Pretreatment of lespedeza stalks by steam explosion has been studied. The results indicate that steam-exploded pretreatment has strong effects on physical features, morphology, crystallinity, and composition of lesped...Pretreatment of lespedeza stalks by steam explosion has been studied. The results indicate that steam-exploded pretreatment has strong effects on physical features, morphology, crystallinity, and composition of lespedeza stalks as shown by scanning electron microscopy （SEM）, infrared （IR）, and X-ray diffraction spectrometry methods. After steam explosion, the cellulose and lignin contents of lespedeza stalks varied only slightly, but the hemicellulose content had decreased from 29.34% to 7.48%. The cellulose obtained by steam-exploded pretreatment had a higher degree of crystallinity than that of the raw material. At the explosion condition of 2.25 MPa and 4 min, lignocellulose is easier to hydrolyze by enzyme than the original lignocellulose. The concentration of reduced sugar in the hydrolyzate liquid increased from 71.77 to 162.84 g·L^-1.展开更多
Lespedeza stalks were subjected to steam pretreatment at 210℃ for some steaming time before simultaneous saccharification and fermentation （SSF）. Cellulose-derived glucose was extensively utilized by yeast during S...Lespedeza stalks were subjected to steam pretreatment at 210℃ for some steaming time before simultaneous saccharification and fermentation （SSF）. Cellulose-derived glucose was extensively utilized by yeast during SSE The ethanol yields after steam pretreatment of the lespedeza stalks at 210℃ were 59.3%, 72.8% and 62.2% of the theoretically expected values when the steaming times were 2, 4 and 6 min, respectively. The highest yield from α-cellulose was 92.7% of the theoretical value. Steam explosion pretreatment of lespedeza stalks increased ethanol yields by a factor of 4.4, from 16.4% （untreated） to 72.8% （steam explosion pretreated）.展开更多
Lespedeza bicolor Turcz is a native shrub in the temperate region of China. Thirteen provenances of L. bicolor were investigated in our study. Morphological diversity among and within provenances were analyzed based o...Lespedeza bicolor Turcz is a native shrub in the temperate region of China. Thirteen provenances of L. bicolor were investigated in our study. Morphological diversity among and within provenances were analyzed based on 19 phenotypic traits, including seven vegetative growth characteristics, nine floral traits and three pod characteristics. The coefficient of variation （CV） ranged from 6.4% to 65.4% and the phenotypic differentiation coefficient from 43.3% to 97.3%. Analyses of variance showed that there were extremely significant variations among and within provenances in pod length. Differences in plant height, inter-nodal length, number of branches, leaf width, leaf length and leaf area were not remarkable among provenance, but distinct within provenances. Other traits showed evidence of contrasts. Variation among provenances was the main part of phenotypic variation. Correlation analysis indicated that there were highly significant positive correlations between plant height and other vegetative growth characteristics. Floral traits were not associated with vegetative growth characteristics. The 13 provenances of L. bicolor investigated can be classified into three groups according to a UPGMA cluster analysis.展开更多
Effect of ensiled sericea lespedeza(SL,Lespedeza cuneata)on indicators of gastrointestinal parasitism was investigated in two trials using intact male Spanish goats(n=36/trial).Naturally parasite-infected animals at 2...Effect of ensiled sericea lespedeza(SL,Lespedeza cuneata)on indicators of gastrointestinal parasitism was investigated in two trials using intact male Spanish goats(n=36/trial).Naturally parasite-infected animals at 24.4±3.7 kg body weight(BW)and nine months of age or 24.6±0.57 kg BW at 4-6 months of age were used for 28 d or 21 d for Trials 1 and 2,respectively.For Trial 1,goats were fed SL silage(SLS),SL hay(SLH),or Bermuda grass(BG,Cynodon dactylon)hay at 70%of the diet.For Trial 2,goats were provided with SLH or SLS and orally drenched with distilled water(Hay or silage plus water,HW and SW,respectively)or polyethylene glycol(PEG;SLS only;SP)daily.Fecal and blood samples were collected weekly to determine gastrointestinal nematode fecal egg counts(FEC)and coccidia fecal oocyst counts(FOC)per gram of feces and blood packed cell volume(PCV).The SL diets significantly reduced(p<0.05)FEC and FOC in both trials,with the SLH diet reducing FEC 7 d faster compared to SLS.In PEG-treated goats,FOC tended to increase(p<0.07)at day 7 before decreasing.There was no treatment effect on PCV scores.Overall,SLH and SLS reduced fecal egg and oocyst counts in goats,but PEG results were inconclusive,so more research is needed.展开更多
Two 42-day feeding trials in pens were completed with young goats(Spanish,intact male,six months old,n=29 Trial 1;n=20 Trial 2)to determine the anti-parasitic bioactivity of whole plant and leaf only sericea lespedeza...Two 42-day feeding trials in pens were completed with young goats(Spanish,intact male,six months old,n=29 Trial 1;n=20 Trial 2)to determine the anti-parasitic bioactivity of whole plant and leaf only sericea lespedeza(SL;Lespedeza cuneata)fed as a ground meal or in pelleted form.In Trial 1,goats were fed SL leaf only pellets,SL whole plant pellets,or a commercial goat pellet as 50%of a complete ration,while in Trial 2,kids were fed either ground SL leaf meal or ground whole plant SL meal as 25%of a complete ration.Fecal samples were collected weekly for determination of gastrointestinal nematode(GIN)fecal egg counts(FEC)and coccidia fecal oocyst counts(FOC).In Trial 1,both of the pelleted SL rations reduced FEC(p<0.06)relative to the kids fed the control ration,and for FOC,there were lower values on Days 7 to 42 relative to Day 0 for goats on either pelleted SL diet,while FOC for control animals did not change over time(interaction,p<0.01).In Trial 2,both ground whole plant and leaf only SL diets reduced(p<0.05)FEC of the goats over time,but the effect was faster in the kids on the SL leaf meal ration.At 50%of the diet,SL leaf and whole plant meal pellets were equally effective against GIN egg and coccidial oocyst production in young kids,but SL leaf meal was more effective in reducing GIN egg production than whole plant SL meal when fed at 25%of the diet.展开更多
In this investigation,fresh sericea lespedeza(SL;Lespedeza cuneata)and alfalfa(Medicago sativa)were cut in the field,frozen,chopped and mixed into ratios of 100:0,75:25,50:50,25:75 and 0:100,respectively,with each tre...In this investigation,fresh sericea lespedeza(SL;Lespedeza cuneata)and alfalfa(Medicago sativa)were cut in the field,frozen,chopped and mixed into ratios of 100:0,75:25,50:50,25:75 and 0:100,respectively,with each treatment combination packed into 12 mini-silos and sealed to be air-tight.Three mini-silos per treatment were opened after 1,7,21 and 84 d of ensiling and analyzed for pH,neutral detergent fiber(NDF),acid detergent fiber(ADF),and acid detergent lignin(ADL),unbound and bound condensed tannins(CT),nitrogen(N),nitrate N(NO3-N),and ammonia N(NH4+N)content.All of the forage combinations ensiled well,with a rapid drop in pH(below 5.0 by Day 7).Fiber concentrations(NDF,ADF,ADL)were greater in 75% and 100% SL silages than in 0%,25% and 50% SL samples by Day 84 of the study,possibly due to interference of CT in the detergent analysis system.Concentrations of N,NO3-N and NH4+N were decreased in silages as percentage SL in the mixture increased,while unbound,bound and total CT increased as percentage SL increased.In this study,there was reduced proteolysis during ensiling of combinations of SL and alfalfa,as indicated by reduced NO3-N and NH4+N production as percentage SL in the silage mixtures increased.展开更多
Two new phenylpropanoid glycosides named cuneataside E(1) and cuneataside F(2), were isolated from the aerial parts of Lespedeza cuneata(Dum. Cours.) G. Don, whose structures were E and Z isomer, respectively. Their s...Two new phenylpropanoid glycosides named cuneataside E(1) and cuneataside F(2), were isolated from the aerial parts of Lespedeza cuneata(Dum. Cours.) G. Don, whose structures were E and Z isomer, respectively. Their structures were elucidated on the basis of comprehensive spectroscopic analysis(UV, IR, HR-ESI-MS, 1D and 2D NMR). In in vitro bioassays at 10 μmol/L, compound 1showed moderate hepatoprotective activity against N-acetyl-p-aminophenol(APAP)-induced toxicity in He G2 cells.展开更多
OBJECTIVE: To evaluate the anticancer, antioxidant and antimicrobial activities along with total phenolic and flavonoids contents extracted from Lespedeza bicolor indigenous to Khyber Pukhtoonkhwa Pakistan.METHODS: An...OBJECTIVE: To evaluate the anticancer, antioxidant and antimicrobial activities along with total phenolic and flavonoids contents extracted from Lespedeza bicolor indigenous to Khyber Pukhtoonkhwa Pakistan.METHODS: Anticancer activity against human lung carcinoma(LU-1) and human prostate carcinoma(Ln Cap), antimicrobial against Aspergillus fumigates, Aspergillus niger, Fusarium solani, Mucor sp Klebsiella pneumonia and Enterococcus and antioxidant activity on 1,1-Diphenyl-2-picryl-hydrazy(DPPH) and Hydrogen peroxide radicals scavenging activity were assessed in methanol extract o Lespedeza bicolor.RESULTS: Lespedeza bicolor aerial parts and root extract showed IC_(50)value of 12.5 and 50 μg/m L against LU-1 whereas, ≤ 12.5 and 12 μg/m L were calculated against Ln Cap cell line. Minimum inhibition concentration(MIC) value of 20-35 μg/m L has been observed against Aspergillus fumigates, Aspergillus niger, Fusarium solani and Mucor sp in comparision with 1-2.5 μg/m L of Terbinafine used as a standard fungicide. MIC value of 20 and 35 μg/m L of Lespedeza bicolor aerial parts and root extract against bacterial pathogen Klebsiella pneumonia and 20-50 μg/m L against Enterococcus has been measured. DPPH radical scavenging activity of Lespedeza bicolor with IC_(50)values of ≤ 50 μg/m L and ≤ 200 μg/m L was observed whereas, hydrogen peroxide scavenging activity with IC_(50)values of ≤25 μg/m L for aerial parts and ≤ 50 μg/m L for the root extract of Lespedeza bicolor has been shown.CONCLUSION: These data suggested that the methanolic extract of Lespedeza bicolor could be potential candidates for natural antioxidants and anticancer.展开更多
文摘Pretreatment of lespedeza stalks by steam explosion has been studied. The results indicate that steam-exploded pretreatment has strong effects on physical features, morphology, crystallinity, and composition of lespedeza stalks as shown by scanning electron microscopy （SEM）, infrared （IR）, and X-ray diffraction spectrometry methods. After steam explosion, the cellulose and lignin contents of lespedeza stalks varied only slightly, but the hemicellulose content had decreased from 29.34% to 7.48%. The cellulose obtained by steam-exploded pretreatment had a higher degree of crystallinity than that of the raw material. At the explosion condition of 2.25 MPa and 4 min, lignocellulose is easier to hydrolyze by enzyme than the original lignocellulose. The concentration of reduced sugar in the hydrolyzate liquid increased from 71.77 to 162.84 g·L^-1.
文摘Lespedeza stalks were subjected to steam pretreatment at 210℃ for some steaming time before simultaneous saccharification and fermentation （SSF）. Cellulose-derived glucose was extensively utilized by yeast during SSE The ethanol yields after steam pretreatment of the lespedeza stalks at 210℃ were 59.3%, 72.8% and 62.2% of the theoretically expected values when the steaming times were 2, 4 and 6 min, respectively. The highest yield from α-cellulose was 92.7% of the theoretical value. Steam explosion pretreatment of lespedeza stalks increased ethanol yields by a factor of 4.4, from 16.4% （untreated） to 72.8% （steam explosion pretreated）.
文摘Lespedeza bicolor Turcz is a native shrub in the temperate region of China. Thirteen provenances of L. bicolor were investigated in our study. Morphological diversity among and within provenances were analyzed based on 19 phenotypic traits, including seven vegetative growth characteristics, nine floral traits and three pod characteristics. The coefficient of variation （CV） ranged from 6.4% to 65.4% and the phenotypic differentiation coefficient from 43.3% to 97.3%. Analyses of variance showed that there were extremely significant variations among and within provenances in pod length. Differences in plant height, inter-nodal length, number of branches, leaf width, leaf length and leaf area were not remarkable among provenance, but distinct within provenances. Other traits showed evidence of contrasts. Variation among provenances was the main part of phenotypic variation. Correlation analysis indicated that there were highly significant positive correlations between plant height and other vegetative growth characteristics. Floral traits were not associated with vegetative growth characteristics. The 13 provenances of L. bicolor investigated can be classified into three groups according to a UPGMA cluster analysis.
文摘Effect of ensiled sericea lespedeza(SL,Lespedeza cuneata)on indicators of gastrointestinal parasitism was investigated in two trials using intact male Spanish goats(n=36/trial).Naturally parasite-infected animals at 24.4±3.7 kg body weight(BW)and nine months of age or 24.6±0.57 kg BW at 4-6 months of age were used for 28 d or 21 d for Trials 1 and 2,respectively.For Trial 1,goats were fed SL silage(SLS),SL hay(SLH),or Bermuda grass(BG,Cynodon dactylon)hay at 70%of the diet.For Trial 2,goats were provided with SLH or SLS and orally drenched with distilled water(Hay or silage plus water,HW and SW,respectively)or polyethylene glycol(PEG;SLS only;SP)daily.Fecal and blood samples were collected weekly to determine gastrointestinal nematode fecal egg counts(FEC)and coccidia fecal oocyst counts(FOC)per gram of feces and blood packed cell volume(PCV).The SL diets significantly reduced(p<0.05)FEC and FOC in both trials,with the SLH diet reducing FEC 7 d faster compared to SLS.In PEG-treated goats,FOC tended to increase(p<0.07)at day 7 before decreasing.There was no treatment effect on PCV scores.Overall,SLH and SLS reduced fecal egg and oocyst counts in goats,but PEG results were inconclusive,so more research is needed.
文摘Two 42-day feeding trials in pens were completed with young goats(Spanish,intact male,six months old,n=29 Trial 1;n=20 Trial 2)to determine the anti-parasitic bioactivity of whole plant and leaf only sericea lespedeza(SL;Lespedeza cuneata)fed as a ground meal or in pelleted form.In Trial 1,goats were fed SL leaf only pellets,SL whole plant pellets,or a commercial goat pellet as 50%of a complete ration,while in Trial 2,kids were fed either ground SL leaf meal or ground whole plant SL meal as 25%of a complete ration.Fecal samples were collected weekly for determination of gastrointestinal nematode(GIN)fecal egg counts(FEC)and coccidia fecal oocyst counts(FOC).In Trial 1,both of the pelleted SL rations reduced FEC(p<0.06)relative to the kids fed the control ration,and for FOC,there were lower values on Days 7 to 42 relative to Day 0 for goats on either pelleted SL diet,while FOC for control animals did not change over time(interaction,p<0.01).In Trial 2,both ground whole plant and leaf only SL diets reduced(p<0.05)FEC of the goats over time,but the effect was faster in the kids on the SL leaf meal ration.At 50%of the diet,SL leaf and whole plant meal pellets were equally effective against GIN egg and coccidial oocyst production in young kids,but SL leaf meal was more effective in reducing GIN egg production than whole plant SL meal when fed at 25%of the diet.
文摘In this investigation,fresh sericea lespedeza(SL;Lespedeza cuneata)and alfalfa(Medicago sativa)were cut in the field,frozen,chopped and mixed into ratios of 100:0,75:25,50:50,25:75 and 0:100,respectively,with each treatment combination packed into 12 mini-silos and sealed to be air-tight.Three mini-silos per treatment were opened after 1,7,21 and 84 d of ensiling and analyzed for pH,neutral detergent fiber(NDF),acid detergent fiber(ADF),and acid detergent lignin(ADL),unbound and bound condensed tannins(CT),nitrogen(N),nitrate N(NO3-N),and ammonia N(NH4+N)content.All of the forage combinations ensiled well,with a rapid drop in pH(below 5.0 by Day 7).Fiber concentrations(NDF,ADF,ADL)were greater in 75% and 100% SL silages than in 0%,25% and 50% SL samples by Day 84 of the study,possibly due to interference of CT in the detergent analysis system.Concentrations of N,NO3-N and NH4+N were decreased in silages as percentage SL in the mixture increased,while unbound,bound and total CT increased as percentage SL increased.In this study,there was reduced proteolysis during ensiling of combinations of SL and alfalfa,as indicated by reduced NO3-N and NH4+N production as percentage SL in the silage mixtures increased.
基金financially supported by the National Mega-project for Innovative Drugs(No.2012ZX09301002-002)National Natural Science Foundation of China(Nos.81560632 and 81202546)
文摘Two new phenylpropanoid glycosides named cuneataside E(1) and cuneataside F(2), were isolated from the aerial parts of Lespedeza cuneata(Dum. Cours.) G. Don, whose structures were E and Z isomer, respectively. Their structures were elucidated on the basis of comprehensive spectroscopic analysis(UV, IR, HR-ESI-MS, 1D and 2D NMR). In in vitro bioassays at 10 μmol/L, compound 1showed moderate hepatoprotective activity against N-acetyl-p-aminophenol(APAP)-induced toxicity in He G2 cells.
基金Supported by Higher Education Commission of Pakistan(IRSIP)
文摘OBJECTIVE: To evaluate the anticancer, antioxidant and antimicrobial activities along with total phenolic and flavonoids contents extracted from Lespedeza bicolor indigenous to Khyber Pukhtoonkhwa Pakistan.METHODS: Anticancer activity against human lung carcinoma(LU-1) and human prostate carcinoma(Ln Cap), antimicrobial against Aspergillus fumigates, Aspergillus niger, Fusarium solani, Mucor sp Klebsiella pneumonia and Enterococcus and antioxidant activity on 1,1-Diphenyl-2-picryl-hydrazy(DPPH) and Hydrogen peroxide radicals scavenging activity were assessed in methanol extract o Lespedeza bicolor.RESULTS: Lespedeza bicolor aerial parts and root extract showed IC_(50)value of 12.5 and 50 μg/m L against LU-1 whereas, ≤ 12.5 and 12 μg/m L were calculated against Ln Cap cell line. Minimum inhibition concentration(MIC) value of 20-35 μg/m L has been observed against Aspergillus fumigates, Aspergillus niger, Fusarium solani and Mucor sp in comparision with 1-2.5 μg/m L of Terbinafine used as a standard fungicide. MIC value of 20 and 35 μg/m L of Lespedeza bicolor aerial parts and root extract against bacterial pathogen Klebsiella pneumonia and 20-50 μg/m L against Enterococcus has been measured. DPPH radical scavenging activity of Lespedeza bicolor with IC_(50)values of ≤ 50 μg/m L and ≤ 200 μg/m L was observed whereas, hydrogen peroxide scavenging activity with IC_(50)values of ≤25 μg/m L for aerial parts and ≤ 50 μg/m L for the root extract of Lespedeza bicolor has been shown.CONCLUSION: These data suggested that the methanolic extract of Lespedeza bicolor could be potential candidates for natural antioxidants and anticancer.
