Effects of different LEDs light spectrum on the growth,leaf anatomy,and chloroplast ultrastructure of potato plantlets in vitro and minituber production after transplanting in the greenhouse

Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m^-2s^-1 total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable;BB and RB could be used as alternatives.

Transcriptome analysis reveals effects of red and blue lightemitting diodes(LEDs)on the growth,chlorophyll fluorescence and endogenous plant hormones of potato(Solanum tuberosum L.)plantlets cultured in vitro

Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.

Effects of stimulating frequency of NIR LEDs light irradiation on forehead as quantified by EEG measurements

Near-infrared(NIR)light has been shown to produce a range of physiological effects in hunans,however,there is still no agreement on whether and how a single parameter,like the flicker frequency of NIR light,affects the brain.An 810 nm NIR LED was used as the stimulator.Fifty subjects participated in this experiment.Forty subjects were randomly divided into four groups.Each group underwent a 30-minute NIR LED radiation with four different frequencies(i.e.,0 Hz,5 Hz,10 Hz and 20 Hz,respectively)on the forehead.The remaining 10 subjects formed the control group,in which they underwent a 30-minute rest period without light radiation.EEG signals of all subjects during each test were recorded.Gravity frequency(GF),relative energy change,and sample entropy were analyzed.The experimental groups had larger GF values compared to the control group.Higher stimulation frequency would cause larger growth of GF(F=14.75,P<0.001).The amplitude of alpha waves relative energy increased,while theta waves decreased remarkably in the experimental groups(p<0.02),and the extent of increase/decrease was larger at higher stimulation frequency,compared to that of the control.Sample entropy of electrodes in the frontal areas were much larger than those in other brain areas in the experimental groups(p<0.001).Larger frequency of the NIR LED light would cause more distinct brain activities in the stimulated areas.It indicates that NIR LED light may have a positive effect on modulating brain activity.These results may help improve the design of photobiomodulation treatments in the future.

Optical wireless network in home area based on white LED lights

A new optical wireless network based on white LED lights in home area is proposed. It is a duplex channel communication system utilizing signal emitters of white LED lights in the downlink and infrared devices in the uplink. Strategies for the system construction are given. Performance for users＇ mobile access of different kinds of optical concentrators is investigated. Indoor channel band-width is analyzed considering white LED light distribution, wall reflection, and receivers field of view （FOV）. Various modulation methods are discussed. A demo point-to-point system for surfing the net is constructed using a new line code of 4B6B which is developed to stabilize the optical output, and a peak data rate of 1 Mbps is achieved. This system is built into the lighting infrastructure, therefore decreasing the cost and offering a solution for wireless access in places which are sensitive to radio frequency like hospitals.

Standards for Tunnel and Road LED Lighting Application Technologies

The paper summarizes the research on the development of standards for tunnels and roads LED lighiing application in Shanghai. analyzes the existing problems on LED road lamps and lighting design, introduces the experiments and testing results, and summarizes current problems of LED street lighting, proposing that LED lighting energy conservation should he ＂systematic energy saving＂ throughout the full life cycle based on the integration of products and applications.

New High Efficiency LED Lighting Solution

As the quality of power LED improves and the cost of power LED reduces, semiconductor lighting will replace incandescent and fluorescent lighting gradually, causing another revolution on the lighting history. And its driving solution has been greatly accelerated. Based on the white power LED I-V characteristics and the application ambiance, proposed is a new LED lighting solution, suiting indoor and outdoor illumination. According to the test results, the design is optimized, and the electrical efficiency is 95% and the output current deviation is 13.0%.

Effects of Low Temperature and Poor Light Environments under LED Lighting on Quality Change of Pepper Seedlings during Storage

Storage and transportation of vegetable commercial seedlings becomes an important link along with the continuous expansion of planting areas of facility vegetables and vegetable seedling culture industry. In this study,pepper variety‘Nongda 24' was as the experimental material,and changes in the morphological and physiological indicators of plug seedlings in different low temperature( 6 and 11℃) and poor light environments were studied to find suitable storage environmental conditions. The results showed that pepper seedlings could be stored and transported for 14 days when storage temperature was 11℃ and light conditions such as light intensity and photoperiod were 30 μmol/( m^2·s)and 12 h/d by using a fluorescent lamp with R∶ B ratio of 1. 9,and 15 μmol/( m^2·s) and 24 h/d by using LED with R∶ B ratio of 1. 3,respectively. Moreover,the quality of pepper seedlings could be protected effectively. Compared to the fluorescent lamp,LED with high luminous efficiency and low energy consumption was suitable for the storage or long-distance transportation of pepper seedlings in low temperature and poor light environments.

Treatment of Peripheral Neuropathy: Combination Therapy Using LED Light, Extracorporeal Shockwave Therapy, Platelet Rich Plasma, and an Oral Dietary Supplement

Objectives: Peripheral neuropathy (PN) is a significant contributor to disability in the elderly. It is also one of the most prevalent complications of type 2 diabetes, prediabetes and metabolic syndrome. PN is commonly associated with pain, numbness, tingling, burning, and cramping in the feet and legs. Current treatment options are limited to controlling pain, seizures and use of antidepressant medications. These treatments have undesirable side effects and don’t stop PN progression. Here we utilized a combination of individual-specific modalities to improve local circulation and relieve PN symptoms. Methods: We conducted an open-label, multicenter pilot trial with 34 subjects (19 males and 15 females ranging from 40 - 85 years of age). All of the participants were diagnosed with peripheral neuropathy and had bilateral symptoms in their feet, and many reported the same symptoms (pain, numbness, tingling, burning, and cramping) in their lower legs. The duration of symptoms ranged from four months to over six years. On Day 0, subjects were given a 90-day supply of the oral supplement with dosing instructions and a LED light therapy device. They also received three platelet-rich plasma (PRP) injections in their lower extremities. Subjects also received an extracorporeal shockwave therapy (ESWT) treatment for each foot and subsequently twice per week for the first six weeks, then once weekly for the duration of the study. Subjects filled out the Brief Pain Index (BPI) at weekly intervals. On Day 90, subjects completed the Patient Global Impression of Change (PGIC) survey. Results: There were significant responses to pain, as evidenced by BPI scores at weeks 8, 9, 10 and 11 (p = 0.02, 0.01, 0.02, and 0.003, respectively). Analysis of the final day PGIC survey showed a favorable outcome for 73% of participants (p = 0.003), with the majority reporting Very Much Improved. Conclusions: By utilizing a multi-modality treatment protocol that includes PRP, LED light therapy, ESWT and an oral dietary supplement, we observed significant reductions in BPI scores. Quality of life and their overall impression of change (PGIC) were significantly improved, and there were no significant side effects.

New LED light source system with uniform illumination for line-scan CCD imaging systems

A system with uniform light reflection in the inner surface within a horizontal 2/3 cylindrical structure for line-scan CCD of the print testing was designed. The design was based on diffuse reflection uniformity of the integrating sphere and requirement of the strip uniform illumination region. This system was called dome light. White light LED array light sources were used for uniform illumi- nation. The LEDs were filtrated to composite array light source based on coefficient of variation of a single LED. The standard white board and SG color checkers were used in the line-scan CCD imaging experiments under the dome light and ordinary illumination light source. The average color difference （AE） of SG color checkers in CIELAB space was 2. 091 under the dome light and 2. 286 under ordinary illumination light source respectively. Experimental results indicate that the dome light can satisfy illumination uniformity and color rendering consistency for line-scan CCD and provide a standard light source for uniform calibration of different cameras.

Controlled LED Lighting for Horticulture: A Review

As energy gradually becomes a more valuable commodity, the desire for reduced energy losses strengthens. Lighting is a critical field on this matter, as it accounts for a large percentage of the global electricity consumption and modern lighting systems are greatly more efficient than incandescent, discharge, and fluorescent lights. Previous research has proven that plants do not require the entire visible spectrum but react only to specific wavelengths, making it possible to control their growth and yield via artificial lighting. The flexibility of control of Light Emitting Diode (LED) lights allows for the combination of great energy losses reduction and controlled plant growth, achieving the improvement of two major parameters in a single action. This review paper summarizes the current research on the effect different light wavelengths have on specific plant species and discusses the applications of LED lighting for horticulture, yield storage, and disease protection.

Accelerated Luminous Depreciation Testing for LED Lighting Products

With the application of energy-saving, environmental and durable LED lighting products, the reliability testing has become a pivot point for the large scale industrialization and popularization. The paper briefly introduces two methods to be respectively applied in the accelerated depreciatinn lesting for the luminous flex of LED lighting products and the life of outdoor LED-driving power, which can sharply shorten the time for lifetime lest. providing references for the LED lighting products＇ reliability evaluation.

测评一款LED灯具——GLP Impression X4 Bar 20

通过对Impression X4 Bar 20的测试,解析该LED灯具的构成、功能及其性能和特点。

Analysis of Current Research and Future Development Trends of Applying Solar Energy in Street Lighting

In the context of promoting green energy transition and addressing climate change globally,solar energy,as a clean and renewable energy source,has gradually become a hot topic for research.Solar streetlight systems realize energy self-sufficiency and environment-friendly lighting by integrating photovoltaic power generation technology and efficient LED lighting technology.By comprehensively analyzing the current status of the application of solar streetlights at home and abroad,this paper discusses its technical advantages,market penetration,and challenges in its development.In terms of technical characteristics,this paper focuses on analyzing the key technologies such as energy conversion efficiency and intelligent control systems of solar streetlights.

建筑结构课程中应用LED灯带制作简支梁配筋模型的实践

简支梁配筋模型制作以提高学生的计算能力、识图能力、构建制作安装能力为主要目的,分为外观设计、配筋设计、板材钢筋灯带下料计算、模型粘接绑扎4个步骤来完成。模型制作极大地调动了学生的动手能力,让理论知识更好地应用于实践,凸显了LED灯带的听觉感和视觉感,其生动的模型制作过程让学生学习建筑结构课程更具趣味性,从而达到课程教学目标的会计算、懂识图、能构建安装等要求。

LED在中大尺寸液晶显示器中的应用

由于LED不含汞,耗电少,效率高和寿命长的特性,2008年在中大尺寸液晶显示器中的应用呈现飞速发展的态势。本文分别阐述了LED在笔记型(Note Book)、桌上型(Desk-top)和液晶电视(LCD-TV)中的应用和需要解决的技术问题并提出了相应的解决方案。

笔记本电脑用LED背光组件的设计开发

目前,小尺寸LED背光源具有极高的市场占有率。文章介绍了笔记本电脑用LED背光源的设计开发,分别从光学、电路、结构角度阐述了开发的过程。光学方面,采用STAMPER技术,一体成型射出导光板,减少印刷环节,无印刷污染;电路方面,根据LED的规格及颗数设计LED发光条,驱动整个背光源;结构方面,采用无背板的结构设计,达到超薄效果。文中设计的笔记本用LED背光源超薄、高亮、低功耗,达业界领先水平。

Light-emitting-diode induced retinal damage and its wavelength dependency in vivo

AIM： To examine light-emitting-diode（LED）-induced retinal neuronal cell damage and its wavelength-driven pathogenic mechanisms.METHODS： Sprague-Dawley rats were exposed to blue LEDs（460 nm）,green LEDs（530 nm）,and red LEDs（620 nm）.Electroretinography（ERG）,Hematoxylin and eosin（H＆E） staining,transmission electron microscopy（TEM）,terminal deoxynucleotidyl transferase d UTP nick end labeling（TUNEL）,and immunohistochemical（IHC） staining,Western blotting（WB） and the detection of superoxide anion（O2^-·）,hydrogen peroxide（H2O2）,total iron,and ferric（Fe^3＋） levels were applied.RESULTS： ERG results showed the blue LED group induced more functional damage than that of green or red LED groups.H＆E staining,TUNEL,IHC,and TEM revealed apoptosis and necrosis of photoreceptors and RPE,which indicated blue LED also induced more photochemical injury.Free radical production and iron-related molecular marker expressions demonstrated that oxidative stress and ironoverload were associated with retinal injury.WB assays correspondingly showed that defense gene expression was up-regulated after the LED light exposure with a wavelength dependency.CONCLUSION： The study results indicate that LED bluelight exposure poses a great risk of retinal injury in awake,task-oriented rod-dominant animals.The wavelengthdependent effect should be considered carefully when switching to LED lighting applications.

Metabolomic and transcriptomic analysis reveals the molecular mechanism by which blue light promotes lutein synthesis in strawberry

Carotenoids are an important component of the human diet, and fruit is a primary source of carotenoids. The synthesis and regulation of carotenoids in fruit are important contributors to the formation of fruit quality. In China, strawberry is one of the main seasonal fruits grown in the winter. Previous studies have shown that light has a significant effect on the metabolism of anthocyanins, sugars, and polyphenols in strawberry. However, the understanding of the role of light in regulating the metabolism of carotenoids in strawberry remains limited. This study investigated the effects of blue, red, yellow-green, and white light on carotenoid metabolism in strawberry.Blue light treatment promoted the synthesis of multiple carotenoids, including lutein, compared with the other three treatment groups. The RNA sequencing data revealed that blue light treatment promoted the expression of lycopene ε-cyclase(LCYE), and the transient overexpression of LCYE in strawberry fruit promoted lutein accumulation in strawberry. Overall, the results suggest that blue light can promote the synthesis of lutein in strawberry by inducing the expression of LCYE.

Blue light induces leaf color change by modulating carotenoid metabolites in orange-head Chinese cabbage(Brassica rapa L.ssp. pekinensis)

Carotenoids are involved in the formation of plant leaf color as well as photosystem photoprotection. This study showed that blue light significantly induced up-regulation of the total carotenoid content in the inner leaves of orange-head Chinese cabbage(OHCC). Furthermore, the transcriptomic analysis revealed that blue light treatment induced upregulation of genes in photosynthesis(BrHY5-2, BrCOP1 and BrDET1) and the methylerythritol 4-phosphate pathways(BrGGPS, BrDXS and BrHDR) upstream of the carotenoid metabolic pathway. Carotenoid metabolomic analysis revealed that the accumulation of several orange and red carotenoids(lycopene, zeaxanthin, β-carotene, lutein, and β-cryptoxanthin) after blue light treatment contributed to the deepening of the leaf coloration, suggesting that short-term blue light treatment could be used to boost nutritional quality. The light signal gene BrHY5-2 participated in the blue light-induced transcriptional regulation of carotenoid biosynthesis in OHCC. Overexpression of BrHY5-2 in Arabidopsis significantly increased the total carotenoid content and the sensitivity to blue light. The above findings revealed new insights about blue-light-induced carotenoid synthesis and accumulation in OHCC lines. They suggested a new engineering approach to increase the nutritional value of vegetables.

Design and Analysis of Traffic Information Transmission System Based on Visible Light Communication

Visible Light Communication( VLC) based on LED is a new wireless communication technology with high response rate and good modulation characteristics in the wavelengths of 380- 780 nm. Compared with conventional methods,the waveband of VLC is harmless to human and safe to communication because of no magnetism radiation. An audio information transmission system using LED traffic lights is presented based on VLC technology. The system is consisted of transmitting terminal,receiving terminal and communication channel. Some experiments were made under real communication environment. The experimental results showed that the traffic information transmission system works steadily with good communication quality and achieves the purpose of transmitting audio information through LED traffic lights,with a data transfer rate up to 250 kbps over a distance of 5 meters.