Mucosal healing effect of mesalazine granules in naproxen-induced small bowel enteropathy

AIM:To investigate the effect of mesalazine granules on small intestinal injury induced by naproxen using capsule endoscopy (CE).METHODS:This was a single center,non-randomized,open-label,uncontrolled pilot study,using the PillCam SB CE system with RAPID 5 software.The Lewis Index Score (LIS) for small bowel injury was investigated to evaluate the severity of mucosal injury.Arthropathy patients with at least one month history of daily naproxen use of 1000 mg and proton pump inhibitor co-therapy were screened.Patients with a minimum LIS of 135 were eligible to enter the 4-wk treatment phase of the study.During this treatment period,3 × 1000 mg/d mesalazine granules were added to ongoing therapies of 1000 mg/d naproxen and 20 mg/d omeprazole.At the end of the 4-wk combined treatment period,a second small bowel CE was performed to re-evaluate the enteropathy according to the LIS results.The primary objective of this study was to assess the mucosal changes after 4 wk of mesalazine treatment.RESULTS:A total of 18 patients (16 females),ranging in age from 46 to 78 years (mean age 60.3 years) were screened,all had been taking 1000 mg/d naproxen for at least one month.Eight patients were excluded from the mesalazine therapeutic phase of the study for the following reasons:the screening CE showed normal small bowel mucosa or only insignificant damages (LIS < 135) in five patients,the screening esophagogastroduodenoscopy revealed gastric ulcer in one patient,capsule technical failure and incomplete CE due to poor small bowel cleanliness in two patients.Ten patients (9 female,mean age 56.2 years) whose initial LIS reached mild and moderate-to-severe enteropathy grades (between 135 and 790 and ≥ 790) entered the 4-wk therapeutic phase and a repeat CE was performed.When comparing the change in LIS from baseline to end of treatment in all patients,a marked decrease was seen (mean LIS:1236.4 ± 821.9 vs 925.2 ± 543.4,P=0.271).Moreover,a significant difference between pre-and post-treatment mean total LIS was detected in 7 patients who had moderate-tosevere enteropathy gradings at the inclusion CE (mean LIS:1615 ± 672vs 1064 ± 424,P=0.033).CONCLUSION:According to the small bowel CE evaluation mesalazine granules significantly attenuated mucosal injuries in patients with moderate-to-severe enteropathies induced by naproxen.

Effect of Simiao Liangxue Granule on Acute Gouty Arthritis in Rats

[Objectives]To study the anti-inflammatory effect of Simiao Liangxue granule on the rats with acute gouty arthritis(AGA).[Methods]A total of 60 male SD rats were randomly divided into 6 groups with 10 rats in each group.The rats in the colchicine group were intragastrically administered with 0.65 mg/kg colchicine,and the rats in low,medium and high dose groups of Simiao Liangxue granule were intragastrically administered with Simiao Liangxue granule with mass concentration of 0.4,0.8 and 1.6 g/kg,respectively.The rats in the normal control group and the model group were given the same amount of normal saline for 7 d,and the acute gouty arthritis model was prepared 1 h after administration on the 5th d.At the end of the experiment,the degree of joint swelling and joint inflammation index,the content of interleukin-1β,tumor necrosis factor-a and cyclooxygenase-2 in articular cartilage,and the content of interleukin-8,NO and UA in serum of rats were measured,and the histomorphological observation was performed.[Results]Compared with the normal control group,the joints of rats were swollen to different degrees,the inflammation score was significantly increased,and the content of IL-1β,TNF-αand COX-2 in the soft tissue of the joints was significantly increased in each group(P<0.05);compared with the model group,the joint swelling degree and inflammation score of rats in the colchicine group and Simiao Liangxue granule group were significantly decreased,the content of IL-1β,TNF-αand COX-2 in the joint soft tissue was also significantly decreased(P<0.05),and the indexes of each dose group of Simiao Liangxue granule were similar to those of the colchicine group;compared with the normal control group,the content of IL-8,NO and UA in the joint soft tissue of rats was significantly increased in each group(P<0.05);compared with the model group,the content of all indexes in joint soft tissue of rats in normal control group,colchicine group and Simiao Liangxue granule group were significantly decreased(P<0.05);the content of various indexes in the joint soft tissue of rats in the low and medium dose groups of Simiao Liangxue granule was higher than that in the colchicine group,and decreased significantly with the increase of the dose of Simiao Liangxue granule(P<0.05);the inflammatory index decreased significantly with the increase of the dose of Simiao Liangxue granule(P<0.05);the inflammation and degeneration in each dose group of Simiao Liangxue granule were milder than those in the model group,and the symptoms were relieved to some extent with the increase of dose(P<0.05).[Conclusions]Simiao Liangxue granule has the effect of anti-inflammation and detumescence,and its mechanism may be related to the content of factors that reduce inflammation including IL-1β,TNF-α,C0X-2,IL-8,NO and UA.

Experimental Study on Pharmacodynamic of Qingyan Granules

Objective: To investigate the pharmacodynamics of Qingyan granules. Methods: To investigate the efficancy of qingyan granules, the animal experience of maximum tolerated dose of mice was made. And the auricular swelling model of mice, the foot swelling model of rat, the phenol red secretion method, and the hot-plate and glacial acetic acid stimulation writhing method was established and used to test the anti-inflammatory effect, the expectorant effect, and the analgesia effect. Results: Pharmacodynamics experiment proved that the maximum dosage for mice was 42g/kg, the equivalent of 56 times for human. Qingyan granules has obvious inhibitory effect on mouse auricle swelling caused by xylene, and has obvious inhibitory effect on carrageenan-induced rat paw edem. Also, the mice pain caused by thermal and chemical stimulation has obviously inhibited by Qingyan granules. Qingyan granules can prolong the pain reaction time on mice caused by acetic acid. Conclusions: Qingyan granules have evident effects of anti-inflammatory effect, analgesic effect and expectorant effect.

补血生乳颗粒联合低频脉冲电刺激对产后缺乳患者疗效的临床研究

目的探究补血生乳颗粒联合低频脉冲电刺激对产后缺乳患者的临床效果。方法选取2022年1月至2023年9月赣州市妇幼保健院收治的120例产后缺乳患者作为研究对象,根据随机数字表法分为试验组1、试验组2、试验组3,每组各40例。所有患者均接受常规治疗,在此基础上,试验组1患者行低频脉冲电刺激治疗,试验组2患者行补血生乳颗粒治疗,试验组3患者行补血生乳颗粒联合低频脉冲电刺激治疗。比较三组患者的乳房充盈程度、泌乳量、红细胞计数与血红蛋白水平以及治疗效果。结果治疗后2、4周,试验组3的乳房充盈程度评分低于试验组1与试验组2,泌乳量高于试验组1与试验组2,差异有统计学意义(P<0.05)。治疗后2、4周,试验组3的红细胞计数与血红蛋白水平均高于试验组1与试验组2,差异有统计学意义(P<0.05)。三组患者的总有效率比较,差异有统计学意义(P<0.05);试验组3的治疗总有效率高于试验组1,差异有统计学意义(P<0.017)。结论将补血生乳颗粒与低频脉冲电刺激联合应用于产后缺乳患者的治疗中,可有效改善乳汁分泌情况,并能够对患者起到补血益气的作用,有利于促进患者生化指标改善,值得临床推广。

Anti-inflammatory,anti-tussive effects and toxicity evaluation of Qingfei Dayuan granules(清肺达原颗粒)

OBJECTIVE:To study the anti-inflammatory and anti-tussive effects of Qingfei Dayuan granules(清肺达原颗粒,QFDY),and to evaluate the acute and sub-chronic toxicity of QFDY.METHODS:Anti-inflammatory effects were evaluated by murine model of xylene induced ear edema in mice.Ear swelling degree was calculated and tumor necrosis factor-α,interleukin-1βand interleukin-6 were determined.Anti-tussive evaluations were carried out in the mouse cough model induced by ammonia liquor.Latent period cough and number of cough within 3 min were counted.In acute toxicity study,the rats were randomly divided into test group and solvent control group.Body weighs,food intakes and general clinical signs were monitored.In the sub-chronic toxicity study,QFDY was administered to rats at 0,4,8 and 16 g/kg per day for 28 and 30 d of post treatment was conducted.Mortalities,clinical signs,body weight changes,food intakes,ophthalmological examinations,hematological parameters,biochemical indicators,electrolyte indicators,urinalyses and histopathological examinations were monitored.RESULTS:QFDY significantly inhibited the development of ear edema in anti-inflammatory assay and decreased cough frequency caused by ammonia liquor.The results presented a dose-effect relationship.In acute toxicity study,no abnormality exhibited at dose of 24.0 g/kg per day during the 14-d observation period.In the sub-chronic toxicity study,higher reticulocyte count,lymphocyte and lower Cl-,blood urea nitrogen were analyzed compared with the solvent control group.But the differences were considered to be incidental and not clinically toxic.Obvious dose-effect relationship of urine color was observed,and the three test groups at the end of the experiments resulted in significant increase in urobilinogen,bilirubin,ketone body and urine leukocyte.However,all the positive indicators returned to normal in the recovery period.Therefore,no toxicological changes were found during the study period.CONCLUSION:QFDY showed significant anti-inflammatory and anti-tussive effects in mice.The lethal dose(LD50)of per oral QFDY in rats was estimated to be more than 24.0 g/kg per day and the no observed adverse effect level was over 16 g/kg per day,which suggested that QFDY is relatively safe for oral medication at the present dose on rats.Our experimental results provide a reference for the further development and research of QFDY.

调肝清心冲剂治疗室性早搏分子机制研究

目的:探究调肝清心冲剂治疗室性早搏的分子机制。方法:将40只SD大鼠按随机数字表法分为假手术组、模型组、稳心颗粒组、调肝清心冲剂治疗组四组,每组10只大鼠(雌雄各5只)。假手术组采用0.9%NaCl溶液等剂量灌胃;模型组采用0.9%NaCl溶液等剂量灌胃;稳心颗粒组采用1 ml/100 g(相当于4.28 g生药/kg体重)的剂量每天1次灌胃,在手术前7天开始;调肝清心冲剂治疗组,1 ml/100g(相当于4.28 g生药/kg体重)的剂量每天1次灌胃,在手术前7天开始。在灌注后30 min内通过心电图监测记录各组室性早搏出现的时间和持续时间、室性早搏(VP)和室性心动过速(VT)的发生频率,并采用Mest法评估心律失常评分,心电图检测完毕后立即采用速率法测定血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)水平;Western blot法测定大鼠左室心肌组织细胞间隙连接蛋白43(Cx43)的表达。结果:与假手术组、模型组、稳心颗粒组比较,调肝清心冲剂治疗组的室性早搏出现时间增加和持续时间明显降低,差异有统计学意义(P<0.05)。调肝清心冲剂治疗组的室性早搏和室性心动过速的发生率均低于假手术组、模型组、稳心颗粒组及调肝清心冲剂治疗组,差异有统计学意义(P<0.05)。与假手术组、模型组、稳心颗粒组比较,调肝清心冲剂治疗组的Mest评分明显降低,差异有统计学意义(P<0.05)。与假手术组、模型组、稳心颗粒组比较,调肝清心冲剂治疗组的血清LDH、CK水平明显降低,差异有统计学意义(P<0.05)。与假手术组、模型组、稳心颗粒组比较,调肝清心冲剂治疗组的Cx43含量明显增高,差异有统计学意义(P<0.05)。结论:调肝清心冲剂能够降低心肌缺血模型大鼠心律失常的发生率,其机制可能与降低LDH、CK水平,增加Cx43含量有关。

VSD联合rb-bFGF在慢性难愈合创面中的应用效果分析

目的探讨负压封闭引流(VSD)联合重组牛碱性成纤维细胞生长因子(rb-bFGF)在慢性难愈合创面中的应用效果。方法选取2020年2月至2022年2月许昌中医院收治的100例慢性难愈合创面患者作为研究对象,按照不同治疗方法将其分为联合组(50例)和VSD组(50例),联合组患者局部创面采用VSD联合rb-bFGF治疗,VSD组患者局部创面单纯采用VSD治疗,对比观察两组患者创面肉芽组织成熟度、氧分压、血清乳酸脱氢酶(LDH)与琥珀酸脱氢酶(SDH)水平及VSD敷料更换次数。结果治疗2周后,联合组患者肉芽组织成熟度为Ⅰ度3例、Ⅱ度11例、Ⅲ度15例、Ⅳ度21例,明显优于VSD组患者的肉芽组织成熟度为Ⅰ度17例、Ⅱ度8例、Ⅲ度16例、Ⅳ度9例(Z=-3.270,P<0.001);治疗2周后,联合组患者创面氧分压及血清SDH水平均明显高于VSD组(t=17.682、2.653,P<0.001、P=0.009),而LDH水平明显低于VSD组(t=10.038,P<0.001);联合组患者VSD敷料更换次数为(3.62±0.53)次,明显少于VSD组患者的VSD敷料更换次数(5.13±0.61)次(t=13.210,P<0.001)。结论与单纯应用VSD治疗相比,VSD联合rb-bFGF治疗更有利于提高慢性难愈合创面肉芽组织成熟度及SDH活性,降低LDH活性,改善创面局部微环境,缩短治疗时间。

泌乳宝冲剂催乳作用的实验研究

目的研究泌乳宝冲剂的催乳作用。 [方法 ]通过对母鼠每天腹腔注射NaF 15× 10 - 6 注射体积 10mL/kg ,连续 7d观察小鼠动物缺乳模型及受试药物和正常产褥期乳汁分泌量的影响 ,用放免方法检测血清泌乳素水平并观察组织学改变。 [结果 ]泌乳宝冲剂 6× 10 - 3、12× 10 - 3灌胃 (ig)给药对NaF致小鼠缺乳模型均有显著增加乳量的作用 (P <0 .0 1) ,可明显增加缺乳动物血清泌乳素的含量 (P <0 .0 5 ) ,使乳腺组织呈高度泌乳状态。对正常小鼠母鼠乳汁量及仔鼠体重也有明显增加作用。 [结论 ]泌乳宝冲剂对缺乳状态下的小鼠乳汁分泌有明显作用。

乳泉冲剂对实验性泌乳不足大鼠泌乳量的影响

目的 评价乳泉冲剂对实验性泌乳不足大鼠泌乳量的影响。方法 取Wistar大鼠 ,随机分为 6组 ,选用己烯雌酚建立实验性泌乳不足模型。各组母鼠自产后当天起 ,每日给药一次 ,连续 10d ,记录母鼠的泌乳量 ,用放射免疫法测定母鼠血清催乳素 (PRL)水平 ,并取乳腺活检。结果 乳泉冲剂可使母鼠泌乳量明显增多 ,仔鼠体重增长加速 ,且母鼠的血清催乳素分泌水平有所提高。

通腑颗粒对犬缺血/再灌注损伤小肠黏膜血液灌流和通透性的影响

目的:观察通腑颗粒对肠缺血/再灌注(I/R)损伤小肠黏膜血液灌流及通透性的影响。方法:采用夹闭肠系膜上动脉1 h后恢复血液灌流造成肠I/R损伤犬动物模型。随机将动物分成I/R模型组和I/R治疗组,每组8只。缺血即刻经肠内营养通道分别滴注生理盐水或中药通腑颗粒溶液(0.3 g/kg)。于缺血前及缺血后1、6、8、12和24 h检测血浆二胺氧化酶(DAO)活性、D-乳酸含量、肠黏膜二氧化碳分压(P iCO2);于缺血后24 h活杀动物,取空肠组织,观察病理形态学改变。结果:两组缺血后P iCO2、血浆DAO和D-乳酸均较缺血前显著升高,但随缺血时间延长,各指标有所下降。I/R治疗组各指标均明显低于I/R模型组,且于缺血后24 h回落至缺血前水平。光镜下I/R模型组缺血早期小肠黏膜下血管明显增多,黏膜血管扩张、充血,并可见大量炎性细胞浸润;黏膜上皮细胞坏死、脱落,肠壁变薄。I/R治疗组肠组织病理损害较I/R模型组减轻。结论:通腑颗粒能显著改善肠组织血液灌流及功能指标,减轻肠黏膜病理损害,对I/R损伤小肠有显著的保护作用。

消炎泌乳冲剂对隐性乳房炎奶牛血清中IL-8、TNF-α的影响

选择无乳房炎临床症状且乳汁检测体细胞数大于50万/mL,CAM检测阳性的奶牛60头,依据年龄、胎次相同,泌乳日相似(产犊日期在5d以内),产奶量和泌乳期相近的原则进行配对分组。治疗组用消炎泌乳冲剂300-400g头/d,对照组不用药。结果表明:消炎泌乳冲剂可以使乳房炎奶牛血清中的IL-8、TNF-α的含量显著降低,用药后14d,IL-8的含量与试验前和对照组比,分别降低0.194ng/L和0.198ng/L,差异显著(P<0.05),用药21d分别降低0.263ng/L和0.253ng/L,用药30d分别降低0.268ng/L和0.239ng/L,差异极显著(P<0.01);TNF-α含量在用药14d、21d和30d与试验前相比,分别降低0.33ng/L、0.459ng/L和0.646ng/L,差异极显著(P<0.01),和对照组比分别降低0.372ng/L、0.504ng/L和0.678ng/L,差异极显著(P<0.01)。乳汁中的体细胞数在用药后14d极显著下降(P<0.01),21d时下降到50万/mL以下,到120d时一直维持在正常水平内。治疗组奶牛在试验期间每头牛的日产奶量比对照组提高10.98%(P<0.05),停药后3个月对照组日产奶量比治疗组降低了39.97%(P<0.01)。受试奶牛乳脂率和乳蛋白没有显著性变化。

五种医院制剂菌检的抑菌作用消除方法学考察

目的:建立5种医院制剂微生物限度检查和无菌检查抑菌性消除的方法学研究。方法:以大肠埃希杆菌、金黄色葡萄球菌和白念珠菌等菌株为试验菌株,对感冒安颗粒、愈肠颗粒、护肤粉刺霜、碘氯己定溶液和复方乳酸依沙吖啶散5种医院制剂,进行样品抑菌性消除方法研究,以菌落计数回收率>70%的样品处理方法为微生物限度检查抑菌性消除方法;试验菌落生长状况良好的样品处理方法为无菌检查的抑菌性消除方法。结果:感冒安颗粒、愈肠颗粒和护肤粉刺霜分别采用常规法和供试品稀释法,大肠埃希杆菌、金黄色葡萄球菌和白念珠菌的菌落回收率>70%;碘氯己定溶液联用中和法和薄膜过滤法,复方乳酸依沙吖啶散联用离心沉淀法和薄膜过滤法,消除它们对所研究的3种试验菌株的抑菌性。结论:5种制剂所抑制的菌株不同,抑制作用的强度也不同,需采用不同的方法进行抑菌性消除。

乳宁颗粒联合按摩疏通法治疗肝郁气滞型急性哺乳期乳腺炎临床研究

目的观察乳宁颗粒联合按摩疏通法治疗肝郁气滞型急性哺乳期乳腺炎临床疗效以及血清白细胞计数(WBC)、降钙素原(PCT)、C-反应蛋白(CRP)、雌二醇(E_(2))、孕酮(P)、催乳素(PRL)指标的变化情况,探讨其治疗急性哺乳期乳腺炎的机制。方法采用随机对照方法,对门诊就诊的180例肝郁气滞型急性哺乳期乳腺炎患者分为两组,各90例。治疗组在对照组的基础上加用2周乳宁颗粒冲剂口服,观察两组治疗前后的临床治疗效果、治愈时间、转归情况及复发性的相关指标和各项实验室指标。结果两组比较治疗组治疗周期短、使用抗生素率低、复发率低、发生脓肿率低、被动停乳率低:治疗组临床总有效率94.44%(85/90),明显高于对照组80.00%(72/90)的有效率(P<0.01);治疗组血清WBC、PCT、CRP恢复正常时间明显短于对照组;治疗组血清E、P、PRL水平比对照组明显提高,差异均有统计学意义(P<0.01)。结论乳宁颗粒联合按摩疏通法治疗肝郁气滞型急性哺乳期乳腺炎比单纯按摩法有较好的疗效,改善临床症状缩短治疗时间,缓解患者抑郁情绪,有效降低患者血清WBC相关炎性指标,提高有效的激素水平,有利于早期治愈,减少复发率和停乳率。

正心冲剂的心血管作用和耐缺氧作用研究

用正心冲剂1.5mg/ml、4.5mg/ml、9mg/ml 灌流离体豚鼠心脏,能明显增强心肌收缩幅度,增加冠脉流量,心率改变不明显。减少缺氧再给氧心脏 LDH 释放。同时正心冲剂80mg/10g 和160mg/10g 可明显延长小鼠常压下耐缺氧时间。提示正心冲剂对心肌保护作用的机制可能与改善心肌功能,减轻缺氧时心肌损伤有关。

微波辐射下活性炭固载对甲苯磺酸催化合成乳酸正丁酯

利用颗粒状活性炭固栽对甲苯磺酸作催化剂,在微波辐射下,快速合成乳酸正丁酯。实验结果显示。当微波 功率为300w,催化剂用量为0.8 g,醇酸物质的量的经为3:1,反应时间仅为30min时,酯化率可达97.0％以上。另 外,通过实验发现,微波辐射下,反应速率明显高于常规加热方式。

益宫颗粒联合缩宫素治疗剖宫产术后子宫复旧不全临床效果及对泌乳的影响

目的探讨益宫颗粒联合缩宫素在剖宫产术后子宫复旧不全治疗中的应用效果。方法将2016年10月—2018年12月收治的118例剖宫产术后子宫复旧不全患者以随机数字表法分为对照组59例、观察组59例,对照组给予宫缩素治疗,观察组在对照组基础上加用益宫颗粒。观察两组治疗效果、症状改善情况、治疗7 d后泌乳情况及不良反应发生率。结果观察组治疗总有效率为94.92%(56/59),与对照组81.36%(48/59)对比,明显较高(P<0.05);观察组治疗后恶露停止时间、腰腹痛消失时间与对照组对比,明显较短,平均恶露量、子宫三径和与对照组对比,明显较小(P<0.05);治疗7 d后,观察组治疗泌乳充足率为91.53%(54/59),与对照组76.27%(45/59)对比,明显较高(P<0.05);用药后观察组不良反应发生率为11.86%(7/59),与对照组8.47%(5/59)对比,无明显差异(P>0.05)。结论益宫颗粒联合缩宫素治疗剖宫产术后子宫复旧不全,疗效确切,可显著促进患者症状恢复,改善患者泌乳情况,且安全性高,值得推广。

散结颗粒剂湿敷治疗儿童胞生痰核

目的探讨散结颗粒剂湿敷对儿童胞生痰核的疗效。方法将100例年龄4~10岁,发病5 d以上儿童胞生痰核患者随机分为2组,对照组采用乳酸依沙吖啶溶液湿敷,治疗组采用散结颗粒剂湿敷。2组都配合妥布霉素滴眼液点眼。治疗1个疗程后,比较2组疗效。结果治疗组治愈16例,好转30例,无效4例,总有效率92%;对照组治愈8例,好转26例,无效16例,总有效率68%,治疗组明显优于对照组(P<0.01)。结论散结颗粒剂湿敷可有效儿童胞生痰核临床症状。

维生素D滴剂预防小儿活动性佝偻病的价值分析

目的探讨小儿活动性佝偻病应用维生素D滴剂预防的价值。方法选择我院2016年1月～2017年10月收治的300例活动性佝偻病预防性用药小儿进行研究,按照随机数字表法分为观察组(150例)与对照组(150例)。对照组给予碳酸钙颗粒预防活动性佝偻病,持续用药半年;观察组给予碳酸钙颗粒+维生素D滴剂预防活动性佝偻病,持续用药半年。比较两组小儿用药前后的25-羟维生素D3[25-(OH)D3]、血钙、血磷、脱氧吡啶诺啉交联(DPD)、骨碱性磷酸酶(BALP)]血清生化指标水平,记录两组的不良反应发生率以及随访1年时的25-(OH)D3、活动性佝偻病发生率、骨密度、BALP。结果两组用药前的25-(OH)D3、血钙、血磷、DPD、BALP水平比较,差异无统计学意义(P>0.05)。观察组用药后的25-(OH)D3、血钙、血磷、DPD、BALP水平均高于对照组,差异有统计学意义(P<0.05)。两组的不良反应发生率比较,差异无统计学意义(P>0.05)。观察组随访1年时的25-(OH)D3、骨密度、BALP水平均高于对照组,而活动性佝偻病发生率低于对照组,差异有统计学意义(P<0.05)。结论维生素D滴剂预防小儿活动性佝偻病有着较好的价值,同时还可改善小儿血清生化指标、骨密度等,有较高的安全性,值得应用。

催乳方中药饮片与配方颗粒质量鉴定及治疗产后缺乳症疗效比较

目的:对催乳方中药饮片与配方颗粒进行质量鉴定,并对其在产后缺乳症临床上的疗效进行分析比较。方法:选取催乳方中药饮片和配方颗粒各3个批次进行质量鉴定。选取产后缺乳症患者40例,随机分为配方颗粒组和中药饮片组,各20例,分别给与催乳方配方颗粒溶液和中药饮片水煎液治疗,观察治疗效果。结果:质量鉴定结果显示,与中药饮片水煎液相比,配方颗粒溶液的气味较轻颜色深。中药饮片水煎液中,人参皂苷Rg1、毛蕊异黄酮葡萄糖苷和阿魏酸的含量显著高于配方颗粒溶液(P<0.05);而人参皂苷Re的含量明显低于配方颗粒溶液(P<0.05)。中药饮片水煎液各种成分的相对标准偏差(RSD)均显著高于配方颗粒。临床疗效结果显示,催乳方颗粒剂和中药饮片均能显著增加乳汁分泌量(P<0.05),且两者之间疗效无显著性差异(P>0.05);配方颗粒组和中药饮片组的总有效率分别为90.0%和85.0%,疗效显著,但两者之间无差异(P>0.05)。结论:催乳方配方颗粒溶液质量分析结果较好,对产后缺乳症具有良好的效果,与中药饮片水煎液疗效基本相同。

Postpartum meloxicam administration alters plasma haptoglobin, polyunsaturated fatty acid, and oxylipid concentrations in postpartum ewes

Background: Postpartum inflammation is a natural and necessary response;however, a dysfunctional inflammatory response can be detrimental to animal productivity. The objective of this study was to determine the effects of a non-steroidal anti-inflammatory drug(meloxicam) on ewe postpartum inflammatory response, ewe plasma polyunsaturated fatty acid and oxylipid concentrations, and lamb growth.Results: After lambing, 36 Hampshire and Hampshire × Suffolk ewes were sequentially assigned within type of birth to control(n = 17) or meloxicam orally administered on d 1 and 4 of lactation(MEL;90 mg, n = 19). Milk and blood samples were collected on d 1(prior to treatment) and d 4. Milk glucose-6-phosphate was not affected by MEL. Plasma haptoglobin(Hp) concentrations were less for MEL ewes;control ewes with greater d 1 Hp concentrations had elevated Hp on d 4, but this was not the case for MEL-treated ewes. Treatment with MEL increased plasma arachidonic acid concentration by more than 4-fold in ewes rearing singles but decreased concentrations of 9,10-dihydroxyoctadecenoic acid, prostaglandin F2α, 8-iso-prostaglandin E2, and 8,9-dihydroxyeicosatetraenoic acid. Nine oxylipids in plasma had interactions of treatment with d 1 Hp concentration,all of which revealed positive associations between d 1 Hp and d 4 oxylipid concentrations for CON, but neutral or negative relationships for MEL. MEL decreased 13-hydroxyoctadecadienoic acid:13-oxooctadecadienoic acid ratio and tended to increase 9-hydroxyoctadecadienoic acid:9-oxooctadecadienoic acid ratio(both dependent on d 1 values), indicating progressive metabolism of linoleic acid-derived oxylipids occurred by enzymatic oxidation after MEL treatment. Meloxicam reduced oxylipids generated across oxygenation pathways, potentially due to an improved redox state.Conclusions: Postpartum MEL treatment of ewes decreased plasma concentrations of Hp and several oxylipids,with the greatest impact in ewes with biomarkers reflecting a greater inflammatory state before treatment. Antiinflammatory strategies may help resolve excessive postpartum inflammation in some dams.