Objective To explore the pharmacodynamic mechanism of Fu Zi Li Zhong Decoction(FZLZD)in treating gastric ulcer and its effect on gastrointestinal microecology.Methods Rat gastric ulcer model was established by continu...Objective To explore the pharmacodynamic mechanism of Fu Zi Li Zhong Decoction(FZLZD)in treating gastric ulcer and its effect on gastrointestinal microecology.Methods Rat gastric ulcer model was established by continuous vinegar gavage.The experiment included normal group,model group,ranitidine group and FZLZD group.The animals were handled after intragastricing administration of corresponding clinical isodose drugs for 7 d.The rats’gastrointestinal histopathology was observed by HE staining.The expression of ZO-1 and Occludin mRNA in rat gastric tissue was detected by RT-PCR.The diversity of intestinal flora was analyzed by 16SrDNA sequencing.Results FZLZD can effectively improve gastric mucosal destruction,epithelial exfoliation,inflammatory cell infiltration,inflammatory substance exudation and edema in rats with gastric ulcer.FZLZD can effectively up-regulate the expression levels of ZO-1 and Occludin mRNA in gastric tissue of rats with gastric ulcer.The results of intestinal flora diversity showed that the diversity of intestinal flora in FZLZD group was significantly higher than that of model group.Conclusions FZLZD can regulate gastric ulcer by promoting the expression of ZO-1 and Occludin and improving the diversity of intestinal flora.展开更多
基金funding support from the National Natural Science Foundation of China (No. 81973670 and No. 81503445)Hunan Provincial Natural Science Foundation of China (No. 2016JJ2095)Hunan Province College Students Research Learning and Innovative Experiment Project (No. 2018-412)
文摘Objective To explore the pharmacodynamic mechanism of Fu Zi Li Zhong Decoction(FZLZD)in treating gastric ulcer and its effect on gastrointestinal microecology.Methods Rat gastric ulcer model was established by continuous vinegar gavage.The experiment included normal group,model group,ranitidine group and FZLZD group.The animals were handled after intragastricing administration of corresponding clinical isodose drugs for 7 d.The rats’gastrointestinal histopathology was observed by HE staining.The expression of ZO-1 and Occludin mRNA in rat gastric tissue was detected by RT-PCR.The diversity of intestinal flora was analyzed by 16SrDNA sequencing.Results FZLZD can effectively improve gastric mucosal destruction,epithelial exfoliation,inflammatory cell infiltration,inflammatory substance exudation and edema in rats with gastric ulcer.FZLZD can effectively up-regulate the expression levels of ZO-1 and Occludin mRNA in gastric tissue of rats with gastric ulcer.The results of intestinal flora diversity showed that the diversity of intestinal flora in FZLZD group was significantly higher than that of model group.Conclusions FZLZD can regulate gastric ulcer by promoting the expression of ZO-1 and Occludin and improving the diversity of intestinal flora.
