Several lines of evidence support the notion that increased RNA-binding ability of polypyrimidine tract-binding（PTB） proteinassociated splicing factor（PSF） and aberrant expression of long non-coding RNAs（IncRNAs...Several lines of evidence support the notion that increased RNA-binding ability of polypyrimidine tract-binding（PTB） proteinassociated splicing factor（PSF） and aberrant expression of long non-coding RNAs（IncRNAs） are associated with mouse and human tumors.To identify the PSF-binding IncRNA involved in human oncogenesis,we screened a nuclear RNA repertoire of human melanoma cell line,YUSAC,through RNA-SELEX affinity chromatography.A previously unreported IncRNA,termed as Llme23,was found to bind immobilized PSF resin.The specific binding of Llme23 to both recombinant and native PSF protein was confirmed in vitro and in vivo. The expression of PSF-binding Llme23 is exclusively detected in human melanoma lines.Knocking down Llme23 remarkably suppressed the malignant property of YUSAC cells,accompanied by the repressed expression of proto-oncogene Rab23.These results may indicate that Llme23 can function as an oncogenic RNA and directly associate the PSF-binding IncRNA with human melanoma.展开更多
基金supported by the grants from the National Natural Science Foundation of China(Nos.31000579 and 30971634)the Provincial Natural Science Foundation of Hainan of China(No.310045)+1 种基金the Ph.D.Programs Foundation of the Ministry of Education of China(No. 20090181120076)the Provincial Youth Science and Technology Innovation Team Foundation of Sichuan of China (No.2011JTD0026)
文摘Several lines of evidence support the notion that increased RNA-binding ability of polypyrimidine tract-binding（PTB） proteinassociated splicing factor（PSF） and aberrant expression of long non-coding RNAs（IncRNAs） are associated with mouse and human tumors.To identify the PSF-binding IncRNA involved in human oncogenesis,we screened a nuclear RNA repertoire of human melanoma cell line,YUSAC,through RNA-SELEX affinity chromatography.A previously unreported IncRNA,termed as Llme23,was found to bind immobilized PSF resin.The specific binding of Llme23 to both recombinant and native PSF protein was confirmed in vitro and in vivo. The expression of PSF-binding Llme23 is exclusively detected in human melanoma lines.Knocking down Llme23 remarkably suppressed the malignant property of YUSAC cells,accompanied by the repressed expression of proto-oncogene Rab23.These results may indicate that Llme23 can function as an oncogenic RNA and directly associate the PSF-binding IncRNA with human melanoma.
