The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 d...The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 dimethylthiazol 2 yl) 2,5 diphenytetrazolium bromide) assay, 3 H TdR incorporation, flow cytometry, fluorescence polarization assay, and soft agar culture. The results indicate that when the concentrations of LaCit are 0.05, 0.1, 0.5 and 1 mmol·L -1 , it inhibits PG cells proliferation. When the concentrations are 0.5 and 1 mmol·L -1 LaCit inhibits PG cells 3 H TdR incorporation, and decreases the proportion of PG cells in S phase while increases the proportion of PG cells in G 1 phase and decreases the membrane fluidity of PG cells. But LaCit has no effect on BGC 823 cells except for cell membrane fluidity and no effect on 2BS cells.展开更多
文摘The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 dimethylthiazol 2 yl) 2,5 diphenytetrazolium bromide) assay, 3 H TdR incorporation, flow cytometry, fluorescence polarization assay, and soft agar culture. The results indicate that when the concentrations of LaCit are 0.05, 0.1, 0.5 and 1 mmol·L -1 , it inhibits PG cells proliferation. When the concentrations are 0.5 and 1 mmol·L -1 LaCit inhibits PG cells 3 H TdR incorporation, and decreases the proportion of PG cells in S phase while increases the proportion of PG cells in G 1 phase and decreases the membrane fluidity of PG cells. But LaCit has no effect on BGC 823 cells except for cell membrane fluidity and no effect on 2BS cells.
