Background:Renalfibrosis is an important process in the development of chronic kidney disease.Understanding the pathogenesis andfinding effective treatments for renalfibrosis is crucial.This study aims to investigate whe...Background:Renalfibrosis is an important process in the development of chronic kidney disease.Understanding the pathogenesis andfinding effective treatments for renalfibrosis is crucial.This study aims to investigate whether a newly discovered long non-coding RNA(lncRNA)called LOC103694972 could be a potential target for treatingfibrosis of NRK-49F cells.Methods:LncRNA Chip was used to identify differentially expressed lncRNAs between TGF-β1-induced NRK-49F cells and normal cells.The dual-luciferase assay confirmed the binding between miR-29c-3p and signal transducer and activator of transcription(STAT3),as well as between miR-29c-3p and lncRNA LOC103694972.Si-LOC103694972 and miR-29c-3p mimic were then transfected into TGF-β1-induced NRK-49F cells.Results:The study found that LOC103694972 was highly expressed in TGF-β1-induced NRK-49F cells.These cells exhibited increased cell length and activity compared to the control group.The expression levels of Collagen I,α-Smooth muscle actin(α-SMA),and tissue inhibitor of metalloproteinase(TIMP-1)were increased,while matrix Metalloproteinase 2(MMP2)and matrix Metalloproteinase 9(MMP9)expression was decreased.However,transfection with si-LOC103694972 and miR-29c-3p mimics restored cell morphology and reduced cell viability.This led to a decrease in the levels of Collagen I,α-SMA,and TIMP-1,as well as an increase in MMP2 and MMP9 expression.Additionally,TGF-β1-induced NRK-49F cells transfected with miR-29c-3p mimics activated the STAT3-Smad3/CTGF pathway.Conclusion:Based on thesefindings,lncRNA LOC103694972 shows promise as a target for treating renalfibrosis.It negatively regulates miR-29c-3p and activates the STAT3-Smad3/CTGF pathway.展开更多
目的探讨胆总管结石患者行腹腔镜胆总管探查、一期缝合、同期逆行鼻胆管引流的应用价值。方法选取2014年9月—2022年6月南京医科大学附属泰州人民医院肝胆胰外科行腹腔镜下胆总管切开取石、一期缝合、同期逆行鼻胆管引流术的胆总管结石...目的探讨胆总管结石患者行腹腔镜胆总管探查、一期缝合、同期逆行鼻胆管引流的应用价值。方法选取2014年9月—2022年6月南京医科大学附属泰州人民医院肝胆胰外科行腹腔镜下胆总管切开取石、一期缝合、同期逆行鼻胆管引流术的胆总管结石患者120例(一期缝合组),另取2015年1月—2022年6月行腹腔镜下胆总管切开取石、腹腔镜胆道探查术(laparoscopic common bile duct exploration,LCBDE)的胆总管结石患者130例(LCBDE组),及2015年1月—2022年6月行内窥镜下胆管逆行造影、oddi括约肌切开取石(endoscopic sphincterotomy,EST)、同期经腹腔镜行胆囊切除术的胆总管结石患者100例(EST组),比较一期缝合组与其他两组患者的手术时间、术中出血量、近期并发症、术后住院时间和住院费用等指标,并进行术后6个月以上的随访。结果一期缝合组较LCBDE组在腹腔引流管放置时间、术后住院时间及术后恢复正常生活时间上有优势(P<0.05)。一期缝合组与EST组相比,在手术时间、术后住院时间、住院费用、术后胆道感染、并发胰腺炎等方面优势明显(P<0.05)。结论胆总管结石患者行腹腔镜胆总管探查、一期缝合、同期逆行鼻胆管引流术,安全、经济,且能保留患者乳头功能,提高患者舒适性。展开更多
基金This work was supported by the Hunan Provincial Education Department General Project Research Fund(No.20C1412)the Hunan Graduate Scientific Research Innovation Project(No.CX2018B474)the National Famous Elderly Chinese Medicine Experts Xinyu Chen Inheritance Workshop Construction Project(No.[2022]75).
文摘Background:Renalfibrosis is an important process in the development of chronic kidney disease.Understanding the pathogenesis andfinding effective treatments for renalfibrosis is crucial.This study aims to investigate whether a newly discovered long non-coding RNA(lncRNA)called LOC103694972 could be a potential target for treatingfibrosis of NRK-49F cells.Methods:LncRNA Chip was used to identify differentially expressed lncRNAs between TGF-β1-induced NRK-49F cells and normal cells.The dual-luciferase assay confirmed the binding between miR-29c-3p and signal transducer and activator of transcription(STAT3),as well as between miR-29c-3p and lncRNA LOC103694972.Si-LOC103694972 and miR-29c-3p mimic were then transfected into TGF-β1-induced NRK-49F cells.Results:The study found that LOC103694972 was highly expressed in TGF-β1-induced NRK-49F cells.These cells exhibited increased cell length and activity compared to the control group.The expression levels of Collagen I,α-Smooth muscle actin(α-SMA),and tissue inhibitor of metalloproteinase(TIMP-1)were increased,while matrix Metalloproteinase 2(MMP2)and matrix Metalloproteinase 9(MMP9)expression was decreased.However,transfection with si-LOC103694972 and miR-29c-3p mimics restored cell morphology and reduced cell viability.This led to a decrease in the levels of Collagen I,α-SMA,and TIMP-1,as well as an increase in MMP2 and MMP9 expression.Additionally,TGF-β1-induced NRK-49F cells transfected with miR-29c-3p mimics activated the STAT3-Smad3/CTGF pathway.Conclusion:Based on thesefindings,lncRNA LOC103694972 shows promise as a target for treating renalfibrosis.It negatively regulates miR-29c-3p and activates the STAT3-Smad3/CTGF pathway.
文摘目的探讨胆总管结石患者行腹腔镜胆总管探查、一期缝合、同期逆行鼻胆管引流的应用价值。方法选取2014年9月—2022年6月南京医科大学附属泰州人民医院肝胆胰外科行腹腔镜下胆总管切开取石、一期缝合、同期逆行鼻胆管引流术的胆总管结石患者120例(一期缝合组),另取2015年1月—2022年6月行腹腔镜下胆总管切开取石、腹腔镜胆道探查术(laparoscopic common bile duct exploration,LCBDE)的胆总管结石患者130例(LCBDE组),及2015年1月—2022年6月行内窥镜下胆管逆行造影、oddi括约肌切开取石(endoscopic sphincterotomy,EST)、同期经腹腔镜行胆囊切除术的胆总管结石患者100例(EST组),比较一期缝合组与其他两组患者的手术时间、术中出血量、近期并发症、术后住院时间和住院费用等指标,并进行术后6个月以上的随访。结果一期缝合组较LCBDE组在腹腔引流管放置时间、术后住院时间及术后恢复正常生活时间上有优势(P<0.05)。一期缝合组与EST组相比,在手术时间、术后住院时间、住院费用、术后胆道感染、并发胰腺炎等方面优势明显(P<0.05)。结论胆总管结石患者行腹腔镜胆总管探查、一期缝合、同期逆行鼻胆管引流术,安全、经济,且能保留患者乳头功能,提高患者舒适性。
文摘目的:利用多中心样本探究长非编码RNA LOC285194在宫颈癌组织中的表达水平及相关内源竞争RNA网络。方法:收集来自GEO、TCGA、ArrayExpress、SRA等数据库中与宫颈癌相关的高通量数据集,利用散点图和独立样本t检验探究各个数据集中LOC285194在宫颈癌组织与非癌宫颈组织对照中的表达差异。同时,合并计算标准化均数差(SMD)以综合评估LOC285194在宫颈癌组织中的表达水平。DIANA-LncBase v3和TargetScan Human v8.0数据库用于预测与LOC285194有靶向关系的miRNA以及相关的mRNA,ENCORI数据库用于计算miRNA与相应靶基因表达的相关性。结果:通过综合计算618例样本(CC样本399例,正常宫颈样本219例)的SMD,结果提示LOC285194在宫颈癌组织中的表达水平显著低于正常对照组织(SMD=-0.43,95%CI-0.61~-0.25,P<0.05)。结合预测以及综合计算SMD,3个与LOC285194相关的miRNA被纳入:hsa-miR-130b-5p、hsa-miR-141-5p和hsa-miR-200a-5p。同时,通过综合计算,hsa-miR-141-5p在宫颈癌组织中的高表达及其靶基因ZNF385D的低表达得到了验证。结论:LOC285194在宫颈癌组织中呈显著的低表达,其可能通过相关的内源竞争RNA机制参与宫颈癌的发生与发展,值得进一步的研究探讨。
