Purified human LDH-C4was employed to immunize BALB/c mice. After fusion of the spleen cells from these immunized mice with myeloma, the hybridoma cells were
The paper reported the results of localization of LDH-C4 on sperm of various species by immunqftuorescent test. McAbs to mouse LDH-C4 which reacted not only with mouse sperm, but also with that of other species such a...The paper reported the results of localization of LDH-C4 on sperm of various species by immunqftuorescent test. McAbs to mouse LDH-C4 which reacted not only with mouse sperm, but also with that of other species such as rat, hamster, rabbit and human were used in this study. It is clear that LDH-C4 lacks species specificity. However, there exist different regions for localization of LDH-C4 on sperm of various species, It is suggested that gametes in various species may also differ in their LDH-C4 surface expression. The present study also showed that these McAbs to mouse LDH-C4 have different binding capacity to sperm qf varlous species. The result that LDH-C4 was mainly localized on midpiece of mouse sperm in our experiments is in discrepancy with those previously reported by other investigators.展开更多
The lactate dehydrogenase isoenzyme C4 ofrat testicle was purified with a yield of 1 2.3% anda raise of specific activity to 784-fold.The purified product was PAGE homogeneous and migrated afterLDH3 during electrophor...The lactate dehydrogenase isoenzyme C4 ofrat testicle was purified with a yield of 1 2.3% anda raise of specific activity to 784-fold.The purified product was PAGE homogeneous and migrated afterLDH3 during electrophoresis.展开更多
文摘Purified human LDH-C4was employed to immunize BALB/c mice. After fusion of the spleen cells from these immunized mice with myeloma, the hybridoma cells were
文摘The paper reported the results of localization of LDH-C4 on sperm of various species by immunqftuorescent test. McAbs to mouse LDH-C4 which reacted not only with mouse sperm, but also with that of other species such as rat, hamster, rabbit and human were used in this study. It is clear that LDH-C4 lacks species specificity. However, there exist different regions for localization of LDH-C4 on sperm of various species, It is suggested that gametes in various species may also differ in their LDH-C4 surface expression. The present study also showed that these McAbs to mouse LDH-C4 have different binding capacity to sperm qf varlous species. The result that LDH-C4 was mainly localized on midpiece of mouse sperm in our experiments is in discrepancy with those previously reported by other investigators.
文摘The lactate dehydrogenase isoenzyme C4 ofrat testicle was purified with a yield of 1 2.3% anda raise of specific activity to 784-fold.The purified product was PAGE homogeneous and migrated afterLDH3 during electrophoresis.
