Embryonic stem cells (ES-5) were transfected with PSV 2-neo-EJ-Ras and the cell colonies were chosen in the selection medium containing Geneticin. Southern-blot analysis showed that all the transfected cells possessed...Embryonic stem cells (ES-5) were transfected with PSV 2-neo-EJ-Ras and the cell colonies were chosen in the selection medium containing Geneticin. Southern-blot analysis showed that all the transfected cells possessed the whole EJ-Ras DNA. Northern-blot analysis demonstrated that the selected clones express higher level of Ras mRNA than the control. When the transfected ES-5 cells were injected into the flanks of nude mice, they formed bigger tumors and more stem cell nests were found. The chimeric ability of the transfected ES-5 cells decreased when the cells were injected into the blastocysts which were then transferred back into the uterine of foster mother. We propose that the over-expression of EJ-Ras in ES-5 cells increase the proliferation ability but decreases the chimeric potential of cell.展开更多
AIM: To study the effects of non-cytotoxJc concentrations of docetaxel on some important angiogenic factors of LS174T Cells.METHODS: The non-cytotoxic concentration of docetaxel and the activity of gelatinase were det...AIM: To study the effects of non-cytotoxJc concentrations of docetaxel on some important angiogenic factors of LS174T Cells.METHODS: The non-cytotoxic concentration of docetaxel and the activity of gelatinase were determined with MTT and gelatin zymography respectively, the expression of VEGF (vascular endothelial growth factor), bFGF (basic fibroblast growth factor), MMP (matrix metalloproteinase) 2 and MMP 9 was investigated with RT-PCR and Western blot.RESULTS: The maximum non-cytotoxic concentration of docetaxel on LS174T Cells was 1.0 ng/ml. Compared with the solvent control group, 0.1, 0.5, 1.0 ng/ml of docetaxel could downregulate the expression of VEGF, bFGF, MMP 2and MMP 9 and suppress the activity of gelatinase.CONCLUSION: Our study suggests that the non-cytotoxic concentrations of docetaxel have strong antiangiogenic activity on LS174T Cells, which suggests docetaxel may be a promising antiangiogenic agent.展开更多
文摘Embryonic stem cells (ES-5) were transfected with PSV 2-neo-EJ-Ras and the cell colonies were chosen in the selection medium containing Geneticin. Southern-blot analysis showed that all the transfected cells possessed the whole EJ-Ras DNA. Northern-blot analysis demonstrated that the selected clones express higher level of Ras mRNA than the control. When the transfected ES-5 cells were injected into the flanks of nude mice, they formed bigger tumors and more stem cell nests were found. The chimeric ability of the transfected ES-5 cells decreased when the cells were injected into the blastocysts which were then transferred back into the uterine of foster mother. We propose that the over-expression of EJ-Ras in ES-5 cells increase the proliferation ability but decreases the chimeric potential of cell.
文摘AIM: To study the effects of non-cytotoxJc concentrations of docetaxel on some important angiogenic factors of LS174T Cells.METHODS: The non-cytotoxic concentration of docetaxel and the activity of gelatinase were determined with MTT and gelatin zymography respectively, the expression of VEGF (vascular endothelial growth factor), bFGF (basic fibroblast growth factor), MMP (matrix metalloproteinase) 2 and MMP 9 was investigated with RT-PCR and Western blot.RESULTS: The maximum non-cytotoxic concentration of docetaxel on LS174T Cells was 1.0 ng/ml. Compared with the solvent control group, 0.1, 0.5, 1.0 ng/ml of docetaxel could downregulate the expression of VEGF, bFGF, MMP 2and MMP 9 and suppress the activity of gelatinase.CONCLUSION: Our study suggests that the non-cytotoxic concentrations of docetaxel have strong antiangiogenic activity on LS174T Cells, which suggests docetaxel may be a promising antiangiogenic agent.
