目的探讨血清微小核糖核酸-503(miR-503)和微小核糖核酸-520h(miR-520h)对妊娠期糖尿病(GDM)患者并发子痫前期(PE)的诊断价值。方法选取2019年12月至2021年12月于唐山中心医院产科定期产检并建档的GDM患者102例作为研究对象,根据疾病类...目的探讨血清微小核糖核酸-503(miR-503)和微小核糖核酸-520h(miR-520h)对妊娠期糖尿病(GDM)患者并发子痫前期(PE)的诊断价值。方法选取2019年12月至2021年12月于唐山中心医院产科定期产检并建档的GDM患者102例作为研究对象,根据疾病类型分为GDM+PE组(53例)、GDM组(49例)。另选取同期于唐山中心医院进行定期产检并分娩的57例健康孕妇作为正常妊娠组。比较3组糖脂代谢相关指标[甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、游离脂肪酸(FFA)、空腹胰岛素(FINS)、空腹血糖(FBG)、餐后2 h血糖(2 h PG)]水平及血清miR-503、miR-520h表达水平、血清胱抑素C(CysC)、同型半胱氨酸(Hcy)水平;比较3组不良妊娠结局。采用多因素Logistic回归分析GDM患者并发PE的危险因素;绘制受试者工作特征(ROC)曲线分析miR-503、miR-520h单独及2项指标联合检测对GDM并发PE的诊断价值。结果3组孕周、年龄、孕前体质量指数、LDL-C水平比较,差异均无统计学意义(P>0.05)。GDM+PE组TC、TG、FFA水平均明显高于GDM组和正常妊娠组,HDL-C水平明显低于GDM组和正常妊娠组,差异均有统计学意义(P<0.05)。GDM组TC、FFA水平均明显高于正常妊娠组,差异均有统计学意义(P<0.05)。GDM+PE组和GDM组FINS、FBG、2 h PG、HbA1c水平均明显高于正常妊娠组,差异均有统计学意义(P<0.05)。GDM+PE组血清miR-503、miR-520h表达水平、CysC和Hcy水平均明显高于GDM组和正常妊娠组,差异均有统计学意义(P<0.05),GDM组血清miR-503、miR-520h表达水平及CysC和Hcy水平均明显高于正常妊娠组,差异均有统计学意义(P<0.05)。GDM+PE组不良妊娠结局总发生率为62.26%(33/53),GDM组为40.82%(20/49),正常妊娠组为10.53%(6/57),GDM+PE组不良妊娠结局总发生率明显高于GDM组和正常妊娠组,差异均有统计学意义(χ^(2)=4.692、32.125,P<0.05),GDM组不良妊娠结局总发生率明显高于正常妊娠组,差异有统计学意义(χ^(2)=13.059,P<0.05)。3组孕妇不良妊娠结局中产后出血、早产或过期产、胎盘早剥、新生儿窒息、宫内生长受限的发生率比较,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,CysC水平升高、Hcy水平升高、miR-503表达水平升高和miR-520h表达水平升高均为GDM并发PE的危险因素(P<0.05)。ROC曲线分析结果显示,血清miR-503、miR-520h单独及2项指标联合检测诊断GDM并发PE的曲线下面积(AUC)分别为0.755、0.847、0.935,2项指标联合检测的AUC大于miR-503、miR-520h单独检测(Z_(2项联合-miR-503)=4.210、Z_(2项联合-miR-520h)=2.708,P<0.001、0.007)。结论GDM并发PE患者血清miR-503、miR-520h表达水平升高,与妊娠不良结局呈正相关,二者联合检测对诊断GDM并发PE具有重要意义。展开更多
目的 探讨原发性肝癌(primary carcinoma of the liver)患者血清微小核糖核酸(microRNA,miR)-196b,微小核糖核酸(microRNA,miR)-520f表达水平及其临床诊断价值。方法 选择2020年6月~2022年6月攀枝花学院附属医院收治的73例原发性肝癌患...目的 探讨原发性肝癌(primary carcinoma of the liver)患者血清微小核糖核酸(microRNA,miR)-196b,微小核糖核酸(microRNA,miR)-520f表达水平及其临床诊断价值。方法 选择2020年6月~2022年6月攀枝花学院附属医院收治的73例原发性肝癌患者作为研究组,患者均符合《原发性肝癌诊疗规范》中的诊断标准;选择同期医院体检的80例健康人群作为对照组。抽取研究对象清晨空腹外周静脉血5ml,采用实时荧光定量逆转录-聚合酶链反应(real time fluorescent quantitative reverse transcription-polymerase chain reaction,qRT-PCR)检测两组血清miR-196b和miR-520f表达水平。分析不同临床病理特征原发性肝癌患者血清miR-196b和miR-520f表达水平差异,采用Pearson相关性分析探讨血清miR-196b与miR-520f的关系,采用受试者工作特征(receiver operating characteristic,ROC)曲线分析血清miR-196b和miR-520f对原发性肝癌的诊断价值。结果 研究组血清miR-196b表达水平(2.73±0.56)明显高于对照组(0.99±0.24),miR-520f表达水平(1.69±0.44)明显低于对照组(3.34±0.64),差异具有统学意义(t=30.578,12.690,均P<0.05)。与Ⅰ~Ⅱ期、中高分化、无淋巴结转移、肿瘤直径<5cm患者相比,Ⅲ~Ⅳ期、低分化、淋巴结转移和肿瘤直径≥5cm患者血清miR-196b表达水平均升高,miR-520f表达水平均降低,差异具有统计学意义(tmiR-196b=6.919~13.229,tmiR-520f=3.873~7.814,均P<0.05)。Pearson相关性分析显示,原发性肝癌患者血清miR-196b表达水平与miR-520f表达水平呈负相关(r=-0.445,P=0.006)。ROC曲线分析显示,血清miR-196b预测原发性肝癌的曲线下面积、截断值、敏感度、特异度及95%置信区间(95%confidence interval,95%CI)分别为0.842,2.55,91.78%,62.50%和95%CI(0.810~0.874);miR-520f预测原发性肝癌的的曲线下面积、截断值、敏感度、特异度及95%置信区间分别为0.872,2.43,91.78%,67.50%和95%CI(0.848~0.906);血清miR-196b联合miR-520f预测原发性肝癌的曲线下面积、敏感度、特异度及95%置信区间分别为0.923,86.30%,87.50%和95%CI(0.891~0.955)。结论 原发性肝癌患者血清miR-196b表达水平上调,miR-520f表达水平下调,其表达水平变化与肿瘤直径、临床分期、分化程度和淋巴结转移密切相关,联合检测血清miR-196b与miR-520f能有效提高原发性肝癌的诊断效能。展开更多
Background:Colorectal cancer(CRC)is one of the most common malignancies.Early diagnosis is the key to effective treatment of CRC.Since microRNAs(miRNAs)can be used as biomarkers of CRC,the objective of this work was t...Background:Colorectal cancer(CRC)is one of the most common malignancies.Early diagnosis is the key to effective treatment of CRC.Since microRNAs(miRNAs)can be used as biomarkers of CRC,the objective of this work was to examine the effect of miR-520f-3p,which targets YAP1(Yes-associated protein 1),on the ability of CRC cells to proliferate,invade,migrate,and undergo epithelial-mesenchymal transition(EMT).Methods:A miR-520f-3p mimic was used to overexpress miR-520f-3p in HT29 cells.To establish the tumor-bearing mouse model,transfected HT29 cells were subcutaneously implanted into BALB/c-nu nude mice,and YAP1 and miR-520f-3p levels were determined using qRT‒PCR.The viability,invasion ability,and migration ability of cells were evaluated by CCK-8,Transwell,and wound healing assays.Apoptosis was detected by flow cytometry and TUNEL assays.The regulatory link between miR-520f-3p and the YAP1 gene was examined by dual-luciferase reporter assay.Tumor tissues with positive Ki-67 expression were identified by immunohistochemistry.Vimentin,E-cadherin,and YAP1 expression were evaluated by western blotting.Results:MiR-520f-3p overexpression could inhibit proliferation,invasion,migration,and EMT and induce apoptosis in HT29 cells.YAP1 was found as a target of miR-520f-3p.The inhibitory effects of miR-520f-3p on proliferation,invasion,migration,and EMT may be reversed by overexpressing YAP1.In tumor-bearing mice,miR-520f-3p overexpression reduced the Ki-67 level,increased apoptosis,and prevented tumor development and spread.Conclusion:By targeting YAP1,miR-520f-3p may be capable of suppressing CRC cell proliferation,invasion,migration,and EMT,providing a novel therapeutic target for the disease.展开更多
文摘目的探讨血清微小核糖核酸-503(miR-503)和微小核糖核酸-520h(miR-520h)对妊娠期糖尿病(GDM)患者并发子痫前期(PE)的诊断价值。方法选取2019年12月至2021年12月于唐山中心医院产科定期产检并建档的GDM患者102例作为研究对象,根据疾病类型分为GDM+PE组(53例)、GDM组(49例)。另选取同期于唐山中心医院进行定期产检并分娩的57例健康孕妇作为正常妊娠组。比较3组糖脂代谢相关指标[甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、游离脂肪酸(FFA)、空腹胰岛素(FINS)、空腹血糖(FBG)、餐后2 h血糖(2 h PG)]水平及血清miR-503、miR-520h表达水平、血清胱抑素C(CysC)、同型半胱氨酸(Hcy)水平;比较3组不良妊娠结局。采用多因素Logistic回归分析GDM患者并发PE的危险因素;绘制受试者工作特征(ROC)曲线分析miR-503、miR-520h单独及2项指标联合检测对GDM并发PE的诊断价值。结果3组孕周、年龄、孕前体质量指数、LDL-C水平比较,差异均无统计学意义(P>0.05)。GDM+PE组TC、TG、FFA水平均明显高于GDM组和正常妊娠组,HDL-C水平明显低于GDM组和正常妊娠组,差异均有统计学意义(P<0.05)。GDM组TC、FFA水平均明显高于正常妊娠组,差异均有统计学意义(P<0.05)。GDM+PE组和GDM组FINS、FBG、2 h PG、HbA1c水平均明显高于正常妊娠组,差异均有统计学意义(P<0.05)。GDM+PE组血清miR-503、miR-520h表达水平、CysC和Hcy水平均明显高于GDM组和正常妊娠组,差异均有统计学意义(P<0.05),GDM组血清miR-503、miR-520h表达水平及CysC和Hcy水平均明显高于正常妊娠组,差异均有统计学意义(P<0.05)。GDM+PE组不良妊娠结局总发生率为62.26%(33/53),GDM组为40.82%(20/49),正常妊娠组为10.53%(6/57),GDM+PE组不良妊娠结局总发生率明显高于GDM组和正常妊娠组,差异均有统计学意义(χ^(2)=4.692、32.125,P<0.05),GDM组不良妊娠结局总发生率明显高于正常妊娠组,差异有统计学意义(χ^(2)=13.059,P<0.05)。3组孕妇不良妊娠结局中产后出血、早产或过期产、胎盘早剥、新生儿窒息、宫内生长受限的发生率比较,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,CysC水平升高、Hcy水平升高、miR-503表达水平升高和miR-520h表达水平升高均为GDM并发PE的危险因素(P<0.05)。ROC曲线分析结果显示,血清miR-503、miR-520h单独及2项指标联合检测诊断GDM并发PE的曲线下面积(AUC)分别为0.755、0.847、0.935,2项指标联合检测的AUC大于miR-503、miR-520h单独检测(Z_(2项联合-miR-503)=4.210、Z_(2项联合-miR-520h)=2.708,P<0.001、0.007)。结论GDM并发PE患者血清miR-503、miR-520h表达水平升高,与妊娠不良结局呈正相关,二者联合检测对诊断GDM并发PE具有重要意义。
文摘Background:Colorectal cancer(CRC)is one of the most common malignancies.Early diagnosis is the key to effective treatment of CRC.Since microRNAs(miRNAs)can be used as biomarkers of CRC,the objective of this work was to examine the effect of miR-520f-3p,which targets YAP1(Yes-associated protein 1),on the ability of CRC cells to proliferate,invade,migrate,and undergo epithelial-mesenchymal transition(EMT).Methods:A miR-520f-3p mimic was used to overexpress miR-520f-3p in HT29 cells.To establish the tumor-bearing mouse model,transfected HT29 cells were subcutaneously implanted into BALB/c-nu nude mice,and YAP1 and miR-520f-3p levels were determined using qRT‒PCR.The viability,invasion ability,and migration ability of cells were evaluated by CCK-8,Transwell,and wound healing assays.Apoptosis was detected by flow cytometry and TUNEL assays.The regulatory link between miR-520f-3p and the YAP1 gene was examined by dual-luciferase reporter assay.Tumor tissues with positive Ki-67 expression were identified by immunohistochemistry.Vimentin,E-cadherin,and YAP1 expression were evaluated by western blotting.Results:MiR-520f-3p overexpression could inhibit proliferation,invasion,migration,and EMT and induce apoptosis in HT29 cells.YAP1 was found as a target of miR-520f-3p.The inhibitory effects of miR-520f-3p on proliferation,invasion,migration,and EMT may be reversed by overexpressing YAP1.In tumor-bearing mice,miR-520f-3p overexpression reduced the Ki-67 level,increased apoptosis,and prevented tumor development and spread.Conclusion:By targeting YAP1,miR-520f-3p may be capable of suppressing CRC cell proliferation,invasion,migration,and EMT,providing a novel therapeutic target for the disease.
