This paper summarizes the superiority of lead-rubber beating （LRB） continuous girder bridges. The research method for isolation performance is discussed when pile-soil interaction is considered. By the finite elemen...This paper summarizes the superiority of lead-rubber beating （LRB） continuous girder bridges. The research method for isolation performance is discussed when pile-soil interaction is considered. By the finite element method and self-compiling program, a systematic study of the reliability of LRB continuous girder bridges is given by the use of different indicators, including the riding comfort of the LRB system, the pounding and dynamic stability when the LRB system is subjected to seismic excitations, and the reliability of the LRB system when subjected to other common horizontal loads. The results show that the LRB system has obvious advantages over the traditional continuous girder structure. The LRB isolation effect remains good even when pile-soil interaction is considered; the vertical rigidity of the LRB guarantees desirable riding comfort. The LRB demonstrates good reliability when subjected to the effects of braking, wind loads and temperature. However, it is also pointed out that the pounding of the LRB system subjected to earthquakes must be avoided, and the dynamic stability may be reduced when the LRB system has higher piers and generates a larger displacement in a strong earthquake. Useful advice and guidance are proposed for engineering application.展开更多
Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L., we designed some primers to amplify resis...Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L., we designed some primers to amplify resistant homologous sequences in the near isogenic lines (NILs) of powdery mildew resistance using RT-PCR method. Two expressed cDNA fragments were isolated from wheat genome. One showed 83% homology to the Mlo gene of barley. The other contained two possible open reading frames (ORFs). NBS conservative domains 2, 3 of disease resistance gene and 13 LRR structures similar to rice Pib protein terminal were found respectively in the two ORFs. It indicated that the latter fragment belongs to NBS-LRR-like genes. The obvious difference of RT-PCR products was observed between the before challenged and the challenged for 72 h by Blumeria graminis f. sp. tritici, which, implied that this sequence could be associated with disease resistance of wheat. Using nulli-tetrasomic lines of 'Chinese Spring', the NBS-LRR-like gene had been located on chromosome 1D.展开更多
基金The National Natural Science Foundation of China(No.51008134)
文摘This paper summarizes the superiority of lead-rubber beating （LRB） continuous girder bridges. The research method for isolation performance is discussed when pile-soil interaction is considered. By the finite element method and self-compiling program, a systematic study of the reliability of LRB continuous girder bridges is given by the use of different indicators, including the riding comfort of the LRB system, the pounding and dynamic stability when the LRB system is subjected to seismic excitations, and the reliability of the LRB system when subjected to other common horizontal loads. The results show that the LRB system has obvious advantages over the traditional continuous girder structure. The LRB isolation effect remains good even when pile-soil interaction is considered; the vertical rigidity of the LRB guarantees desirable riding comfort. The LRB demonstrates good reliability when subjected to the effects of braking, wind loads and temperature. However, it is also pointed out that the pounding of the LRB system subjected to earthquakes must be avoided, and the dynamic stability may be reduced when the LRB system has higher piers and generates a larger displacement in a strong earthquake. Useful advice and guidance are proposed for engineering application.
文摘Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L., we designed some primers to amplify resistant homologous sequences in the near isogenic lines (NILs) of powdery mildew resistance using RT-PCR method. Two expressed cDNA fragments were isolated from wheat genome. One showed 83% homology to the Mlo gene of barley. The other contained two possible open reading frames (ORFs). NBS conservative domains 2, 3 of disease resistance gene and 13 LRR structures similar to rice Pib protein terminal were found respectively in the two ORFs. It indicated that the latter fragment belongs to NBS-LRR-like genes. The obvious difference of RT-PCR products was observed between the before challenged and the challenged for 72 h by Blumeria graminis f. sp. tritici, which, implied that this sequence could be associated with disease resistance of wheat. Using nulli-tetrasomic lines of 'Chinese Spring', the NBS-LRR-like gene had been located on chromosome 1D.
