The physiological performance of a mid-intertidal limpet Cellana toreuma was determined to study the physiological adaptation of intertidal animals to rapid changes and extreme temperatures during emersion.The relatio...The physiological performance of a mid-intertidal limpet Cellana toreuma was determined to study the physiological adaptation of intertidal animals to rapid changes and extreme temperatures during emersion.The relationship between the Arrhenius breakpoint temperature(ABT)and in situ operative body temperature was studied to predict the possible impact of climate change on the species.The temperature coefficient(Q10)of emersed animals was higher than that of submersed animals and the ratio of aerial:aquatic heart rate rose with increasing temperature.The ABTs of submersed and emersed animals were 30.2 and 34.2°C,respectively.The heart rate and levels of molecular biomarkers(hsps,ampkα,ampkβand sirt1 mRNA)were determined in 48 h simulated semi-diurnal tides.There were no obvious changes of heart rate and gene expression during the transition between emersion and submersion at room temperature,although expressions of hsp70 and hsp90 were induced significantly after thermal stress.These results indicate that C.toreuma can effectively utilize atmospheric oxygen,and the higher Q10 and ABT of emersed animals are adaptations to the rapid change and extreme thermal stress during emersion.However,the in situ operative body temperature frequently exceeds the aerial ABT of C.toreuma,indicating the occurrence of large-scale mortality of C.toreuma in summer,and this species should be sensitive to increasing temperature in the scenario of climate change.展开更多
The predictability of high temperatures is important for intertidal species for coping with thermal stress.To investigate the roles of high temperature and its predictability on the population abundance and reproducti...The predictability of high temperatures is important for intertidal species for coping with thermal stress.To investigate the roles of high temperature and its predictability on the population abundance and reproductive output of an intertidal rocky shore limpet,Siphonaria japonica,we monitored the operative temperature,recorded the population abundance of egg ribbons and adults,and quantified the expression of heat shock protein 70(hsp70)mRNA on two warm-temperate rocky shores with different thermal environments.Abundances of limpets and egg ribbons in the hotter but more predictable(HP)habitats were higher than those in the relatively benign and unpredictable(BU)habitats.In the HP habitats,there was a strong positive correlation between habitat temperature and population abundance.For limpets living in the HP habitats,the expression of hsp70 exhibited a smaller increase with rising body temperature than for BU limpets,indicating the existence in HP limpets of a preparatory strategy in cellular stress response against thermal stress.Our results dem on strate that the predictability of operative temperature can affect physiological responses and population dynamics.The importance of predictability should be considered in analyses of the ecological consequences of climate warming.展开更多
以钥孔戚血蓝蛋白(Keyhole limpet hemocyanin,KLH)为抗原、Montanide ISA 763 A为佐剂开展金鱼免疫试验,检测血清抗体水平,评估金鱼体液免疫应答情况,以期为其免疫策略的制定提供依据。试验在水温15~18℃时进行,采用(20±3)g体重...以钥孔戚血蓝蛋白(Keyhole limpet hemocyanin,KLH)为抗原、Montanide ISA 763 A为佐剂开展金鱼免疫试验,检测血清抗体水平,评估金鱼体液免疫应答情况,以期为其免疫策略的制定提供依据。试验在水温15~18℃时进行,采用(20±3)g体重的健康鱼150尾,随机均分为三组,第一组使用(KLH+Montanide ISA 763 A)免疫,第二组仅使用KLH免疫,第三组注射等量的0.01 mol/L磷酸盐缓冲液(Phosphate buffered solution,PBS)为对照,历经三次免疫并对4次采样的血清样本进行ELISA检测。结果显示,第一组和第二组均检测到较高的抗KLH抗体水平,在第二次、第三次免疫后,添加ISA佐剂的第一组抗体仍维持较高水平,而在仅用KLH免疫的第二组抗体水平下降,并降低到对照组水平,表明使用(KLH+Montanide ISA 763 A)免疫金鱼可以获得更加持久和稳定的免疫效果,而仅注射KLH的免疫效果较为短暂。研究还说明金鱼在低温情况下,可以产生良好的体液免疫应答。展开更多
基金supported by grants from Nature Science funds for Distinguished Young Scholars of Fujian Province,China(2011J06017)National Natural Science Foundation of China(41076083,41276126)the Fundamental Research Funds for the Central Universities and the Program for New Century Excellent Talents in University of Fujian Province.We thank Dr Colin Little for constructive discussions and help with preparation of the manuscript.
文摘The physiological performance of a mid-intertidal limpet Cellana toreuma was determined to study the physiological adaptation of intertidal animals to rapid changes and extreme temperatures during emersion.The relationship between the Arrhenius breakpoint temperature(ABT)and in situ operative body temperature was studied to predict the possible impact of climate change on the species.The temperature coefficient(Q10)of emersed animals was higher than that of submersed animals and the ratio of aerial:aquatic heart rate rose with increasing temperature.The ABTs of submersed and emersed animals were 30.2 and 34.2°C,respectively.The heart rate and levels of molecular biomarkers(hsps,ampkα,ampkβand sirt1 mRNA)were determined in 48 h simulated semi-diurnal tides.There were no obvious changes of heart rate and gene expression during the transition between emersion and submersion at room temperature,although expressions of hsp70 and hsp90 were induced significantly after thermal stress.These results indicate that C.toreuma can effectively utilize atmospheric oxygen,and the higher Q10 and ABT of emersed animals are adaptations to the rapid change and extreme thermal stress during emersion.However,the in situ operative body temperature frequently exceeds the aerial ABT of C.toreuma,indicating the occurrence of large-scale mortality of C.toreuma in summer,and this species should be sensitive to increasing temperature in the scenario of climate change.
基金The study was support by National Natural Science Foundation of China(nos.41776135,41976142)Nature Science funds fbr Distinguished Young Scholars of Fujian Province,China(no.2017J07003).
文摘The predictability of high temperatures is important for intertidal species for coping with thermal stress.To investigate the roles of high temperature and its predictability on the population abundance and reproductive output of an intertidal rocky shore limpet,Siphonaria japonica,we monitored the operative temperature,recorded the population abundance of egg ribbons and adults,and quantified the expression of heat shock protein 70(hsp70)mRNA on two warm-temperate rocky shores with different thermal environments.Abundances of limpets and egg ribbons in the hotter but more predictable(HP)habitats were higher than those in the relatively benign and unpredictable(BU)habitats.In the HP habitats,there was a strong positive correlation between habitat temperature and population abundance.For limpets living in the HP habitats,the expression of hsp70 exhibited a smaller increase with rising body temperature than for BU limpets,indicating the existence in HP limpets of a preparatory strategy in cellular stress response against thermal stress.Our results dem on strate that the predictability of operative temperature can affect physiological responses and population dynamics.The importance of predictability should be considered in analyses of the ecological consequences of climate warming.
文摘以钥孔戚血蓝蛋白(Keyhole limpet hemocyanin,KLH)为抗原、Montanide ISA 763 A为佐剂开展金鱼免疫试验,检测血清抗体水平,评估金鱼体液免疫应答情况,以期为其免疫策略的制定提供依据。试验在水温15~18℃时进行,采用(20±3)g体重的健康鱼150尾,随机均分为三组,第一组使用(KLH+Montanide ISA 763 A)免疫,第二组仅使用KLH免疫,第三组注射等量的0.01 mol/L磷酸盐缓冲液(Phosphate buffered solution,PBS)为对照,历经三次免疫并对4次采样的血清样本进行ELISA检测。结果显示,第一组和第二组均检测到较高的抗KLH抗体水平,在第二次、第三次免疫后,添加ISA佐剂的第一组抗体仍维持较高水平,而在仅用KLH免疫的第二组抗体水平下降,并降低到对照组水平,表明使用(KLH+Montanide ISA 763 A)免疫金鱼可以获得更加持久和稳定的免疫效果,而仅注射KLH的免疫效果较为短暂。研究还说明金鱼在低温情况下,可以产生良好的体液免疫应答。
