Expression vectors of human granulocyte colony stimulating factor (G-CSG ) and long acting tissue plas-minogen activator (La-tPA) in mammary gland were constructed using promoters of mouse whey acid protein gene (WAP)...Expression vectors of human granulocyte colony stimulating factor (G-CSG ) and long acting tissue plas-minogen activator (La-tPA) in mammary gland were constructed using promoters of mouse whey acid protein gene (WAP) and sheep β-lactoglobulin gene (BLG) with sizes of 2.6 and 5 kb respectively. Two kinds of transgenic mice of G-CSF and La-tPA were produced with microinjection. The expression of G-CSF and La-tPA was achieved in mammary glands of transgenic mice, respectively. In order to establish dual transgenic mice of La-tPA /G-CSF, transgenic mice carrying G-CSF and La-tPA gene characterized with specific expression in mammary gland were mated. La-tPA/G-CSF dual transgenic mice were screened out from the hybrid offspring by Once-PCR. The co-expression of La-tPA and G-CSF in mammary gland of the dual transgenic mice was confirmed by the milk assayed and Northern blot analysis. Some parameters about the dual transgenic mice indicated that there were fewer litters than that of normal mice. The ratio of dual transgenes was 46.1 % in Fl generation, and offspring s sex ratio was normal. Hence a dual transgenic mouse model was established for the study of co-expression foreign proteins in mammary gland.展开更多
A distributed local adaptive transmit power assignment (LA-TPA) strategy was proposed to construct a topology with better performance according to the environment and application scenario and prolong the network lifet...A distributed local adaptive transmit power assignment (LA-TPA) strategy was proposed to construct a topology with better performance according to the environment and application scenario and prolong the network lifetime.It takes the path loss exponent and the energy control coefficient into consideration with the aim to accentuate the minimum covering district of each node more accurately and precisely according to various network application scenarios.Besides,a self-healing scheme that enhances the robustness of the network was provided.It makes the topology tolerate more dead nodes than existing algorithms.Simulation was done under OMNeT++ platform and the results show that the LA-TPA strategy is more effective in constructing a well-performance network topology based on various application scenarios and can prolong the network lifetime significantly.展开更多
基金Project supported by the National High Technology Developmental Program "863".
文摘Expression vectors of human granulocyte colony stimulating factor (G-CSG ) and long acting tissue plas-minogen activator (La-tPA) in mammary gland were constructed using promoters of mouse whey acid protein gene (WAP) and sheep β-lactoglobulin gene (BLG) with sizes of 2.6 and 5 kb respectively. Two kinds of transgenic mice of G-CSF and La-tPA were produced with microinjection. The expression of G-CSF and La-tPA was achieved in mammary glands of transgenic mice, respectively. In order to establish dual transgenic mice of La-tPA /G-CSF, transgenic mice carrying G-CSF and La-tPA gene characterized with specific expression in mammary gland were mated. La-tPA/G-CSF dual transgenic mice were screened out from the hybrid offspring by Once-PCR. The co-expression of La-tPA and G-CSF in mammary gland of the dual transgenic mice was confirmed by the milk assayed and Northern blot analysis. Some parameters about the dual transgenic mice indicated that there were fewer litters than that of normal mice. The ratio of dual transgenes was 46.1 % in Fl generation, and offspring s sex ratio was normal. Hence a dual transgenic mouse model was established for the study of co-expression foreign proteins in mammary gland.
基金Projects(61101104,61100213) supported by the National Natural Science Foundation of ChinaProject(NY211050) supported by Fund of Nanjing University of Posts and Telecommunications,China
文摘A distributed local adaptive transmit power assignment (LA-TPA) strategy was proposed to construct a topology with better performance according to the environment and application scenario and prolong the network lifetime.It takes the path loss exponent and the energy control coefficient into consideration with the aim to accentuate the minimum covering district of each node more accurately and precisely according to various network application scenarios.Besides,a self-healing scheme that enhances the robustness of the network was provided.It makes the topology tolerate more dead nodes than existing algorithms.Simulation was done under OMNeT++ platform and the results show that the LA-TPA strategy is more effective in constructing a well-performance network topology based on various application scenarios and can prolong the network lifetime significantly.
