A metropolitan city such as Los Angeles (LA) is an ideal study site with a very high population density, and it houses at least 3 treatment plants where sewage is treated preliminarily and then progressing to tertiary...A metropolitan city such as Los Angeles (LA) is an ideal study site with a very high population density, and it houses at least 3 treatment plants where sewage is treated preliminarily and then progressing to tertiary treatment before discharging into the LA River. We will gain a better understanding of the water quality in the LA River and the nitrate load in the watershed system by examining the influence of waste water treatment plants (WWTPs). The goal of this study is to pinpoint the exact source of nitrate in the LA River using the isotope signatures. We have selected sampling locations both upstream and downstream of the WWTP. This serves to monitor nitrate levels, aiding in the assessment of treatment plant effectiveness, pinpointing nitrate pollution sources, and ensuring compliance with environmental regulations. The research explores the isotopic composition of NO3 in relation to atmospheric nitrogen and Vienna Standard Mean Ocean Water, shedding light on the contributions from various sources such as manure, sewage, soil organic nitrogen, and nitrogen fertilizers. Specifically, there is a change in the δ15NAir value between the dry and wet seasons. The isotope values in the Tillman WWTP sample changed between dry and wet seasons. Notably, the presence of nitrate originating from manure and sewage is consistent across seasons, emphasizing the significant impact of anthropogenic and agricultural activities on water quality. This investigation contributes to the broader understanding of nitrogen cycling in urban water bodies, particularly in the context of wastewater effluent discharge. The findings hold implications for water quality management and highlight the need for targeted interventions to mitigate the impact of nitrogen-containing compounds on aquatic ecosystems. Overall, the study provides a valuable framework for future research and environmental stewardship efforts aimed at preserving the health and sustainability of urban water resources. This data informs decisions regarding additional treatment or mitigation actions to safeguard downstream water quality and ecosystem health.展开更多
Length-weight relationships and condition factors are among the commonly used parameters to link the length of a fish to its weight. The present study was initiated to find out the growth patterns and overweight statu...Length-weight relationships and condition factors are among the commonly used parameters to link the length of a fish to its weight. The present study was initiated to find out the growth patterns and overweight status of 15 fish species in the Loémé basin in Mayombe, Republic of Congo. In the period from February 2020 to September 2021, sampling was carried out in eight stations, following a standardised capture method using four gillnets of 10, 12, 15, and 25 mm mesh size and a hawk net of 1.90 m radius with 10 mm mesh size. Length-weight relationships were calculated according to P = aLS<sup>b</sup> and the condition factor, using K = (P/LS<sup>b</sup>)* 100. A total of 1153 specimens representing 15 species, 10 genera and 6 families were recorded. The number of specimens ranged from 10 to 214 for Chrysichthys auratus and Enteromius camptacanthus, respectively. The allometry coefficient b minimum of 2.697 for Chromidotilapiamelaniae and maximum of 3.589 for Garraornata, with a mean of 3.03 ± 0.2 is significantly not different from 3 at the 0.05 probability threshold and indicates isometric growth for almost all species, except for E. camptacanthus where b = 2.858 was statistically different from 3 at the same threshold. The positive and high values of the coefficients of determination r<sup>2</sup> = 0.787-0.989 of the species, obtained during this study, suggest that weight increases with size in the fish of the Loémé basin. The average condition factor varies from 0.524 ± 0.064 for G. ornata to 4.917 ± 0.440 for C. melaniae.展开更多
Background and Objective: Despite the costs generated in the diagnosis of cardiac pathologies by the use of ultrasound, the ECG indices have demonstrated a high performance in the studies of developed countries in the...Background and Objective: Despite the costs generated in the diagnosis of cardiac pathologies by the use of ultrasound, the ECG indices have demonstrated a high performance in the studies of developed countries in the diagnosis of these pathologies, but the data of these in sub-Saharan Africa are limited. The objective of this study is to evaluate the performance of the Peguero-Lo Presti index in the diagnosis of LVH among Congolese in order to make it a means of LVH diagnosis in an under-equipped environment. Methods: Cross-sectional and analytical study including 413 patients followed and hospitalized at the CIMAK Hospital Center during the period from February 2019 to June 2021. Sociodemographic parameters, history, biology, ultrasound and ECG parameters were studied. The performance of the Peguero-Lo Presti Index was validated by the Youden Index reinforced by the Area under the ROC curve. Results: Of the 413 patients admitted to the study, 69.5% were men versus 30.5% women, i.e. a sex ratio of 2M/1F;the mean age of the patients was 51.1 ± 11.6 years. The frequency of LVH found by echocardiography was 55.9%, it was 50.8% using the Peguero-Lo Presti index, 22% and 10.2% using the Peguero-Lo Presti index, respectively. Sokolow-Lyon and Cornell (p 0.001). The Peguero-Lo Presti curve is higher than the Sokolow-Lyon and Cornell curves. The area under the curve was 0.80 for Peguero-Lo Presti, 0.73 for Sokolow-Lyon and 0.66 for cornell. Conclusion: The newly proposed Peguero-Lo Presti index provides high sensitivity and specificity for the diagnosis of LVH in black Africans as also reported in the European and American population.展开更多
针对超宽带(ultra wide band,UWB)定位中影响定位精度的非视距(non line of sight,NLoS)传播误差问题,提出了一种基于Kalman滤波的NLoS误差二次消除方法.该方法利用NLoS误差与测量误差之间的相互独立性,借助Kalman滤波将NLoS误差从总误...针对超宽带(ultra wide band,UWB)定位中影响定位精度的非视距(non line of sight,NLoS)传播误差问题,提出了一种基于Kalman滤波的NLoS误差二次消除方法.该方法利用NLoS误差与测量误差之间的相互独立性,借助Kalman滤波将NLoS误差从总误差中单独分离出来,对其进行实时估计,并将该NLoS误差估计值作为NLoS误差辨别及测距值修正的依据.通过Kalman滤波对到达时间(time of arrival,TOA)测距值进行二次估计、鉴别及修正以提高TOA测距精度,从而实现室内复杂环境下的UWB精准实时定位.仿真实验结果表明:该方法不仅能够对NLoS误差实现良好的跟踪估计,对视距(line of sight,LoS)/NLoS环境转变也具有较强的灵敏感知能力,同时NLoS误差测距值在应用该方法后的定位性能逼近于LoS环境下的理想状态.展开更多
西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制...西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制率、谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)泄漏量越大,相同乙醇浓度下,红西凤和食用酒精(对照组)干预组之间具有显著性差异(P<0.05)。动物实验结果表明,不同剂量红西凤和食用酒精干预均导致小鼠体重、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)含量降低,肝重体重比、AST、ALT、TC、总胆固醇(total cholesterol,TC)、总甘油三酯(total triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)含量升高,肝脏组织出现不同程度脂肪变性,肠道菌群发生明显变化(门水平上,厚壁菌门与拟杆菌门比例下降;属水平上,乳酸杆菌属、毛螺菌属等相对丰度降低,幽门螺杆菌属、链球菌属等相对丰度增加),LEfSe分析结果显示,小鼠肠道菌群存在的差异与红西凤和食用酒精干预剂量有一定关系,且高剂量干预对小鼠肠道菌群的影响有扩大的趋势,然而同剂量下,红西凤干预对小鼠的损伤程度轻于食用酒精,以低剂量红西凤干预对小鼠的影响最轻,推测红西凤中各微量成分可能通过调节小鼠肠道菌群从而减缓对肝脏的损伤程度。以上结果说明,适量摄入红西凤在一定程度上可以缓解酒精对LO2细胞及小鼠肠道菌群的负面影响,该研究将为西凤酒的健康消费提供理论依据。展开更多
Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocreba...Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocrebanine on microtubule network homeostasis at both molecular and cellular levels.Methods:the EBI site competition method and molecular docking method were used to determine the occupation of the microtubule site of oxocrebanine.Western Blot was used to detect the effect of oxocrebanine on microtubule-associated proteins including STAT3,PAK1,CAMK4,and PKA.Results:The results of EBI site competition assay showed that the binding of EBI toβ-Tubulin covalent fusions produced adducts that appeared in regions of lower molecular weight thanβ-tubulin(ctrl 2).Molecular docking results showed that oxocrebanine could occupy the colchicine site of microtubule proteins.As revealed by Western Blot,the expression of STAT3 protein was decreased after MCF-7 cells have been treated with low,medium,and high concentration of oxocrebanine or the positive drug taxol for 48 h(P<0.01).The expression levels of PAK1 and Camk4 proteins aslo showed significant reductions(P<0.05,or P<0.01).Oxocrebanine also decreased the PKA protein in MCF-7 cells compared to the control group(P<0.01).Conclusions:Oxocrebanine,a ligand that binds at the colchicine site of tubulin,perturbs tubulin polymerization and causes mitosis in MCF-7 cells,thus leading to MCF-7 cell death.Oxocrebanine may promote microtubule dynamics through stathmin by inhibiting the expression levels of STAT3,PAK1,Camk4,and PKA proteins in MCF-7 cells.Oxocrebanine interfers with spindle formation,and ultimately causes mitotic catastrophe in MCF-7 cells.展开更多
文摘A metropolitan city such as Los Angeles (LA) is an ideal study site with a very high population density, and it houses at least 3 treatment plants where sewage is treated preliminarily and then progressing to tertiary treatment before discharging into the LA River. We will gain a better understanding of the water quality in the LA River and the nitrate load in the watershed system by examining the influence of waste water treatment plants (WWTPs). The goal of this study is to pinpoint the exact source of nitrate in the LA River using the isotope signatures. We have selected sampling locations both upstream and downstream of the WWTP. This serves to monitor nitrate levels, aiding in the assessment of treatment plant effectiveness, pinpointing nitrate pollution sources, and ensuring compliance with environmental regulations. The research explores the isotopic composition of NO3 in relation to atmospheric nitrogen and Vienna Standard Mean Ocean Water, shedding light on the contributions from various sources such as manure, sewage, soil organic nitrogen, and nitrogen fertilizers. Specifically, there is a change in the δ15NAir value between the dry and wet seasons. The isotope values in the Tillman WWTP sample changed between dry and wet seasons. Notably, the presence of nitrate originating from manure and sewage is consistent across seasons, emphasizing the significant impact of anthropogenic and agricultural activities on water quality. This investigation contributes to the broader understanding of nitrogen cycling in urban water bodies, particularly in the context of wastewater effluent discharge. The findings hold implications for water quality management and highlight the need for targeted interventions to mitigate the impact of nitrogen-containing compounds on aquatic ecosystems. Overall, the study provides a valuable framework for future research and environmental stewardship efforts aimed at preserving the health and sustainability of urban water resources. This data informs decisions regarding additional treatment or mitigation actions to safeguard downstream water quality and ecosystem health.
文摘Length-weight relationships and condition factors are among the commonly used parameters to link the length of a fish to its weight. The present study was initiated to find out the growth patterns and overweight status of 15 fish species in the Loémé basin in Mayombe, Republic of Congo. In the period from February 2020 to September 2021, sampling was carried out in eight stations, following a standardised capture method using four gillnets of 10, 12, 15, and 25 mm mesh size and a hawk net of 1.90 m radius with 10 mm mesh size. Length-weight relationships were calculated according to P = aLS<sup>b</sup> and the condition factor, using K = (P/LS<sup>b</sup>)* 100. A total of 1153 specimens representing 15 species, 10 genera and 6 families were recorded. The number of specimens ranged from 10 to 214 for Chrysichthys auratus and Enteromius camptacanthus, respectively. The allometry coefficient b minimum of 2.697 for Chromidotilapiamelaniae and maximum of 3.589 for Garraornata, with a mean of 3.03 ± 0.2 is significantly not different from 3 at the 0.05 probability threshold and indicates isometric growth for almost all species, except for E. camptacanthus where b = 2.858 was statistically different from 3 at the same threshold. The positive and high values of the coefficients of determination r<sup>2</sup> = 0.787-0.989 of the species, obtained during this study, suggest that weight increases with size in the fish of the Loémé basin. The average condition factor varies from 0.524 ± 0.064 for G. ornata to 4.917 ± 0.440 for C. melaniae.
文摘Background and Objective: Despite the costs generated in the diagnosis of cardiac pathologies by the use of ultrasound, the ECG indices have demonstrated a high performance in the studies of developed countries in the diagnosis of these pathologies, but the data of these in sub-Saharan Africa are limited. The objective of this study is to evaluate the performance of the Peguero-Lo Presti index in the diagnosis of LVH among Congolese in order to make it a means of LVH diagnosis in an under-equipped environment. Methods: Cross-sectional and analytical study including 413 patients followed and hospitalized at the CIMAK Hospital Center during the period from February 2019 to June 2021. Sociodemographic parameters, history, biology, ultrasound and ECG parameters were studied. The performance of the Peguero-Lo Presti Index was validated by the Youden Index reinforced by the Area under the ROC curve. Results: Of the 413 patients admitted to the study, 69.5% were men versus 30.5% women, i.e. a sex ratio of 2M/1F;the mean age of the patients was 51.1 ± 11.6 years. The frequency of LVH found by echocardiography was 55.9%, it was 50.8% using the Peguero-Lo Presti index, 22% and 10.2% using the Peguero-Lo Presti index, respectively. Sokolow-Lyon and Cornell (p 0.001). The Peguero-Lo Presti curve is higher than the Sokolow-Lyon and Cornell curves. The area under the curve was 0.80 for Peguero-Lo Presti, 0.73 for Sokolow-Lyon and 0.66 for cornell. Conclusion: The newly proposed Peguero-Lo Presti index provides high sensitivity and specificity for the diagnosis of LVH in black Africans as also reported in the European and American population.
文摘针对超宽带(ultra wide band,UWB)定位中影响定位精度的非视距(non line of sight,NLoS)传播误差问题,提出了一种基于Kalman滤波的NLoS误差二次消除方法.该方法利用NLoS误差与测量误差之间的相互独立性,借助Kalman滤波将NLoS误差从总误差中单独分离出来,对其进行实时估计,并将该NLoS误差估计值作为NLoS误差辨别及测距值修正的依据.通过Kalman滤波对到达时间(time of arrival,TOA)测距值进行二次估计、鉴别及修正以提高TOA测距精度,从而实现室内复杂环境下的UWB精准实时定位.仿真实验结果表明:该方法不仅能够对NLoS误差实现良好的跟踪估计,对视距(line of sight,LoS)/NLoS环境转变也具有较强的灵敏感知能力,同时NLoS误差测距值在应用该方法后的定位性能逼近于LoS环境下的理想状态.
文摘西凤酒中微量成分种类丰富,但关于其微量成分的作用及量效关系研究较少。因此,该研究基于细胞和动物实验探讨了西凤酒(以红西凤为代表)中微量成分对LO2细胞和健康小鼠肠道菌群的影响情况。细胞实验结果表明,乙醇浓度越高,细胞增殖抑制率、谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)泄漏量越大,相同乙醇浓度下,红西凤和食用酒精(对照组)干预组之间具有显著性差异(P<0.05)。动物实验结果表明,不同剂量红西凤和食用酒精干预均导致小鼠体重、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)含量降低,肝重体重比、AST、ALT、TC、总胆固醇(total cholesterol,TC)、总甘油三酯(total triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)含量升高,肝脏组织出现不同程度脂肪变性,肠道菌群发生明显变化(门水平上,厚壁菌门与拟杆菌门比例下降;属水平上,乳酸杆菌属、毛螺菌属等相对丰度降低,幽门螺杆菌属、链球菌属等相对丰度增加),LEfSe分析结果显示,小鼠肠道菌群存在的差异与红西凤和食用酒精干预剂量有一定关系,且高剂量干预对小鼠肠道菌群的影响有扩大的趋势,然而同剂量下,红西凤干预对小鼠的损伤程度轻于食用酒精,以低剂量红西凤干预对小鼠的影响最轻,推测红西凤中各微量成分可能通过调节小鼠肠道菌群从而减缓对肝脏的损伤程度。以上结果说明,适量摄入红西凤在一定程度上可以缓解酒精对LO2细胞及小鼠肠道菌群的负面影响,该研究将为西凤酒的健康消费提供理论依据。
基金Natural Science Foundation of Hainan Province(No.820RC776)。
文摘Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocrebanine on microtubule network homeostasis at both molecular and cellular levels.Methods:the EBI site competition method and molecular docking method were used to determine the occupation of the microtubule site of oxocrebanine.Western Blot was used to detect the effect of oxocrebanine on microtubule-associated proteins including STAT3,PAK1,CAMK4,and PKA.Results:The results of EBI site competition assay showed that the binding of EBI toβ-Tubulin covalent fusions produced adducts that appeared in regions of lower molecular weight thanβ-tubulin(ctrl 2).Molecular docking results showed that oxocrebanine could occupy the colchicine site of microtubule proteins.As revealed by Western Blot,the expression of STAT3 protein was decreased after MCF-7 cells have been treated with low,medium,and high concentration of oxocrebanine or the positive drug taxol for 48 h(P<0.01).The expression levels of PAK1 and Camk4 proteins aslo showed significant reductions(P<0.05,or P<0.01).Oxocrebanine also decreased the PKA protein in MCF-7 cells compared to the control group(P<0.01).Conclusions:Oxocrebanine,a ligand that binds at the colchicine site of tubulin,perturbs tubulin polymerization and causes mitosis in MCF-7 cells,thus leading to MCF-7 cell death.Oxocrebanine may promote microtubule dynamics through stathmin by inhibiting the expression levels of STAT3,PAK1,Camk4,and PKA proteins in MCF-7 cells.Oxocrebanine interfers with spindle formation,and ultimately causes mitotic catastrophe in MCF-7 cells.
