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香蕉类胡萝卜素羟化酶基因MaLUT5克隆与分析 被引量:1
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作者 陈莹 薛丙涛 +6 位作者 李志英 荆永琳 张玄兵 李春燕 颜航 王小冰 徐立 《分子植物育种》 CAS 北大核心 2023年第8期2499-2507,共9页
LUT5 (Lutein deficient 5)负责编码类胡萝卜素羟化酶CYP97A3,参与类胡萝卜素羟基化。为研究LUT5对香蕉果肉类胡萝卜素的调控,本试验以Pilose香蕉叶片为模板,根据NCBI预测的香蕉LUT5序列设计引物进行克隆,并对蛋白质结构进行分析。结果... LUT5 (Lutein deficient 5)负责编码类胡萝卜素羟化酶CYP97A3,参与类胡萝卜素羟基化。为研究LUT5对香蕉果肉类胡萝卜素的调控,本试验以Pilose香蕉叶片为模板,根据NCBI预测的香蕉LUT5序列设计引物进行克隆,并对蛋白质结构进行分析。结果显示,克隆出的MaLUT5 CDS区全序列长为1 971 bp,编码656个氨基酸;MaLUT5蛋白为亲水性不稳定酸性蛋白,主要由α-螺旋和无规则卷曲构成,定位于叶绿体外膜。对Pilose香蕉各个组织进行RT-qPCR检测,结果显示MaLUT5基因具有很强的组织特异性,且在生殖器官的表达水平高于营养器官。此外,对转pBI121-Ma LUT5拟南芥进行GUS组织化学染色分析,结果显示MaLUT5能在拟南芥全株表达。经过完全黑暗处理后,LUT5在AAA基因型中的表达量显著提升,而在BBB基因型的表达量下降。在Pilose变黄的果实中瞬时超表达MaLUT5,MaLUT5表达量和总类胡萝卜素含量显著提高,编码ε-羟化酶CYP97C1的LUT1 (Lutein deficient 1)表达量变化不显著。综上,初步推断LUT5在AAA基因型香蕉果肉类胡萝卜素途径起正调控作用,能促进类胡萝卜素形成。本研究为阐明MaLUT5功能、分子育种培育高类胡萝卜素香蕉提供理论基础。 展开更多
关键词 香蕉 lut5 类胡萝卜素羟化酶 高类胡萝卜素 生物强化
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A new FPGA with 4/5-input LUT and optimized carry chain
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作者 毛志东 陈利光 +1 位作者 王元 来金梅 《Journal of Semiconductors》 EI CAS CSCD 2012年第7期113-120,共8页
A new LUT and carry structure embedded in the configurable logic block of an FPGA is proposed. The LUT is designed to support both 4-input and 5-input structures, which can be configured by users according to their ne... A new LUT and carry structure embedded in the configurable logic block of an FPGA is proposed. The LUT is designed to support both 4-input and 5-input structures, which can be configured by users according to their needs without increasing interconnect resources. We also develop a new carry chain structure with an optimized critical path. Finally a newly designed configurable scan-chain is inserted. The circuit is fabricated in 0.13μm 1P8M 1.2/2.5/3.3 V logic CMOS process. The test results show a correct function of 4/5-input LUT and scan- chain, and a speedup in carry performance of nearly 3 times over current architecture in the same technology at the cost of an increase in total area of about 72.5%. Our results also show that the logic utilization of this work is better than that of a Virtex lI/Virtex 4/Virtex 5/Virtex 6/Virtex 7 FPGA when implemented using only 4-LUT and better than that of a Virtex lI/Virtex 4 FPGA when implemented using only 5-LUT. 展开更多
关键词 FPGA configurable logic block 4/5-input LUT carry chain optimization scan-chain
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