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黑果枸杞LrANS基因的克隆及表达分析 被引量:3
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作者 王联星 马得森 +5 位作者 史国民 高思丹 郭佳磊 赵伟民 李凤珍 何涛 《中国细胞生物学学报》 CAS CSCD 2019年第10期1929-1937,共9页
以黑果枸杞为材料,利用同源克隆和RACE技术克隆了花青素合成相关基因LrANS(Gen Bank登录号为MK713977)。序列分析表明, LrANS基因cDNA全长为1 527 bp,包含1 290 bp的开放阅读框,编码429个氨基酸;亚细胞定位显示, LrANS主要分布于细胞质... 以黑果枸杞为材料,利用同源克隆和RACE技术克隆了花青素合成相关基因LrANS(Gen Bank登录号为MK713977)。序列分析表明, LrANS基因cDNA全长为1 527 bp,包含1 290 bp的开放阅读框,编码429个氨基酸;亚细胞定位显示, LrANS主要分布于细胞质及细胞膜上;同源序列比对表明, LrANS与烟草Nt ANS的氨基酸序列相似性较高,达到77.2%。RT-PCR分析显示, LrANS基因在根、茎、叶、花、青果、紫果和黑果中均有表达,且在黑果中的表达显著高于其他组织;在紫外胁迫下, LrANS基因的表达显著下降。推测LrANS基因在花青素合成中发挥着重要作用。 展开更多
关键词 黑果枸杞 花青素 lrans基因 紫外胁迫
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过表达LrAN2基因对马铃薯中花青素和糖苷生物碱含量的影响 被引量:2
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作者 李红艳 李洁雅 +3 位作者 李响 叶广继 周云 王舰 《作物学报》 CAS CSCD 北大核心 2023年第4期988-995,共8页
目前对马铃薯(Solanum tuberosum L.)中MYB基因参与的花青素研究较为深入,但对影响马铃薯品质和安全的糖苷生物碱(steroidal glycoalkaloids,SGAs)在不同组织中的变化规律及调控机制尚不完全清楚。LrAN2是黑果枸杞(Lycium ruthenicum)中... 目前对马铃薯(Solanum tuberosum L.)中MYB基因参与的花青素研究较为深入,但对影响马铃薯品质和安全的糖苷生物碱(steroidal glycoalkaloids,SGAs)在不同组织中的变化规律及调控机制尚不完全清楚。LrAN2是黑果枸杞(Lycium ruthenicum)中的MYB基因,与黑果枸杞果实中花青素的积累有关。本研究以野生型大西洋、2种转LrAN2大西洋株系(LrAN2oe#66和LrAN2oe#200)为试验材料,检测不同组织中的花青素和SGAs含量,并对SGAs生物合成相关基因的表达量分析。pH示差法检测花青素发现,仅在转基因植株(LrAN2oe#200)叶片中检测到一定量的花青素(12 mg 100 g-1FW)。高效液相色谱-串联质谱法检测SGAs发现,3个材料不同组织SGAs含量变化为叶片>薯皮>薯肉。薯皮中的SGAs含量无显著性差异;薯肉中LrAN2oe#66的SGAs含量较对照降低、LrAN2oe#200对照显著增加1.3倍,但未超出安全标准(0.2mg g-1FW);叶片中LrAN2oe#66的SGAs含量较对照增加1倍、LrAN2oe#200较对照显著增加3.8倍。qRT-PCR分析基因表达量发现,StHMG1和StSGT2基因受到LrAN2基因的调控在转基因植株中显著上调。本研究结果对马铃薯植株中花青素含量的积累具有指导意义,为进一步解析马铃薯资源中花青素及SGAs的调控机制提供理论依据。 展开更多
关键词 LrAN2基因 马铃薯 花青素 糖苷生物碱
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枸杞MYB转录因子LrAN2在番茄中的过量表达分析 被引量:2
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作者 罗香怡 李云 +3 位作者 曹东 魏乐 宗渊 刘宝龙 《西北农业学报》 CAS CSCD 北大核心 2021年第9期1374-1381,共8页
为明确黑枸杞LrAN2基因在花青素合成代谢中的调控机制,在与枸杞同属的番茄中做了进一步验证。黑枸杞LrAN2基因参与调控黑色果实花青素生物合成代谢,同时诱导烟草产生紫色表型,受限于枸杞转基因组培技术,选择枸杞亲缘物种番茄进行LrAN2... 为明确黑枸杞LrAN2基因在花青素合成代谢中的调控机制,在与枸杞同属的番茄中做了进一步验证。黑枸杞LrAN2基因参与调控黑色果实花青素生物合成代谢,同时诱导烟草产生紫色表型,受限于枸杞转基因组培技术,选择枸杞亲缘物种番茄进行LrAN2转基因功能分析及其调控机制。基于前期分离的LrAN2开放阅读框序列,通过表达载体构建,荧光定量分析,花青素含量的测定以及液相色谱分析方式对转基因番茄进行检测和分析。结果表明,在番茄材料Maker中过量表达LrAN2基因,得到深绿色番茄植株。转基因番茄的各个组织部位均有不同程度的花青素积累,花、叶和根细胞中都存在颜色较深色素沉着,叶片中花青素含量和LrAN2基因的表达量都是最高。LrAN2的过表达上调DFR和F3′5′H的表达水平,同时激活飞燕草色素和矢车菊色素的积累。说明LrAN2基因能够调控花青素的合成。 展开更多
关键词 番茄 花青素 LrAN2
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Geochemical characteristics of Galandrud coal in central Alborz, Iran 被引量:1
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作者 Ardebili Leila Babazadeh Vasef +1 位作者 Mammadov Mousa Navi Pedram 《International Journal of Mining Science and Technology》 2012年第1期101-106,共6页
This study focuses on the geochemistry of trace elements and parameters of coal quality of bituminous coal belonging to the upper Triassic-lower Jurassic era, collected from the Galandrud region in Central Alborz, nor... This study focuses on the geochemistry of trace elements and parameters of coal quality of bituminous coal belonging to the upper Triassic-lower Jurassic era, collected from the Galandrud region in Central Alborz, northern Iran. Galandrud coal on an air-dried basis are characterized by broad variation in the amount of ash (ranging from 6.49g to 12.73%), a high content of volatile matter (33,32-37.45%) and high gross calorific values (7430-8880 kcal/kg). The mineral matter of the coal samples studied is made up of dolomite (〉909g), quartz, sphalerite, galena, pyrite and clay minerals. Major elements are positively cor- related with ash content suggesting an association with inorganic constituents. Based on statistical anal- yses, concentrations of trace elements such as Co (51.3 ×10^-6-152.9× 10^-6), Ba (368× 10^-6-3297.4×10^-6), Cr (65.5 ×10^-6-194.2 × 10^-6), Mo (5.6 × 10^-6-18.2× 10^-6), Hi (72.9 × 10^-6-152.9 × 10^-6) and V (245.5 × 10^-6-520 ×10^-6) are higher than world coal averages. However, low concentration of some vol- atile elements such as As, Hg and U are present in low concentrations of the various types of coal studied. 展开更多
关键词 Galandrud coals GEOCHEMISTRY Trace elements Central Alborz lran
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The effect of single-tree selction system on soil properties in an oriental beech stand of Hyrcanian forest, north of Iran
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作者 Kambiz Abrari Vajari Hamid Jalilvand +2 位作者 Mohammad Reza Pourmajidian Kambiz Espahbodi Alireza Moshki 《Journal of Forestry Research》 SCIE CAS CSCD 2011年第4期591-596,共6页
A case study was conducted in beech forests of northern Iran to determine the effect of the created gaps on some soil properties in beech stand. Changes of soil properties in small (60-150 mE), medium (151-241 mE)... A case study was conducted in beech forests of northern Iran to determine the effect of the created gaps on some soil properties in beech stand. Changes of soil properties in small (60-150 mE), medium (151-241 mE), large (242-332 m^2) and very large (333-550 m^2) gaps, as well as under closed stands were studied eight years after-the gap creation. Soil samples were taken from three depths, 0-10, 10-20 and 20-30 cm. The gaps were different from their around undisturbed stands in terms of the following soil parameters: Mg^+2 concentration of 0-10 cm at medium gap size, bulk density of 10-20 cm at very large gap size as well as K^+ and Ca^+2 concentrations at 20-30 cm at small and large gap sizes, respectively. Furthermore, the size of the gaps had no effect on soil characteristics through the whole profile. Water saturation percent (Sp %) at 0-10cm as well as P and Mg^+2 at 20--30 cm was different amongst undisturbed stands around different gap sizes. The center and the edges of the gap were different only in terms of organic carbon at the depth of 10-20 cm. Significant differences were observed between gaps and closed canopy regarding P and Ca^+2 at depth 0--10 cm and 10-20 cm, respectively. It can be concluded that applied silvicultural system for harvesting trees which created these gaps might be suitable for conservation and forest management in the region. 展开更多
关键词 gap soil Fagus orientalis single-tree selection system northern lran
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Compare the model of Iran national quality award and the EFQM model
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作者 Saleh Mojdehi 《Chinese Business Review》 2010年第9期61-64,共4页
Nowadays, there are a lot of challenges for global processing in countries economic companies. One of the important reasons is lack of changes that must be with world-class technology for making a competition in indus... Nowadays, there are a lot of challenges for global processing in countries economic companies. One of the important reasons is lack of changes that must be with world-class technology for making a competition in industry, and another important reason is lack of organized distinction for managers. Most of high rank managers don't have good points for charging and worry about it. In this essay, there have been excellence organized model of EFQM and Iran national quality award which are compared by a case study, and finally, it has suggested using a native model. 展开更多
关键词 model of lran national quality award EFQM SELF-EVALUATION
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Analysis of Drilling Troubles in Kazhdumi Formation of Yadavaran Oilfield, Iran
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作者 Bo Min Xu Enxin +1 位作者 Jin Ruihuan Peng Nian 《China Oil & Gas》 CAS 2015年第2期21-25,共5页
Drilling crews of Sinopec have encountered a series of troubles when drilling a formation called kazhdumi of Yadavaran oilfield, Iran in the last few years. The formation is buried in the depth from 3300m to 3500m, ma... Drilling crews of Sinopec have encountered a series of troubles when drilling a formation called kazhdumi of Yadavaran oilfield, Iran in the last few years. The formation is buried in the depth from 3300m to 3500m, mainly including limestone, muddy limestone and some bituminous shale. Many microfissures exist in the formation and the equivalent density of formation pore pressure ranged from 1.19g/cm3 to 1.59g/cm3. Asphalt or heavy oil kicks occurred in drilling three wells in one and a half years, which contaminated the drilling fluid and made well killing difficult due to the kicks and lost circulation in open hole. Gas and hydrogen sulfide (the concentration is up to 40000ppm) were present in the wells, and finally two wells were completely abandoned and one partially losL which frustrated the drilling engineering seriously. By analyzing the data of &e drilled wells, the paper summarized the causes of the troubles, and put forward suggestions and conclusions that can serve as reference and guideline for drilling well in &is area in the future. 展开更多
关键词 lran Yadavaran Kazhdumi Formation DRILLING heavy oil hydrogen sulfide well control
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Factors Influencing the Success of Agricultural Information System (AIS): A Factor Analysis
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作者 M. Ahmadvand E. Karami 《Journal of Agricultural Science and Technology》 2011年第1期101-108,共8页
This study aims at investigating the AIS (Agricultural Information System) in Hamedan province, Iran, benefiting from the list of criteria for well functioning AISs that were identified in earlier researches. For th... This study aims at investigating the AIS (Agricultural Information System) in Hamedan province, Iran, benefiting from the list of criteria for well functioning AISs that were identified in earlier researches. For this purpose, a survey method with multi-stage stratified random sampling technique was used to select 31 agricultural researchers, and 62 extension workers in the province. The data were acquired by questionnaire and structured interviews. The instrument for data collection was subjected to pre-testing, validation and reliability tests. The results of the factor analysis revealed that the criteria could be categorized into four overarching groups based on their inter-correlation. The analysis of mentioned criteria in Hamedan province AIS showed less efficiency and effectiveness than studies conducted in other areas. But among these, two desirable criteria existed. It is recommended that the ecological knowledge system (EKS) should be integrated with AIS, and some ecological and sustainable development criteria should be added to the existing ones. 展开更多
关键词 Agricultural Information System (AIS) KNOWLEDGE EXTENSION researchers FARMERS lran
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Numerical Investigation of Flutter Stability in Subsonic Space Turbine Blisk with Emphasis on Cut-on/Cut-off Modes and Interblade Phase Angles 被引量:1
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作者 Hakim Ferria Pascal Ferrand +1 位作者 Franois Pacull Stéphane Aubert 《Journal of Thermal Science》 SCIE EI CAS CSCD 2012年第6期492-499,共8页
The so-called blisks,i.e.integrally bladed disks,are characterized by very low viscous material damping and make the flutter prediction much more critical.In that framework,a two-dimensional numerical study of a space... The so-called blisks,i.e.integrally bladed disks,are characterized by very low viscous material damping and make the flutter prediction much more critical.In that framework,a two-dimensional numerical study of a space turbine blisk featuring complex deformation of blades and high eigenfrequency(>40kHz)is performed.The simulations are based on unsteady Reynolds Averaged Navier Stokes computations linearized in the frequency domain and consist in the superposition of an unsteady linear(in time)pressure field,generated by a harmonic perturbation,upon a steady nonlinear(in space)flow.The aerodynamic damping coefficient is calculated over a range of nodal diameters,and the blades are predicted aeroelastically stable.However,violent changes occur and are rather critical since sudden and large deviations in stability appear.In that context,the nature of the waves propagating from the cascade are evaluated.Such an approach provides fundamental knowledge about the perturbations which can either propagate to the far-field(cut-on mode)or decay(cut-off mode).It is expected that the ability of the flow to damp or to amplify the blade motion is strongly affected by the way unsteady perturbations are transferred from the cascade to the far-field.The nature of the waves are first assessed from the aforementioned linearized results,then they are evaluated analytically and finally compared.A good agreement is found despite the strong assumptions of the analytical model.The results show a clear correlation between the cut-on/cut-off conditions and stability.The least stable configuration corresponds to cut-off mode at the inlet and no wave at the outlet.Without outgoing waves from the cascade,the blade is prone to be less stable:the energy from the blades vibration is necessarily dissipated or sent out by the cascade. 展开更多
关键词 subsonic flutter lrans BLISK cut-on/cut-off mode interblade phase angle nodal diameter.
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Mortality pattern according to autopsy findings among traffic accident victims in Yazd, Iran 被引量:2
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作者 Yashar Moharamzad Hamidreza Taghipour +5 位作者 Nader Hodjati Firoozabadi Abolfazl Hodjati Firoozabadi MojtabaHashemzadeh Mehdi Mirjalili and Abed Namavari Mojtaba Hashemzadeh Mehdi Mirjalili Abed Namavari 《Chinese Journal of Traumatology》 CAS 2008年第6期329-334,共6页
Objective: To describe mortality pattern and to determine undiagnosed fatal injuries according to autopsy findings among road traffic accident victims in Yazd, Iran. Methods: In this retrospective study, 251 victims... Objective: To describe mortality pattern and to determine undiagnosed fatal injuries according to autopsy findings among road traffic accident victims in Yazd, Iran. Methods: In this retrospective study, 251 victims of road traffic accidents who were admitted to a tertiary trauma hospital over a two-year period (2006 and 2007) and received medical cares were included. Hospital records were reviewed to gather demographic characteristics, road user type, and medical data. Autopsy records were also reviewed to determine actual causes of death and possible undiagnosed injuries occurred in the initial assessment of the emergency unit or during hospitalization. Results: There were 202 males (80.5%) and 49 females (19.5%). The mean (+SD) age of fatalities was 34.1 (+21.5) years. Pedestrian-vehicle accidents were the most common cause of trauma (100 cases, 39.8%). The most common cause of death was central nervous system injury (146 cases, 58.1%). The other causes were skull base fractures (10%), internalbleeding (8%), lower limb hemorrhage (8%), skull vault fractures (4%), cervical spinal cord injury (3.6%), airway compromise (3.2%), and multifactor cases (5.1%), respectively. Thirty-six fatal injuries in 30 victims (12%) mainly contributed to death according to autopsy, but were not diagnosed in initial assessments. The head (72.2%) and cervical spine (13.8%) regions were the two most common sites for undi- agnosed injuries. Conclusion: Training courses for emergency unit medical staff with regard to interpreting radiological findings of head and neck and high clinical suspicion for cervical spine injuries are essential to improve the quality of early hospital care and reduce the mortality and morbidity of traffic accident patients. 展开更多
关键词 Accidents traffic AUTOPSY Wounds andinjuries lran
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Tectonic Setting of the Barm Firuz Lake,Zagros Mountains,Iran:Inferred from Structural and Karstic Evidence 被引量:1
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作者 Babak Samani 《Journal of Earth Science》 SCIE CAS CSCD 2014年第5期932-938,共7页
This paper presents the role of tectonic and karstic processes in the formation and evolution of Barm Firuz Lake within the Zagros Mountains of Iran. This lake with elevation of 3 340 m from sea level is located in th... This paper presents the role of tectonic and karstic processes in the formation and evolution of Barm Firuz Lake within the Zagros Mountains of Iran. This lake with elevation of 3 340 m from sea level is located in the crest of the Barm Firuz anticline. Results show that the structural evolution of the Barme Firuz Lake is related to both tectonic and karst processes. Therefore the term of tectonokarstic has been used for structural evolution of the lake. Structural studies especially on the stylolite structures revealed the occurrence of an important component of simple shear deformation in the study area. Based on structural and karstic evidence around the lake, the fault-dissolution sink model with oblique pure shear component is suggested as kinematic model of the Barm Firuz Lake evolution in the Zagros Mountains of Iran. 展开更多
关键词 TECTONIC KARST Barm Firuz Lake Zagros Mountains lran.
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Iraq-lran chemical war: calendar, mortality and morbidity
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作者 Seyed Mansour Razavi Mahdiyeh Sadat Razavi +1 位作者 Mohsen Pirhosseinloo Payman Salamati 《Chinese Journal of Traumatology》 CAS CSCD 2014年第3期165-169,共5页
Objectives: To review the calendar, mortality and morbidity of Iraq-Iran chemical war among Iranians based on researchers' reports. Methods: We used national and international databanks such as PubMed, ISI, Scopus... Objectives: To review the calendar, mortality and morbidity of Iraq-Iran chemical war among Iranians based on researchers' reports. Methods: We used national and international databanks such as PubMed, ISI, Scopus, Irandoc and Iranmedex and studied 350 articles related to chemical agents and their effects on different organs. The main criteria for qualification of articles were relevancy orientation and being published in approved medical journals. Results: The Iraqi army invaded to west and southwest Iran using chemical weapons such as nerve agents (NAs) and sulfur mustard (SM). Most victims were civilians including women and children. These attacks had imposed more than 150 types of diseases and complications on Iranians and the frequency of death was 2%-3%. Most reports were about respiratory problems and a few were in the domain of socio-economic damages Conclusion: At present, 25 years after me end of war, the victims are faced with different complications induced by chemical agents and it is estimated that they will be continuously troubled by these problems in future. 展开更多
关键词 Chemical warfare Wounds andinjuries lran lraq
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