In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Sc...In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average recovery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene content in edible oil.展开更多
In X-ray absorption fine structure(XAFS) experiments,Soller slits are widely used as filter devices in order to improve the signal to noise ratio.Performing high accuracy manual focusing operations is a time-consuming...In X-ray absorption fine structure(XAFS) experiments,Soller slits are widely used as filter devices in order to improve the signal to noise ratio.Performing high accuracy manual focusing operations is a time-consuming process;therefore,this work introduces an automatic focusing method for Soller slits in multi-element fluorescence detectors.This method establishes a relation model between the fluorescence intensity distribution and the coordinates of the fluorescence excitation point.According to this relation model,the actual coordinates of the fluorescence excitation point can be deduced from the detected fluorescence intensity distribution and used in focusing operations.This method has proven to be feasible in an XAFS experiment at the BL14W1 beamline of the Shanghai Synchrotron Radiation Facility.展开更多
Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitorin...Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.展开更多
JCT-2000 micro-computer-controlled universal fluorescent magnetic flaw detector, made by the Tianjin No.6 Machine Tool Plant, is used to detect flaws and fatigued cracks from forged products,machined products and
A semi-empirical detector response function(DRF)model is established to fit characteristic X-ray peaks recorded in Si-PIN spectra,which is mainly composed of four components:a truncated step function,a Gaussian-shaped...A semi-empirical detector response function(DRF)model is established to fit characteristic X-ray peaks recorded in Si-PIN spectra,which is mainly composed of four components:a truncated step function,a Gaussian-shaped full-energy peak,a Gaussian-shaped Si escape peak and an exponential tail.A simple but useful statistical distribution-based analytic method(SDA)is proposed to achieve accurate values of standard deviation for characteristic X-ray peaks.And the values of the model parameters except for the standard deviation are obtained by weighted least-squares fitting of the pulse-height spectra from a number of pure-element samples.A Monte Carlo model is also established to simulate the X-ray measurement setup.The simulated flux spectrum can be transformed by Si-PIN detector response function to real pulse height spectrum as studied in this work.Finally,the fitting result for a copper alloy sample was compared with experimental spectra,and the validity of the present method was demonstrated.展开更多
A transient thickness distribution measured with a high temporal resolution is elemental for exploring the flow characteristics and mechanism of a liquid film formed by an impinging jet.Therefore,this paper develops a...A transient thickness distribution measured with a high temporal resolution is elemental for exploring the flow characteristics and mechanism of a liquid film formed by an impinging jet.Therefore,this paper develops a high-speed Light-Emitting Diode-Induced Fluorescence(LEDIF)system based on the brightness measured directly above the liquid film.An Ultraviolet(UV)LED lamp is used to provide sufficient and continuous excitation light.Then,a system performance analysis proves that the system can continuously measure the global film thickness at a high acquisition frequency of 5000 Hz when the dye concentration is 200 mg/L.The influence of the irregularity of the excitation intensity,including the spatial non-uniformity,temporal instability,and long-term instability,on the measurement uncertainty is analyzed in detail.The analysis indicates that the system has an acceptable uncertainty of 10%.Compared with theoretical results,experimental results verify that the LEDIF system can accurately measure the global thickness of a liquid film formed by a water jet obliquely impinging onto a plate.An experimental investigation of the radial section of the raised zone demonstrates that the radial section changes from a sewing needle to an oval when the azimuth angle increases from 10°to 90°.Meanwhile,the dynamic contact angle exponentially decreases from 41.4°to 30.1°.A dynamic analysis of surface waves shows that the measured wave velocity decreases from 12 m/s to 1 m/s and the dominant frequency decreases from 1000 Hz to 10 Hz along the flow direction.展开更多
The technique of fluorescence spectroscopy is applied to study thioredoxin reductase (TrxR) in the cells of human brain. Experimental results show that, by the violet light emitting diode (LED, Amax=407 nm) light irra...The technique of fluorescence spectroscopy is applied to study thioredoxin reductase (TrxR) in the cells of human brain. Experimental results show that, by the violet light emitting diode (LED, Amax=407 nm) light irradiation, TrxR is able to emit three striking spectral bands (528 - 582 nm; 588 - 660 nm; 683 - 700 nm). The fluorescence intensity is linear to the concentration of TrxR. The spectrum of denatured TrxR is rather different from that of organized TrxR, which reflects the structure change between denatured TrxR and organized TrxR. Furthermore, physical and biochemical mechanisms of fluorescence production for LED light-induced TrxR spectra and its characteristics are analyzed. This paper may be useful to better understand the structure of TrxR, and to provide new spectroscopic information to improve the resolution for this kind of biology structure.展开更多
Sulfur dioxide and its derivative sulfite widely existed in air,water as the environment pollutant.Sulfite is also commonly used as preservative and additive in fresh fruits,vegetables,wines and pharmaceutical materia...Sulfur dioxide and its derivative sulfite widely existed in air,water as the environment pollutant.Sulfite is also commonly used as preservative and additive in fresh fruits,vegetables,wines and pharmaceutical materials.Due to sulfite is closely related with human diseases,it is very urgent for the sensitive and rapid quantification of sulfite in various samples.In our study,a turn-on near infrared(NIR)fluorescent probe(MDQ)was developed for sulfite detection based on a Michael addition reaction,with high sensitivity(LOD 4.16 nmol/L),selectivity and fast response time(400 s).Using MDQ,a quantify method for sulfite in traditional Chinese medicines(TCMs)was developed with the advantages of high precision,accuracy and convenient operation.Furthermore,according to the photophysical property of MDQ,a portable fluorescence detector is designed to quantify sulfite for TCMs and surface water in Dalian city of China.Therefore,the developed fluorescent probe MDQ and portable fluorescent detector as a rapid inspection instrument were successfully used to real-time monitor the sulfite in various complex samples.展开更多
文摘In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average recovery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene content in edible oil.
基金supported by National Nature Science Foundation of China(No.11175244)
文摘In X-ray absorption fine structure(XAFS) experiments,Soller slits are widely used as filter devices in order to improve the signal to noise ratio.Performing high accuracy manual focusing operations is a time-consuming process;therefore,this work introduces an automatic focusing method for Soller slits in multi-element fluorescence detectors.This method establishes a relation model between the fluorescence intensity distribution and the coordinates of the fluorescence excitation point.According to this relation model,the actual coordinates of the fluorescence excitation point can be deduced from the detected fluorescence intensity distribution and used in focusing operations.This method has proven to be feasible in an XAFS experiment at the BL14W1 beamline of the Shanghai Synchrotron Radiation Facility.
基金support from the National Key R&D Program of China(2017YFA0700500)National Natural Science Foundation of China(61775144/61525503/61620106016/61835009/81727804)+2 种基金(Key)Project of Department of Education of Guangdong Province(2015KGJHZ002/2016KCXTD007)Guangdong Natural Science Foundation(2014A030312008,2017A030310132,2018A030313362)Shenzhen Basic Research Project(JCYJ20170818144012025/JCYJ20170818141701667/JCYJ20170412105003520/JCYJ20150930104948169).
文摘Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.
文摘JCT-2000 micro-computer-controlled universal fluorescent magnetic flaw detector, made by the Tianjin No.6 Machine Tool Plant, is used to detect flaws and fatigued cracks from forged products,machined products and
基金Supported by National Natural Science Foundation of China(Nos.40974065 and 41025015)Scientific and Technological Innovative Team in Sichuan Province(No.2011JTD0013)"863"Program of China(No.2012AA063501)
文摘A semi-empirical detector response function(DRF)model is established to fit characteristic X-ray peaks recorded in Si-PIN spectra,which is mainly composed of four components:a truncated step function,a Gaussian-shaped full-energy peak,a Gaussian-shaped Si escape peak and an exponential tail.A simple but useful statistical distribution-based analytic method(SDA)is proposed to achieve accurate values of standard deviation for characteristic X-ray peaks.And the values of the model parameters except for the standard deviation are obtained by weighted least-squares fitting of the pulse-height spectra from a number of pure-element samples.A Monte Carlo model is also established to simulate the X-ray measurement setup.The simulated flux spectrum can be transformed by Si-PIN detector response function to real pulse height spectrum as studied in this work.Finally,the fitting result for a copper alloy sample was compared with experimental spectra,and the validity of the present method was demonstrated.
基金supported by the Provincial or Ministerial Level Project,China。
文摘A transient thickness distribution measured with a high temporal resolution is elemental for exploring the flow characteristics and mechanism of a liquid film formed by an impinging jet.Therefore,this paper develops a high-speed Light-Emitting Diode-Induced Fluorescence(LEDIF)system based on the brightness measured directly above the liquid film.An Ultraviolet(UV)LED lamp is used to provide sufficient and continuous excitation light.Then,a system performance analysis proves that the system can continuously measure the global film thickness at a high acquisition frequency of 5000 Hz when the dye concentration is 200 mg/L.The influence of the irregularity of the excitation intensity,including the spatial non-uniformity,temporal instability,and long-term instability,on the measurement uncertainty is analyzed in detail.The analysis indicates that the system has an acceptable uncertainty of 10%.Compared with theoretical results,experimental results verify that the LEDIF system can accurately measure the global thickness of a liquid film formed by a water jet obliquely impinging onto a plate.An experimental investigation of the radial section of the raised zone demonstrates that the radial section changes from a sewing needle to an oval when the azimuth angle increases from 10°to 90°.Meanwhile,the dynamic contact angle exponentially decreases from 41.4°to 30.1°.A dynamic analysis of surface waves shows that the measured wave velocity decreases from 12 m/s to 1 m/s and the dominant frequency decreases from 1000 Hz to 10 Hz along the flow direction.
文摘The technique of fluorescence spectroscopy is applied to study thioredoxin reductase (TrxR) in the cells of human brain. Experimental results show that, by the violet light emitting diode (LED, Amax=407 nm) light irradiation, TrxR is able to emit three striking spectral bands (528 - 582 nm; 588 - 660 nm; 683 - 700 nm). The fluorescence intensity is linear to the concentration of TrxR. The spectrum of denatured TrxR is rather different from that of organized TrxR, which reflects the structure change between denatured TrxR and organized TrxR. Furthermore, physical and biochemical mechanisms of fluorescence production for LED light-induced TrxR spectra and its characteristics are analyzed. This paper may be useful to better understand the structure of TrxR, and to provide new spectroscopic information to improve the resolution for this kind of biology structure.
基金supported financially by Distinguished Professor Program of Liaoning Province(No.XLYC2002008)Dalian Science and Technology Leading Talents Project(Nos.2019RD15,2020RJ09 and 2020RQ076)+2 种基金Liaoning Provincial Natural Science Foundation(No.20180550761)Liaoning Revitalization Talents Program(No.XLYC1907017)the Open Research Fund of the School of Chemistry and Chemical Engineering,Henan Normal University(No.2021YB07).
文摘Sulfur dioxide and its derivative sulfite widely existed in air,water as the environment pollutant.Sulfite is also commonly used as preservative and additive in fresh fruits,vegetables,wines and pharmaceutical materials.Due to sulfite is closely related with human diseases,it is very urgent for the sensitive and rapid quantification of sulfite in various samples.In our study,a turn-on near infrared(NIR)fluorescent probe(MDQ)was developed for sulfite detection based on a Michael addition reaction,with high sensitivity(LOD 4.16 nmol/L),selectivity and fast response time(400 s).Using MDQ,a quantify method for sulfite in traditional Chinese medicines(TCMs)was developed with the advantages of high precision,accuracy and convenient operation.Furthermore,according to the photophysical property of MDQ,a portable fluorescence detector is designed to quantify sulfite for TCMs and surface water in Dalian city of China.Therefore,the developed fluorescent probe MDQ and portable fluorescent detector as a rapid inspection instrument were successfully used to real-time monitor the sulfite in various complex samples.
