Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by...Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by enzymatic alcoholysis.The MAG with 69.42%ARA at sn-2 position were obtained by solvent extraction and low temperature solvent crystallization.Secondly,the MLCT rich in ARA at sn-2 position and capric acid(CA)at sn-1,3 positions were produced by enzymatic esterification.Under the optimal conditions(MAG:CA=1:3(mol/mol),0.05 MPa vacuum,8% Lipozyme RM IM,5 h,25℃),the content of triacylglycerol was up to 93.60%.The triacylglycerol in the form of C10:0-C20:4-C10:0(including isomers)was about 40.43%.The ARA contents in the total and sn-2 fatty acid composition of the final product were 32.35%and 51.12%,respectively.MLCT rich in ARA at sn-2 position were successfully produced and the product has the potential application for functional food and infant formula.展开更多
基金supported by the National Natural Science Foundation of China(31601433)Jiangsu Province Natural Science Foundation(BK20140149).
文摘Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by enzymatic alcoholysis.The MAG with 69.42%ARA at sn-2 position were obtained by solvent extraction and low temperature solvent crystallization.Secondly,the MLCT rich in ARA at sn-2 position and capric acid(CA)at sn-1,3 positions were produced by enzymatic esterification.Under the optimal conditions(MAG:CA=1:3(mol/mol),0.05 MPa vacuum,8% Lipozyme RM IM,5 h,25℃),the content of triacylglycerol was up to 93.60%.The triacylglycerol in the form of C10:0-C20:4-C10:0(including isomers)was about 40.43%.The ARA contents in the total and sn-2 fatty acid composition of the final product were 32.35%and 51.12%,respectively.MLCT rich in ARA at sn-2 position were successfully produced and the product has the potential application for functional food and infant formula.
