We successfully identified a novel and unique OsbZIP transcription factor,OsbZIP09,whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type,but shared similar germination rate as t...We successfully identified a novel and unique OsbZIP transcription factor,OsbZIP09,whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type,but shared similar germination rate as the wild type under normal germination conditions.The expression of OsbZIP09 was induced by abscisic acid(ABA)and declined as the germination process.As a nucleus-localized transcription factor,the conserved binding motif of OsbZIP09 was identified via DNA affinity purification sequencing technique.Further evidences indicated that OsbZIP09 directly enhanced the expression of ABA catabolism gene ABA8ox1,thus reducing ABA accumulation.In addition,OsbZIP09 also directly bound to the promoter of LEA3 gene to inhibit its expression,thus further alleviating the suppressive effect of ABA on seed germination.These results demonstrated that OsbZIP09 likely functions as a brake of the ABA pathway to attenuate the inhibitory effect of ABA on rice seed germination via dual strategies.展开更多
基金supported by the National Training Programs of Innovation and Entrepreneurship for Undergraduates,Science Fund for Distinguished Young Scholars of Jiangsu Province,China(Grant No.BK20200045)the Priority Academic Program Development of Jiangsu Higher Education Institutions Program,China.
文摘We successfully identified a novel and unique OsbZIP transcription factor,OsbZIP09,whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type,but shared similar germination rate as the wild type under normal germination conditions.The expression of OsbZIP09 was induced by abscisic acid(ABA)and declined as the germination process.As a nucleus-localized transcription factor,the conserved binding motif of OsbZIP09 was identified via DNA affinity purification sequencing technique.Further evidences indicated that OsbZIP09 directly enhanced the expression of ABA catabolism gene ABA8ox1,thus reducing ABA accumulation.In addition,OsbZIP09 also directly bound to the promoter of LEA3 gene to inhibit its expression,thus further alleviating the suppressive effect of ABA on seed germination.These results demonstrated that OsbZIP09 likely functions as a brake of the ABA pathway to attenuate the inhibitory effect of ABA on rice seed germination via dual strategies.
基金国家自然科学基金资助项目(31460202)云南省中青年学术和技术带头人培养项目(2015HB046,2018-H B 024)+2 种基金云南省高校科技创新团队支持计划资助国家林业局西南风景园林工程技术研究中心项目(2016-48)云南省园林植物与观赏园艺省级重点学科和云南省高校园林植物与观赏园艺重点实验室资助项目(50097401)共同资助。
