To obtain the wound dressings which can accelerate healing effectively,vitamin E D-α-Tocopherol polyethylene glycol succinate(vitamin E TPGS),one of the common derivatives of the unstable vitamins E,was successfully ...To obtain the wound dressings which can accelerate healing effectively,vitamin E D-α-Tocopherol polyethylene glycol succinate(vitamin E TPGS),one of the common derivatives of the unstable vitamins E,was successfully incorporated into P(LLA-CL)nanofibers by electrospinning.Electron microscopy showed that the smooth cylindrical fibers were obtained,albeit with a small amount of beading visible for the vitamins-loaded fibers.The diameters of the P(LLA-CL)fibers decreased with the addition of vitamins.The incorporation of the vitamin E TPGS in the electrospun fibers was confirmed by Fourier transform infrared spectroscopy(FTIR).Moreover,X-ray diffraction(XRD)indicated that vitamin E TPGS existed in the amorphous physical form after electrospinning.Fibers containing vitamin E TPGS showed a sustained release profile over more than 100 h in vitro.Antibacterial tests demonstrated that fibers loaded with vitamin E TPGS were effective in inhibiting the growth of E.coli and S.aureus.MTT assay showed that the fibers could promote the proliferation of L929 fibroblasts.These results above demonstrate the potential of P(LLA-CL)/vitamins E TPGS(P/E)as advanced wound dressing materials.展开更多
Curcumin exhibited excellent properties including antioxidant, anti- inflammatory, antiviral, antibacterial, antifungal, anticancer, and anticoagulant activities. In this study, curcumin was incorporated into silk fib...Curcumin exhibited excellent properties including antioxidant, anti- inflammatory, antiviral, antibacterial, antifungal, anticancer, and anticoagulant activities. In this study, curcumin was incorporated into silk fibroin （SF）/poly（L-lactic acid-co-e- caprolactone） （P（LLA-CL）） nanofibrous scaffolds via electrospinning, and changes brought about by raising the curcumin content were observed： SEM images showed that the average nanofibrous diameter decreased at the beginning and then increased, and the nanofibers became uniform; FTIR showed that the conformation of SF transforming from random coil form to β-sheet structure had not been induced, while SF conformation converted to β-sheet after being treated with 75% ethanol vapor; XRD results confirmed that the crystal structure of （P（LLA-CL）） had been destroyed; The mechanical test illustrated that nanofibrous scaffolds still maintained good mechanical properties. Further, curcumin-loaded nanofibrous scaffolds were evaluated for drug release, antioxidant and antimicrobial activities in vitro. The results showed that curcumin presented a sustained release behavior from nanofibrous scaffolds and maintained its free radical scavenging ability, and such scaffolds could effectively inhibit S. aureus growth （〉 95%）. Thus, curcumin-loaded SF/P（LLA-CL） nanofibrous scaffolds might be potential candidates for wound dressing and tissue engineering scaffolds.展开更多
Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P（LLA-CL） nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the mat...Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P（LLA-CL） nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the material as tissue engineering nerve scaffold was investigated in vitro. The average diameter increased with decreasing the blend ratio of ApF to P（LLA-CL）. Characterization of 13C NMR and FTIR clarified that there is no obvious chemical bond reaction between ApF and P（LLA-CL）. The tensile strength and elongation at break increased with the content increase of P（LLA-CL）. The surface hydrophilic property of nanofibrous scaffolds enhanced with the increased content of ApF. Cell viability studies with Schwann cells demonstrated that ApFIP（LLA-CL） blended nanofibrous scaffolds significantly promoted cell growth as compare to P（LLA-CL）, especially when the weight ratio of ApF to P（LLA-CL） was 25：75. The present work provides a basis for further studies of this novel nanofibrous material （ApF/P（LLA-CL）） in peripheral nerve tissue repair or regeneration.展开更多
A multi-layered composite scaffolds consisting of poly （ L- ne） （ P （LLA-CL） ）, collagen （COL） and chitosan （CS） were fabricated by a bi-directional electrospinnlng method. Synthetic P （LLA-CL） was used as...A multi-layered composite scaffolds consisting of poly （ L- ne） （ P （LLA-CL） ）, collagen （COL） and chitosan （CS） were fabricated by a bi-directional electrospinnlng method. Synthetic P （LLA-CL） was used as the middle layer to enhance the strength, while natural COL/CS blending （9： 1, v/v） was used as the bioactive surfaces （inner and outer layers ） to improve the biocompatibility. Each three transitional layers were set between inner/outer layer and middle layer for delamination resistance. Scanning electron microscopy （SEM） was used to observe the fiber morphology. The Fourier transform infrared attenuated total reflectance spectroscopy （FTIR-ATR） spectra, X- ray diffraction （XRD） and thermogravimetry （TG） tests were used to analyze the physical properties of the scaffolds. The results showed that the modified clectrospinning method bad no negative effect on the components, crystal structure and thermostability of the scaffolds, but could effectively combine the mechanical property of synthetic material and biocompatibility of natural materials. Such method could be applied to the fabrication of composite scaffolds for vascular, skin. and nerve tissue engineering.展开更多
Nowadays, muifichannel nerve guidance conduit （NGC） was designed by mimicking the architecture of nerve fascicles, and it was used to reduce dispersion of regenerating axons within the NGC lumen. In this paper, gela...Nowadays, muifichannel nerve guidance conduit （NGC） was designed by mimicking the architecture of nerve fascicles, and it was used to reduce dispersion of regenerating axons within the NGC lumen. In this paper, gelatin was used to prepare multichannel inner layer of NGC by freeze-drying, and poly （ L-lactic add-co-ε- caprolactone） （P（LLA-CL）） was used to fabricate nanofiber outer layer of NGC by electrospinning. The morphology of dual-layer mtlltichannel NGC was observed by scanning electron microscopy （SEM）. In vitro degradation experiment of the NGC demonstrated that the inner layer of NGC had the faster degradation rate than the outer layer of NGC. tell viability assay indicated that Schwann cells （SCs） showed better proliferation on dual-layer multichannel NGC than hollow NGC, because the multichannel structure introduced contact guidance for direct cell migration. Therefore, it was suggested that the dual-layer multichannel NGC had the potential for nerve lissue regeneration.展开更多
Recent years, it has attracted more attentions to increase the porosity and pore size of nanofibrous scaffolds to provide the for the cells to grow into the small-diameter vascular grafts. In this study, a novel bi-la...Recent years, it has attracted more attentions to increase the porosity and pore size of nanofibrous scaffolds to provide the for the cells to grow into the small-diameter vascular grafts. In this study, a novel bi-layer tubular scaffold with an inner layer and an outer layer was fabricated. The inner layer was random collagen/poly （ L-lactide-co-caprolactone ） I P （ LLA- CL） ] nanofibrous mat fabricated by conventional electrospinning and the outer layer was aligned collagen/P （LLA-CL） nanoyarns prepared by a dynamic liquid dectrospinning method. Fourier transform infrared spectroscopy （FTIR） was used to characterize the chemical structure. Scanning electron microscopy （ SEM ） was employed to observe the morphology of the layers and the cross- sectioned bi-layer tubular scaffold. A liquid displacement method was employed to measure the porosities of the inner and outer layers. Stress-strain curves were obtained to evaluate the mechanical properties of the two different layers and the bi-layer membrane. The diameters of the nanofibers and the nanoyarns were （480 ± 197 ） nm and （ 19.66 ± 4.05 ） μm, respectively. The outer layer had a significantly higher porosity and a larger pore size than those of the inner layer. Furthermore, the bi-layer membrane showed a good mechanical property which was suitable as small-diameter vascular graft. The results indicated that the bi-layer tubular scaffold had a great potential application in small vascular tissue engineering.展开更多
基金Science and Technology Commission of Shanghai Municipality,China(No.16410723700)“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)UK-China Joint Laboratory for Therapeutic Textiles Based at Donghua University
文摘To obtain the wound dressings which can accelerate healing effectively,vitamin E D-α-Tocopherol polyethylene glycol succinate(vitamin E TPGS),one of the common derivatives of the unstable vitamins E,was successfully incorporated into P(LLA-CL)nanofibers by electrospinning.Electron microscopy showed that the smooth cylindrical fibers were obtained,albeit with a small amount of beading visible for the vitamins-loaded fibers.The diameters of the P(LLA-CL)fibers decreased with the addition of vitamins.The incorporation of the vitamin E TPGS in the electrospun fibers was confirmed by Fourier transform infrared spectroscopy(FTIR).Moreover,X-ray diffraction(XRD)indicated that vitamin E TPGS existed in the amorphous physical form after electrospinning.Fibers containing vitamin E TPGS showed a sustained release profile over more than 100 h in vitro.Antibacterial tests demonstrated that fibers loaded with vitamin E TPGS were effective in inhibiting the growth of E.coli and S.aureus.MTT assay showed that the fibers could promote the proliferation of L929 fibroblasts.These results above demonstrate the potential of P(LLA-CL)/vitamins E TPGS(P/E)as advanced wound dressing materials.
基金This research was supported by the Independent Design Project of Key Scientific and Technological Innovation Team ofZhejiang Province （Grant No. 2010R50012-19）, the Key SRT Project of Jiaxing University （Grant No. 851713022）, the National Natural Science Foundation of China （Grant No. 31271035）, and Zhejiang Province Public Technology Applied Research Projects （Grant No. 2014C33005）.
文摘Curcumin exhibited excellent properties including antioxidant, anti- inflammatory, antiviral, antibacterial, antifungal, anticancer, and anticoagulant activities. In this study, curcumin was incorporated into silk fibroin （SF）/poly（L-lactic acid-co-e- caprolactone） （P（LLA-CL）） nanofibrous scaffolds via electrospinning, and changes brought about by raising the curcumin content were observed： SEM images showed that the average nanofibrous diameter decreased at the beginning and then increased, and the nanofibers became uniform; FTIR showed that the conformation of SF transforming from random coil form to β-sheet structure had not been induced, while SF conformation converted to β-sheet after being treated with 75% ethanol vapor; XRD results confirmed that the crystal structure of （P（LLA-CL）） had been destroyed; The mechanical test illustrated that nanofibrous scaffolds still maintained good mechanical properties. Further, curcumin-loaded nanofibrous scaffolds were evaluated for drug release, antioxidant and antimicrobial activities in vitro. The results showed that curcumin presented a sustained release behavior from nanofibrous scaffolds and maintained its free radical scavenging ability, and such scaffolds could effectively inhibit S. aureus growth （〉 95%）. Thus, curcumin-loaded SF/P（LLA-CL） nanofibrous scaffolds might be potential candidates for wound dressing and tissue engineering scaffolds.
基金This research was supported by the National Key Research Program of China （2016YFA0201702 of 2016YFA0201700）, the National Natural Science Foundation of China （Grant Nos. 31470941 and 31271035）, the Science and Technology Commission of Shanghai Municipality （Grant Nos. 15JC1490100 and 15441905100）, the Ph.D. Programs Foundation of Ministry of Education of China （Grant No. 20130075110005）, and the Yantai Double Hundred Talent Plan. The authors extend their appreciation to the International Scientific Partnership Program 1SPP at King Saud University for funding this research work through ISPP# 0049.
文摘Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P（LLA-CL） nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the material as tissue engineering nerve scaffold was investigated in vitro. The average diameter increased with decreasing the blend ratio of ApF to P（LLA-CL）. Characterization of 13C NMR and FTIR clarified that there is no obvious chemical bond reaction between ApF and P（LLA-CL）. The tensile strength and elongation at break increased with the content increase of P（LLA-CL）. The surface hydrophilic property of nanofibrous scaffolds enhanced with the increased content of ApF. Cell viability studies with Schwann cells demonstrated that ApFIP（LLA-CL） blended nanofibrous scaffolds significantly promoted cell growth as compare to P（LLA-CL）, especially when the weight ratio of ApF to P（LLA-CL） was 25：75. The present work provides a basis for further studies of this novel nanofibrous material （ApF/P（LLA-CL）） in peripheral nerve tissue repair or regeneration.
基金"111 Project" Biomedical Textile Materials Science and Technology,China，National Natural Science Foundations of China，Science and Technology Commission of Shanghai Municipality,China，Ph.D.Programs Foundation of Ministry of Education of China
文摘A multi-layered composite scaffolds consisting of poly （ L- ne） （ P （LLA-CL） ）, collagen （COL） and chitosan （CS） were fabricated by a bi-directional electrospinnlng method. Synthetic P （LLA-CL） was used as the middle layer to enhance the strength, while natural COL/CS blending （9： 1, v/v） was used as the bioactive surfaces （inner and outer layers ） to improve the biocompatibility. Each three transitional layers were set between inner/outer layer and middle layer for delamination resistance. Scanning electron microscopy （SEM） was used to observe the fiber morphology. The Fourier transform infrared attenuated total reflectance spectroscopy （FTIR-ATR） spectra, X- ray diffraction （XRD） and thermogravimetry （TG） tests were used to analyze the physical properties of the scaffolds. The results showed that the modified clectrospinning method bad no negative effect on the components, crystal structure and thermostability of the scaffolds, but could effectively combine the mechanical property of synthetic material and biocompatibility of natural materials. Such method could be applied to the fabrication of composite scaffolds for vascular, skin. and nerve tissue engineering.
基金National Natural Science Foundations of China(Nos.31470941,31271035)Science and Technology Commission of Shanghai Municipality,China(Nos.15JC1490100,15441905100)+2 种基金National Major Research Program of China(No.2016YFC1100200)Ph.D.Programs Foundation of Ministry of Education of China(No.20130075110005)Light of Textile Project,China(No.J201404)
文摘Nowadays, muifichannel nerve guidance conduit （NGC） was designed by mimicking the architecture of nerve fascicles, and it was used to reduce dispersion of regenerating axons within the NGC lumen. In this paper, gelatin was used to prepare multichannel inner layer of NGC by freeze-drying, and poly （ L-lactic add-co-ε- caprolactone） （P（LLA-CL）） was used to fabricate nanofiber outer layer of NGC by electrospinning. The morphology of dual-layer mtlltichannel NGC was observed by scanning electron microscopy （SEM）. In vitro degradation experiment of the NGC demonstrated that the inner layer of NGC had the faster degradation rate than the outer layer of NGC. tell viability assay indicated that Schwann cells （SCs） showed better proliferation on dual-layer multichannel NGC than hollow NGC, because the multichannel structure introduced contact guidance for direct cell migration. Therefore, it was suggested that the dual-layer multichannel NGC had the potential for nerve lissue regeneration.
基金National Natural Science Foundations of China，Science and Technology Commission of Shanghai Municipality,China，Ph.D.Programs Foundation of Ministry of Education of China
文摘Recent years, it has attracted more attentions to increase the porosity and pore size of nanofibrous scaffolds to provide the for the cells to grow into the small-diameter vascular grafts. In this study, a novel bi-layer tubular scaffold with an inner layer and an outer layer was fabricated. The inner layer was random collagen/poly （ L-lactide-co-caprolactone ） I P （ LLA- CL） ] nanofibrous mat fabricated by conventional electrospinning and the outer layer was aligned collagen/P （LLA-CL） nanoyarns prepared by a dynamic liquid dectrospinning method. Fourier transform infrared spectroscopy （FTIR） was used to characterize the chemical structure. Scanning electron microscopy （ SEM ） was employed to observe the morphology of the layers and the cross- sectioned bi-layer tubular scaffold. A liquid displacement method was employed to measure the porosities of the inner and outer layers. Stress-strain curves were obtained to evaluate the mechanical properties of the two different layers and the bi-layer membrane. The diameters of the nanofibers and the nanoyarns were （480 ± 197 ） nm and （ 19.66 ± 4.05 ） μm, respectively. The outer layer had a significantly higher porosity and a larger pore size than those of the inner layer. Furthermore, the bi-layer membrane showed a good mechanical property which was suitable as small-diameter vascular graft. The results indicated that the bi-layer tubular scaffold had a great potential application in small vascular tissue engineering.
