Labisia pumila(Kacip fatimah)is a popular medicinal plant in Malaysia.The constituents of this plant have been reported to possess anticancer,antioxidant and anti-inflammatory properties.The growth and production of L...Labisia pumila(Kacip fatimah)is a popular medicinal plant in Malaysia.The constituents of this plant have been reported to possess anticancer,antioxidant and anti-inflammatory properties.The growth and production of L.pumila is greatly influenced by the plant water status.Current techniques to measure plant water status are generally based on the plant soil moisture,which apparently did not indicate the real water content inside the plant.There are other techniques to measure directly on the plant such as based on leaf water potential(LWP)and relative water content(RWC).However,these techniques are destructive and time consuming.In this study,four levels of evapotranspiration replacement(ER)treatment which were 100%ER,75%ER,50%ER and 25%ER was applied to 30 polybags of L.pumila plants.The plant water status was measured using an impedance spectroscopy technique.A pair of electrocardiogram(ECG)electrode connected to an impedance analyzer board was used to measure the impedance value of the leaf samples noninvasively.Plant water status parameters such as LWP,RWC,volumetric moisture content(VMC),and leaf thickness were measured using standard methods.The results show that after 20 weeks of treatment,25%ER had the highest impedance value ranged from 0.10 MX to 0.15 MX at the frequency of 70–100 kHz.The resistance of 100%ER at 20 weeks of treatment increased from 0.70 kX to 1.23 kX as the reactance decreased from 0.51 kX to 0.28 kX.Comparatively,the resistance of 25%ER increased from 1 kX to 1.10 kX as the reactance decreased from 0.88 kX to 0.83 kX.The polynomial regression of impedance measurements with plant water status parameters(VMC,leaf thickness,LWP and RWC)shows that LWP and RWC had the highest R2(0.78,0.73).The results show that impedance measurement technique is auspicious to evaluate plant water status.展开更多
Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medici...Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.展开更多
基金The authors gratefully acknowledge the financial support from the Ministry of Education Malaysia and Universiti Putra Malaysia with the UPM Research Grant Scheme(Project Code:GP-IPS/2013/9399814)The authors would also like to acknowledge Mrs.Nadirah Roslan who was involved in data collection process.
文摘Labisia pumila(Kacip fatimah)is a popular medicinal plant in Malaysia.The constituents of this plant have been reported to possess anticancer,antioxidant and anti-inflammatory properties.The growth and production of L.pumila is greatly influenced by the plant water status.Current techniques to measure plant water status are generally based on the plant soil moisture,which apparently did not indicate the real water content inside the plant.There are other techniques to measure directly on the plant such as based on leaf water potential(LWP)and relative water content(RWC).However,these techniques are destructive and time consuming.In this study,four levels of evapotranspiration replacement(ER)treatment which were 100%ER,75%ER,50%ER and 25%ER was applied to 30 polybags of L.pumila plants.The plant water status was measured using an impedance spectroscopy technique.A pair of electrocardiogram(ECG)electrode connected to an impedance analyzer board was used to measure the impedance value of the leaf samples noninvasively.Plant water status parameters such as LWP,RWC,volumetric moisture content(VMC),and leaf thickness were measured using standard methods.The results show that after 20 weeks of treatment,25%ER had the highest impedance value ranged from 0.10 MX to 0.15 MX at the frequency of 70–100 kHz.The resistance of 100%ER at 20 weeks of treatment increased from 0.70 kX to 1.23 kX as the reactance decreased from 0.51 kX to 0.28 kX.Comparatively,the resistance of 25%ER increased from 1 kX to 1.10 kX as the reactance decreased from 0.88 kX to 0.83 kX.The polynomial regression of impedance measurements with plant water status parameters(VMC,leaf thickness,LWP and RWC)shows that LWP and RWC had the highest R2(0.78,0.73).The results show that impedance measurement technique is auspicious to evaluate plant water status.
文摘Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.
