,e.g.,excitation amplitude,damping coefficient,and detuning parameters,on the resonance responses.The numerical simulations show that the foundation excitation and the degree of coupling between the vibration modes exert a substantial effect on the chaotic dynamics of the system.Furthermore,the significant motions under particular excitation conditions are visualized by bifurcation diagrams,time histories,phase portraits,three-dimensional(3D)phase portraits,and Poincare maps.Finally,the vibration experiment is carried out to study the amplitude frequency responses and bifurcation characteristics for the bistable laminated composite shell,yielding results that are qualitatively consistent with the theoretical results.

Snap-through behaviors and nonlinear vibrations of a bistable composite laminated cantilever shell:an experimental and numerical study

The snap-through behaviors and nonlinear vibrations are investigated for a bistable composite laminated cantilever shell subjected to transversal foundation excitation based on experimental and theoretical approaches.An improved experimental specimen is designed in order to satisfy the cantilever support boundary condition,which is composed of an asymmetric region and a symmetric region.The symmetric region of the experimental specimen is entirely clamped,which is rigidly connected to an electromagnetic shaker,while the asymmetric region remains free of constraint.Different motion paths are realized for the bistable cantilever shell by changing the input signal levels of the electromagnetic shaker,and the displacement responses of the shell are collected by the laser displacement sensors.The numerical simulation is conducted based on the established theoretical model of the bistable composite laminated cantilever shell,and an off-axis three-dimensional dynamic snap-through domain is obtained.The numerical solutions are in good agreement with the experimental results.The nonlinear stiffness characteristics,dynamic snap-through domain,and chaos and bifurcation behaviors of the shell are quantitatively analyzed.Due to the asymmetry of the boundary condition and the shell,the upper stable-state of the shell exhibits an obvious soft spring stiffness characteristic,and the lower stable-state shows a linear stiffness characteristic of the shell.

Dynamic and electrical responses of a curved sandwich beam with glass reinforced laminate layers and a pliable core in the presence of a piezoelectric layer under low-velocity impact

The dynamic responses and generated voltage in a curved sandwich beam with glass reinforced laminate(GRL)layers and a pliable core in the presence of a piezoelectric layer under low-velocity impact(LVI)are investigated.The current study aims to carry out a dynamic analysis on the sandwich beam when the impactor hits the top face sheet with an initial velocity.For the layer analysis,the high-order shear deformation theory(HSDT)and Frostig's second model for the displacement fields of the core layer are used.The classical non-adhesive elastic contact theory and Hunter's principle are used to calculate the dynamic responses in terms of time.In order to validate the analytical method,the outcomes of the current investigation are compared with those gained by the experimental tests carried out by other researchers for a rectangular composite plate subject to the LVI.Finite element(FE)simulations are conducted by means of the ABAQUS software.The effects of the parameters such as foam modulus,layer material,fiber angle,impactor mass,and its velocity on the generated voltage are reviewed.

Study of damage behavior and repair effectiveness of patch repaired carbon fiber laminate under quasi-static indentation loading

Damage caused due to low-velocity impacts in composites leads to substantial deterioration in their residual strength and eventually provokes structural failure.This work presents an experimental investigation on the effects of different patch and parent laminate stacking sequences on the enhancement of impact strength of Carbon Fiber Reinforced Polymers(CFRP)composites by utilising the adhesively bonded external patch repair technique.Damage evolution study is also performed with the aid of Acoustic Emission(AE).Two different quasi-isotropic configurations were selected for the parent laminate,viz.,[45°/45°/0°/0°]s and[45°/0°/45°/0°]s.Quasi Static Indentation(QSI)test was performed on both the pristine laminates,and damage areas were detected by using the C-scan inspection technique.Damaged laminates were repaired by using a single-sided patch of two different configurations,viz.,[45°/45°/45°/45°]and[45°/0°/0°/45°],and employing a circular plug to fill the damaged hole.Four different combinations of repaired laminates with two configurations of each parent and patch laminate were produced,which were further subjected to the QSI test.The results reveal the effectiveness of the repair method,as all the repaired laminates show higher impact resistance compared to the respective pristine laminates.Patches of[45°/0°/0°/45°]configuration when repaired by taking[45°/45°/0°/0°]s and[45°/0°/45°/0°]s as parents exhibited 68%and 73%higher peak loads,respectively,than the respective pristine laminates.Furthermore,parent and patch of configuration[45°/0°/45°/0°]s and[45°/0°/0°/45°],respectively,attain the highest peak load,whereas[45°/45°/0°/0°]s and[45°/45°/45°/45°]combinations possess the most gradual decrease in the load.

Research on Fatigue Damage Behavior of Main Beam Sub-Structure of Composite Wind Turbine Blade

Given the difficulty in accurately evaluating the fatigue performance of large composite wind turbine blades(referred to as blades),this paper takes the main beam structure of the blade with a rectangular cross-sectionas the simulation object and establishes a composite laminate rectangular beam structure that simultaneouslyincludes the flange,web,and adhesive layer,referred to as the blade main beam sub-structure specimen,throughthe definition of blade sub-structures.This paper examines the progressive damage evolution law of the compositelaminate rectangular beam utilizing an improved 3D Hashin failure criterion,cohesive zone model,B-K failurecriterion,and computer simulation technology.Under static loading,the layup angle of the anti-shear web hasa close relationship with the static load-carrying capacity of the composite laminate rectangular beam;under fatigueloading,the fatigue damage will first occur in the lower flange adhesive area of the whole composite laminaterectangular beam and ultimately result in the fracture failure of the entire structure.These results provide a theoreticalreference and foundation for evaluating and predicting the fatigue performance of the blade main beamstructure and even the full-size blade.

Ballistic penetration damages of hybrid plain-woven laminates with carbon,Kevlar and UHMWPE fibers in different stacking sequences

Hybrid composite materials combine different fibers in preform and take advantages of different mechanical behaviors for improving ballistic impact damage tolerances.Here we report ballistic impact damages of plain-woven laminates with different hybrids and stacking sequences.Three kinds of hybrid laminates,i.e.,carbon/Kevlar,carbon/ultra-high molecular weight polyethylene(UHMWPE),and UHMWPE/Kevlar,had been prepared and tested in ballistic penetration with fragment simulating projectiles(FSP).The residual velocities of the projectiles and impact damage morphologies of the laminates have been obtained to show impact energy absorptions for the different hybrid schemes.A microstructural model of the hybrid laminates had also been established to show impact damage mechanisms with finite element analysis(FEA).We found that the UHMWPE/Kevlar hybrid laminates with Kevlar layers as the front face have the highest energy absorption capacity,followed by the carbon/Kevlar hybrid laminates with carbon layers as the front face.The main damage modes are fiber breakages,matrix crack and interlayer delamination.The ballistic damage evolutions from the FEA results show that the major damage is shear failure for front layers,while tension failure for the back layers.We expect that the ballistic impact performance could be improved from the different hybrid schemes.

Multi-stage penetration characteristics of thick ultra-high molecular weight polyethylene laminates

To further reveal the failure mechanisms of thick ultra-high molecular weight polyethylene(UHMWPE)laminates,field firing tests were conducted for 10-,20-,and 30-mm thick laminates against 12.7-mm calibre wedge-shaped fragment simulated projectiles at high velocities between 450 and 1200 m/s.The ballistic performance,deformation process,and staged failure characteristics of the laminates with different thicknesses were compared and analysed.The results demonstrate that the ballistic limits of the UHMWPE laminates increase almost linearly with laminate thickness.The 10-mm thick laminate generally experiences two-stage failure characteristics,whereas three-staged failure occurs in the 20-and 30-mm thick laminates and the progressive delamination is evident.The energy limit concept representing the maximum energy absorption efficiency and the idea of reuse of the thick UHMWPE laminates are proposed in this study.The findings of this research will be useful in the design of flexible and effective UHMWPE-based protective equipment.

Dynamic analysis of bio-inspired helicoid laminated composite plates resting on Pasternak foundation excited by explosive loading

This paper uses isogeometric analysis(IGA)based on higher-order shear deformation theory(HSDT)to study the dynamic response of bio-inspired helicoid laminated composite(B-iHLC)plates resting on Pasternak foundation(PF)excited by explosive loading.IGA takes advantage of non-uniform rational Bspline(NURBS)basic functions to exactly represent the structure geometry models and the attainment of higher-order approximation conditions.This method also ensures a C1 continuous function in the analysis of transverse shear deformation via HSDT.Furthermore,IGA eliminates the requirement for correction factors and delivers accurate results.Pasternak foundation with two stiffness parameters:springer stiffness(k_(1))and shear stiffness(k_(2)).The derivation of the governing equations is based on Hamilton's principle.The proposed method is validated through numerical examples.A comprehensive analysis of the impact of geometrical parameters,material properties,boundary conditions(BCs),and foundation stiffness on dynamic response of B-i HLC plates is carried out.

Static Analysis of Anisotropic Doubly-Curved Shell Subjected to Concentrated Loads Employing Higher Order Layer-Wise Theories

In the presentmanuscript,a Layer-Wise(LW)generalizedmodel is proposed for the linear static analysis of doublycurved shells constrained with general boundary conditions under the influence of concentrated and surface loads.The unknown field variable is modelled employing polynomials of various orders,each of them defined within each layer of the structure.As a particular case of the LW model,an Equivalent Single Layer(ESL)formulation is derived too.Different approaches are outlined for the assessment of external forces,as well as for non-conventional constraints.The doubly-curved shell is composed by superimposed generally anisotropic laminae,each of them characterized by an arbitrary orientation.The fundamental governing equations are derived starting from an orthogonal set of principal coordinates.Furthermore,generalized blending functions account for the distortion of the physical domain.The implementation of the fundamental governing equations is performed bymeans of the Generalized Differential Quadrature(GDQ)method,whereas the numerical integrations are computed employing theGeneralized IntegralQuadrature(GIQ)method.In the post-processing phase,an effective procedure is adopted for the reconstruction of stress and strain through-the-thickness distributions based on the exact fulfillment of three-dimensional equilibrium equations.A series of systematic investigations are performed in which the static response of structures with various curvatures and lamination schemes,calculated by the present methodology,have been successfully compared to those ones obtained fromrefined finite element three-dimensional simulations.Even though the present LW approach accounts for a two-dimensional assessment of the structural problem,it is capable of well predicting the three-dimensional response of structures with different characteristics,taking into account a reduced computational cost and pretending to be a valid alternative to widespread numerical implementations.

Vibration characteristics of sandwich plates with an auxetic honeycomb core and laminated three-phase skin layers under blast load

In this article,vibration characteristics of sandwich plates with an auxetic honeycomb core and laminated three-phase skin layers subjected to blast load are studied.Higher-order ES-MITC3 element based on higher-order shear deformation theory(HSDT)to achieve the governing equations.The sandwich plates with the ultra-light feature of the auxetic honeycomb core layer(negative Poisson’s ratio)and reinforced by two laminated three-phase skin layers.The obtained results in our work are compared with other previously published to confirm accuracy and reliability.In addition,the effects of parameters such as geometrical and material parameters on the vibration characteristics of sandwich plates with an auxetic honeycomb core and laminated three-phase skin layers are fully investigated.

Effects of Hydrothermal Environment on the Deformation of the Thin Bamboo Bundle Veneer Laminated Composites

To overcome warping in thin bamboo bundle veneer laminated composites(TBLC),their hydrothermal deformation characteristics were systematically investigated in this study.It was found that TBLCs accelerated the release of internal stress in the thickness direction in a hydrothermal environment,which increased their warpage.TBLCs showed increased warpage in the width and diagonal directions upon increasing the temperature.The warpage of Type E increased by 155.88%and 66.67%in the width and diagonal directions,respectively,when the temperature increased from 25
C to 100
C.The symmetrical TBLC with cross-lay-up and odd layers displayed better hydrothermal stability.We revealed that the deformation of the TBLCs could be regulated under the synergistic effect of water and temperature.These results provide a scientific basis for improving the uniformity of bamboo bundle composite materials and for developing thin bamboo bundle fiber composite materials with designable structures and controllable performance.

Three Dimensional Coupling between Elastic and Thermal Fields in the Static Analysis of Multilayered Composite Shells

This new work aims to develop a full coupled thermomechanical method including both the temperature profile and displacements as primary unknowns of the model.This generic full coupled 3D exact shell model permits the thermal stress investigation of laminated isotropic,composite and sandwich structures.Cylindrical and spherical panels,cylinders and plates are analyzed in orthogonal mixed curved reference coordinates.The 3D equilibrium relations and the 3D Fourier heat conduction equation for spherical shells are coupled and they trivially can be simplified in those for plates and cylindrical panels.The exponential matrix methodology is used to find the solutions of a full coupled model based on coupled differential relations with respect to the thickness coordinate.The analytical solution is based on theories of simply supported edges and harmonic relations for displacement components and sovra-temperature.The sovra-temperature magnitudes are directly applied at the outer faces through static state hypotheses.As a consequence,the sovra-temperature description is assumed to be an unknown variable of themodel and it is calculated in the sameway as the three displacements.The final systemis based on a set of coupled homogeneous differential relations of second order in the thickness coordinate.This system is reduced in a first order differential relation system by redoubling the number of unknowns.Therefore,the exponential matrix methodology is applied to calculate the solution.The temperature field effects are evaluated in the static investigation of shells and plates in terms of displacement and stress components.After an appropriate preliminary validation,new benchmarks are discussed for several thickness ratios,geometrical data,lamination sequences,materials and sovra-temperature values imposed at the outer faces.Results make evident the accordance between the uncoupled thermo-mechanical model and this new full coupled thermo-mechanical model without the need to separately solve the Fourier heat conduction relation.Both effects connected with the thickness layer and the related embedded materials are included in the conducted thermal stress analysis.

Investigation of Color Change of Different Restoration Thickness, Background Color and Resin Cement Shade on CAD/CAM Glass Ceramic Materials

The aim of this study is to investigate the color change of different restoration thicknesses, backgrounds and resin cement colors on lithium disilicate and zirconium reinforced lithium silicate materials in vitro. In this study, IPS emax CAD (LT C14) and Celtra Duo (LT C14) are used as full ceramic materials, and Variolink Esthetic LC (warm, neutral) used as resin cement and Tokuyama Estelite Sigma Quick (A3, A2) is used as composite materials. A total of 160 samples in the form of 40 pieces of 5 × 5 0.4 mm thick 40 pieces of 5 × 5 0.6 mm thick square discs from each of the all-ceramic materials in block form were obtained using a water jet device (DWJ1525-FA;Dardi International Corporation, Nanjing, China). Glass ceramic samples produced in 2 different thicknesses were cemented on 2 different backgrounds with 2 different resin types of cement. Color measurements of the samples before and after cementation were performed on a grey background with spectrophotometer Vita EasyShade V (Vita Zahnfabrik, Bad Sackingen, Germany) and color parameters (L*, a*, b*, ΔE) were calculated according to the CIE Lab (Commission Internationale de L’Eclairage) system. Average values for each group (ΔE) were not affected by ceramic type, material thickness, background color, resin cement color, and the interaction of these four variables (p > 0.05). When the triple interactions between the groups were examined, there were no statistically significant differences (p > 0.05). In the evaluation of pairwise interactions between two groups (material type-material thickness, material type-background color, and thickness of material-background interactions) statistically significant differences (p Implications: The material type, thickness, background and cement color used did not cause any statistically significant color change in lithium disilicate and zirconium-reinforced lithium silicate glass ceramic materials (p > 0.05).

Investigation of Various Laminating Materials for Interior Permanent Magnet Brushless DC Motor for Cooling Fan Application

Permanent magnet brushless DC motors are used for various low-power applications,namely domestic fans,washing machines,mixer grinders and cooling fan applications.This paper focuses on selecting the best laminating material for the interior permanent magnet brushless DC(IPM BLDC)motor used in the cooling fan of automobiles.Various laminating materials,namely M19-29GA,M800-65A and M43,are tested using finite element analysis.The machine's vital performance metrics,namely the stator current,torque ripple,and hysteresis loss were analyzed in selecting the laminating material.The designed motor is also modelled as a mathematical model from the computed lumped parameters.The performance of the machines was validated through electromagnetic and thermal analysis.

Effect of Molding Technique That Move Model Position Just before Formation in Production of Laminated Mouthguard

Many molding techniques have been researched to ensure the thickness of custom mouthguards. The aim of this study was to clarify the effect on the thickness of a laminated mouthguard of a molding technique in which the model position is moved forward just before molding. Mouthguards were molded using a 3.0-mm-thick ethylene vinyl acetate mouthguard sheet and a pressure molding machine. The molding method was the normal molding method (condition C) and the molding technique (condition MP) in which the model position was moved 20 mm forward just before molding. Regarding the molding of the first layer (F) and the second layer (S), the following four molding methods based on the combination of conditions C and MP were compared;FC-SC, FC-SMP, FMP-SC, and FMP-SMP. Differences in mouthguard thickness due to molding conditions for the first and second layers were analyzed by two-way ANOVA and Bonferroni’s multiple comparison test. Significant differences were observed among all molding conditions on the labial surface, and the thicknesses were in the order FC-SC < FC-SMP < FMP-SC < FMP-SMP. FMP-SMP was 4.67 mm thick, which was 1.39 mm thicker than FC-SC. FC-SC was the thinnest at the cusp, and a significant difference was observed between other molding conditions. On the buccal side, significant differences were observed between all conditions except FC-SMP and FMP-SC, and the thicknesses were in the order FC-SC < FC-SMP, FMP-SC < FMP-SMP. The results of this study suggested that the labial and buccal sides of laminated mouthguards could be made 1.4 and 1.2 times thicker when a molding technique that moves the model position just before formation was used for the first and second layers. The reduction in thickness was suppressed by approximately 23.2% and approximately 10.7% on the labial and buccal sides, respectively, compared with the normal molding method.

石杉碱甲对D-半乳糖致衰老大鼠海马及下丘Lamin A／C、prelamin A及FACE1表达的影响

目的研究D-半乳糖致衰老大鼠海马及下丘laminA／C、prelaminA、FACEl的水平变化，并进-步明确石杉碱甲对D-半乳糖诱导的这些蛋白变化的影响及其意义。方法36只健康雄性SD大鼠随机分成对照组、D-半乳糖治疗组和D-半乳糖联合石杉碱甲组。D-半乳糖组每天皮下注射D-半乳糖300mg／Kg，共8W；D-半乳糖联合石杉碱甲组每天D-半乳糖300mg／Kg联合石杉碱甲0．1mg／Kg共同皮下注射，共8W。对照组皮下注射等量生理盐水。应用蛋白免疫印迹杂交（Western blotting）方法检测3组大鼠海马、下丘laminA／C、prelaminA和FACEl的表达。结果海马中，laminA在对照组、D半乳糖＋石杉碱甲组、D-半乳糖组中逐渐降低，laminC水平逐渐升高，prelaminA在对照组中最高，D-半乳糖组与D-半乳糖＋石杉碱甲组无明显差异；FACEl在D-半乳糖＋石杉碱甲组水平最高，对照组中水平最低。下丘中，laminA水平在D-半乳糖＋石杉碱甲组最高，D-半乳糖组中最低；D-半乳糖组和D-半乳糖＋石杉碱甲组laminC水平较对照组升高，D-半乳糖组与D-半乳糖＋石杉碱甲组之间的差异无统计学意义；prelaminA水平在对照组中最低，D-半乳糖＋石杉碱甲组中最高；FACE1水平在对照组、D-半乳糖＋石杉碱甲组、D-半乳糖组中逐渐升高。结论LaminA降低、laminC及FACE1升高预示着D-半乳糖诱导的海马及下丘衰老。石杉碱甲可以在-定程度上改善部分指标的变化，说明它有-定的延缓海马及下丘衰老的作用。