In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen wit...In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.展开更多
The Lanzhou Institute of Physics CAST completed the unit performance test of the flight model of LHT-100 Hal thruster as well as the joint testing of the full system of the Hall electric propulsion subsystem.This was ...The Lanzhou Institute of Physics CAST completed the unit performance test of the flight model of LHT-100 Hal thruster as well as the joint testing of the full system of the Hall electric propulsion subsystem.This was a comprehensive test on the Hall electric propulsion展开更多
Using a degenerative probe designed according to the most conservative region of a known Lys- and His-specific amino acid transporter (LHT1) from Arabidopsis, we isolated a full-length cDNA named OxHT(histidine transp...Using a degenerative probe designed according to the most conservative region of a known Lys- and His-specific amino acid transporter (LHT1) from Arabidopsis, we isolated a full-length cDNA named OxHT(histidine transporter of Oryza sativa L.) by screening the rice cDNA library. The cDNA is 1.3 kb in length and the open reading frame encodes for a 441 amino acid protein with a calculated molecular mass of 49 kDa. Multiple sequence alignments showed that OsHT shares a high degree of sequence conservation at the deduced amino acid level with the Arabidopsis LHT1 and six putative lysine and histidine transporters. Computational analysis indicated that OsHT is an integral membrane protein with 11 putative transmembrane helices. This was confirmed by the transient expression assay because the OsHT-GFP fusion protein was, indeed, localized mainly in the plasma membrane of onion epidermal cells. Functional complementation experiments demonstrated that OsHT was able to work as a histidine transporter in Saccharomyces cerevisiae, suggesting that OsHT is a gene that encodes for a histidine transporter from rice. This is the first time that an LHT-type amino acid transporter gene has been cloned from higher plants other than Arabidopsis.展开更多
We perform a comparative study for the LHC di-photon Higgs signal in three different littlest Higgs models with T-parity (named LHT-Ⅰ, LHT-Ⅱ and LHT-Ⅲ). We find that compared with the Standard Model prediction, the...We perform a comparative study for the LHC di-photon Higgs signal in three different littlest Higgs models with T-parity (named LHT-Ⅰ, LHT-Ⅱ and LHT-Ⅲ). We find that compared with the Standard Model prediction, the di-photon rate is always suppressed and the suppression extent can be different for different models. The LHC will soon give a solid judgement whether the di-photon rate is larger than the SM prediction, which can be utilized as a sensitive probe for the three models.展开更多
Considering the process pp→VH ＋X（V = W or Z） is a significant channel for searching for a light Higgs boson, we calculate the contributions of the littlest Higgs model with T-parity （called LHT model） to its pro...Considering the process pp→VH ＋X（V = W or Z） is a significant channel for searching for a light Higgs boson, we calculate the contributions of the littlest Higgs model with T-parity （called LHT model） to its production cross section. We find that, in most of the parameter space, the value of the relative correction parameter R is very small. However, with reasonable values of the free parameters, its value can be significantly larger.展开更多
Based on calculating the contributions of the littlest Higgs model with T-parity （called LHT model） to the anomalous top coupling tqγ （q = u or c）, we consider single top production via the t-channel partonic pro...Based on calculating the contributions of the littlest Higgs model with T-parity （called LHT model） to the anomalous top coupling tqγ （q = u or c）, we consider single top production via the t-channel partonic process eq→et in ep collisions. Our numerical results show that the production cross section in the LHT model can be significantly enhanced relative to that in the standard model （SM）.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2018YFD1000600)the National Natural Science Foundation of China(Grant No.32070376)。
文摘In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.
文摘The Lanzhou Institute of Physics CAST completed the unit performance test of the flight model of LHT-100 Hal thruster as well as the joint testing of the full system of the Hall electric propulsion subsystem.This was a comprehensive test on the Hall electric propulsion
文摘Using a degenerative probe designed according to the most conservative region of a known Lys- and His-specific amino acid transporter (LHT1) from Arabidopsis, we isolated a full-length cDNA named OxHT(histidine transporter of Oryza sativa L.) by screening the rice cDNA library. The cDNA is 1.3 kb in length and the open reading frame encodes for a 441 amino acid protein with a calculated molecular mass of 49 kDa. Multiple sequence alignments showed that OsHT shares a high degree of sequence conservation at the deduced amino acid level with the Arabidopsis LHT1 and six putative lysine and histidine transporters. Computational analysis indicated that OsHT is an integral membrane protein with 11 putative transmembrane helices. This was confirmed by the transient expression assay because the OsHT-GFP fusion protein was, indeed, localized mainly in the plasma membrane of onion epidermal cells. Functional complementation experiments demonstrated that OsHT was able to work as a histidine transporter in Saccharomyces cerevisiae, suggesting that OsHT is a gene that encodes for a histidine transporter from rice. This is the first time that an LHT-type amino acid transporter gene has been cloned from higher plants other than Arabidopsis.
基金supported by the National Natural Science Foundation of China (Grant Nos. 11105116 and 11005089)
文摘We perform a comparative study for the LHC di-photon Higgs signal in three different littlest Higgs models with T-parity (named LHT-Ⅰ, LHT-Ⅱ and LHT-Ⅲ). We find that compared with the Standard Model prediction, the di-photon rate is always suppressed and the suppression extent can be different for different models. The LHC will soon give a solid judgement whether the di-photon rate is larger than the SM prediction, which can be utilized as a sensitive probe for the three models.
基金Supported by National Natural Science Foundation of China (10975067)Specialized Research Fund for Doctoral Program of Higher Education (SRFDP) (200801650002)+1 种基金Natural Science Foundation of Liaoning Science Committee (20082148)Foundation of Liaoning Educational Committee (2007T086)
文摘Considering the process pp→VH ＋X（V = W or Z） is a significant channel for searching for a light Higgs boson, we calculate the contributions of the littlest Higgs model with T-parity （called LHT model） to its production cross section. We find that, in most of the parameter space, the value of the relative correction parameter R is very small. However, with reasonable values of the free parameters, its value can be significantly larger.
基金Supported by NSFC (10675057)Foundation of Liaoning Educational Committee (2007T086)
文摘Based on calculating the contributions of the littlest Higgs model with T-parity （called LHT model） to the anomalous top coupling tqγ （q = u or c）, we consider single top production via the t-channel partonic process eq→et in ep collisions. Our numerical results show that the production cross section in the LHT model can be significantly enhanced relative to that in the standard model （SM）.
