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Effects of post-mortem aging process on characteristic water-soluble taste-active precursors in yellow-feathered broilers 被引量:3
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作者 Na Xu Xianming Zeng +4 位作者 Lingyun Li Xinyue Zhang Peng Wang Minyi Han Xinglian Xu 《Food Science and Human Wellness》 SCIE CSCD 2023年第1期242-253,共12页
Chilled chicken has become the mainstream of chicken consumption.In order to explore the effect of post-mortem aging on water-soluble flavor precursors of chicken,pH,adenosine triphosphate(ATP)degradation,flavor nucle... Chilled chicken has become the mainstream of chicken consumption.In order to explore the effect of post-mortem aging on water-soluble flavor precursors of chicken,pH,adenosine triphosphate(ATP)degradation,flavor nucleosides,free amino acids and water-soluble low molecular weight peptides were determined using Qingyuan partridge yellow-feathered broilers as material during 0-4℃ post-mortem aging in 48 h.The results showed that the pH value fell to the limit pH 5.64(4 h)in chicken breast and 6.21(3 h)in thigh.Regardless of chicken breast or thigh,ATP dropped rapidly within 3 h.It was found that the K-value in chicken thigh was the lowest at 2 h indicating the freshness was the best.Considering the equivalent umami concentration(EUC),the value at 3 h and 4 h was relatively high,but the corresponding electronic tongue umami value was not high,which further showed that the water-soluble low molecular taste peptide played an important role on the post-mortem aging process.Combined with cluster analysis and partial least squares discriminant analysis(PLS-DA),it was preliminarily inferred that the optimal time for chilled chicken during 0-4℃ post-mortem aging was 2 h,which could provide a theoretical basis for the further processing of fresh chicken. 展开更多
关键词 CHICKEN POST-MORTEM Flavor precursors Low molecular weight peptides(lmwps)
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肾脏疾病检测的现状与应用 被引量:8
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作者 李晓娟 张润玲 尤崇革 《微生物学免疫学进展》 2003年第2期91-94,共4页
肾脏疾病是临床上常见而又十分严重的疾病 ,若不能及时发现 ,常引起肾衰 ,危及生命。随着对肾脏病的研究 ,肾脏疾病的检测方法也有了很大进步 ,建立肾损伤早期检测体系成为当务之急 ,目前这一体系主要包括肾小球早期损伤标志物 :尿微量... 肾脏疾病是临床上常见而又十分严重的疾病 ,若不能及时发现 ,常引起肾衰 ,危及生命。随着对肾脏病的研究 ,肾脏疾病的检测方法也有了很大进步 ,建立肾损伤早期检测体系成为当务之急 ,目前这一体系主要包括肾小球早期损伤标志物 :尿微量白蛋白 (mAlb)和尿转铁蛋白 (Tf) ;肾小管早期损伤标记物 :肾小管上皮细胞的各种酶 (统称尿酶 )、各段肾小管脱落的抗原性物质和低分子量蛋白 (LMWP) ;肾小球滤过率的标志物 :胱氨酸蛋白酶抑制剂C(CystatinC)。 展开更多
关键词 肾损伤早期检测体系 MALB TF 尿酶 lmwp CYSTATIN C
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小儿Dent病临床特征和基因突变分析 被引量:10
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作者 李鹏 黄建萍 《临床儿科杂志》 CAS CSCD 北大核心 2008年第4期298-301,共4页
目的探讨小儿Dent病的临床特征和分子遗传学特点。方法以2例拟诊的Dent病患儿及其家系中的女性为研究对象。盐析法从外周血白细胞提取基因组DNA,PCR扩增CLCN5基因所有的编码序列,PCR产物直接测序。结果2例患儿均有小分子量蛋白尿和高钙... 目的探讨小儿Dent病的临床特征和分子遗传学特点。方法以2例拟诊的Dent病患儿及其家系中的女性为研究对象。盐析法从外周血白细胞提取基因组DNA,PCR扩增CLCN5基因所有的编码序列,PCR产物直接测序。结果2例患儿均有小分子量蛋白尿和高钙尿症,其中1例有肾脏钙化,2例肾功能均正常。在这2个家系发现了2个CLCN5基因的突变,分别为L594fsX595和R637X,其中L594fsX595为新发现的突变。结论持续性小分子量蛋白尿和高钙尿症是国内小儿Dent病的主要临床特征,并证实了我国Dent病患儿存在CLCN5基因的突变。 展开更多
关键词 Dent病 CLCN5 小分子量蛋白尿 高钙尿症
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