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Diagnosis and Prevention of Avian Leukosis
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作者 Hu Xiaomiao Dai Yin +7 位作者 Pan Xiaocheng Shen Xuehuai Zhou Xueli Zhao Ruihong Hou Hongyan Yin Lei Yin Dongdong Liu Yayun 《Animal Husbandry and Feed Science》 CAS 2023年第1期25-29,共5页
This paper introduced the characteristics of avian leukosis from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and laboratory diagnost... This paper introduced the characteristics of avian leukosis from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and laboratory diagnostic methods.Besides,the disease is also differentiated from similar diseases of chicken such as Marek's disease,infectious bursal disease and reticuloendothelial hyperplasia.Finally,the prevention and control measures against the disease were proposed. 展开更多
关键词 Avian leukosis PATHOGEN Epidemiology:Clinical symptom Anatomical symptom Diagnosis method Prevention and control measure
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Isolation and Identification of a Subgroup A Avian Leukosis Virus from Imported Meat-type Grand-parent Chickens 被引量:28
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作者 Qing-chan ZHANG Dong-min ZHAO Hui-jun GUO Zhi-zhong CUI 《Virologica Sinica》 SCIE CAS CSCD 2010年第2期130-136,共7页
An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced a... An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that SDAU09C1 fell into subgroup A; it had homology of 88.8%-90.3% to 6 reference strains of subgroup A, much higher compared to other subgroups including subgroup B. This is the first report for ALV of subgroup A isolated from imported breeders. 展开更多
关键词 Avian leukosis virus(ALV) Subgroup A Envelope gp85 Imported Chicken Breeders
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Establishment of an avian leukosis virus subgroup A-resistant cell line 被引量:4
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作者 FENG Min DAI Man-man +1 位作者 LIAO Ming CAO Wei-sheng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第4期930-936,共7页
Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A (A... Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A (ALV-A) env gene into plasmid pcDNA3.1/Zeo (+) and used this construct to transfect DF-1 cells. Zeocin-resistant cells were obtained after 2 weeks of zeocin selection. Then, the cells were analyzed using PCR, immunofluorescence, and Western blot for expression of the envA-encoded envelope protein after 30 serial passages. The DF-1/A cell line was completely resistant to 104 TCIDso/0.1 mL (50% tissue culture infective dose)ALV-A and was partially resistant to 10~ TCIDs0/0.1 mL ALV-A viral particles. By comparing the DF-1/A and DF-1 cell lines, an ALV-A isolate was identified using a gag-specific ELISAfor capsid protein p27. Thus, we established a DF-1/A cell line that was resistant to ALV-A infection. This cell line will be useful as a diagnostic tool. 展开更多
关键词 avian leukosis virus subgroup A (ALV-A) ENV DF-1/A cell line diagnostic tool
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Study on the Pathogenicity of Chinese Strains of Subgroup J Avian Leukosis Viruses 被引量:2
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作者 DU Yan and GUI Zhi-zhong(College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018 , P.R. China) 《Agricultural Sciences in China》 CAS CSCD 2002年第5期585-588,共4页
The pathogenicity of 4 Chinese strains of subgroup J avian leukosis viruses (ALV-J), SD9901, SD9902, YZ9901 and YZ9902,was studied. The results showed that only SD9902 among the 4 strains induced mortality from myeloi... The pathogenicity of 4 Chinese strains of subgroup J avian leukosis viruses (ALV-J), SD9901, SD9902, YZ9901 and YZ9902,was studied. The results showed that only SD9902 among the 4 strains induced mortality from myeloid leukosis (ML). In the 12 meat-type chickens inoculated with SD9902 at 1-day-old, 9 died between 22 days and 38 days after inoculation. No death or ML was found in chickens inoculated with the other 3 strains during the period of 6 months. These results suggested the SD9902 strain of ALV-J was an acute transforming virus, but SD9901, YZ9901 and YZ9902 were non-transforming viruses. All 4 Chinese strains did not induce any tumors in egg-type SPF chickens during 7 months after hatching when viruses were injected into 11-day-old embryos. 展开更多
关键词 ALV-J PATHOGENICITY Myeloid leukosis
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Different quasispecies with great mutations hide in the same subgroup J field strain of avian leukosis virus 被引量:6
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作者 MAO YaQing LI WeiHua +2 位作者 DONG Xuan LIU JinHua ZHAO Peng 《Science China(Life Sciences)》 SCIE CAS 2013年第5期414-420,共7页
Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and ... Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and identification, respectively. The inoculated cells were screened for three common chicken tumor viruses. Nine strains of avian leukosis virus subgroup J (ALV-J) were identified, and were designated LY1201-LYI209. The env gene from the LY1201 strain was amplified and cloned. All nine resultant env clones (clones 01-09) were sequenced, and the gp85 and gp37 amino acid regions were subjected to homology analysis. Clones 01 and 03 had 10 amino acid deletions in the gp85 region compared to the other seven clones, suggesting that at least two quasispecies with obvious mutations coexist in the same field strain. Among these nine clones, three had identical gp85 and gp37 sequences, and were recognized as the dominant LY1201 quasispecies. The amino acid sequence homology of gp37 and gp85 among the nine clones was 98.5%-100.0% and 96.6%-100.0% respectively, suggesting that the gp85 region of the env gene can better display the quasispecies diversity of ALV-J than gp37. 展开更多
关键词 avian leukosis virus subgroup J(ALV-J) gp85 gp37 quasispecies diversity
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Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus
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作者 Liqin Liao Weiguo Chen +9 位作者 Xiangyu Zhang Huanmin Zhang Aijun Li Yiming Yan Zi Xie Hongxing Li Wencheng Lin Jingyun Ma Xinheng Zhang Qingmei Xie 《Virologica Sinica》 SCIE CAS CSCD 2022年第2期284-294,共11页
Subgroup J avian leukosis virus(ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is... Subgroup J avian leukosis virus(ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles(EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles(SE) were isolated and purified from ALV-J-infected rooster seminal plasma(SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection.More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission. 展开更多
关键词 Semen extracellular vesicles(SE) Subgroup J avian leukosis virus(ALV-J) INFECTION Vertical transmission
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Advances on genetic and genomic studies of ALV resistance 被引量:1
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作者 Guodong Mo Ping Wei +2 位作者 Bowen Hu Qinghua Nie Xiquan Zhang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第1期1-14,共14页
Avian leukosis(AL)is a general term for a variety of neoplastic diseases in avian caused by avian leukosis virus(ALV).No vaccine or drug is currently available for the disease.Therefore,the disease can result in sever... Avian leukosis(AL)is a general term for a variety of neoplastic diseases in avian caused by avian leukosis virus(ALV).No vaccine or drug is currently available for the disease.Therefore,the disease can result in severe economic losses in poultry flocks.Increasing the resistance of poultry to ALV may be one effective strategy.In this review,we provide an overview of the roles of genes associated with ALV infection in the poultry genome,including endogenous retroviruses,virus receptors,interferon-stimulated genes,and other immune-related genes.Furthermore,some methods and techniques that can improve ALV resistance in poultry are discussed.The objectives are willing to provide some valuable references for disease resistance breeding in poultry. 展开更多
关键词 Avian leukosis Endogenous retrovirus Gene editing IMMUNITY Interferon-stimulated genes Receptor Resistant breeding
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Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B, and J 被引量:5
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作者 Hong Jo Lee Kyung Je Park +3 位作者 Kyung Youn Lee Yongxiu Yao Venugopal Nair Jae Yong Han 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2019年第2期410-423,共14页
Background: Previously, we showed that targeted disruption of viral receptor genes in avian leukosis virus(ALV)subgroups using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protei... Background: Previously, we showed that targeted disruption of viral receptor genes in avian leukosis virus(ALV)subgroups using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9))-based genome editing confers resistance to ALV subgroups B and J. Here, we used the same strategy to target the receptor expressed by ALV subgroup A(TVA) and generate chicken cells resistant to infection by this virus.Results: CRISPR/Cas9-based disruption of exon 2 within the tva gene of DF-1 fibroblasts conferred resistance to infection by ALV subgroup A regardless of whether frameshift mutations were introduced during editing. Conversely,overexpression of the wild-type TVA receptor(wtTVA) by tva-modified DF-1 clones restored susceptibility to ALV subgroup A. The results confirm that exon 2, which contains the low-density lipoprotein receptor class A domain of TVA, is critical for virus entry. Furthermore, we sequentially modified DF-1 cells by editing the tva, tvb, and Na^+/H^+ exchange 1(chNHE1) genes, which are the specific receptors for ALV subgroups A, B, and J, respectively.Conclusions: Simultaneous editing of multiple receptors to block infection by different subgroups of ALV confirmed that ALV subgroups A, B, and J do not share host receptors. This strategy could be used to generate cells resistant to multiple viral pathogens that use distinct receptors for cell entry. 展开更多
关键词 Avian leukosis virus CRISPR/Cas9 Genome editing HOST receptor TVA
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Influence of REV and ALV-J Co-Infection on Immunologic Function of T Lymphocytes and Histopathology in Broiler Chickens 被引量:6
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作者 GUO Hui-jun, LI Hong-mei, CHENG Zi-qiang , LIU Jian-zhu and CUI Zhi-zhong Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veternary Medicine, Shandong Agricultural University, Tai’an 271018, P.R.China 《Agricultural Sciences in China》 CSCD 2010年第11期1667-1676,共10页
The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lym... The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lymphocytes including lymphoproliferation responses, cytotoxicitic responses, and histopathology of spleen were detected in broiler chickens singly- or co-infected with REV and ALV-J at different days post inoculation and the virus expressions in spleen of infected broiler chickens were detected with immunofluorescence assay (IFA). The results indicated that blood and spleen T lymphocytes proliferation responses and cytotoxicity in broilers infected with REV or/and ALV-J were inhibited in the whole observed period compared with controls. In the co-infected chickens they were highly inhibited than in the single-infected. The histopathology of spleen in infected chickens at 17 and 37 d post inoculation (dpi) indicated that cell interium increased, the numbers of lymphocytes decreased, and the regrowth were destroyed or decreased, especially more significantly at 17 than at 37 dpi. The different numbers of virus were detected in spleen lymphocytes in REV- infected and/or ALV-J-infected chickens. In the spleen of co-infected chicken, both REV and ALV-J were detected and the total numbers of viruses were more than in chickens singly-infected with REV or ALV-J. Thus, the co-effect of REV and ALV-J caused more immunosuppression on T lymphocytes bioactivities in broiler chickens than single-effect of ALV-J or REV, which contributed to the sever histopathology and the product of tumor cells. This study will be helpful for understanding the effect of co-infection with many viruses and control them in poultry. 展开更多
关键词 reticuloendotheliosis virus (REV) J subgroup of avian leukosis virus (ALV-J) T lymphocyte activity histopathology commercial broiler chickens
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