With the implementation of the Official Mexican Standard NOM-011-CONAGUA-2000 [1], the water balance of 730 basins has been calculated and its water availability agreement is published. This rule points out to allocat...With the implementation of the Official Mexican Standard NOM-011-CONAGUA-2000 [1], the water balance of 730 basins has been calculated and its water availability agreement is published. This rule points out to allocate water for the environment only as an annual volume since methods for estimating environmental flows were not standardized in the country. For this reason, The Water Agency (CONAGUA) issued the standard NMX-AA-159-SCFI-2012 [2], to assess environmental flows needed both, at the strategic level in Integrated Water Resources Management (IWRM), or as part of the Environmental Impact Assessment (EIA) of large hydraulic projects. For over ten years, this standard was developed and finally published in September 2012 [3]. It explains different methods from hydrological to holistic approaches, with examples for the country. Its application will cover the urgent need to preserve water for ecosystems in watersheds with high ecological importance and low stress for water use. In this paper, an analysis of the environmental flow standard and examples of the suggested hydrological methods are presented. For its implementation, some steps are taking place, mainly establishing environmental water reserves and building capacities. In addition, environmental allocations are becoming a common practice for all water projects, as well as setting limits to hydrological alterations by hydroelectric dams. The standard promotes the use of technical integration tools to analyze the responses of ecosystems to changes in the flow regime and adaptive management under different scenarios of water use. Although the main steps have been taken, its implementation as mandatory rule will take time.展开更多
There is an urgent need for developing a procedure for biomarker standardization and relative quantificationin clinical laboratories. Measuring the expression levels of cell antigens is critical for the diagnosis of m...There is an urgent need for developing a procedure for biomarker standardization and relative quantificationin clinical laboratories. Measuring the expression levels of cell antigens is critical for the diagnosis of many diseases, e.g. leukemia, lymphoma and immunodeficiency diseases. One of the most significant challenges in flow cytometry is obtaining inter-laboratory and intra-laboratory consistent and reproducible results across multiple cytometer platforms and locations longitudinally over time. To obtain measurement consistency, the target flow cytometer voltages should be optimized to segregate the negative population from the electronic noise, and to keep the brightest positive population within the dynamic range of each detector. Then target values should be determined and transferred to selected cytometers. In this study, we optimized a procedure for instrument standardization across three different flow cytometer platforms from the same vendor and in two different locations. The biomarker quantification was implemented on standardized instruments using CD4 expression on T lymphocytes with a known amount of antibody bound per cell as a quantification standard. Our results on blood cell subset typing and CD19 quantification demonstrated that consistent and reliable results could be accomplished between instruments using the developed procedure. Quantitating the expression levels of certain cell biomarkers relative to a known reference marker before, during, and after therapy would provide important information for monitoring antibody-based therapy and could be potentially used to adjust dosing. Presently, we are implementing this protocol to quantify critical disease biomarkers, and making necessary modifications to the procedure to include instruments from different instrument manufacturers.展开更多
In human life,water resources are inseparable.In recent years,China’s population growth has accelerated,and the industrial level has been continuously improved,resulting in the rapid use and waste of water resources....In human life,water resources are inseparable.In recent years,China’s population growth has accelerated,and the industrial level has been continuously improved,resulting in the rapid use and waste of water resources.The protection and rational distribution of water resources are the most pressing issue in China’s water resources.At present,the water measuring facilities of the U-shaped channel mainly include a straight wall measuring pool,a parabolic throat measuring pool,and a long throat measuring pool.In view of the problems in irrigation measurement,the empirical water measurement of basic open-channel and automatic flow measurement system in irrigation district is summarized to improve the accuracy,fairness,and rationality of water measurement,and promote irrigation district management to a higher level.展开更多
In Saint-Louis, Senegal, a constructed wetland with horizontal flow reed beds (FHa and FHb) has demonstrated significant efficacy in treating municipal wastewater. Analyzing various treatment stages, the system showed...In Saint-Louis, Senegal, a constructed wetland with horizontal flow reed beds (FHa and FHb) has demonstrated significant efficacy in treating municipal wastewater. Analyzing various treatment stages, the system showed only a slight temperature variation, from an influent average of 26.3°C to an effluent of 24.7°C. Electrical conductivity decreased from 1331 mS/cm to 974.5 mS/cm post-primary treatment, with suspended solids (SS) dramatically reduced from 718.9 mg/L to 5.7 mg/L in the final effluent. Biochemical oxygen demand (BOD5) and chemical oxygen demand (COD) saw a notable decrease, from initial levels of 655.6 mg/L and 1240 mg/L to 2.3 mg/L and 71.3 mg/L, respectively. Nitrogenous compounds (N-TN) and phosphates () also decreased significantly, indicating the system’s nutrient removal capacity. Microbiological analysis revealed a reduction in fecal coliforms from 7.5 Ulog/100ml to 1.8 Ulog/100ml and a complete elimination of helminth eggs. The presence of Phragmites and Typha was instrumental in enhancing these reductions. The system’s compliance with the Senegalese standards for disposal into natural environments, WHO recommendations for unrestricted water reuse in irrigation, and the European legislation for water reuse was established. The effluent quality met the stringent criteria for various classes of agricultural reuse, illustrating the system’s potential for sustainable water management. This wetland model presents a robust solution for water-stressed regions, ensuring environmental protection while supporting agricultural needs. The study calls for ongoing research to further refine the system for optimal, reliable wastewater treatment and water resource sustainability.展开更多
Based on the author's practice in river harnessing, this paper defines that a healthy river is a river whose social and natural functions can be balanced or compromised in terms of the socio-economic, ecological and ...Based on the author's practice in river harnessing, this paper defines that a healthy river is a river whose social and natural functions can be balanced or compromised in terms of the socio-economic, ecological and environmental values associated with the river. The environmental values of river systems should be judged according to the following criteria: the signal of a healthy river should be associated with favorable riverbed, acceptable water quality, sustainable river ecosystem and compatible runoff. The river health criterion should reflect the river's natural function status which includes the riverbed, water quality, river ecosystem and runoff. But, the variety and quantity would be different for different rivers depending on different natural features and social background. The standards to be adopted for a healthy river should be determined according to the requirements for maintaining river's normal natural functions and the extent whether the social and natural functions could perform in a balanced way, and also the standards adopted should be adjusted according to the change of the given conditions. The key factor of river health is the enough and clean flow. The authors stressed that human activities would hurt the river health which include excessive water di- version and excessive power generation from the river, improper regulation of flood and sediment, and over discharge of sewage into the river and over change in fish habitat. Taking the Yellow River as a case, this paper also discussed the method to identify what are the standards of a healthy river as well as environmental flows.展开更多
The experimental investigation of unsteady complex flow fields in wind tunnels requires advanced measurement techniques. The most important of such image based measurement techniques are those for the measurement of p...The experimental investigation of unsteady complex flow fields in wind tunnels requires advanced measurement techniques. The most important of such image based measurement techniques are those for the measurement of planar flow velocity fields, planar pressure distribution, model location and deformation, model temperature and quantitative high speed flow visualization. The applications as carried out by DLR range from low speed flows to transonic flows, from high lift configurations to propellers and rotors, from wake vortex investigations in catapult facilities and water towing tanks to investigations of vortex break down phenomena on delta wings. The capability to use image based measurement techniques in transonic flows requires dedicated technical developments and experienced scientists due to the special environment of a transonic wind tunnel. In this paper an overview of the state-of-the art of the application of image based measurement techniques in transonic flows as performed by DLR's Institute of Aerodynamics and Flow Technology will be given.展开更多
Immunophenotyping is proving crucial to understanding the role of the immune system in health and disease.High-through-put flow cytometry has been used extensively to reveal changes in immune cell composition and func...Immunophenotyping is proving crucial to understanding the role of the immune system in health and disease.High-through-put flow cytometry has been used extensively to reveal changes in immune cell composition and function at the single-cell level.Here,we describe six optimized 11-color flow cytometry panels for deep immunophenotyping of human whole blood.A total of 51 surface antibodies,which are readily available and validated,were selected to identify the key immune cell populations and evaluate their functional state in a single assay.The gating strategies for effective flow cytometry data analysis are included in the protocol.To ensure data reproducibility,we provide detailed procedures in three parts,including(1)instrument characterization and detector gain optimization,(2)antibody titration and sample staining,and(3)data acquisition and quality checks.This standardized approach has been applied to a variety of donors for a better understanding of the complexity of the human immune system.展开更多
商业油流是储量起算标准的重要经济评价指标之一。中国石油行业标准Q/ SY 01180—2020《石油天然气经济可采储量评价规范》中给出的常规油藏稳定递减单井商业油流的计算方法,对生产初期产量递减较快、中后期产量递减相对平缓的“分段式...商业油流是储量起算标准的重要经济评价指标之一。中国石油行业标准Q/ SY 01180—2020《石油天然气经济可采储量评价规范》中给出的常规油藏稳定递减单井商业油流的计算方法,对生产初期产量递减较快、中后期产量递减相对平缓的“分段式”递减的特低渗油藏适用性较差。为此,基于常规油藏单一递减率的单井商业油流计算方法,应用盈亏平衡原理,结合“分段式”递减规律,建立了一种符合“分段式”递减规律的油藏单井商业油流计算模型,并以辽河坳陷太阳组为例,结合其油藏相关物性参数、单井投资及成本费用、油气价格等参数,利用所建立的“分段式”递减油藏单井商业油流计算模型,确定了评价油价为60美元/桶条件下的单井商业油流。研究结果对同类型油藏具有借鉴意义。展开更多
文摘With the implementation of the Official Mexican Standard NOM-011-CONAGUA-2000 [1], the water balance of 730 basins has been calculated and its water availability agreement is published. This rule points out to allocate water for the environment only as an annual volume since methods for estimating environmental flows were not standardized in the country. For this reason, The Water Agency (CONAGUA) issued the standard NMX-AA-159-SCFI-2012 [2], to assess environmental flows needed both, at the strategic level in Integrated Water Resources Management (IWRM), or as part of the Environmental Impact Assessment (EIA) of large hydraulic projects. For over ten years, this standard was developed and finally published in September 2012 [3]. It explains different methods from hydrological to holistic approaches, with examples for the country. Its application will cover the urgent need to preserve water for ecosystems in watersheds with high ecological importance and low stress for water use. In this paper, an analysis of the environmental flow standard and examples of the suggested hydrological methods are presented. For its implementation, some steps are taking place, mainly establishing environmental water reserves and building capacities. In addition, environmental allocations are becoming a common practice for all water projects, as well as setting limits to hydrological alterations by hydroelectric dams. The standard promotes the use of technical integration tools to analyze the responses of ecosystems to changes in the flow regime and adaptive management under different scenarios of water use. Although the main steps have been taken, its implementation as mandatory rule will take time.
文摘There is an urgent need for developing a procedure for biomarker standardization and relative quantificationin clinical laboratories. Measuring the expression levels of cell antigens is critical for the diagnosis of many diseases, e.g. leukemia, lymphoma and immunodeficiency diseases. One of the most significant challenges in flow cytometry is obtaining inter-laboratory and intra-laboratory consistent and reproducible results across multiple cytometer platforms and locations longitudinally over time. To obtain measurement consistency, the target flow cytometer voltages should be optimized to segregate the negative population from the electronic noise, and to keep the brightest positive population within the dynamic range of each detector. Then target values should be determined and transferred to selected cytometers. In this study, we optimized a procedure for instrument standardization across three different flow cytometer platforms from the same vendor and in two different locations. The biomarker quantification was implemented on standardized instruments using CD4 expression on T lymphocytes with a known amount of antibody bound per cell as a quantification standard. Our results on blood cell subset typing and CD19 quantification demonstrated that consistent and reliable results could be accomplished between instruments using the developed procedure. Quantitating the expression levels of certain cell biomarkers relative to a known reference marker before, during, and after therapy would provide important information for monitoring antibody-based therapy and could be potentially used to adjust dosing. Presently, we are implementing this protocol to quantify critical disease biomarkers, and making necessary modifications to the procedure to include instruments from different instrument manufacturers.
文摘In human life,water resources are inseparable.In recent years,China’s population growth has accelerated,and the industrial level has been continuously improved,resulting in the rapid use and waste of water resources.The protection and rational distribution of water resources are the most pressing issue in China’s water resources.At present,the water measuring facilities of the U-shaped channel mainly include a straight wall measuring pool,a parabolic throat measuring pool,and a long throat measuring pool.In view of the problems in irrigation measurement,the empirical water measurement of basic open-channel and automatic flow measurement system in irrigation district is summarized to improve the accuracy,fairness,and rationality of water measurement,and promote irrigation district management to a higher level.
文摘In Saint-Louis, Senegal, a constructed wetland with horizontal flow reed beds (FHa and FHb) has demonstrated significant efficacy in treating municipal wastewater. Analyzing various treatment stages, the system showed only a slight temperature variation, from an influent average of 26.3°C to an effluent of 24.7°C. Electrical conductivity decreased from 1331 mS/cm to 974.5 mS/cm post-primary treatment, with suspended solids (SS) dramatically reduced from 718.9 mg/L to 5.7 mg/L in the final effluent. Biochemical oxygen demand (BOD5) and chemical oxygen demand (COD) saw a notable decrease, from initial levels of 655.6 mg/L and 1240 mg/L to 2.3 mg/L and 71.3 mg/L, respectively. Nitrogenous compounds (N-TN) and phosphates () also decreased significantly, indicating the system’s nutrient removal capacity. Microbiological analysis revealed a reduction in fecal coliforms from 7.5 Ulog/100ml to 1.8 Ulog/100ml and a complete elimination of helminth eggs. The presence of Phragmites and Typha was instrumental in enhancing these reductions. The system’s compliance with the Senegalese standards for disposal into natural environments, WHO recommendations for unrestricted water reuse in irrigation, and the European legislation for water reuse was established. The effluent quality met the stringent criteria for various classes of agricultural reuse, illustrating the system’s potential for sustainable water management. This wetland model presents a robust solution for water-stressed regions, ensuring environmental protection while supporting agricultural needs. The study calls for ongoing research to further refine the system for optimal, reliable wastewater treatment and water resource sustainability.
基金Chinese National Research Program,No.2006BAB06BNational Key Basic Research Development Program of China "973",No.G1999043601
文摘Based on the author's practice in river harnessing, this paper defines that a healthy river is a river whose social and natural functions can be balanced or compromised in terms of the socio-economic, ecological and environmental values associated with the river. The environmental values of river systems should be judged according to the following criteria: the signal of a healthy river should be associated with favorable riverbed, acceptable water quality, sustainable river ecosystem and compatible runoff. The river health criterion should reflect the river's natural function status which includes the riverbed, water quality, river ecosystem and runoff. But, the variety and quantity would be different for different rivers depending on different natural features and social background. The standards to be adopted for a healthy river should be determined according to the requirements for maintaining river's normal natural functions and the extent whether the social and natural functions could perform in a balanced way, and also the standards adopted should be adjusted according to the change of the given conditions. The key factor of river health is the enough and clean flow. The authors stressed that human activities would hurt the river health which include excessive water di- version and excessive power generation from the river, improper regulation of flood and sediment, and over discharge of sewage into the river and over change in fish habitat. Taking the Yellow River as a case, this paper also discussed the method to identify what are the standards of a healthy river as well as environmental flows.
文摘The experimental investigation of unsteady complex flow fields in wind tunnels requires advanced measurement techniques. The most important of such image based measurement techniques are those for the measurement of planar flow velocity fields, planar pressure distribution, model location and deformation, model temperature and quantitative high speed flow visualization. The applications as carried out by DLR range from low speed flows to transonic flows, from high lift configurations to propellers and rotors, from wake vortex investigations in catapult facilities and water towing tanks to investigations of vortex break down phenomena on delta wings. The capability to use image based measurement techniques in transonic flows requires dedicated technical developments and experienced scientists due to the special environment of a transonic wind tunnel. In this paper an overview of the state-of-the art of the application of image based measurement techniques in transonic flows as performed by DLR's Institute of Aerodynamics and Flow Technology will be given.
基金supported by the National Key Research and Development Program of China(2021YFA1301000)Shanghai Municipal Science and Technology Major Project(Grant No.2017SHZDZX01).
文摘Immunophenotyping is proving crucial to understanding the role of the immune system in health and disease.High-through-put flow cytometry has been used extensively to reveal changes in immune cell composition and function at the single-cell level.Here,we describe six optimized 11-color flow cytometry panels for deep immunophenotyping of human whole blood.A total of 51 surface antibodies,which are readily available and validated,were selected to identify the key immune cell populations and evaluate their functional state in a single assay.The gating strategies for effective flow cytometry data analysis are included in the protocol.To ensure data reproducibility,we provide detailed procedures in three parts,including(1)instrument characterization and detector gain optimization,(2)antibody titration and sample staining,and(3)data acquisition and quality checks.This standardized approach has been applied to a variety of donors for a better understanding of the complexity of the human immune system.
文摘商业油流是储量起算标准的重要经济评价指标之一。中国石油行业标准Q/ SY 01180—2020《石油天然气经济可采储量评价规范》中给出的常规油藏稳定递减单井商业油流的计算方法,对生产初期产量递减较快、中后期产量递减相对平缓的“分段式”递减的特低渗油藏适用性较差。为此,基于常规油藏单一递减率的单井商业油流计算方法,应用盈亏平衡原理,结合“分段式”递减规律,建立了一种符合“分段式”递减规律的油藏单井商业油流计算模型,并以辽河坳陷太阳组为例,结合其油藏相关物性参数、单井投资及成本费用、油气价格等参数,利用所建立的“分段式”递减油藏单井商业油流计算模型,确定了评价油价为60美元/桶条件下的单井商业油流。研究结果对同类型油藏具有借鉴意义。